|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Antibacterial Effects of Chinese Herbal Medicines against Streptococcus iniae in Oreochromis niloticus显示文摘In this study,the antibacterial effects of Chinese herbal medicines against Streptococcus iniae in Oreochromis niloticus were investigated by drug sensitivity test. The results indicated that Streptococcus iniae was extremely sensitive to Galla Chinensis,Fructus Hippophae and Fructus Mume,highly sensitive to Sappan Lignum,Pericarpium Granati,Folium Eucalypti and Radix Scutellariae,moderately sensitive to Rhizoma Coptidis,Radix et Rhizoma Rhei,Flos Caryophyllata,Cortex phellodendri and Rabdosia serra( Maxim.) Hara,and insensitive to Herba Portulacae,Herba Houttuyniae,Polygonum hydropiper L.,Herba Menthae,Radix Glycyrrhizae,Pericarpium Citri Reticulatae,Herba Andrographitis and Rhizoma Acori Tatarinowii. Chinese medicinal herbs which Streptococcus iniae was extremely sensitive or moderately sensitive to were selected and prepared into three Chinese herbal medicine formulae for medicated bath of Oreochromis niloticus infected artificially with Streptococcus iniae. The results showed that medicated bath elicited therapeutic effects on infected Oreochromis niloticus. Formula II exhibited the best therapeutic effect with an effective percentage of 90%. | Guisheng GAO Yanying ZHANG Yongmei SU Qiumei SHI Guangping GAO Yating BAI Hongsheng HAN Jianxin ZHU Qingchun SONG Guanglian REN | 2016 | Agricultural Biotechnology2016，5，1： | 2 |
| 2 | Isolation and Identification of Photobacterium damselae subsp.damselae(PDD) from Tongue Sole显示文摘In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tissues of sick tongue sole aseptically collected.The isolate was identified as Photobacterium damselae subsp.damsela(PDD) by isolation culture,Gram staining,physiological identification,morohological observation,biochemical identification and 16S rDNA sequence analysis.The results showed that the isolate shared 99.6% homology with the reference strain in GenBank.The animal regression test displayed that the isolate had very strong pathogenicity to tongue sole.The LD_(50) was 3.1 × 10~4 CFU/mL,and it showed pathogenicity to mammals.The antimicrobial susceptibility test showed the isolate was highly sensitive to nrofloxacin,Norfloxacin,Ciprofloxacin,Mequindox;moderately sensitive to Cefradine,Doxycycline;and insensitive to Gentamicin,Ceftriaxone,Tilmicosin,etc.. | Yang Nan Zhang Zhiqiang Wu Tonglei Zhu Jianxin Fu Yanfang Han Hongsheng Wang Hongbin Shi Qiumei Gao Guisheng | 2018 | Animal Husbandry and Feed Science2018，10，2： | 2 |
| 3 | Effect of immunopotentiator of the Chinese herbal medicine on the quantity of T lymphocyte in chicken显示文摘In order to study the mechanism of immunopotentiator,the quantity of T lymphocyte was observed.Total 240 1-day chikens were divided into 3 groups randomly:one contrast group and two groups being drunk immunopotentiator according to the concentration 10 mL·L^(-1) and 5 mL·L^(-1) lasting for 48 d.The number of T lymphocyte in blood was measured by E-rosette when the chikens were at 12-,24-,36-,and 48-day.The results showed that the percent of T lymphocyte in the trial group was obviously higher than that of the contrast,and the 10 mL·L^(-1) group was higher than that of the 5 mL·L^(-1) group;the quantity distribution of T lymphocyte in intestinal mucosa lymphoid tissue of 14-day,21-day chiken was surveyed in the contrast group and the 10 mL·L^(-1) group with the method of routine histology-slices and ANAE,the results showed that the quantity of T lymphocyte in the 10 mL·L^(-1) group was significantly higher than that of the contrast,which indicated that immunopotentiator increased markedly the quantity of T lymphocyte,and the 10 mL·L^(-1) group was higher than that of the 5 mL·L^(-1) group. | SHI Qiumei SHEN Ping GAO Guisheng ZHAO Ruili TANG Shengling | 2007 | Journal of Northeast Agricultural University(English Edition)2007，14，2： | 1 |
