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| 1 | Multiple genetic alterations and behavior of cellular biology in gastric cancer and other gastric mucosal lesions:H.pylori infection,histological types and staging显示文摘AIM To investigate the expression of multiplegenes and the behavior of cellular biology ingastric cancer(GC)and other gastric mucosallesions and their relations to Helicobacter pylori(H.pylori)infection,tumor staging andhistological subtypes.METHODS Three hundred and twenty-sevenspecimens of gastric mucosa obtained viaendoscopy or surgical resection,and ABCimmunohistochemical staining were used todetect the expression of p53,p16,Bcl-2 andCOX-2 proteins.H.pylori was determined byrapid urea test combined with pathologicalstaining or14C urea breath test.Cellular image analysis was performed in 66 patients withintestinal metaplasia(IM)and/or dysplasia(Dys).In 30 of them,both cancer and theparacancerous tissues were obtained at the timeof surgery.Histological pattern,tumor staging,lymph node metastasis,grading ofdifferentiation and other clinical data werestudied in the medical records.RESULTS p16 expression of IM or Dys wassignificantly lower in positive H.pylori chronicatrophic gastritis(CAG)than those withnegative H.pylori(CAG:54.8% vs 88.0%,IM:34.4% vs 69.6%,Dys:23.8% vs 53.6%,allP<0.05),Bcl-2 or COX-2 expression of IM orDys in positive H.pylori cases was significantlyhigher than that without H.pylori(Bcl-2:68.8%vs23.9%,90.5% vs 60.7%;COX-2:50.0% vs10.8%,61.8% vs 17.8%;all P<0.05).Themean number of most parameters of cellularimage analysis in positive H.pylori group wassignificantly higher than that in negative H.pylori group(Ellipser:53±14,40±12μm,Area1:748±572,302±202 μm2,Area2:3050±1661,1681±1990 μm2,all P<0.05;Ellipseb:79±23,58±15 μm,Ratio1:22%±5%,13%±4%,Ratio2:79%±17%,53%±20%,all P<0.01).There was significant correlation between Bcl-2and histologic pattern of gastric carcinoma,andbetween COX-2 and tumor staging or lymph nodemetastasis(Bcl-2:75.0% vs 16.7%;COX-2:76.0% vs 20.0%,79.2% vs 16.7%;allP<0.05).CONCLUSION p1l6, Bcl-2, and COX-2 but not p53 gene may play a role in the early genesis/ progression of gastric carcinoma and are associated with H. pylori infection. p53 gene is relatively late event in gastric tumorigenesis and mainly relates to its progression. There is more cellular-biological behavior of malignant tumor in gastric mucosal lesions with H. pylori infection. Aberrant Bcl-2 protein expression appears to be preferentially associated with the intestinal type cancer. COX-2 seems to be related to tumor staging and lymph node metastasis. | Heng Jun Gao Lian Zhen Yu Jian Feng Bai Yan Shen Peng Gu Sun Han Lin Zhao Kun Miu Xiu Zhen Lü Xiao Yong Zhang Zhi Quan Zhao | 2000 | World Journal of Gastroenterology2000，6，6： | 52 |
| 2 | Apoptosis and Proinflammatory Cytokine Responses of Primary Mouse Microglia and Astrocytes Induced by Human H1N1 and Avian H5N1 Influenza Viruses显示文摘Patients with an influenza virus infection can be complicated by acute encephalopathy and encephalitis. To investigate the immune reactions involved in the neurocomplication,mouse microglia and astrocytes were isolated,infected with human H1N1 and avian H5N1 influenza viruses,and examined for their immune responses. We observed homogeneously distributed viral receptors,sialic acid (SA)-α2,3-Galactose (Gal) and SA-α2,6-Gal,on microglia and astrocytes. Both viruses were replicative and productive in microglia and astrocytes. Virus-induced apoptosis and cytopathy