|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Diagnosis of Helicobacter pylori : What should be the gold standard?显示文摘Since the discovery of Helicobacter pylori(H. pylori) in 1983, numerous detection methods for the presence of the bacterium have been developed. Each one of them has been associated with advantages and disadvantages. Noninvasive tests such as serology, 13 C urea breath test(UBT) and stool antigen tests are usually preferred by the clinicians. Serology has its own limitation especially in endemic areas while 13 C UBT is technically very demanding. The stool antigen detection method, although specific, is usually associated with poor sensitivity. The 13 C UBT is believed to be specific, but with present revelation of the fact that stomach is colonized by many other urease producing bacteria makes it questionable. Histology, culture, rapid urease test and polymerase chain reaction(PCR) are the tests which are carried out on antral biopsies collected by invasive means. Histology has been proposed to be very sensitive and specific but the question is how by simply looking the morphology of the bacteria in the microscope, one can claim that the curved bacterium is exclusively H. pylori. Rapid urease test(RUT), the doctor's test, is also challenged because the presence of other urease producing bacteria in the stomach cannot be denied. Moreover, RUT has been reported with poor sensitivity specially, when density of the bacterium is low. Isolation of H. pylori is essential to investigate its growth requirements, antibiotic susceptibility testing, studying virulence factor to develop vaccine and many more explorations. It has also got several disadvantages i.e., special condition for transporting, media, incubation and few days waiting for the colonies to appear, apart from the speed essentially needed to process the specimens. Till date, majority of the microbiological laboratories in the world are not equipped and trained to isolate such fastidious bacterium. The option left is PCR methods to detect H. pylori 's DNA in gastric mucosa, gastric juice, saliva, dental plaques and environmental specimens. There are speculations for false positivity due to detection of non-pylori Helicobacters due to genetic sharing; and false negativity due to low bacterial counts and presence of PCR inhibitors. However, specimen collection, transportation and processing do not require speed and special conditions. PCR based diagnosis may be considered as gold standard by designing primers extremely specific to H. pylori and targeting at least more than one conserved genes. Similarly specificity of PCR may be improved by use of internal Primers. Further, nested PCR will take care of false negatives by countering the effect of PCR inhibitors and low bacterial counts. Therefore, nested PCR based methods if performed properly, may be proposed as gold standard test. | Saurabh Kumar Patel Chandra Bhan Pratap Ashok Kumar Jain Anil Kumar Gulati Gopal Nath | 2014 | World Journal of Gastroenterology2014，20，36： | 30 |
| 2 | A review on synthesis of graphene,h-BN and MoS2 for energy storage applications:Recent progress and perspectives显示文摘The significance of graphene and its two-dimensional(2D)analogous inorganic layered materials especially as hexagonal boron nitride(h-BN)and molybdenum disulphide(MoS2)for“clean energy”applications became apparent over the last few years due to their extraordinary properties.In this review article we study the current progress and selected challenges in the syntheses of graphene,h-BN and MoS2 including energy storage applications as supercapacitors and batteries.Various substrates/catalysts(metals/insulator/semiconducting)have been used to obtain graphene,h-BN and MoS2 using different kinds of precursors.The most widespread methods for synthesis of graphene,h-BN and MoS2 layers are chemical vapor deposition(CVD),plasma-enhanced CVD,hydro/solvothermal methods,liquid phase exfoliation,physical methods etc.Current research has shown that graphene,h-BN and MoS2 