| 4 | Particle Sizes and Antibacterial Activity of Radix Astragalus and Radix Isatidis Ultrafine Powders显示文摘Using 9200 laser particle size analyzer and KYKY-2800 scanning electron microscope,particle sizes and cellular morphology of Radix Astragalus and Radix Isatidis ultrafine powders were observed. According to the results,the particle size of 89.1% of Radix Astragalus ultrafine powders ranged from 1.729 μm to 44. 938 μm,D_(50)=4.368 μm; t he particle size of 93.411% of Radix Isatidis ultrafine powders ranged from 1. 510 μm to 44. 938 μm,D50= 8.726 μm. Radix Astragalus and Radix Isatidis ultrafine powders were pulverized completely without intact cellular morphology. The antibacterial activity of Radix Astragalus and Radix Isatidis ultrafine powders against chicken-derived E. coli(O78) w as investigated. The results indicated that Radix Astragalus and Radix Isatidis ultrafine powders exhibited higher antibacterial activity against chicken-derived E. coli(O78) c ompared with the corresponding coarse powders. This study laid a solid foundation for the development and application of Chinese medicine ultrafine powder preparations. | Qiumei SHI Xinhua SHAO Xiumin WANG Xia MENG Xiaoqiao HOU Baoxin YANG Leiyu GUO Jinglong GAO | 2016 | Agricultural Biotechnology2016，5，4： | 1 |
| 5 | In Vitro Antibacterial Effect of Traditional Chinese Medicine on Main Pathogenic Bacteria of Recessive Mastitis in Dairy Cows显示文摘The inhibition zone diameter,minimal inhibitory concentration( MIC) and minimum bactericidal concentration( MBC) of 20 kinds of traditional Chinese medicine on Staphylococcus aureus and Streptococcus agalactiae isolated from recessive mastitis were studied using plate test and tube test. The results showed that S. aureus and S. agalactiae were sensitive to all these 20 kinds of traditional Chinese medicine,and were more sensitive to seven kinds of traditional Chinese medicine,including Taraxacum mongolicum,Coptis chinensis,Forsythia suspense,Andrographis paniculata,Carthamus tinctorius,Scutellaria baicalensis and Dendranthema indicum. | Gao Guisheng Hu Tiefeng An Jiwei Gao Guangping Shi Qiumei Shao Xinhua Zhang Yanying | 2017 | Animal Husbandry and Feed Science2017，9，1： | 1 |
| 6 | Detection of High Pathogenicity Island(HPI) in Pathogenic Escherichia coli of Mink by PCR显示文摘[Objective] The paper was to analyze the carrying status of high pathogenicity island(HPI) in pathogenic Escherichia coli of mink.[Method] Eight strains of E. coli were isolated from dead mink, and conducted pathogenicity test of artificial infection. The carrying status of HPI(irp2, fyu A) was detected by PCR. [Result] Eight strains of E. coli were pathogenic E. coli, and the carrying rate of HPI(irp2, fyu A) was 100%,positively correlated with the pathogenicity. [Conclusion] The results lay a foundation for further exploring the pathogenic mechanism of E. coli.. | Xiao Lirong Li Qiaoling Gao Guisheng Jia Qinghui Zhang Zhaoxing Shi Qiumei | 2018 | Animal Husbandry and Feed Science2018，10，3： | 0 |
| 7 | Influences of Echinacea Polysaccharide on Secretion of IL-8mRNA by LPS-injured IEC-6 Cells显示文摘[Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8m RNA by LPS-injured IEC-6 cells,in order to figure out the mechanism of EPS on injured cells. [Method]Total RNA was extracted with TRIzon reagent. IL-8 m RNA was amplified by RT-PCR and detected by agarose gel electrophoresis. Furthermore,electrophoresis and image was analyzed. [Result] The 50 μg/m L EPS could partially inhibit IL-8 m RNA level produced by LPS-stimulated IEC-6; with the increasing concentration of EPS(200 and 500 μg/m L),the inhibitory effect against IL-8 m RNA gradually enhanced. IEC-6 was pretreated by 50,100,200 and 500 μg/m L EPS for 24 h,then stimulated by 10 μg/m L LPS for 1 and 4 h,respectively. RT-PCR analysis showed that the expression of IL-8 m RNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8m RNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at 1 h. [Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 m RNA by LPS-stimulated cells,and the inhibition of EPS was dependent to concentration and time. | Shi Qiumei Zhang Yanying Jia Qinghui Zhao Huiyuan Fu Yanfang Zhang Zhiqiang Wu Tonglei Hu Tiefeng An Jiwei | 2017 | Animal Husbandry and Feed Science2017，9，2： | 0 |
