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| 1 | Q-switching of waveguide lasers based on graphene/WS_2 van der Waals heterostructure显示文摘We report on the operation of passively Q-switched waveguide lasers at 1 μm wavelength based on a graphene∕WS_2 heterostructure as a saturable absorber(SA). The gain medium is a crystalline Nd:YVO_4 cladding waveguide produced by femtosecond laser writing. The nanosecond waveguide laser operation at 1064 nm has been realized with the maximum average output power of 275 m W and slope efficiency of 37%. In comparison with the systems based on single WS_2 or graphene SA, the lasing Q-switched by a graphene∕WS_2 heterostructure SA possesses advantages of a higher pulse energy and enhanced slope efficiency, indicating the promisingapplications of van der Waals heterostructures for ultrafast photonic devices. | ZIQI LI CHEN CHENG NINGNING DONG CAROLINA ROMERO QINGMING LU JUN WANG JAVIER RODRíGUEZ VáZQUEZ DE ALDANA YANG TAN FENG CHEN | 2017 | Photonics Research2017，5，5： | 7 |
| 2 | Monitor and control of neuronal activities with femtosecond pulse laser显示文摘Combined with the fluorescence labeling technique, two-photon microscopy excited with femtosecond pulse laser has become an important tool for neuroscience research. In this research, the calcium signals from neurons in rat cortex slice were monitored by a custom-built two-photon microscopy, and the spontaneous calcium signals and the pharmacological responses as well as the responses to femtosecond pulse laser stimulation were recorded. The results showed that the amplitude of the cal-cium signals increased in direct proportion to the corresponding electrical activities. Glutamate induced a calcium transient, but continuous application resulted in smaller response. Simultaneous monitoring of neuronal populations distinguished the neurons of different microcircuits. The femtosecond pulse laser induced local or global calcium signals in the pyramidal neurons. The approach of interrogation and control of neural activities using femtosecond pulse laser is non-contact, nondestructive, repeatable, and without any additional substrates, which will contribute to the development of neuroscience. | ZHOU Wei LIU XiuLi LU XiaoHua LI JiaSong LUO QingMing ZENG ShaoQun | 2008 | Chinese Science Bulletin2008，53，5： | 5 |
| 3 | Computational neuroscience:a frontier of the 21^st century显示文摘THEORY IN NEUROSCIENCE The human brain is a biological organ,weighing about three pounds or 1.4 kg,that determines our behaviors,thoughts,emotions and consciousness.Although comprising only 2%of the total body weight,the brain consumes about 20%of the oxygen entering the body.With the expensive energy demand,the brain enables us to perceive and act upon the external world,as well as reflect on our internal thoughts and feelings. | Xiao-Jing Wang Hailan Hu Chengcheng Huang Henry Kennedy Chengyu Tony Li Nikos Logothetis Zhong-Lin Lu Qingming Luo Mu-ming Poo Doris Tsao Si Wu Zhaohui Wu Xu Zhang Douglas Zhou | 2020 | National Science Review2020，7，9： | 4 |
| 4 | 2022 Chinese expert consensus and guidelines on clinical management of toxicity in anti-CD19 chimeric antigen receptor T-cell therapy for B-cell non-Hodgkin lymphoma显示文摘Adoptive cellular immunotherapy with chimeric antigen receptor(CAR)T cells has emerged as a novel modality for treating relapsed and/or refractory B-cell non-Hodgkin lymphoma(B-NHL).With increasing approval of CAR T-cell products and advances in CAR T cell therapy,CAR T cells are expected to be used in a growing number of cases.However,CAR T-cell-associated toxicities can be severe or even fatal,thus compromising the survival benefit from this therapy.Standardizing and studying the clinical management of these toxicities are imperative.In contrast to other hematological malignancies,such as acute lymphoblastic leukemia and multiple myeloma,anti-CD19 CAR T-cell-associated toxicities in B-NHL have several distinctive features,most notably local cytokine-release syndrome(CRS).However,previously published guidelines have provided few specific recommendations for the grading and management of toxicities