in infected cells were observed at 24 h post-infection (p.i.). Expression of IL-1β,IL-6 and TNF-α mRNA examined at 6 h and 24 h p.i. was up-regulated,and their expression levels were considerably higher in H5N1 infection. The amounts of secreted proinflammatory IL-1β,IL-6 and TNF-α at 6 h and 24 h p.i. were also induced,with greater induction by H5N1 infection. This study is the first demonstration that both human H1N1 and avian H5N1 influenza viruses can infect mouse microglia and astrocytes and induce apoptosis,cytopathy,and proinflammatory cytokine production in them in vitro. Our results suggest that the direct cellular damage and the consequences of immunopathological injury in the CNS contribute to the influenza viral pathogenesis. | Gefei Wang Juan Zhang Weizhong Li Yun Su Yuanli Gao Heng Zhang Guimei Lin Xiaoyang Jiao Kangsheng Li | 2008 | Cellular & Molecular Immunology2008，5，2： | 28 |
| 3 | Comparison of the effect and safety of Kuntai capsule and hormone replacement therapy in patients with perimenopausal syndrome:a systematic review and Meta-analysis显示文摘OBJECTIVE: To assess the effectiveness and safety of Kuntai capsule and hormone replacement therapy in treatment of perimenopausal syndrome.METHODS: Articles were retrieved from the databases Cochrane Database of Systematic Reviews,Pub Med, Chinese National Knowledge Infrastructure, China Science and Technology Journal Database, and Wanfang Database. Only randomized controlled trials were included; 15 trials involving1243 patients were identified from January 2005 to April 2015. A systemic review and Meta-analysis of publications was performed. The review was limit-ed to randomized controlled trials that compared Kuntai capsule and hormone replacement therapy to treat perimenopausal syndrome for at least 3months. The primary outcome assessed was the treatment efficacy at 3 months, including effective rate of Kupperman menopausal scores, Kupperman menopausal scores, and blood estradiol(E2) or blood follicle stimulating hormone(FSH) levels.Other outcomes assessed were safety or adverse events, such as gastrointestinal complaints, breast distending pain, or vaginal bleeding.RESULTS: Kupperman menopausal scores showed no significant difference in effective rate [odds ratio(OR): 1.05, 95% confidence intervals(CI): 0.71 to1.55] and changes in FSH level [mean difference(MD): 2.14, 95% CI:-2.36 to 6.65]. There was a significant statistical difference in Kupperman menopausal scores(MD:-1.14, 95% CI:-2.03 to-0.25)and changes in E2level(MD:-16.41, 95% CI:-18.83to-13.69). There were fewer adverse events in the Kuntai capsule group than in the hormone replacement therapy group(OR: = 0.35, 95% CI: 0.25 to0.48, P < 0.01).CONCLUSION: Compared with hormone replacement therapy, Kuntai capsule can improve perimenopausal symptoms and blood E2 levels, and reduce the incidence of adverse events. | Du Xiaoqin Xu Lin Wang Lijun Heng Mingli Bu Huaien Hao Yu Tian Jinhui | 2017 | Journal of Traditional Chinese Medicine2017，37，3： | 28 |