| 8 | Feasibility and physics potential of detecting ^(8)B solar neutrinos at JUNO显示文摘The Jiangmen Underground Neutrino Observatory(JUNO)features a 20 kt multi-purpose underground liquid scintillator sphere as its main detector.Some of JUNO's features make it an excellent location for^8B solar neutrino measurements,such as its low-energy threshold,high energy resolution compared with water Cherenkov detectors,and much larger target mass compared with previous liquid scintillator detectors.In this paper,we present a comprehensive assessment of JUNO's potential for detecting^8B solar neutrinos via the neutrino-electron elastic scattering process.A reduced 2 MeV threshold for the recoil electron energy is found to be achievable,assuming that the intrinsic radioactive background^(238)U and^(232)Th in the liquid scintillator can be controlled to 10^(-17)g/g.With ten years of data acquisition,approximately 60,000 signal and 30,000 background events are expected.This large sample will enable an examination of the distortion of the recoil electron spectrum that is dominated by the neutrino flavor transformation in the dense solar matter,which will shed new light on the inconsistency between the measured electron spectra and the predictions of the standard three-flavor neutrino oscillation framework.IfDelta m^(2)_(21)=4.8times10^(-5);(7.5times10^(-5))eV^(2),JUNO can provide evidence of neutrino oscillation in the Earth at approximately the 3sigma(2sigma)level by measuring the non-zero signal rate variation with respect to the solar zenith angle.Moreover,JUNO can simultaneously measureDelta m^2_(21)using^8B solar neutrinos to a precision of 20% or better,depending on the central value,and to sub-percent precision using reactor antineutrinos.A comparison of these two measurements from the same detector will help understand the current mild inconsistency between the value of Delta m^2_(21)reported by solar neutrino experiments and the KamLAND experiment. | Angel Abusleme Thomas Adam Shakeel Ahmad Sebastiano Aiello Muhammad Akram Nawab Ali Fengpeng An Guangpeng An Qi An Giuseppe Andronico Nikolay Anfimov Vito Antonelli Tatiana Antoshkina Burin Asavapibhop João Pedro Athayde Marcondes de André Didier Auguste Andrej Babic Wander Baldini Andrea Barresi Eric Baussan Marco Bellato Antonio Bergnoli Enrico Bernieri David Biare Thilo Birkenfeld Sylvie Blin David Blum Simon Blyth Anastasia Bolshakova Mathieu Bongrand Clément Bordereau Dominique Breton Augusto Brigatti Riccardo Brugnera Riccardo Bruno Antonio Budano Max Buesken Mario Buscemi Jose Busto Ilya Butorov Anatael Cabrera Hao Cai Xiao Cai Yanke Cai Zhiyan Cai Antonio Cammi Agustin Campeny Chuanya Cao Guofu Cao Jun Cao Rossella Caruso Cédric Cerna Jinfan Chang Yun Chang Pingping Chen Po-An Chen Shaomin Chen Shenjian Chen Xurong Chen Yi-Wen Chen Yixue Chen Yu Chen Zhang Chen Jie Cheng Yaping Cheng Alexander Chepurnov Davide Chiesa Pietro Chimenti Artem Chukanov Anna Chuvashova Gérard Claverie Catia Clementi Barbara Clerbaux Selma Conforti Di Lorenzo Daniele Corti Salvatore Costa Flavio Dal Corso Christophe De La Taille Jiawei Deng Zhi Deng Ziyan Deng Wilfried Depnering Marco Diaz Xuefeng Ding Yayun Ding Bayu Dirgantara Sergey Dmitrievsky Tadeas Dohnal Georgy Donchenko Jianmeng Dong Damien Dornic Evgeny Doroshkevich Marcos Dracos Frédéric Druillole Shuxian Du Stefano Dusini Martin Dvorak Timo Enqvist Heike Enzmann Andrea Fabbri Lukas Fajt Donghua Fan Lei Fan Can Fang Jian Fang Marco Fargetta Anna Fatkina Dmitry Fedoseev Vladko Fekete Li-Cheng Feng Qichun Feng Richard Ford Andrey Formozov Amélie Fournier Haonan Gan Feng Gao Alberto Garfagnini Alexandre Göttel Christoph Genster Marco Giammarchi Agnese Giaz Nunzio Giudice Franco Giuliani Maxim Gonchar Guanghua Gong Hui Gong Oleg Gorchakov Yuri Gornushkin Marco Grassi Christian Grewing Maxim Gromov Vasily Gromov Minghao Gu Xiaofei Gu Yu Gu Mengyun Guan Nunzio Guardone Maria Gul Cong Guo Jingyuan Guo Wanlei Guo Xinheng Guo Yuhang Guo Paul Hackspacher Caren Hagner Ran Han Yang Han Miao He Wei He Tobias Heinz Patrick Hellmuth Yuekun Heng Rafael Herrera Daojin Hong YuenKeung