associated with CAR T-cell treatment for B-NHL.Consequently,we developed this consensus for the prevention,recognition,and management of these toxicities,on the basis of published literature regarding the management of anti-CD19 CAR T-cell-associated toxicities and the clinical experience of multiple Chinese institutions.This consensus refines a grading system and classification of CRS in B-NHL and corresponding measures for CRS management,and delineates comprehensive principles and exploratory recommendations for managing anti-CD19 CAR T-cell-associated toxicities in addition to CRS. | Ping Li Yang Liu Yun Liang Jian Bo Sujun Gao Yongxian Hu Yu Hu He Huang Xiaojun Huang Hongmei Jing Xiaoyan Ke Jianyong Li Yuhua Li Qifa Liu Peihua Lu Heng Mei Ting Niu Yongping Song Yuqin Song Liping Su Sanfang Tu Jianxiang Wang Depei Wu Zhao Wang Kailin Xu Zhitao Ying Qingming Yang Yajing Zhang Fengxia Shi Bin Zhang Huilai Zhang Xi Zhang Mingfeng Zhao Weili Zhao Xiangyu Zhao Liang Huang Jun Zhu Wenbin Qian Weidong Han Aibin Liang | 2023 | Cancer Biology & Medicine2023，20，2： | 3 |
| 5 | Low intensity near-infrared light promotes bone regeneration via circadian clock protein cryptochrome 1显示文摘Bone regeneration remains a great clinical challenge. Low intensity near-infrared(NIR) light showed strong potential to promote tissue regeneration, offering a promising strategy for bone defect regeneration. However, the effect and underlying mechanism of NIR on bone regeneration remain unclear. We demonstrated that bone regeneration in the rat skull defect model was significantly accelerated with low-intensity NIR stimulation. In vitro studies showed that NIR stimulation could promote the osteoblast differentiation in bone mesenchymal stem cells(BMSCs) and MC3T3-E1 cells, which was associated with increased ubiquitination of the core circadian clock protein Cryptochrome 1(CRY1) in the nucleus. We found that the reduction of CRY1 induced by NIR light activated the bone morphogenetic protein(BMP) signaling pathways, promoting SMAD1/5/9 phosphorylation and increasing the expression levels of Runx2 and Osterix. NIR light treatment may act through sodium voltage-gated channel Scn4a, which may be a potential responder of NIR light to accelerate bone regeneration. Together, these findings suggest that low-intensity NIR light may promote in situ bone regeneration in a CRY1-dependent manner, providing a novel, efficient and non-invasive strategy to promote bone regeneration for clinical bone defects. | Jinfeng Peng Jiajia Zhao Qingming Tang Jinyu Wang Wencheng Song Xiaofeng Lu Xiaofei Huang Guangjin Chen Wenhao Zheng Luoying Zhang Yunyun Han Chunze Yan Qian Wan Lili Chen | 2022 | International Journal of Oral Science2022，14，4： | 2 |
| 6 | Visualizing DC morphology and T cell motility to characterize DC-T cell encounters in mouse lymph nodes under mTOR inhibition显示文摘Mammalian target of rapamycin(mTOR),a serine/threonine kinase orchestrating cellular metabolism,is a crucial immune system regulator.However,it remains unclear how mTOR regulates dendritic cell(DC) function in vivo,especially DC-T cell encounters,a critical step for initiating adaptive immune responses.We dynamically visualized DC-T contacts in mouse lymph node using confocal microscopy and established an encounter model to characterize the effect of mTOR inhibition on DC-T cell encounters using DC morphology.Quantitative data showed mTOR inhibition via rapamycin altered DC shape,with an increased form factor(30.17%) and decreased cellular surface area(20.36%) and perimeter(22.43%),which were associated with Cdc42 protein downregulation(52.71%).Additionally,DCs adopted a similar morphological change with Cdc42 inhibition via ZCL278 as that observed with mTOR inhibition.These morphologically altered DCs displayed low encounter rates with T cells.Time-lapse imaging data of T cell motility supported the simulated result of the encounter model,where antigen-specific T cells appeared to reduce arrest in the lymph nodes of rapamycin-pretreated mice relative to the untreated group.Therefore,mTOR inhibition altered DC morphology in vivo and decreased the DC-T cell encounter rate,as well as Cdc42 inhibition.By establishing an encounter model,our study provides an intuitive approach for the early prediction of DC function through morphological quantification of form factor and area. | Qiaoya Lin Zheng Liu Meijie Luo Hao Zheng Sha Qiao Chenlu Han Deqiang Deng Zhan Fan Yafang Lu Zhihong Zhang Qingming Luo | 2019 | Science China(Life Sciences)2019，62，9： | 2 |