layered materials modified with metal oxide can have an insightful influence on the performance of energy storage devices as supercapacitors and batteries.This review article also contains the discussion on the opportunities and perspectives of these materials(graphene,h-BN and MoS2)in the energy storage fields.We expect that this witen review article including recent research on energy storage will help in generating new insights for further development and practical applications of graphene,h-BN and MoS2 layers based materials. | Rajesh Kumar Sumanta Sahoo Ednan Joanni Rajesh Kumar Singh Ram Manohar Yadav Rajiv Kumar Verma Dinesh Pratap Singh Wai Kian Tan Angel Perez del Pino Stanislav A.Moshkalev Atsunori Matsuda | 2019 | Nano Research2019，12，11： | 12 |
| 3 | Comparison of phenotypic markers and neural differentiation potential of multipotent adult progenitor cells and mesenchymal stem cells显示文摘AIM: To compare the phenotypic and neural differentiation potential of human bone marrow derived multipotent adult progenitor cells (MAPC) and mesenchymal stem cells (MSC). METHODS: Cultures of MAPC and MSC were established in parallel from same samples of human bone marrow (n = 5). Both stem cell types were evaluated for expression of pluripotency markers including Oct-4 and Nanog by immunocytochemistry and reversetranscription polymerase chain reaction (RT-PCR) and expression of standard mesenchymal markers including CD14, CD34, CD44, CD45, CD73, CD90, CD105 andhuman leukocyte antigen (HLA)-ABC by flow cytometry. After treatment with neural induction medium both MAPC and MSC were evaluated for expression of neural proteins [neuronal filament-200 (NF-200) and glial fibrillar acidic protein (GFAP)] by immunocytochemistry and Western blotting and neural genes [NF-200, GFAP, Tau, microtubule-associated protein (MAP)-1B, MAP-2, neuron-specific enolase (NSE) and oligodendrocyte-1 (Olig-1)] by quantitative real-time-PCR. RESULTS: MAPC had small trigonal shaped while MSC had elongated spindle-shaped morphology. The MAPC expressed Oct-4 and Nanog both at gene and protein levels, whereas MSC were negative for these pluripotent markers. MAPC were negative for HLA-ABC while MSC had high expression of HLA-ABC. In addition, MAPC as compared to MSC had significantly lower expression of CD44 (36.56% ± 1.92% vs 98.23% ± 0.51%), CD73 (15.11% ± 2.24% vs 98.53% ± 2.22%) and CD105 (13.81% ± 3.82%vs 95.12% ± 5.65%) (P < 0.001, for all) MAPC cultures compared to MSC cultures treated with neural induction medium had significantly higher fold change expression of NF-200 (0.64), GFAP (0.52), Tau (0.59), MAP-2 (0.72), Olig-1 (0.18) and NSE (0.29) proteins (P < 0.01 for Olig-1 and P < 0.001 for rest) as well as higher fold change expression of genes of NF-200 (1.34),GFAP (1.12),Tau (1.08),MAP-1B (0.92), MAP-2 (1.14) andNSE (0.4) (P < 0.001 for all). CONCLUSION: MAPC can be differentially characterized from MSC as Oct-4 and Nanog positive stem cells with no expression of HLA-ABC and low expression of mesenchymal markers CD44, CD73 and CD105 and when compared to MSC they possess greater predilection for differentiation into neuro-ectodermal lineage. | Saurabh Pratap Singh Naresh Kumar Tripathy Soniya Nityanand | 2013 | World Journal of Stem Cells2013，5，2： | 10 |
| 4 | Structural characterization of monoterpene indole alkaloids in ethanolic extracts of Rauwolfia species by liquid chromatography with quadrupole time-of-flight mass spectrometry显示文摘Rauwolfia species(Apocynaceae) are medicinal plants well known worldwide due to its potent bioactive monoterpene indole alkaloids(MIAs) such as reserpine,ajmalicine,ajmaline,serpentine and yohimbine.Reserpine,ajmalicine and ajmaline are powerful antihypertensive,tranquilizing agents used in hypertension.Yohimbine is an aphrodisiac used in dietary supplements.As there is no report on the comparative and comprehensive phytochemical investigation of the roots of Rauwolfia species,we have developed an efficient and reliable liquid chromatography-tandem mass spectrometry(LC–MS/MS) method