Hor Shaojing Hou Yee Hsiung Bei-Zhen Hu Hang Hu Jianrun Hu Jun Hu Shouyang Hu Tao Hu Zhuojun Hu Chunhao Huang Guihong Huang Hanxiong Huang Qinhua Huang Wenhao Huang Xingtao Huang Yongbo Huang Jiaqi Hui Wenju Huo Cédric Huss Safeer Hussain Antonio Insolia Ara Ioannisian Daniel Ioannisyan Roberto Isocrate Kuo-Lun Jen Xiaolu Ji Xingzhao Ji Huihui Jia Junji Jia Siyu Jian Di Jiang Xiaoshan Jiang Ruyi Jin Xiaoping Jing Cécile Jollet Jari Joutsenvaara Sirichok Jungthawan Leonidas Kalousis Philipp Kampmann Li Kang Michael Karagounis Narine Kazarian Amir Khan Waseem Khan Khanchai Khosonthongkee Patrick Kinz Denis Korablev Konstantin Kouzakov Alexey Krasnoperov Svetlana Krokhaleva Zinovy Krumshteyn Andre Kruth Nikolay Kutovskiy Pasi Kuusiniemi Tobias Lachenmaier Cecilia Landini Sébastien Leblanc Frederic Lefevre Liping Lei Ruiting Lei Rupert Leitner Jason Leung Demin Li Fei Li Fule Li Haitao Li Huiling Li Jiaqi Li Jin Li Kaijie Li Mengzhao Li Nan Li Nan Li Qingjiang Li Ruhui Li Shanfeng Li Shuaijie Li Tao Li Weidong Li Weiguo Li Xiaomei Li Xiaonan Li Xinglong Li Yi Li Yufeng Li Zhibing Li Ziyuan Li Hao Liang Hao Liang Jingjing Liang Jiajun Liao Daniel Liebau Ayut Limphirat Sukit Limpijumnong Guey-Lin Lin Shengxin Lin Tao Lin Jiajie Ling Ivano Lippi Fang Liu Haidong Liu Hongbang Liu Hongjuan Liu Hongtao Liu Hu Liu Hui Liu Jianglai Liu Jinchang Liu Min Liu Qian Liu Qin Liu Runxuan Liu Shuangyu Liu Shubin Liu Shulin Liu Xiaowei Liu Yan Liu Alexey Lokhov Paolo Lombardi Claudio Lombardo Kai Loo Chuan Lu Haoqi Lu Jingbin Lu Junguang Lu Shuxiang Lu Xiaoxu Lu Bayarto Lubsandorzhiev Sultim Lubsandorzhiev Livia Ludhova Fengjiao Luo Guang Luo Pengwei Luo Shu Luo Wuming Luo Vladimir Lyashuk Qiumei Ma Si Ma Xiaoyan Ma Xubo Ma Jihane Maalmi Yury Malyshkin Fabio Mantovani Francesco Manzali Xin Mao Yajun Mao Stefano MMari Filippo Marini Sadia Marium Cristina Martellini Gisele Martin-Chassard Agnese Martini Davit Mayilyan Axel Müller Ints Mednieks Yue Meng Anselmo Meregaglia Emanuela Meroni David Meyhöfer Mauro Mezzetto Jonathan Miller Lino Miramonti Salvatore Monforte Paolo Montini Michele Montuschi Nikolay Morozov Pavithra Muralidharan Massimiliano Nastasi Dmitry VNaumov Elena Naumova Igor Nemchenok Alexey Nikolaev Feipeng Ning Zhe Ning Hiroshi Nunokawa Lothar Oberauer Juan Pedro Ochoa-Ricoux Alexander Olshevskiy Domizia Orestano Fausto Ortica Hsiao-Ru Pan Alessandro Paoloni Nina Parkalian Sergio Parmeggiano Teerapat Payupol Yatian Pei Nicomede Pelliccia Anguo Peng Haiping Peng Frédéric Perrot Pierre-Alexandre Petitjean Fabrizio Petrucci Luis Felipe Piñeres Rico Oliver Pilarczyk Artyom Popov Pascal Poussot Wathan Pratumwan Ezio Previtali Fazhi Qi Ming Qi Sen Qian Xiaohui Qian Hao Qiao Zhonghua Qin Shoukang Qiu Muhammad Rajput Gioacchino Ranucci Neill Raper Alessandra Re Henning Rebber Abdel Rebii Bin Ren Jie Ren Taras Rezinko Barbara Ricci Markus Robens Mathieu Roche Narongkiat Rodphai Aldo Romani Bedřich Roskovec Christian Roth Xiangdong Ruan Xichao Ruan Saroj Rujirawat Arseniy Rybnikov Andrey Sadovsky Paolo Saggese Giuseppe Salamanna Simone Sanfilippo Anut Sangka Nuanwan Sanguansak Utane Sawangwit Julia Sawatzki Fatma Sawy Michaela Schever Jacky Schuler Cédric Schwab Konstantin Schweizer Dmitry Selivanov Alexandr Selyunin Andrea Serafini Giulio Settanta Mariangela Settimo Muhammad Shahzad Vladislav Sharov Gang Shi Jingyan Shi Yongjiu Shi Vitaly Shutov Andrey Sidorenkov FedorŠimkovic Chiara Sirignano Jaruchit Siripak Monica Sisti Maciej Slupecki Mikhail Smirnov Oleg Smirnov Thiago Sogo-Bezerra Julanan Songwadhana Boonrucksar Soonthornthum Albert Sotnikov Ondrej Sramek Warintorn Sreethawong Achim Stahl Luca Stanco Konstantin Stankevich DušanŠtefánik Hans Steiger Jochen Steinmann Tobias Sterr Matthias Raphael Stock Virginia Strati Alexander Studenikin Gongxing Sun Shifeng Sun Xilei Sun Yongjie Sun Yongzhao Sun Narumon Suwonjandee Michal Szelezniak Jian Tang Qiang Tang Quan Tang Xiao Tang Alexander Tietzsch Igor Tkachev Tomas Tmej Konstantin Treskov Andrea Triossi Giancarlo Troni Wladyslaw Trzaska Cristina Tuve Stefan van Waasen Johannes van den Boom Guillaume Vanroyen Nikolaos