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| 1 | First dark matter search results from the PandaX-Ⅰ experiment显示文摘We report on the first dark-matter(DM)search results from PandaX-I,a low threshold dual-phase xenon experiment operating at the China JinPing Underground Laboratory.In the 37-kg liquid xenon target with 17.4 live-days of exposure,no DM particle candidate event was found.This result sets a stringent limit for low-mass DM particles and disfavors the interpretation of previously-reported positive experimental results.The minimum upper limit,3.7×10-44cm2,for the spin-independent isoscalar DM-particle-nucleon scattering cross section is obtained at a DM-particle mass of 49 GeV/c2at 90%confidence level. | XIAO MengJiao XIAO Xiang ZHAO Li CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HU Jie HUANG XingTao JI XiangDong JU YongLin LEI SiAo LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing STEPHENSON Scott TAN AnDi TARL Greg WANG HongWei WANG JiMin WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIE PengWei YOU YingHui ZENG XiongHui ZHANG Hua ZHANG Tao ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，11： | 10 |
| 2 | PandaX: a liquid xenon dark matter experiment at CJPL显示文摘PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay search.The central vessel was designed to accommodate a staged target volume increase from initially 120 kg(stage I)to 0.5 t(stage II)and eventually to a multi-ton scale.The experiment is located in the Jinping Deep-Underground Laboratory in Sichuan,China.The detector operates in dual-phase mode,allowing detection of both prompt scintillation,and ionization charge through proportional scintillation.In this paper a detailed description of the stage I detector design and performance as well as results established during the commissioning phase are presented. | CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HE Ming HU Jie HUANG XingTao JI XiangDong JU YongLin LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing SHI YuJie STEPHENSON Scott TAN AnDi TARLé Greg WANG HongWei WANG JiMing WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIAO MengJiao XIAO Xiang XIE PengWei YE Tao YOU YingHui ZEN XiongHui ZHANG Hua ZHANG Tao ZHAO HaiYing ZHAO Li ZHOU XiaoPeng ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，8： | 9 |
| 3 | Differential Characteristics of the Upper Ordovician-Lower Silurian Wufeng-Longmaxi Shale Reservoir and its Implications for Exploration and Development of Shale Gas in/around the Sichuan Basin显示文摘The Upper Ordovician Wufeng-Lower Silurian Longmaxi shale is widely distributed in the Sichuan Basin and its periphery,which is the key stratum for marine shale gas exploration and development(E&D)in China.Based on sedimentary environment,material basis,storage space,fracability and reservoir evolution data,the reservoir characteristics of the Wufeng-Longmaxi shale and their significance for shale gas E&D are systematically compared and analyzed in this paper.The results show that(1)the depocenter of the Wufeng(WF)-Longmaxi(LM)shale gradually migrates from east to west.The high-quality shale reservoirs in the eastern Sichuan Basin are mainly siliceous shales,which are primarily distributed in the graptolite shale interval of WF2-LM5.The high-quality reservoirs in the southern Sichuan Basin are mainly calcareous-siliceous and organic-rich argillaceous shales,which are distributed in the graptolite shale interval of WF2-LM7.(2)Deep shale gas(the burial depth>3500 m)in the Sichuan Basin has high-ultrahigh pressure and superior physical properties.The organic-rich siliceous,calcareous-siliceous and organic-rich argillaceous shales have suitable reservoir properties.The marginal area of the Sichuan Basin has a higher degree of pressure relief,which leads to the argillaceous and silty shales evolving into direct cap rocks with poor reservoir/good sealing capacity.(3)Combining shale gas exploration practices and impacts of lithofacies,depth,pressure coefficient and brittle-ductile transition on the reservoir properties,it is concluded that the favorable depth interval of the Wufeng-Longmaxi shale gas is 2200~4000 m under current technical conditions.(4)Aiming at the differential reservoir properties of the Wufeng-Longmaxi shale in the Sichuan Basin and its periphery,several suggestions for future research directions and E&D of shale gas are formulated. | WANG Ruyue HU Zongquan LONG Shengxiang LIU Guangxiang ZHAO Jianhua DONG Li DU Wei WANG Pengwei YIN Shuai | 2019 | Acta Geologica Sinica(English Edition)2019，93，3： | 8 |