for ethanolic root extract of Rauwolfia species to elucidate the fragmentation pathways for dereplication of bioactive MIAs using highperformance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry(HPLC–ESI–QTOF–MS/MS) in positive ion mode.We identified and established diagnostic fragment ions and fragmentation pathways using reserpine,ajmalicine,ajmaline,serpentine and yohimbine.The MS/MS spectra of reserpine,ajmalicine,and ajmaline showed C-ring-cleavage whereas E-ring cleavage was observed in serpentine via Retro Diels Alder(RDA).A total of 47 bioactive MIAs were identified and characterized on the basis of their molecular formula,exact mass measurements and MS/MS analysis.Reserpine,ajmalicine,ajmaline,serpentine and yohimbine were unambiguously identified by comparison with their authentic standards and other 42 MIAs were tentatively identified and characterized from the roots of Rauwolfia hookeri,Rauwolfia micrantha,Rauwolfia serpentina,Rauwolfia verticillata,Rauwolfia tetraphylla and Rauwolfia vomitoria.Application of LC–MS followed by principal component analysis(PCA) has been successfully used to discriminate among six Rauwolfia species. | Sunil Kumar Awantika Singh Vikas Bajpai Mukesh Srivastava Bhim Pratap Singh Brijesh Kumar | 2016 | Journal of Pharmaceutical Analysis2016，6，6： | 7 |
| 5 | Soil Carbon Dynamics Under Changing Climate A Research Transition from Absolute to Relative Roles of Inorganic Nitrogen Pools and Associated Microbial Processes:A Review显示文摘It is globally accepted that soil carbon(C) dynamics are at the core of interlinked environmental problems, deteriorating soil quality and changing climate. Its management remains a complex enigma for the scientific community due to its intricate relationship with soil nitrogen(N) availability and moisture-temperature interactions. This article reviews the management aspects of soil C dynamics in light of recent advances, particularly in relation to the availability of inorganic N pools and associated microbial processes under changing climate. Globally, drastic alterations in soil C dynamics under changing land use and management practices have been primarily attributed to the variation in soil N availability, resulting in a higher decomposition rate and a considerable decline in soil organic C(SOC) levels due to increased soil CO_2 emissions, degraded soil quality, and increased atmospheric CO_2 concentrations,leading to climate warming. Predicted climate warming is proposed to enhance SOC decomposition, which may further increase soil N availability, leading to higher soil CO_2 efflux. However, a literature survey revealed that soil may also act as a potential C sink, if we could manage soil inorganic N pools and link microbial processes properly. Studies also indicated that the relative, rather than the absolute, availability of inorganic N pools might be of key importance under changing climate, as these N pools are variably affected by moisture-temperature interactions, and they have variable impacts on SOC turnover. Therefore, multi-factorial studies are required to understand how the relative availability of inorganic N pools and associated microbial processes may determine SOC dynamics for improved soil C management. | pratap srivastava rishikesh singh sachchidanand tripathi pardeep singh shikha singh hema singh akhilesh singh raghubanshi pradeep kumar mishra | 2017 | Pedosphere2017，27，5： | 5 |
| 6 | Effects of 42-year long-term fertilizer management on soil phosphorus availability, fractionation,adsorption–desorption isotherm and plant uptake in flooded tropical rice显示文摘Soil phosphorus(P) fractionation, adsorption, and desorption isotherm, and rice yield and P uptake were investigated in flooded tropical rice(Oryza sativa L.) following 42-year fertilizer and manure application. The treatments included low-input [unfertilized control without N, P, or K(C0N0)], farmyard manure(FYM)(C1N0), NP(C0NP), NPK(C0NPK), FYM + NP(C1NP), and high-input treatment, FYM + NPK(C1NPK). Grain yield was increased