| 7 | Blood flow activation in rat somatosensory cortex under sciatic nerve stimulation revealed by laser speckle imaging显示文摘In many functional neuroimaging research the change of local cerebral blood flow (CBF) induced by sensory stimulation is regarded as an indicator of the change in cortical neuronal activity although a precise and full spatio-temporal description of local CBF response coupled to neural activity has still not been laid out. Using the laser speckle imaging technique a relatively large exposed area in somatosensory cortex of rat was imaged for the observation of the variations of CBF during sciatic nerve stimulation. The results showed that cerebral blood flow activation was spatially localized and discretely distributed in the targeted microvasculature. Individual arteries, veins and capillaries in different diameters were activated with the time going. The response pattern of CBF related to the function of brain activity and energy metabolism is delineated exactly. | WANG Zheng, LUO Qingming, CHENG Haiying, LUO Weihua, GONG Hui and LU Qiang(The Key Laboratory of Biomedical Photonics of Ministry of Education of China, Huazhong University of Science and Technology, Wuhan 430074, China) | 2003 | Progress in Natural Science:Materials International2003，13，7： | 2 |
| 8 | Continuous wave-based multiphoton excitation fluorescence for capillary electrophoresis显示文摘 | Bi-Feng Liu Ling Fu Tao Xiong Tiancai Liu Zhihong Zhang Zhen-Li Huang Qiang Lu Yuan-Di Zhao Qingming Luo | 2006 | Journal of Chromatography A2006，1109，： | 1 |
| 9 | Glycerol-3-phosphate acyltransferase 4 gene is involved in mouse spermatogenesis显示文摘Glycerol-3-phosphate acyltransferase (GPAT ) 由把 glycerol-3-phosphate 变换成 lysophosphatidic 酸(LPA ) 催化 de novo triacylglycerol 合成的首先承诺的步。LPA 是调停的 mitogen 多重细胞的过程包括房间增长。四 GPAT isoforms 迄今为止被克隆。GPAT4 强烈在老鼠睾丸被表示。反向的 transcriptionpolymerase 链反应(PCR ) ,即时 PCR,并且在 situ,杂交(ISH ) 被用来分析 GPAT4 表达式并且在出生后的开发期间在鼠标睾丸本地化表示房间类型。GPAT4 cDNA 被插入到 pcDNA4/His 构造 recombinant 向量,它是进一根老鼠 spermatogonial 房间线(GC-1spg ) 的 transfected。GPAT4 首先出生后地在 2 个星期在老鼠被表示。表示从第三个星期是丰富的,在星期 56 点的 plateaued 然后在成年人在高水平维持了。ISH 表明那 GPAT4 基因在以后的精子形式期间在成熟分裂期间然而并非在伸长的 spermatids 在 spermatocytes 并且在 spermatids 附近富有地被表示。GC-1spg 房间与 GPAT4 在 transfection 以后在增长显示出显著增加;房间周期分析在在 S 阶段的 G0/G1 阶段和增加在房间的百分比显示出减少。因此, GPAT4 可能在精子发生起一个重要作用,在特别中间成熟分裂。 | Qingming Qiu Gang Liu Weina Li Qiuwen Shi Fuxi Zhu Guangxiu Lu | 2009 | Acta Biochimica et Biophysica Sinica2009，41，8： | 1 |
| 10 | Molecular identification of Anisakis and Hysterothylacium larvae in marine fishes from the East China Sea and the Pacific coast of central Japan显示文摘 | Qingming Kong Lanfen Fan Junhe Zhang Nobuaki Akao Kewei Dong Di Lou Jianzu Ding Qunbo Tong Bin Zheng Rui Chen Nobuo Ohta Shaohong Lu | 2015 | International Journal of Food Microbiology2015，，： | 1 |
| 11 | Characteristics of premanufacture CD8^(+) T cells determine CAR-T efficacy in patients with diffuse large B-cell lymphoma显示文摘Although chimeric antigen receptor(CAR)T cells have become an important treatment option for patients with relapsed/refractory B-cell malignancies,more than 60%of patients with diffuse large B-cell lymphoma(DLBCL)treated with CAR-T cell therapies fail to achieve a durable response.To reveal changes in CAR-T cell therapy and identify response biomarkers,we conducted a retrospective analysis of pre-manufacture source T cells and CAR-T cell products and their association with outcome in 58 patients with r/rDLBCL who received tandem CD19/CD20 CAR-T cell therapy.We performed bulk RNA-Seq,single-cell RNA-Seq,and paired T cell receptor sequencing on CAR-T cell products and pre-manufacture T cells from DLBCL patients.We note that a CD8+stem cell-like memory T cell