| 4 | Neomycin biosynthesis is regulated positively by AfsA-g and NeoR in Streptomyces fradiae CGMCC 4.7387显示文摘Neomycins are a group of aminoglycoside antibiotics with both clinical and agricultural applications.To elucidate the regulatory mechanism of neomycin biosynthesis,we completed draft genome sequencing of a neomycin producer Streptomyces fradiae CGMCC 4.7387 from marine sediments,and the neomycin biosynthesis gene cluster was identified.Inactivation of the afsA-g gene encoding a γ-butyrolactone(GBL) synthase in S.fradiae CGMCC 4.7387 resulted in a significant decrease of neomycin production.Quantitative RT-PCR analysis revealed that the transcriptional level of neoR and the aphA-neoGH operon were reduced in the afsA-g::aac(3)Ⅳ mutant.Interestingly,a conserved binding site of AdpA,a key activator in the GBL regulatory cascade,was discovered upstream of neoR,a putative regulatory gene encoding a protein with an ATPase domain and a tetratricopeptide repeat domain.When neoR was inactivated,the neomycin production was reduced about 40%in comparison with the WT strain.Quantitative RT-PCR analysis revealed that the transcriptional levels of genes in the aphA-neoGH operon were reduced clearly in the neoR::aac(3)Ⅳ mutant.Finally,the titers of neomycin were improved considerably by overexpression of qfsA-gand neoR in S.fradiae CGMCC 4.7387. | Xiangxi Meng Wenzhao Wang Zhoujie Xie Pengwei Li Yue Li Zhengyan Guo Yingjian Lu Jie Yang Kaile Guan Zhaoxin Lu Huarong Tan Yihua Chen | 2017 | Science China(Life Sciences)2017，60，9： | 7 |
| 5 | Global maximum power point tracking for solar power systems using the hybrid artificial fish swarm algorithm显示文摘Maximum power point tracking(MPPT) techniques are used to maintain photovoltaic modules operating points at the local maximum power points under non-uniform irradiance conditions(NUIC). For global maximum power point tracking(GMPPT) within an appropriate period, a hybrid artificial fish swarm algorithm(HAFSA) is proposed in this paper, which was developed using particle swarm optimization(PSO) to reformulate AFSA and improve its principal parameters. Simulation results show that under NUIC, compared with PSO and AFSA, the proposed algorithm has better performance with respect to convergence speed and convergence accuracy. Under NUIC, the average convergence times for 1000 simulation experiments completed with PSO, AFSA, and HAFSA are 0.4830 s, 0.4003 s and 0.3152 s respectively, and the average tracking time of the HAFSA algorithm is reduced by 34.74% and 21.26% compared with PSO and AFSA, respectively. The convergence times of the velocity inertia ω relative constant and linear decrement method decreased by 35.48% and 8.19%, the convergence time of the Visual relative constant mode decreased by 10.16%, and the convergence time of the Step relative constant mode decreased by 17.88%. The proposed GMPPT algorithm is simulated in MATLAB, and the algorithm tracks GMPP with excellent efficiency and fast speed. | Jianpo Li Pengwei Dong | 2019 | Global Energy Interconnection2019，2，4： | 7 |
| 6 | Dark matter direct search sensitivity of the PandaX-4T experiment显示文摘The Panda X-4T experiment, a 4-ton scale dark matter direct detection experiment, is being planned at the China Jinping Underground Laboratory. In this paper we present a simulation study of the expected background in this experiment. In a 2.8-ton fiducial mass and the signal region between 1-10 keV electron equivalent energy, the total electron recoil background is found to be 4.9 × 10^(-5) kg^(-1) d^(-1) keV^(-1). The nuclear recoil background in the same region is 2.8 × 10^(-7) kg^(-1) d^(-1) keV^(-1). With an exposure of 5.6 ton-years, the sensitivity of Panda X-4 T could reach a minimum spin-independent dark matter-nucleon cross section of 6 × 10^(-48) cm^2 at a dark matter mass