significantly by 74%over the control under the combined application of FYM + NPK. However, under low- and high-input treatments, yield as well as P uptake was maintained at constant levels for 35 years.During the same period, high yield levels and P uptake were maintained under the C0 NP, C0 NPK,and C1 NPK treatments. These are unique characteristics of a tropical flooded ecosystem, which is a self-sustaining system for rice production. The Fe–P fraction was highest compared to the Ca–P and Al–P fractions after 42 years of fertilizer application and was significantly higher under FYM + NPK treatment. The P adsorption capacity of soil was highest under the low-input treatment and lowest under long-term balanced fertilization(FYM + NPK). In contrast, P desorption capacity was highest under NPK and lowest in the control treatment. Long-term balanced fertilization in the form of FYM + NPK for 42 years lowered the bonding energy and adsorption capacity for P in soil but increased its desorption potential, increasing P availability to the plant and leading to higher P uptake and yield maintenance. | Pratap Bhattacharyya Amaresh Kumar Nayak Mohammad Shahid Rahul Tripathi Sangita Mohanty Anjani Kumar Rajagounder Raja Bipin Bihari Panda Banwari Lal Priyanka Gautam Chinmaya Kumar Swain Koushik Singha Roy Pradeep Kumar Dash | 2015 | The Crop Journal2015，3，5： | 5 |
| 7 | Pseudomonas fluorescens-like bacteria from the stomach:A microbiological and molecular study显示文摘AIM:To characterize oxidase-and urease-producing bacterial isolates,grown aerobically,that originated from antral biopsies of patients suffering from acid peptic diseases.METHODS:A total of 258 antral biopsy specimens were subjected to isolation of bacteria followed by tests for oxidase and urease production,acid tolerance and aerobic growth.The selected isolates were further characterized by molecular techniques viz.amplifications for 16S rRNA using universal eubacterial and HSP60 gene specific primers.The amplicons were subjected to restriction analysis and partial sequencing.A phylogenetic tree was generated using unweighted pair group method with arithmetic mean(UPGMA) from evolutionary distance computed with bootstrap test of phylogeny.Assessment of acidity tolerance of bacteria isolated from antrum was performed using hydrochloric acid from 10-7 mol/L to 10-1 mol/L.RESULTS:Of the 258 antral biopsy specimens collected from patients,179(69.4%) were positive for urease production by rapid urease test and 31%(80/258) yielded typical Helicobacter pylori(H.pylori) after 5-7 d of incubation under a microaerophilic environment.A total of 240(93%) antral biopsies yielded homogeneous semi-translucent and small colonies after overnight incubation.The partial 16S rRNA sequences revealed that the isolates had 99% similarity with Pseudomonas species.A phylogenetic tree on the basis of 16S rRNA sequences denoted that JQ927226 and JQ927227 were likely to be related to Pseudomonas fluorescens(P.fluorescens).On the basis ofHSP60 sequences applied to the UPGMA phylogenetic tree,it was observed that isolated strains in an aerobic environment were likely to be P.fluorescens,and HSP60 sequences had more discriminatory potential rather than 16S rRNA sequences.Interestingly,this bacterium was acid tolerant for hours at low pH.Further,a total of 250(96.9%) genomic DNA samples of 258 biopsy specimens and DNA from 240 bacterial isolates were positive for the 613 bp amplicons by targeting P.fluorescens-specific conserved putative outer membrane protein gene sequences.CONCLUSION:This study indicates that bacterial isolates from antral biopsies grown aerobically were P.fluorescens,and thus acid-tolerant bacteria other than H.pylori can also colonize the stomach and may be implicated in pathogenesis/protection. | Saurabh Kumar Patel Chandra Bhan Pratap Ajay Kumar Verma Ashok Kumar Jain Vinod Kumar Dixit Gopal Nath | 2013 | World Journal of Gastroenterology2013，19，7： | 4 |