| 4 | The antitumor effect of tanshinone IIA on antiproliferation and decreasing VEGF/VEGFR2 expression on the human non-small cell lung cancer A549 cell line显示文摘The effects of tanshinone IIA on the proliferation of the human non-small cell lung cancer cell line A549 and its possible mechanism on the VEGF/VEGFR signal pathway were investigated.The exploration of the interaction between tanshinone IIA and its target proteins provides a feasible platform for studying the anticancer mechanism of active components of herbs.The CCK-8 assay was used to evaluate the proliferative activity of A549 cells treated with tanshinone IIA(2.5 80 μmol/L) for 24,48 and 72 h,respectively.Flow cytometry was used for the detection of cell apoptosis and cell cycle perturbation.VEGF and VEGFR2 expression were studied by Western blotting.The binding mode of tanshinone IIA within thecrystal structure of the VEGFR2 protein was evaluated with molecular docking analysis by use of the CDOCKER algorithm in Discovery Studio 2.1.The CCK-8 results showed that tanshinone IIA can significantly inhibit A549 cell proliferation in a dose-and time-dependent manner.Flow cytometry results showed that the apoptosis rate of tested group was higher than the vehicle control,and tanshinone IIAtreated cells accumulated at the S phase,which was higher than the vehicle control.Furthermore,the expression of VEGF and VEGFR2 was decreased in Western blot.Finally,molecular docking analysis revealed that tanshinone IIA could be stably docked into the kinase domain of VEGFR2 protein with its unique modes to form H-bonds with Cys917 and π–π stacking interactions with Val848.In conclusion,tanshinone IIA may suppress A549 proliferation,induce apoptosis and cell cycle arrest at the S phase.This drug may suppress angiogenesis by targeting the protein kinase domains of VEGF/VEGFR2. | Jun Xie Jiahui Liu Heng Liu Shihui Liang Meigui Lin Yueyu Gu Taoli Liu Dongmei Wang Hui Gee Sui-lin Mo | 2015 | Acta Pharmaceutica Sinica B2015，5，6： | 26 |
| 5 | Nat Chem Biol:利用CRISPR-Cas9激活细菌中沉默的基因簇,有望发现新的药物显示文摘为了抵抗疾病,很多医药库中的武器是从细菌当中获得的。如今,利用CRISPR-Cas9基因编辑技术。研究人员揭示出沉默基因中隐藏着的更多潜在的宝藏。作为一类常见的细菌。链霉菌被用来产生很多作为抗生素、抗癌试剂和其他药物的化合物。在一项新的研究中。来自美国伊利诺伊大学和新加坡科技研究局的研究人员利用CRISPR-Cas9技术激活链霉菌中不表达的或者说沉默的基因簇。 | Mingzi M Zhang, Wan Lin Yeo, Ee Lui Ang, Huimin Zhao Fong Tian Wong, Elena Heng Yajie Wang, Shangwen Luo, Ryan E Cobb, Behnam Enghiad, Huimin Zhao Yee Hwee Lim | 2017 | 现代生物医学进展2017，17，17： | 12 |
| 6 | Efficacy and safety of secukinumab in Chinese patients with moderate-to-severe plaque psoriasis: a real-life cohort study显示文摘Background:There have been few real-life dose-comparing studies on the efficacy and safety of secukinumab in Chinese patients with plaque psoriasis.We conducted a real-life cohort study to investigate the efficacy and safety of secukinumab 150 and 300 mg in Chinese patients with moderate-to-severe plaque psoriasis.Methods:A total of 106 patients with moderate-to-severe plaque psoriasis were included in this study.Patients received either secukinumab 150 mg or secukinumab 300 mg according to patients’weights and severity of psoriasis.The treatment continued for at least 24 weeks.The efficacy was evaluated by improvement in the psoriasis area and severity index(PASI)scores.The safety was also analyzed.Results:Fifty-nine patients(55.7%)were treated with secukinumab 300 mg and 47 patients(44.3%)were treated with secukinumab 150 mg.After 12-week treatment,PASI75/90/100 responses were achieved in 100%,97.8%,and 95.7%of patients,respectively,in secukinumab 150 mg group,and the efficacy was maintained to week 24.In secukinumab 300 mg group,PASI75/90/100 responses were achieved in 93.2%,81.4%,and 76.3%of patients,respectively,at week 12.In this group,PASI75/90/100 responses reached 91.5%,86.4%,and 79.9%,respectively,at week 24.Biologic-experienced patients had lower responses than biologic-naïve patients.Secukinumab 150 and 300 mg were well tolerated.Five patients discontinued treatment due to poor response,adverse event,or economic reasons.Conclusions:This real-life study demonstrated that high PASI 90 and PASI 100 responses were achieved in Chinese psoriasis patients receiving secukinumab 150 or 300 mg.Biologic-naïve was associated with better clinical efficacy. | Yan Zhao Lin Cai Xiao-Yang Liu Heng Zhang Jian-Zhong Zhang | 2021 | Chinese Medical Journal2021，，11： | 10 |