Vassilopoulos Vadim Vedin Giuseppe Verde Maxim Vialkov Benoit Viaud Cristina Volpe Vit Vorobel Lucia Votano Pablo Walker Caishen Wang Chung-Hsiang Wang En Wang Guoli Wang Jian Wang Jun Wang Kunyu Wang Lu Wang Meifen Wang Meng Wang Ruiguang Wang Siguang Wang Wei Wang Wenshuai Wang Xi Wang Xiangyue Wang Yangfu Wang Yaoguang Wang Yi Wang Yifang Wang Yuanqing Wang Yuman Wang Zhe Wang Zheng Wang Zhimin Wang Zongyi Wang Apimook Watcharangkool Lianghong Wei Wei Wei Yadong Wei Liangjian Wen Christopher Wiebusch Steven Chan-Fai Wong Bjoern Wonsak Diru Wu Fangliang Wu Qun Wu Wenjie Wu Zhi Wu Michael Wurm Jacques Wurtz Christian Wysotzki Yufei Xi Dongmei Xia Yuguang Xie Zhangquan Xie Zhizhong Xing Benda Xu Donglian Xu Fanrong Xu Jilei Xu Jing Xu Meihang Xu Yin Xu Yu Xu Baojun Yan Xiongbo Yan Yupeng Yan Anbo Yang Changgen Yang Huan Yang Jie Yang Lei Yang Xiaoyu Yang Yifan Yang Haifeng Yao Zafar Yasin Jiaxuan Ye Mei Ye Ugur Yegin Frédéric Yermia Peihuai Yi Xiangwei Yin Zhengyun You Boxiang Yu Chiye Yu Chunxu Yu Hongzhao Yu Miao Yu Xianghui Yu Zeyuan Yu Chengzhuo Yuan Ying Yuan Zhenxiong Yuan Ziyi Yuan Baobiao Yue Noman Zafar Andre Zambanini Pan Zeng Shan Zeng Tingxuan Zeng Yuda Zeng Liang Zhan Feiyang Zhang Guoqing Zhang Haiqiong Zhang Honghao Zhang Jiawen Zhang Jie Zhang Jingbo Zhang Peng Zhang Qingmin Zhang Shiqi Zhang Tao Zhang Xiaomei Zhang Xuantong Zhang Yan Zhang Yinhong Zhang Yiyu Zhang Yongpeng Zhang Yuanyuan Zhang Yumei Zhang Zhenyu Zhang Zhijian Zhang Fengyi Zhao Jie Zhao Rong Zhao Shujun Zhao Tianchi Zhao Dongqin Zheng Hua Zheng Minshan Zheng Yangheng Zheng Weirong Zhong Jing Zhou Li Zhou Nan Zhou Shun Zhou Xiang Zhou Jiang Zhu Kejun Zhu Honglin Zhuang Liang Zong Jiaheng Zou | 2021 | Chinese Physics C2021，45，2： | 0 |
| 9 | Histopathological Observation of Cynoglossus semilaevis Günther with Abdominal Dropsy in Industrial Aquaculture显示文摘Objective This study aimed to investigate and analyze the histopathological changes in various tissues and organs of Cynoglossus semilaevis Günther with abdominal dropsy. Method By HE staining,pathological tissues of main organs of C. semilaevis Günther with abdominal dropsy were observed. Result According to the results of histopathological observation,C. semilaevis Günther with abdominal dropsy exhibited a large amount of ascites in the abdominal cavity;small intestinal mucosal epithelial cells were denatured and necrotic,and shed into the enteric cavity,with submucosal vascular congestion; the liver and pancreas tissues were denatured and necrotic,and the morphology of most liver cells disappeared. However,there was no remarkable pathological change in the heart,spleen and other organs. Conclusion C. semilaevis Günther with abdominal dropsy exhibited serious pathological and histochemical lesions in the small intestine,liver and pancreas,causing metabolic disorders. | Guangping GAO Guisheng GAO Yanying ZHANG Zhixin JI Qinghui JIA Ting SU Qiumei SHI Hong-shengHAN Qingchun Song Jianxin ZHU | 2016 | Agricultural Biotechnology2016，5，1： | 0 |
| 10 | Diagnosis and Control of Rabbit Hemorrhagic Disease(RHD)显示文摘In this study,chronic-type rabbit hemorrhagic disease occurred in rabbit warrens of Shandong,Liaoning,Beijing and Hebei,which was mainly characterized by deaths of young and adult rabbits,longer durationand nerve symptoms,was surveyed by epidemiological investigation,bacterial isolation and incubation,artificial infection test and histopathological method to analyze the causes of chronic-type rabbit hemorrhagic disease and put forward the corresponding control measures. | Fang XIANG Rui GUO Hongxue ZHANG Fujin LI Changhao LIU Qiumei SHI Xinhua SHAO | 2016 | Agricultural Biotechnology2016，5，1： | 0 |