| 8 | Achievements and prospects of grass pea(Lathyrus sativus L.) improvement for sustainable food production显示文摘Grass pea offers an attractive choice for sustainable food production, owing to its intrinsic properties including limited water requirement and drought tolerance. However, low productivity and the presence of a neurotoxin(ODAP) have posed major obstacles to its genetic improvement. Also, biotechnological investments remain limited and the genome is complex and not well understood. Strategies that allow identification of genotypes with reduced ODAP content, coupling of low ODAP content with enhanced yield, and effective seed detoxification methods merit immediate attention. Breeder-friendly genomic tools are being increasingly made available to improve the efficiency of breeding protocols. To this end, the application of next-generation sequencing has provided a means of leveraging the repertoire of genomic resources for this somewhat neglected crop. In this review, we describe progress achieved in Lathyrus genetic improvement. We also explore potential opportunities in Lathyrus research and identify urgent research needs. | Girish Prasad Dixit Ashok Kumar Parihar Abhishek Bohra Narendra Pratap Singh | 2016 | The Crop Journal2016，4，5： | 3 |
| 9 | 超导纳米线单光子探测器件的制备显示文摘超导纳米线单光子探测器件(SNSPD)是超导单光子探测系统的核心器件。文中介绍了成功制备的基于5nm厚的NbN超导超薄薄膜的SNSPD器件。器件核心结构为150nm宽的纳米曲折线结构,纳米线条占空比为75%,面积为20μm×20μm。超导纳米线是利用电子束曝光(EBL)技术和反应离子刻蚀(RIE)等工艺技术制备的。所制备的SNSPD样品,在温度3.5K下的临界电流约12.9μA;在1310nm波长光波辐照,12.5μA的偏置电流下,探测效率约0.016%。 | 杨晓燕 尤立星 张礼杰 黄少铭 Mintu Mondal Sanjeev Kumar John Jesudasan Pratap Raychaudhuri | 2010 | 低温与超导2010，，11： | 3 |
| 10 | A review of laser scanning for geological and geotechnical applications in underground mining显示文摘Laser scanning can provide timely assessments of mine sites despite adverse challenges in the operational environment.Although there are several published articles on laser scanning,there is a need to review them in the context of underground mining applications.To this end,a holistic review of laser scanning is presented including progress in 3D scanning systems,data capture/processing techniques and primary applications in underground mines.Laser scanning technology has advanced significantly in terms of mobility and mapping,but there are constraints in coherent and consistent data collection at certain mines due to feature deficiency,dynamics,and environmental influences such as dust and water.Studies suggest that laser scanning has matured over the years for change detection,clearance measurements and structure mapping applications.However,there is scope for improvements in lithology identification,surface parameter measurements,logistic tracking and autonomous navigation.Laser scanning has the potential to provide real-time solutions but the lack of infrastructure in underground mines for data transfer,geodetic networking and processing capacity remain limiting factors.Nevertheless,laser scanners are becoming an integral part of mine automation thanks to their affordability,accuracy and mobility,which should support their widespread usage in years to come. | Sarvesh Kumar Singh Bikram Pratap Banerjee Simit Raval | 2023 | International Journal of Mining Science and Technology2023，33，2： | 2 |
| 11 | Helicobacter pylori Is Not Eradicated after Triple Therapy: A Nested PCR Based Study显示文摘 | Saurabh Kumar Patel Girish Narayan Mishra Chandra Bhan Pratap Ashok Kumar Jain Gopal Nath Kathleen Montone | 2014 | BioMed Research International2014，，： | 2 |
| 12 | Distribution of geochemical fractions of phosphorus and its ecological risk in sediment cores of a largest brackish water lake, South Asia显示文摘The current study focuses on the vertical profile of different geochemical fractions of phosphorus-loosely bound(Lo–P),aluminium bound(Al–P),iron bound(Fe–P),calcium bound(Ca–P),and organic bound phosphorus(O–P)along with ecological risk assessment of sediment cores from Chilika Lake,eastcoast of India.The percentage contribution of the different fractions to the sedimentary phosphorus in the sediment column of the whole lake are on the