| 7 | Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45显示文摘Objective:Side population(SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer.Many kinds of cell lines and tissues have demonstrated the presence of SP cells,including several gastric cancer cell lines.This study is aimed to identify the cancer stem-like cells in the SP of gastric cancer cell line MKN-45.Methods:We used fluorescence activated cell sorting(FACS) to sort SP cells in the human gastric carcinoma cell line MKN-45(cells labeled with Hoechst 33342) and then characterized the cancer stem-like properties of SP cells.Results:This study found that the SP cells had higher clone formation efficiency than major population(MP) cells.Five stemness-related gene expression profiles,including OCT-4,SOX-2,NANOG,CD44,and adenosine triphosphate(ATP)-binding cassette transporters gene ABCG2,were tested in SP and MP cells using quantitative real-time reverse transcription polymerase chain reaction(RT-PCR).Western blot was used to show the difference of protein expression between SP and MP cells.Both results show that there was significantly higher protein expression in SP cells than in MP cells.When inoculated into non-obese diabetic/severe combined immunodeficiency(NOD/SCID) mice,SP cells show higher tumorigenesis tendency than MP cells.Conclusions:These results indicate that SP cells possess cancer stem cell properties and prove that SP cells from MKN-45 are gastric cancer stem-like cells. | Hai-hong ZHANG Ai-zhen CA Xue-ming WEI Li DING Feng-zhi LI Ai-ming ZHENG Da-jiang DAI Rong-rong HUANG Hou-jun CAO Hai-yang ZHOU Jian-mei WANG Xue-jing WANG Wei SHI Heng ZHU Xiao-ying YUAN Lin CHEN | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，3： | 9 |
| 8 | Synthesis, isolation and characterization of methyl levulinate from cellulose catalyzed by extremely low concentration acid显示文摘A direct synthesis of methyl levulinate from cellulose alcoholysis in methanol medium under mild condition(180 210 C)catalyzed by extremely low concentration sulfuric acid(0.01 mol/L)and the product isolation were developed in this study.Effects of different process variables towards the catalytic performance were performed as a function of reaction time.The results indicated that sulfuric acid concentration,temperature and initial cellulose concentration had significant effects on the synthesis of methyl levulinate.An optimized yield of around 50%was achieved at 210 C for 120 min with sulfuric acid concentration of 0.01 mol/L and initial cellulose concentration below 100 g/L.The resulting product mixture was isolated by a distillation technique that combines an atmospheric distillation with a vacuum distillation where n-dodecane was added to help distill the heavy fraction.The light fraction including mainly methanol could be reused as the reaction medium without any substantial change in the yield of methyl levulinate.The chemical composition and structural of lower heavy fraction were characterized by GC/MS,FTIR,1H-NMR and13C-NMR techniques.Methyl levulinate was found to be a major ingredient of lower heavy fraction with the content over 96%.This pathway is efficient,environmentally benign and economical for the production of pure levulinate esters from cellulose. | Hui Li Lincai Peng Lu Lin Keli Chen Heng Zhang | 2013 | Journal of Energy Chemistry2013，22，6： | 9 |
