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Low intensity near-infrared light promotes bone regeneration via circadian clock protein cryptochrome 1

查看全文 作　　者：Jinfeng [1,2,3]Peng;Jiajia [1,2,3]Zhao;Qingming [1,2,3]Tang;Jinyu [2,3]Wang;Wencheng [1,2,3]Song;Xiaofeng [1,2,3]Lu;Xiaofei [1,2,3]Huang;Guangjin [1,2,3]Chen;Wenhao [1,2,3]Zheng;Luoying [4]Zhang;Yunyun [5]Han;Chunze [6]Yan;Qian [7]Wan;Lili [1,2,3]Chen 高影响力作者 机构地区：[1]Department of Stomatology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China;[2]School of Stomatology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China;[3]Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration,Wuhan,China;[4]Key Laboratory of Molecular Biophysics of Ministry of Education,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan,China;[5]Department of Neurobiology,School of Basic Medicine and Tongji Medical College,Huazhong University of Science&Technology,Wuhan,China;[6]State key Laboratory of Materials Processing and Die&Mould Technology,School of Materials Science and Engineering,Huazhong University of Science and Technology,Wuhan,China;[7]Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation,School of Pharmacy,Huazhong University of Science and Technology,Wuhan,China高影响力机构 出　　处：《International Journal of Oral Science》索引2022年第14卷第4期,共11页高影响力期刊 基　　金：funded by the National Key Research and Development Program of China(2021YFC2400404,to L.C.);the Key Program of National Natural Science of China(82030070,to L.C.);the National Science Foundation for Excellent Young Scholars of China(31725011,to L.C.);the Youth Clinical Research Fund of Chinese Stomatological Association(CSA-O2020-10,to Q.T.)。 摘　　要：Bone regeneration remains a great clinical challenge. Low intensity near-infrared(NIR) light showed strong potential to promote tissue regeneration, offering a promising strategy for bone defect regeneration. However, the effect and underlying mechanism of NIR on bone regeneration remain unclear. We demonstrated that bone regeneration in the rat skull defect model was significantly accelerated with low-intensity NIR stimulation. In vitro studies showed that NIR stimulation could promote the osteoblast differentiation in bone mesenchymal stem cells(BMSCs) and MC3T3-E1 cells, which was associated with increased ubiquitination of the core circadian clock protein Cryptochrome 1(CRY1) in the nucleus. We found that the reduction of CRY1 induced by NIR light activated the bone morphogenetic protein(BMP) signaling pathways, promoting SMAD1/5/9 phosphorylation and increasing the expression levels of Runx2 and Osterix. NIR light treatment may act through sodium voltage-gated channel Scn4a, which may be a potential responder of NIR light to accelerate bone regeneration. Together, these findings suggest that low-intensity NIR light may promote in situ bone regeneration in a CRY1-dependent manner, providing a novel, efficient and non-invasive strategy to promote bone regeneration for clinical bone defects. 关 键 词：STIMULATION INTENSITY LIGHT
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