| 11 | PCR Detection of Streptococcus agalactiae in Dairy Cows Suffering from Mastitis显示文摘[Objective] This study aimed to identify Streptococcus agalactiae and lay the foundation for the prevention and control of mastitis in dairy cows. [Method]Ten strains were isolated from milk samples produced by diseased dairy cows suffering from mastitis for morphologic observation,culture characteristic investigation,biochemical identification and Lancefield grouping. The isolated strains were identified at the molecular level by nested-PCR. [Result] Among the ten isolates,six strains were β-hemolytic and Gram-positive on blood agar,belonging to Lancefield group B,which were identified as Streptococcus agalactiae by biochemical identification and nested-PCR. After overnight incubation,the coincidence rate between results of nested-PCR detection and biochemical identification reached 100%. [Conclusion] Bacterial incubation,rapid DNA extraction and specific PCR can provide basis for early epidemiological survey of Streptococcus agalactiae infection in cattle. | Yanying ZHANG Guisheng GAO Zhengben LI Guangping GAO Qiumei SHI Xinhua SHAO Yinju LIANG Huiran LIU | 2013 | Agricultural Biotechnology2013，2，5： | 0 |
| 12 | Screening of Chinese Herbal Medicines against Avian Infectious Laryngotracheitis Virus and Escherichia coli显示文摘[Objective]This study aimed to screen Chinese herbal medicines against avian infectious laryngotracheitis virus and Escherichia coli. [Method]Conventional Chinese medicine plate dilution method,plate punching method and test tube method were applied to screen Chinese herbal medicines against avian infectious laryngotracheitis virus based on chicken embryo inoculation experiment. [Result] Forsythia suspensa,Radix Isatidis,Isatis indigotica,Lonicera japonica, Codonopsis pilosula,Astragalus membranaceus,Rhizoma Atractylodis Macrocephalae,Glycyrrhiza uralensis and Pericarpium granati had relatively strong anti-ILTV effect; among the Chinese herbal medicines against avian E. coli,Sanguisorba officinalis,Fructus Mume,Rheum palmatum,Anemarrhena asphodeloides,Radix Scutellariae and Fagopyrum cymosum had relatively strong effect against avian E. coli O 8,while other Chinese herbal medicines had relatively weak or no inhibitory effect on avian E. coli O 8 and O 5. [Conclusion]This study laid the foundation for further development of Chinese herbal medicine compound preparations to treat avian infectious laryngotracheitis,avian colibacillosis and other viral diseases and bacterial diseases. | Yanying ZHANG Qiumei SHI Guisheng GAO Guangping GAO Ping SHEN | 2013 | Agricultural Biotechnology2013，2，5： | 0 |
| 13 | Histopathological Observation of Goose Escherichia coli Disease显示文摘[Objective]This study aimed to provide reliable basis for in-depth understanding of histopathological changes in geese suffering from Escherichia coli disease and accurate diagnosis of goose E. coli disease. [Method]By using paraffin embedding,section slicing and HE staining,histopathological changes in the heart,liver,spleen,lung,kidney and small intestine tissues of three diseased geese were observed. [Result]The liver generated protein fiber membranes,showing liver cell degeneration,increasing number of red blood cells in sinusoids and liver congestion; the kidney had relatively serious lesions,including renal tubular epithelial cell degeneration,necrosis and shedding from the basement membrane and hyaline degeneration of glomerulars. The lung showed epithelial cell degeneration and necrosis and was filled with a large number of red blood cells and shedded epithelial cells,showing typical red hepatization; a large number of red blood cells were aggregated in pulmonary blood vessels,resulting in lung congestion. Epithelial cells of various digestive tubes all showed varying degrees of minor shedding and local infiltration of inflammatory cells,thereby leading to death of geese due to substance metabolism,electrolyte and acid-base balance disorders and metabolic acidosis. [Conclusion] Based on epidemiology survey,clinical symptom observation,pathological necropsy and laboratory diagnosis,the diseased geese were confirmed to be suffering from E. coli disease. | Ping SHEN Guangping GAO Guisheng GAO Yanying ZHANG Qiumei SHI Xinhua SHAO Lining HE Wenwen SU | 2013 | Agricultural Biotechnology2013，2，5： | 0 |