| 9 | 雷帕霉素对小鼠百草枯中毒导致肺纤维化的治疗作用(英文)显示文摘目的:评估雷帕霉素(RAPA)对小鼠百草枯(PQ)中毒导致肺纤维化的治疗作用,并初步探讨其作用机制。创新点:首次在小鼠PQ中毒的模型中证明RAPA可明显抑制肺纤维化,且此作用与转化生长因子-β1(TGF-β1)的抑制相关。方法:将C57BL/6J雄鼠分为对照组(腹腔注射生理盐水)和实验组(腹腔注射10 mg/kg PQ)。实验组根据采用的治疗不同,可分为以下四组:PQ组、PQ+RAPA组、PQ+MP(甲强龙)组和PQ+MP+RAPA组。用苏木精-伊红染色法(H&E)和马松(Masson)三色染色法观察肺组织病理结构变化,用酶联免疫吸附测定(ELISA)试剂盒检测肺组织中的羟脯氨酸(HYP)含量,用免疫组化和免疫印迹的方法检测TGF-β1和α-平滑肌肌动蛋白(α-SMA)的表达水平。结论:本实验中小鼠的肺组织病理染色结果显示,RAPA治疗能缓解肺纤维化导致的病理改变(图3)。ELISA实验结果显示,RAPA治疗28天后可显著降低肺组织中HYP的含量(图4)。免疫组化和免疫印迹实验结果显示,RAPA治疗能明显下调肺组织中TGF-β1和α-SMA的高表达(图5和6)。综上所述,RAPA在治疗PQ中毒导致的肺纤维化中有重要价值。 | Xue SHAO Meng LI Chong LUO Ying-ying WANG Ying-ying LU Shi FENG Heng LI Xia-bing LANG Yu-cheng WANG Chuan LIN Xiu-jin SHEN Qin ZHOU Hong JIANG Jiang-hua CHEN | 2015 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2015，16，1： | 7 |
| 10 | Immature CD4+ dendritic cells conditioned with donor kidney antigen prolong renal allograft survival in rats显示文摘 | WANG Tao XU Lin LI Heng HUANG Zheng-yu ZHANG Sheng-ping MIAO Bin NA Ning | 2012 | Chinese Medical Journal2012，，14： | 6 |
| 11 | Lung-protective Ventilation in Patients with Brain Injury: A Multicenter Cross-sectional Study and Questionnaire Survey in China显示文摘 | Xu-Ying Luo Ying-Hong Hu Xiang-Yuan Cao Yan Kang Li-Ping Liu Shou-Hong Wang Rong-Guo Yu Xiang-You Yu Xia Zhang Bao-Shan Li Zeng-Xiang Ma Yi-Bing Weng Heng Zhang De-Chang Chen Wei Chen Wen-Jin Chen Xiu-Mei Chen Bin Du Mei-Li Duan Jin Hu Yun-Feng Hoang Gui-Jun Jia Li-Hong Li Yu-Min Liang Bing-Yu Qin Xian-Dong Wang Jian Xiong Li-Mei Yan Zheng-Ping Yang Chen-Ming Dong Dong-Xin Wang Qing-Yuan Zhan Shuang-Lin FU Lin Zhao Qi-Bing Huang Ying-Guang Xie Xiao-Bo Huang Guo-Bin Zhang Wang-Bin Xu Yuan Xu YaLing Liu He-Ling Zhao Rong-Qing Sun Ming Sun Qing-Hong Cheng Xin Qu Xiao-Feng Yang Ming Xu Zhong-Hua Shi Han Chen Xuan He Yan-Lin Yang Guang-Qiang Chen Xiu-Mei Sun Jian-Xin Zhou | 2016 | Chinese Medical Journal2016，，14： | 6 |
| 12 | Dark matter direct search sensitivity of the PandaX-4T experiment显示文摘The Panda X-4T experiment, a 4-ton scale dark matter direct detection experiment, is being planned at the China Jinping Underground Laboratory. In this paper we present a simulation study of the expected background in this experiment. In a 2.8-ton fiducial mass and the signal region between 1-10 keV electron equivalent energy, the total electron recoil background is found to be 4.9 × 10^(-5) kg^(-1) d^(-1) keV^(-1). The nuclear recoil background in the same region is 2.8 × 10^(-7) kg^(-1) d^(-1) keV^(-1). With an exposure of 5.6 ton-years, the sensitivity of Panda X-4 T could reach a minimum spin-independent dark matter-nucleon cross section of 6 × 10^(-48) cm^2 at a dark matter mass of 40 Ge V/c^2. | HongGuang Zhang Abdusalam Abdukerim Wei Chen Xun Chen YunHua Chen XiangYi Cui BinBin Dong DeQing Fang ChangBo Fu Karl Giboni Franco Giuliani LinHui Gu XuYuan Guo ZhiFan Guo Ke Han ChangDa He ShengMing He Di Huang XingTao Huang Zhou Huang Peng Ji XiangDong Ji YongLin Ju ShaoLi Li Yao Li Heng Lin HuaXuan Liu JiangLai Liu YuGang Ma YaJun Mao KaiXiang Ni JinHua Ning XiangXiang Ren Fang Shi AnDi Tan AnQing Wang Cheng Wang HongWei Wang Meng Wang QiuHong Wang SiGuang Wang XiuLi Wang XuMing Wang Zhou Wang MengMeng Wu ShiYong Wu JingKai Xia MengJiao Xiao PengWei Xie BinBin Yan JiJun Yang Yong Yang ChunXu Yu JuMin Yuan JianFeng Yue Dan Zhang Tao Zhang Li Zhao QiBin Zheng JiFang Zhou Ning Zhou XiaoPeng Zhou | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，3： | 6 |