of 40 Ge V/c^2. | HongGuang Zhang Abdusalam Abdukerim Wei Chen Xun Chen YunHua Chen XiangYi Cui BinBin Dong DeQing Fang ChangBo Fu Karl Giboni Franco Giuliani LinHui Gu XuYuan Guo ZhiFan Guo Ke Han ChangDa He ShengMing He Di Huang XingTao Huang Zhou Huang Peng Ji XiangDong Ji YongLin Ju ShaoLi Li Yao Li Heng Lin HuaXuan Liu JiangLai Liu YuGang Ma YaJun Mao KaiXiang Ni JinHua Ning XiangXiang Ren Fang Shi AnDi Tan AnQing Wang Cheng Wang HongWei Wang Meng Wang QiuHong Wang SiGuang Wang XiuLi Wang XuMing Wang Zhou Wang MengMeng Wu ShiYong Wu JingKai Xia MengJiao Xiao PengWei Xie BinBin Yan JiJun Yang Yong Yang ChunXu Yu JuMin Yuan JianFeng Yue Dan Zhang Tao Zhang Li Zhao QiBin Zheng JiFang Zhou Ning Zhou XiaoPeng Zhou | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，3： | 6 |
| 7 | Synthesis,biological activities and 3D-QSAR studies of(R)-2-phenyl-4,5-dihydrothiazole-4-carboxamide derivatives containing a sulfur ether moiety显示文摘A series of(R)-2-phenyl-4,5-dihydrothiazole-4-carboxamide derivatives containing a sulfur ether moiety were synthesized and characterized on the basis of NMR and elemental analysis(EA). The crystal structure of(R)-N-(2-methyl-1-(methylthio)propan-2-yl)-2-(4-nitrophenyl)-4,5-dihydrothiazole-4-carboxamide(13 d) was determined to show R configuration. The bioasssy results indicated that most title compounds displayed good and broad spectrum antifungal activities against several phytopathogenic fungi. The structure activity relationships were discussed. Based on the antifungal activity of title compounds against Phytophthora capsici, a CoMSIA calculation was performed to establish a 3 D-QSAR model, which revealed that electrostatic and hydrophobic fields were the two most significant factors for antifungal activity. According to the established 3D-QSAR model, structure optimization was carried out to find(R)-N-((R)-1-(methylthio)propan-2-yl)-2-(p-tolyl)-4,5-dihydrothiazole-4-carboxamide(15 h)with excellent activity against Phytophthora capsici, thus emerging as a new lead compound for novel antiphytopathogenic fungus agent development. | Jingbo Liu Fengyun Li Yuanhong Wang Haoxuan Zhang Jingyue Dong Pengwei Sun Yuxin Li Zhengming Li | 2019 | Chinese Chemical Letters2019，30，3： | 5 |
| 8 | Synthesis and Insecticidal Evaluation of Novel Anthranilic Diamides Derivatives Containing 4-Chlorine Substituted N-Pyridylpyrazole显示文摘To search for potent insecticides targeting at ryanodine receptors(RyRs),a series of novel anthranilic diamides analogs containing 4-chlorine N-pyridylpyrazole were designed and synthesized.Their insecticidal activities were evaluated and the preliminary struc ture-activity relationships(SARs)were discussed.The insecticidal results showed that some of the compounds(8a-8h,8m,8n)exhibited good larvicidal activities against oriental armyworm at 2.5 mg·L^(-1),and compound 8m possessed 60%insecticidal activityat 0.5 mg·L^(-1).For diamondback moth,8m exhibited better activity than Chlorantraniliprole at a hundred fold preference. | Huangong Li Yangyang Zhao Pengwei Sun Li Gao Yuxin Li Lixia Xiong Na Yang Sha Zhou Zhengming Li | 2021 | Chinese Journal of Chemistry2021，39，1： | 3 |
| 9 | The genome and gene editing system of sea barleygrass provide a novel platform for cereal domestication and stress tolerance studies显示文摘The tribe Triticeae provides important staple cereal crops and contains elite wild species with wide geneticdiversity and high tolerance to abiotic stresses. Sea barleygrass (Hordeum marinum Huds.), a wildTriticeae species, thrives in saline marshlands and is well known for its high tolerance to salinity and waterlogging. Here, a 3.82-Gb high-quality reference genome of sea barleygrass is assembled de novo, with 3.69Gb (96.8%) of its sequences anchored onto seven chromosomes. In total, 41 045 high-confidence (HC)genes are annotated by homology, de novo prediction, and transcriptome analysis. Phylogenetics, nonsynonymous/synonymous mutation ratios (Ka/Ks), and transcriptomic and functional analyses provide genetic evidence for the divergence in morphology and salt tolerance among sea barleygrass, barley, andwheat. The large variation in post-domestication genes (e.g. IPA1 and MOC1) may