| 14 | Histopathological Observation of Classical Swine Fever in Newborn Piglets显示文摘[Objective]This study aimed to investigate pathological changes in various organs of piglets infected with classical swine fever and provide reliable basis for accurate diagnosis of classical swine fever. [Method]By using paraffin embedding,section slicing and HE staining,histopathological changes in the heart, liver,spleen,lung,kidney and small intestine tissues of five diseased piglets were observed. [Result]Classical swine fever virus could lead to multiple visceral and mucosal hemorrhages,inflammatory cell infiltration,necrosis and other pathological changes. [Conclusion]Classical swine fever virus can further cause progressive degeneration and necrosis of different cells,resulting in immune injury. | Ping SHEN Guisheng GAO Yanying ZHANG Qiumei SHI Guangping GAO Zhipeng LI | 2013 | Agricultural Biotechnology2013，2，5： | 0 |
| 15 | Effects of Immunopotentiator of the Traditional Chinese Medicine on T Lymphocytes in Chicken Blood显示文摘In order to investigate the mechanism of action of immunoenhancer,the effects of the traditional Chinese medicine immunopromoter on the quantity and the transformation rates of T lymphocytes in the chicken blood were determined.Total 120 chickens were randomly assigned into three groups.The 1% and the 0.5% of the Chinese medicine immunopromoter were added to the chicken drinking water,respectively.The quantity of T lymphocytes in each group was measured by a-Naphthyl acetate esterase(ANAE) staining.The results showed that the percentages of T lymphocytes of the treatment groups were significantly higher than that of the control group(P<0.05),and the percentage of the 1% group significantly higher than that of the 0.5% group(P<0.05).In conclusion,the transformation rates of T lymphocytes showed that the Chinese medicine immunopromoter had the significant enhancing effect on the transformation rates of T lymphocytes of the treated chickens.The traditional Chinese medicine immunopromoter had the distinct function to promote the quantity and the transformation rate of T lymphocytes. | SHI Qiumei LI Chunling GAO Guisheng SHEN Ping TANG Shengling | 2008 | Journal of Northeast Agricultural University(English Edition)2008，15，2： | 0 |
| 16 | Effects of Echinacea Polysaccharide on Expression of NF-κB Protein Secreted by LPS-injured IEC-6 Cells显示文摘[Objective] The paper was to explore effects of Echinacea polysaccharide( EPS) on expression of NF-κB protein secreted by LPS-injured IEC-6 cells,and to provide a theoretical basis for clinical application of Echinacea purpurea against bacterial diseases and enhancement of immunity. [Method] Nucleoprotein extracted from IEC-6 cells in normal control group,LPS group,different concentrations of EPS( 50,100,200,500 μg/m L) + LPS groups were detected by SDSPAGE electrophoresis,and the content of NF-κB protein was analyzed using western blotting method. [Result] The content of NF-κB protein in normal control group was the lowest,while that in LPS group was the highest. The content of NF-κB protein in EPS group gradually decreased with the increasing concentration of EPS. [Result]The expression of NF-κB protein increased when IEC-6 cells were stimulated by LPS,and EPS could effectively inhibit increased expression of NF-κB protein. With the increasing concentration of EPS,the inhibition effect against increased expression of NF-κB protein gradually strengthened. | Shi Qiumei Zhang Yanying Jia Qinghui Gao Guisheng Gao Guangping Wang Jinfeng Zhang Donglin | 2017 | Animal Husbandry and Feed Science2017，9，1： | 0 |
| 17 | Isolation,Identification and Drug Tolerance Analysis of Pathogenic Staphylococcus aureus from Broilers显示文摘White feather broilers in some broiler farm in Chengde area developed arthritis and died acutely. In order to identify the pathogen inducing the disease and death of the white feather broilers,samples such as livers and joint pus were collected from dead broilers under sterile condition,and one pathogenic strain was isolated. The isolate was identified to be Staphylococcus aureus through isolated culture,morphologic observation,inspection of biochemical property and animal test. The drug sensitivity test showed that the isolate was sensitive to ceftazidime,enrofloxacin,ceftriaxone,lincomycin and amikacin,but resistant to other drugs to different degrees. | Tieying CHAI Lirong XIAO Zhaoxing ZHANG Guisheng GAO Qiumei SHI Yujiang ZHANG | 2018 | Agricultural Biotechnology2018，7，4： | 0 |