population with a higher proportion and enhanced activating capacity of the CAR-T cell products was key to achieving durable clinical response.By analysing autologously-derived,pre-manufacture T cells,our data suggest that heterogeneity in the cellular and molecular features of pre-manufacture T cells contribute to the variation in efficacy after CAR-T cell therapy in DLBCL.The differences in anti-tumour efficacy of CAR-T cells among patients with different clinical outcomes appear to be due to the loss of CCR7 gene expression,coupled with increased expression of activation-and inhibitor-related genes in the CD8+naïve-T cell populations among the apheresis T cells from patients with a poor molecular response.These findings significantly advance our understanding of the underlying molecular determinants of pre-manufacture T cell function. | Yao Wang Chuan Tong Yuting Lu Zhiqiang Wu Yelei Guo Yang Liu Jianshu Wei Chunmeng Wang Qingming Yang Weidong Han | 2023 | Signal Transduction and Targeted Therapy2023，8，11： | 0 |
| 12 | The effect of rare earths on the solidification structure of Fe-36Ni invar alloy显示文摘The effect of Ce,La and mischmetal on the solidification structure of Fe-36Ni invar alloy was investigated.The results show that great amounts of high-melting point compounds (Ce2O3,La2O2S and (Ce,La)2O2S) respectively formed in the alloy with the addition of Ce,La or mischmetal.Based on the theory of lattice misfit,the lattice misfit between the (0001) surfaces of Ce2O3,Ce2O2S and La2O2S and (100) surface of Fe-36Ni invar alloy were 6.21%,5.77% and 5.42%,respectively,which are relatively low.Therefore,Ce2O3 ,La2O2S and (Ce,La)2O2S could serve as the core of heterogeneous nucleation,improve the equiaxed grain ratio,reduce the equiaxed grain size and refine the solidification structure of alloy. | LU Jiansheng LI Yuan LIU Qingming ZHENG Hongguang LIU Xufeng | 2012 | Baosteel Technical Research2012，6，3： | 0 |
| 13 | Origin of the Gas Hydrate and Free Gas in the Qilian Permafrost,Northwest China:Evidence from Molecular Composition and Carbon Isotopes显示文摘The Qilian permafrost of the South Qilian Basin(SQB)has become a research focus since gas hydrates were discovered in 2009.Although many works from different perspectives have been conducted in this area,the origin of gas from gas hydrate is still controversial.Molecular composition and carbon isotope of 190 samples related to gas hydrates collected from 11 boreholes allowed exploration of genetic type,thermal maturity,biodegradation,as well as gas-source correlation of alkane gases from gas hydrates and free gases.Results indicate that alkane gases biodegraded after the formation of natural gas.According to differences in carbon isotopes of methane and their congeners(CH4,C2H6,C3H8),the thermal maturity(vitrinite reflectance,VRo)of most alkane gases ranges from 0.6%to 1.5%,indicating a mature to high mature stage.The thermal maturity VRo of a small part of alkane gas(in boreholes DK5 and DK6)is higher than 1.3%,indicating a high mature stage.Alkane gases were mainly produced by secondary cracking,consisting of crude oilcracking gases and wet gases cracking to dry gases.Genetic types of alkane gases are primarily oil-type gases generated from shales and mudstones in the upper Yaojie Formation of Jurassic,with less coal-type gases originated from the mudstones in the Triassic Galedesi Formation and the lower Yaojie Formation of Jurassic.Carbon dioxides associated with alkanes from gas hydrates and free gases indicate the thermal decomposition and biodegradation of organic matter.The origins of natural gases from gas hydrates and free gases shed light on the evaluation of petroleum resource potential,deeply buried sediments,and petroleum resource exploration in the SQB. | TAN Furong LI Yang DU Fangpeng LIU Shiming CUI Weixiong LIU Zhiwu GENG Qingming LU Ping | 2021 | Acta Geologica Sinica(English Edition)2021，95，2： | 0 |