cause interspecies differences in plant morphology. The extremely high salt tolerance of sea barleygrass is mainly attributed tolow Na+ uptake and root-to-shoot translocation, which are mainly controlled by SOS1, HKT, and NHX transporters. Agrobacterium-mediated transformation and CRISPR/Cas9-mediated gene editing systems weredeveloped for sea barleygrass to promote its utilization for exploration and functional studies of hubgenes and for the genetic improvement of cereal crops. | Liuhui Kuang Qiufang Shen Liyang Chen Lingzhen Ye Tao Yan Zhong-Hua Chen Robbie Waugh Qi Li Lu Huang Shengguan Cai Liangbo Fu Pengwei Xing Kai Wang Jiari Shao Feibo Wu Lixi Jiang Dezhi Wu Guoping Zhang | 2022 | Plant Communications2022，3，5： | 2 |
| 10 | Polydopamine-modified collagen sponge scaffold as a novel dermal regeneration template with sustained release of platelet-rich plasma to accelerate skin repair:A one-step strategy显示文摘Although employed to release growth factors(GFs)for regenerative medicine,platelet-rich plasma(PRP)has been hindered by issues like burst effect.Based on collagen sponge scaffolds(CSSs)modified with polydopamine(pDA),a novel dermal regeneration template(DRT)was designed.However,whether it could efficiently deliver PRP and even foster wound healing remained unclear.In this work,after PRP was prepared and pDA-modified CSSs(pDA-CSSs)were fabricated,microscopic observation,GFs release assay and in-vitro biological evaluations of pDA-CSSs with PRP(pDA-CSS@PRP)were performed,followed by BALA-C/nu mice full-thickness skin defects implanted with pDA-CSS@PRP covered by grafted skins(termed as a One-step strategy).As a result,scanning electron microscope demonstrated more immobilized platelets on pDA-CSS′surface with GFs’controlled release via enzyme-linked immunosorbent assay,compared with CSSs.In line with enhanced in-vitro proliferation,adhesion and migration of keratinocytes&endothelial cells,pDA-CSS@PRP were histologically revealed to accelerate wound healing with less scar via rapid angiogenesis,arrangement of more mature collagen,guiding cells to spread,etc.In conclusion,pDA-CSSs have potential to serve as a novel DRT capable of delivering PRP,which may foster full-thickness skin defect healing by means of a One-step strategy. | Zijun Zheng Minxiong Li Pengwei Shi Yanbin Gao Jun Ma Yuchen Li Lei Huang Zhangfeifan Yang Lei Yang | 2021 | Bioactive Materials2021，6，8： | 2 |
| 11 | Fabricated temperature sensitive photocatalyst of PNIPAM@ZnO/C for controllable photocatalytic activity显示文摘PNIPAM@ZnO/C composite photocatalyst was prepared by cross-linking polymerization technology with N-isopropylacrylamide used as functional monomer, N,N'-methylenebis(acrylamide) used as crosslinking agent, ammonium persulfate used as initiator, and 3-(trimethoxysilyl) propyl methacrylate used as surface modification reagent. The morphology, structure, electrochemical and photocatalytic properties of as-prepared samples were characterized via the serial tests. The temperature-response performances of PNIPAM@ZnO/C were evaluated by the photocatalytic degradation of tetracycline(TC)under different temperatures. The results show that the synthesized composite photocatalysts possess the excellent and switchable photocatalytic activity. The photocatalytic degradation activity of PNIPAM@ZnO/C is suppressed above the lower critical solution temperature(LCST), and it is enhanced below the LCST. | Pengwei Huo Jinze Li Zhefei Ye Huiqin Wang Xinlin Liu Xiuying Li Yongsheng Yan | 2017 | Chinese Chemical Letters2017，28，12： | 2 |
| 12 | Corrigendum to“SARS-CoV-2 induced intestinal responses with a biomimetic human gut-on-chip”[Sci.Bull.(2021)66(8)783-793]显示文摘The authors would like to correct Fig.2g.An annotation error was introduced in the preparation of this figure for publication.The authors declare that this correction does not change the results or conclusions of this paper.The authors sincerely apologize for having this error in the article,and apologize for any inconvenience caused. | Yaqiong Guo Ronghua Luo Yaqing Wang Pengwei Deng Tianzhang Song Min Zhang Peng Wang Xu Zhang Kangli Cui Tingting Tao Zhongyu Li Wenwen Chen Yongtang Zheng Jianhua Qin | 2021 | Science Bulletin2021，66，20： | 2 |