order:O–P(33.2%)>Ca–P(20.3%)>Fe–P(18%)>Al–P(6.7%)>Lo–P(0.35%).The Phosphorus Pollution Index(PPI)revealed the contamination of lake sediment with phosphorus.The principal component and cluster analyses highlighted the anthropogenic contribution of phosphorus.The negative loading of Ca–P with Ca points towards its origin from marine shells.The discriminate analysis showed that the variables like Ca–P,bio-available phosphorus(BAP),and pH were able to effectively discriminate the sectors in a significant manner. | Saroja Kumar Barik Satyanarayan Bramha Tapan Kumar Bastia Dibakar Behera Pratap Kumar Mohanty Prasanta Rath | 2019 | International Journal of Sediment Research2019，34，3： | 2 |
| 13 | Draft genome sequence of a less-known wild Vigna: Beach pea(V. marina cv. ANBp-14-03)显示文摘Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family Fabaceae.It is a close relative of cultivated Vigna species such as adzuki bean(V.angularis),cowpea(V.unguiculata),mung bean(V.radiata),and blackgram(V.mungo),and is distributed throughout the tropics.With its ability to tolerate salt stress,beach pea has great potential to contribute salt-tolerance genes for developing salt-tolerant cultivars in cultivated Vigna species.However,it is still underutilized in Vigna breeding programs.A draft genome sequence of beach pea was generated using a high-throughput next-generation sequencing platform,yielding 23.7 Gb of sequence from 79,929,868 filtered reads.A de novo genome assembly containing 68,731 scaffolds gave an N50 length of 10,272 bp and the assembled sequences totaled 365.6 Mb.A total of 35,448 SSRs,including 3574 compound SSRs,were identified and primer pairs for most of these SSRs were designed.Genome analysis identified 50,670 genes with mean coding sequence length 1042 bp.Phylogenetic analysis revealed highest sequence similarity with V.angularis,followed by V.radiata.Comparison with the V.angularis genome revealed 16,699 SNPs and 2253 InDels and comparison with the V.radiata genome revealed 17,538 SNPs and 2300 InDels.To our knowledge this is the first draft genome sequence of beach pea derived from an accession(ANBp-14-03)adapted locally in the Andaman and Nicobar Islands of India.The draft genome sequence may facilitate the genetic enhancement in cultivated Vigna species. | Awnindra Kumar Singh A.Velmurugan Debjyoti Sen Gupta Jitendra Kumar Ravi Kesari Aravind Konda Narendra Pratap Singh Sibnarayan Dam Roy Utpal Biswas R.Rahul Kumar Sanjay Singh | 2019 | The Crop Journal2019，7，5： | 2 |
| 14 | SO2resistant antimony promoted V2O5/TiO2 catalyst for NH3-SCR of NOx at low temperatures显示文摘 | Ha Heon Phil Maddigapu Pratap Reddy Pullur Anil Kumar | 2008 | Applied Catalysis B: Environmental2008，78，34： | 1 |
| 15 | Combustion characteristics of diesel HCCI engine: An experimental investigation using external mixture formation technique显示文摘 | Akhilendra Pratap Singh Avinash Kumar Agarwal | 2012 | Applied Energy2012，，： | 1 |
| 16 | Simplex and duplex PCR assays for species specific identification of cattle and buffalo milk and cheese显示文摘 | Sachinandan De Biswajit Brahma Shamik Polley Ayan Mukherjee Deepak Banerjee Moloya Gohaina Karan Pratap Singh Rameswar Singh Tirtha Kumar Datta Surender Lal Goswami | 2010 | Food Control2010，，5： | 1 |
| 17 | Direct shoot regeneration from leaf explants of Rosa damascena Mill.显示文摘 | Pratap Kumar Pati Madhu Sharma Anil Sood P. S. Ahuja | 2004 | In Vitro Cellular & Developmental Biology - Plant2004，，2： | 1 |
| 18 | Application of a plain abdominal radiograph transition zone ( PARTZ ) in Hirschsprung' s disease 显示文摘 | Pratap A Agrawal CS Kumar A | 2007 | BMC Pediatr2007，7，： | 1 |
| 19 | Micropropagation, protoplast culture and ite implications in the improvement of scented rose 显示文摘 | Pratap Kumar Pati Madhu Sharma P S Ahula | 2001 | Acta Hort2001，547，： | 1 |
| 20 | The role of earthworm Lampito mauritii (Kinberg) in amending lead and zinc treated soil 显示文摘 | Sulata Maity Pratap Kumar Padhy Shibani Chaudhury | 2008 | Bioresource Technology2008，99，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员