| 13 | Resveratrol inhibits collagen Ⅰ synthesis by suppressing IGF-1R activation in intestinal fibroblasts显示文摘AIM:To investigate whether resveratrol(3,4,5-trihydroxy-trans-stilbene)inhibits collagenⅠsynthesis induced by insulin growth factor-1(IGF-1)in intestinal fibroblasts,and to explore the possible molecular mechanisms.METHODS:Male Sprague-Dawley rats were randomly divided into two groups:a control group and a2,4,6-trinitrobenzenesulfonic acid(TNBS)-induced colitis group.After 21 d of TNBS administration,the degree of inflammation and fibrosis in colon was measured by HE staining and Masson’s trichrome staining.Western blotting was used to examine collagenⅠ,IGF-1 and silent information regulator 1(SIRT1)protein expression in colitis tissues.Western blotting and quantitative realtime polymerase chain reaction were used to characterize collagenⅠprotein and col1a2 mRNA expression in mouse intestinal fibroblasts and CCD-18Co cells treated with IGF-1.A MEK inhibitor(U0126)was used to determine whether IGF-1-induced collagenⅠexpression was mediated by extracellular signal-regulated kinase 1/2(ERK1/2)-dependent mechanism.Effects of resveratrol on collagenⅠprotein level,insulin growth factor-1receptor(IGF-1R)and ERK1/2 phosphorylation levels were also examined after IGF-1 treatment in fibroblasts.To evaluate whether SIRT1 was necessary for the anti-fibrosis effect of resveratrol,cells were transfected with SIRT1-specific small interfering RNAs,wildtype SIRT1,and deacetylase-inactive mutant SIRT1.RESULTS:CollagenⅠand IGF-1 expression was increased,and SIRT1 expression was decreased(0.67±0.04 vs 1.05±0.07,P<0.001)in TNBS-induced colitis compared with the control group.In vitro,IGF-1 could induce collagenⅠexpression,mainly through the ERK1/2 signal pathway.Resveratrol reduced basal and IGF-1-induced collagenⅠ?gene and protein expression in intestinal fibroblasts.Overexpression of wild-type SIRT1,not deacetylase-inactive mutant SIRT1,decreased expression of collagenⅠinduced by IGF-1.Moreover,silencing SIRT1 restored collagen?Ⅰ?expression in fibroblasts challenged with resveratrol.However,disruption of SIRT1 did not influence the anti-fibrotic effects of resveratrol and IGF-1-induced collagenⅠexpression.Further analysis revealed that resveratrol significantly decreased phosphorylation of IGF-1R and its downstream signaling molecules by inhibiting IGF-1 binding to its receptor.CONCLUSION:Our data suggest that resveratrol effectively inhibits collagenⅠsynthesis in IGF-1-stimulated fibroblasts,partly by inhibiting IGF-1R activation,and SIRT1 is also responsible for the process. | Ping Li Mei-Lan Liang Ying Zhu Yao-Yao Gong Yun Wang Ding Heng Lin Lin | 2014 | World Journal of Gastroenterology2014，20，16： | 6 |
| 14 | Age and menopausal status are important factors influencing the serum human epididymis secretory protein 4 level:a prospective cross-sectional study in healthy Chinese people显示文摘Background::Human epididymis secretory protein 4(HE4)is a new ovarian cancer biomarker.The factors influencing HE4 levels are not clear,and the reference data in China are limited.Here,we aim to evaluate the effects of menopause and age on HE4 levels and to provide a possible reference value for HE4 in healthy Chinese people.Methods::A total of 2493 healthy females aged 40 years or older were recruited from March 2013 to March 2017 with the cooperation of four medical