| 13 | Synthesis and characterization of PEG-PCL-PEG thermosensitive hydrogel显示文摘 | ChangYang Gong Shuai Shi PengWei Dong Bing Kan MaLing Gou XianHuo Wang XingYi Li Feng Luo Xia Zhao YuQuan Wei ZhiYong Qian | 2008 | International Journal of Pharmaceutics2008，，1： | 1 |
| 14 | Droplet Manipulation and Crystallization Regulation in Inkjet-Printed Perovskite Film Formation显示文摘Emerging organic–inorganic metal halide perovskite materials have become the focus of the optoelectronics research community owing to their excellent photoelectric properties.Nevertheless,challenges still exist for transferring the lab-made devices to largearea industrial modules.Inkjet printing(IJP)technology provides a promising way to fill the gap because of its precise droplet control and uniform large-scale deposition functions.Hence,an in-depth understanding of inkjet-printed perovskite films in terms of droplet manipulation and crystallization regulation is critical for upscaling the perovskite devices to commercial usage.In this review,we give an overview of inkjet-printed high-quality perovskite films and provide guidelines on inkjet-printing large-scale highperformance perovskite devices.First,we analyze theories of droplet formation and perovskite nucleation/crystallization dynamics and then focus on summarizing the perovskite film-formation strategies via IJP,in the aspects of ink engineering,the printing process,and posttreatment.Furthermore,we review the recent advances of inkjet-printed perovskite films on optoelectronic devices,such as perovskite solar cells,perovskite light-emitting diodes,and perovskite photodetectors.Finally,we highlight the“Trilogy Strategies,”including ink engineering,printing process,and posttreatment for printing high-quality perovskite films. | Yajie Cheng Hangjuan Wu Junjie Ma Pengwei Li Zhenkun Gu Shuangquan Zang Liyuan Han Yiqiang Zhang Yanlin Song | 2022 | CCS Chemistry2022，4，5： | 1 |
| 15 | Envelope protection for aircraft encountering upset condition based on dynamic envelope enlargement显示文摘Upset condition encountered by an aircraft in flight could pose great threat to flight safety, which is of chief importance in civil aviation. The causal factors have the nonlinear and multiple characteristics, and as a result the conventional envelope protection system cannot successfully do with the condition. So dynamic envelope based on differential manifold theory, which can take more coupling factors into account, is proposed as a basis to design a novel envelope protection system. Then the relationship between the dynamic envelope and the control coefficient or pilot command is obtained, and the result shows that the dynamic envelope can be enlarged with the change of control coefficient. Furthermore, quantification of flight security is realized via defining relative distance between flight state and dynamic envelope, which can detect whether there is a risk for an aircraft in flight. Finally, an envelope protection system based on dynamic envelope enlargement is proposed on the basis. NASA's Generic Transport Model encountering hazard gust wind in climbing phase is taken as an example to verify the system's feasibility. The result shows that the system can give a better operation encountering upset condition and to a certain extent reduce the number of accidents or incidents. | Wuji ZHENG Yinghui LI Dengcheng ZHANG Chi ZHOU Pengwei WU | 2018 | Chinese Journal of Aeronautics2018，31，7： | 1 |
| 16 | Maximum power point tracking of PV system under partial shading conditions through flower pollination algorithm显示文摘For maximum utilization of solar energy,photovoltaic(PV)power systems should be operated at the maximum power point(MPP)which can be achieved using maximum power point tracking(MPPT)methods.However,the occurrence of multi-peak on P-V curve of a PV array due to the changing environmental conditions such as being partially shaded increases the complexity of the tracking process.The global MPP cannot always be achieved by the conventional MPPT methods.Therefore a novel MPPT method for PV systems using flower pollination(FP)algorithm is proposed in this paper and the Levy flight is used to improve the convergence of FP algorithm.MPPT model of the PV system is established in MATLAB to verify the effectiveness of the proposed method,and the proposed method is compared with two well established MPPT methods.The simulation results indicate that the proposed MPPT method can quickly track the changes in external environment and effectively handle the partially shaded condition. | Liqun Shang Weiwei Zhu Pengwei Li Hangchen Guo | 2018 | Protection and Control of Modern Power Systems2018，3，1： | 1 |