| 14 | Deep self-learning enables fast,high-fidelity isotropic resolution restoration for volumetric fluorescence microscopy显示文摘One intrinsic yet critical issue that troubles the field of fluorescence microscopy ever since its introduction is the unmatched resolution in the lateral and axial directions(i.e.,resolution anisotropy),which severely deteriorates the quality,reconstruction,and analysis of 3D volume images.By leveraging the natural anisotropy,we present a deep self-learning method termed Self-Net that significantly improves the resolution of axial images by using the lateral images from the same raw dataset as rational targets.By incorporating unsupervised learning for realistic anisotropic degradation and supervised learning for high-fidelity isotropic recovery,our method can effectively suppress the hallucination with substantially enhanced image quality compared to previously reported methods.In the experiments,we show that Self-Net can reconstruct high-fidelity isotropic 3D images from organelle to tissue levels via raw images from various microscopy platforms,e.g.,wide-field,laser-scanning,or super-resolution microscopy.For the first time,Self-Net enables isotropic whole-brain imaging at a voxel resolution of 0.2×0.2×0.2μm^(3),which addresses the last-mile problem of data quality in single-neuron morphology visualization and reconstruction with minimal effort and cost.Overall,Self-Net is a promising approach to overcoming the inherent resolution anisotropy for all classes of 3D fluorescence microscopy. | Kefu Ning Bolin Lu Xiaojun Wang Xiaoyu Zhang Shuo Nie Tao Jiang Anan Li Guoqing Fan Xiaofeng Wang Qingming Luo Hui Gong Jing Yuan | 2023 | Light(Science & Applications)2023，12，9： | 0 |
| 15 | A live attenuated RHΔompdcΔuprt mutant of Toxoplasma gondii induces strong protective immunity against toxoplasmosis in mice and cats显示文摘Background Toxoplasma gondii is an obligate intracellular apicomplexan parasite and is responsible for zoonotic toxoplasmosis.It is essential to develop an effective anti-T.gondii vaccine for the control of toxoplasmosis,and this study is to explore the immunoprotective effects of a live attenuated vaccine in mice and cats.Methods First,theompdc anduprt genes of T.gondii were deleted through the CRISPR-Cas9 system.Then,the intracellular proliferation and virulence of this mutant strain were evaluated.Subsequently,the immune responses induced by this mutant in mice and cats were detected,including antibody titers,cytokine levels,and subsets of T lymphocytes.Finally,the immunoprotective effects were evaluated by challenge with tachyzoites of different strains in mice or cysts of the ME49 strain in cats.Furthermore,to discover the effective immune element against toxoplasmosis,passive immunizations were carried out.GraphPad Prism software was used to conduct the log-rank(Mantel–Cox)test,Student’st test and one-way ANOVA.Results The RHΔompdcΔuprt were constructed by the CRISPR-Cas9 system.Compared with the wild-type strain,the mutant notably reduced proliferation(P<0.05).In addition,the mutant exhibited virulence attenuation in both murine(BALB/c and BALB/c-nu)and cat models.Notably,limited pathological changes were found in tissues from RHΔompdcΔuprt-injected mice.Furthermore,compared with nonimmunized group,high levels of IgG(IgG1 and IgG2a)antibodies and cytokines(IFN-γ,IL-4,IL-10,IL-2 and IL-12)in mice were detected by the mutant(P<0.05).Remarkably,all RHΔompdcΔuprt-vaccinated mice survived a lethal challenge with RHΔku80 and ME49 and WH6 strains.The immunized sera and splenocytes,especially CD8^(+)T cells,could significantly extend(P<0.05)the survival time of mice challenged with the RHΔku80 strain compared with naïve mice.In addition,compared with nonimmunized cats,cats immunized with the mutant produced high levels of antibodies and cytokines(P<0.05),and notably decreased the shedding numbers of oocysts in feces(95.3%).Conclusions The avirulent RHΔompdcΔuprt strain can provide strong anti-T.gondii immune responses,and is a promising candidate for developing a safe and effective live attenuated vaccine. | Yu Shen Bin Zheng Hao Sun Songrui Wu Jiyuan Fan Jianzu Ding Meng Gao Qingming Kong Di Lou Haojie Ding Xunhui Zhuo Shaohong Lu | 2023 | Infectious Diseases of Poverty2023，12，3： | 0 |
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