institutions across Beijing,China.The serum levels of HE4 and cancer antigen 125(CA125)were measured by enzyme-linked immunosorbent assay.The Wilcoxon rank-sum test of variance and a stratified analysis were used to analyze the relationships among age,menopausal status,and levels of HE4 or CA125.Confidence intervals(5%-95%)were determined for reference ranges in different populations.Results::There was a statistically significant difference in median HE4 levels between the post-menopausal(n=2168)and premenopausal groups(n=325)(36.46 vs.24.04 pmol/L,Z=-14.41,P<0.001).HE4 increased significantly with age in the post-menopausal groups(H=408.18,P<0.001)but not in the pre-menopausal subjects(Z=-0.43,P=0.67).The upper 95th percentile of HE4 levels were 44.63 pmol/L for pre-menopausal women,78.17 pmol/L for post-menopausal women,and 73.3 pmol/L for all women.In the post-menopausal population,the HE4 reference ranges were 13.15 to 47.31,14.31 to 58.04,17.06 to 73.51,24.50 to 115.25,and 35.71 to 212.37 pmol/L for different age groups from forty divided by decade.The CA125 level was affected mainly by menopausal status and not age.Conclusions::Menopausal status and age were both important factors influencing the level of HE4,and age affected HE4 levels mainly in post-menopausal women.The HE4 level was higher in the post-menopausal population than in the pre-menopausal population and increased with age. | Hong-Yan Cheng Lin Zeng Xue Ye Rui-Qiong M a Zhi-Jian Tang Hong-Ling Chu Yi-M ing Zhao Li-Rong Zhu Yu-Nong Gao Xiao-Hong Chang Heng Cui | 2020 | Chinese Medical Journal2020，，11： | 6 |
| 15 | Curcumin inhibits the replication of enterovirus 71 in vitro显示文摘Human enterovirus 71(EV71)is the main causative pathogen of hand,foot,and mouth disease(HFMD)in children.The epidemic of HFMD has been a public health problem in Asia-Pacific region for decades,and no vaccine and effective antiviral medicine are available.Curcumin has been used as a traditional medicine for centuries to treat a diversity of disorders including viral infections.In this study,we demonstrated that curcumin showed potent antiviral effect again EV71.In Vero cells infected with EV71,the addition of curcumin significantly suppressed the synthesis of viral RNA,the expression of viral protein,and the overall production of viral progeny.Similar with the previous reports,curcumin reduced the production of ROS induced by viral infection.However,the antioxidant property of curcumin did not contribute to its antiviral activity,since N-acetyl-L-cysteine,the potent antioxidant failed to suppress viral replication.This study also showed that extracellular signal-regulated kinase(ERK)was activated by either viral infection or curcumin treatment,but the activated ERK did not interfere with the antiviral effect of curcumin,indicating ERK is not involved in the antiviral mechanism of curcumin.Unlike the previous reports that curcumin inhibited protein degradation through ubiquitin–proteasome system(UPS),we found that curcumin had no impact on UPS in control cells.However,curcumin did reduce the activity of proteasomes which was increased by viral infection.In addition,the accumulation of the short-lived proteins,p53 and p21,was increased by the treatment of curcumin in EV71-infected cells.We further probed the antiviral mechanism of curcumin by examining the expression of GBF1 and PI4KB,both of which are required for the formation of viral replication complex.We found that curcumin significantly reduced the level of both