| 17 | Preparation and characterization of Cobalt Sulfophthalocyanine/TiO 2 /fly-ash cenospheres photocatalyst and study on degradation activity under visible light显示文摘 | Pengwei Huo Yongsheng Yan Songtian Li Huaming Li Weihong Huang | 2009 | Applied Surface Science2009，，15： | 1 |
| 18 | Preparation of Poly-o-Phenylenediamine/TiOJFly-Ash Cenospheres and Its Photo-Degradation Property on Antibiotics 显示文摘 | Huo Pengwei Yan Yongsheng Li Songtian | 2010 | Appl SurfSci2010，256，11： | 1 |
| 19 | Solar-tracking methodology based on refraction-polarization in Snell's window for underwater navigation显示文摘Underwater navigation system is an indispensable part for autonomous underwater vehicles.Due to the indiscernibility of satellite signal,however,the underwater navigation problem is quite challenging,and a satellite-free navigation scheme should be looked for.Polarization navigation,inspired by insects’capability of autonomous homing and foraging,is an alternative solution to satellite navigation with great application potential.Underwater polarization provides an indirect sun compass to animals for orientation determination.However,it is difficult to apply terrestrial solar-tracking methodologies in underwater situations due to the refraction of polarized skylight at the air–water interface.To resolve this issue,an underwater solar-tracking algorithm is developed based on the underwater refraction-polarization pattern inside the Snell’s window.By employing Snell’s law and Fresnel refraction formula to decouple the refractive ray bending and polarization deflection,the celestial polarization pattern is obtained based on underwater measurement.To further improve the accuracy,the degree of polarization is employed as a weight factor for E-vector.A long-lasting underwater experiment was conducted to validate the effectiveness of the proposed approach,and the results showed the root-mean-square errors of solar zenith and azimuth employing this algorithm were 0.3°and 1.3°,respectively.Our experimental results show that the refraction-polarization pattern inside the Snell’s window exhibits immense potential to improve the solar-tracking accuracy for underwater navigation. | Pengwei HU Jian YANG Lei GUO Xiang YU Wenshuo LI | 2022 | Chinese Journal of Aeronautics2022，35，3： | 1 |
| 20 | Activation of three natural product biosynthetic gene clusters from Streptomyces lavendulae CGMCC 4.1386 by a reporter-guided strategy显示文摘Along with the fast developing of DNA sequencing technology,a great number of natural product biosynthetic gene clusters have been discovered by bioinformatic analysis,which demands novel high-throughput genome mining methods to obtain the diverse compounds dictated by those gene clusters.In this work,a method based on the reporter gene xylE was established to screen for the activation conditions of thirteen different gene clusters from Streptomyces lavendulae CGMCC 4.1386.In this reporter-guided method,the key structure gene was replaced by a xylE-kanaR cassette with the xylE gene being controlled by the transcription and translation machinery of the key structure gene.It not only facilitated the screening of activation conditions,but also provided the null mutants of specific natural product gene clusters as controls to link those clusters with their products conveniently.The potential activation conditions of eleven gene clusters from S.lavendulae CGMCC 4.1386 were obtained.In addition,activation of three of the eleven gene clusters was confirmed and their products were identified. | Pengwei Li Zhengyan Guo Wei Tang Yihua Chen | 2018 | Synthetic and Systems Biotechnology2018，3，4： | 1 |
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