| 18 | Isolation and Identification of a Pathogenic E.coli Strain Causing Diarrhea in Foxes显示文摘[Objectives]The study aimed to identify the pathogenic E. coli strain that caused diarrhea in foxes and to analyze its drug sensitivity. [Methods]A pathogenic E. coli strain was isolated from dead foxes with diarrhea. By conventional bacterial isolation and culture,morphological observation,pathogenicity test and K-B disc method,the isolated strain was identified as pathogenic E. coli. [Results]The isolated pathogen was highly sensitive to ceftriaxone,cefotaxime,ciprofloxacin and lincomycin,moderately sensitive to enrofloxacin,neomycin,gentamycin,spectinomycin,florfenicol,amikacin and polymyxin,and resistant to ampicillin,amoxicillin and doxycycline. [Conclusions]This study provided reference for the prevention and control of diarrheal diseases in foxes in Qinhuangdao region. | Lirong XIAO Qiaoling LI Guisheng GAO Qinghui JIA Zhaoxing ZHANG Qiumei SHI | 2018 | Agricultural Biotechnology2018，7，4： | 0 |
| 19 | Effects of Two Drugs on Small Intestinal Epithelial Lymphocytes of Experimental Piglets with Spleen Deficiency显示文摘[Objective] This study aimed to investigate the effects of Guchang Cuzhang powder and Smecta on small intestinal epithelial lymphocytes of piglets with spleen deficiency and diarrhea induced by Reserpine. [Method] Eighteen piglets with reserpine-induced spleen deficiency and diarrhea were selected and randomly divided into three groups,six per group,including Guchang Cuzhang powder group,Smecta group and spleen deficiency control group. Piglets in two drug administration groups were treated for 7 d using Guchang Cuzhang powder and Smecta,respectively. On the 2ndd and 8thd after drug withdrawal,three piglets in each group were euthanized by jugular vein bleeding to death. Intestine segments in different groups were collected for paraffin embedding,section preparation and HE staining,to conduct histological observation and count the number of small intestinal epithelial lymphocytes. [Result] Compared with the spleen deficiency control group,the number of lymphocytes in small intestinal epithelium of piglets in two drug administration groups increased significantly; in addition,the number of lymphocytes in small intestinal epithelium of piglets in Guchang Cuzhang powder group was significantly higher than that in Smecta group. [Conclusion] This study provided theoretical basis for the clinical application of Guchang Cuzhang powder and Smecta. | Ping SHEN Yanying ZHANG Guisheng GAO Qiumei SHI Guangping GAO Xinhua SHAO | 2013 | Agricultural Biotechnology2013，2，4： | 0 |
| 20 | Effect of Echinacea Polysaccharide on Secretion of IkB-αmRNA by IEC-6 Injured by LPS显示文摘[Objective]This study was conducted to investigate the mechanism of Echinacea polysaccharide(EPS) in treatment of various bacterial infection and reduction of inflammation,so as to provide a theoretical basis for clinic application of EPS.[Method]Nuclear protein extracted from six groups,the normal control group,the simple lipopolysaccharide(LPS) group and the EPS(with concentrations of 50,100,200 and 500 μg/ml,respectively) + LPS groups was subjected to SDS-PAGE electrophoresis,and pIkB-α protein contents in the extracts were analyzed by Coomassie brilliant blue(CBB) staining and Western-Blot method.[Result]The simple LPS group showed the highest pIkB-α protein level,and in the EPS concentration range of 0-200 μg/ml,the expression level of pIkB-α protein was improved with the increase of EPS concentration.[Conclusion]The expression level of pIkB-α protein was improved under the simulation of IEC-6 by LPS,while EPS could effectively inhibit the expression of pIkB-α protein.The expression level of pIkB-α was the lowest in the LPS +500 μg/ml EPS group. | Qiumei SHI Yanying ZHANG Qinghui JIA Huiyuan ZHAO Minghe YU Guohui YU Jiwei An | 2017 | Agricultural Biotechnology2017，6，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员