proteins.Moreover,the decreased expression of either GBF1 or PI4KB by the application of siRNAs was sufficient to suppress viral replication.We also demonstrated that curcumin showed anti-apoptotic activity at the early stage of viral infection.The results of this study provide solid evidence that curcumin has potent anti-EV71 activity.Whether or not the down-regulated GBF1 and PI4KB by curcumin contribute to its antiviral effect needs further studies. | Ying Qin Lexun Lin Yang Chen Shuo Wu Xiaoning Si Heng Wu Xia Zhai Yan Wang Lei Tong Bo Pan Xiaoyan Zhong Tianying Wang Wenran Zhao Zhaohua Zhong | 2014 | Acta Pharmaceutica Sinica B2014，4，4： | 5 |
| 16 | Surface modification of LiNi_(1/3)Co_(1/3)Mn_(1/3)O_2 with Cr_2O_3 for lithium ion batteries显示文摘Cr 2 O 3-coated LiNi 1/3 Co 1/3 Mn 1/3 O 2 cathode materials were synthesized by a novel method. The structure and electrochemical properties of prepared cathode materials were measured using X-ray diffraction (XRD), scanning electron microscopy (SEM), charge-discharge tests, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The measured results indicate that surface coating with 1.0 wt% Cr 2 O 3 does not affect the LiNi 1/3 Co 1/3 Mn 1/3 O 2 crystal structure (α-NaFeO 2 ) of the cathode material compared to the pristine material, the surfaces of LiNi 1/3 Co 1/3 Mn 1/3 O 2 samples are covered with Cr 2 O 3 well, and the LiNi 1/3 Co 1/3 Mn 1/3 O 2 material coated with Cr 2 O 3 has better electrochemical performance under a high cutoff voltage of 4.5 V. Moreover, at room temperature, the initial discharging capacity of LiNi 1/3 Co 1/3 Mn 1/3 O 2 material coated with 1.0 wt.% Cr 2 O 3 at 0.5C reaches 169 mAh·g 1 and the capacity retention is 83.1% after 30 cycles, while that of the bare LiNi 1/3 Co 1/3 Mn 1/3 O 2 is only 160.8 mAh·g 1 and 72.5%. Finally, the coated samples are found to display the improved electrochemical performance, which is mainly attributed to the suppression of the charge-transfer resistance at the interface between the cathode and the electrolyte. | Li, Xiaowei Lin, Yingbin Liin, Ying Lai, Heng Huang, Zhigao | 2012 | Rare Metals2012，31，2： | 4 |
| 17 | Post-marketing safety surveillance and reevaluation of Motherwort injection:A clinical study of 10094 cases显示文摘OBJECTIVE:To investigate the safety profiles of Motherwort injection(MI).METHODS:A multi-center,prospective and drugderived hospital intensive monitoring method was conducted to assess the safety of MI in real world applications.This study was based on a very large population after the injection was approved and marketed in China.All patients using the injection in participating hospitals were monitored to determine the incidence,pattern,severity and outcome of associated adverse events.RESULTS:The post-marketing surveillance was performed in 10 094 female patients from April to December,2015.The incidence of adverse drug reactions(ADRs) was 0.79‰(8/10 094).Among the 8 patients,the reported adverse events mainly included systemic abnormalities,such as fever,chills and eyelid edema;skin and appendages disorders,such as pruritus and rash;gastrointestinal disorders,such as nausea,abdominal distension and pain;heart rate and rhythm disorders,such as palpitation and increased heart rate.All of these ADRs were mild in severity.CONCLUSION:In this study the ADRs incidence rate of MI is very low,which supports that it is generally safe for use in obstetric and gynecological diseases.However,the total number of 8 ADRs recorded over a relatively short time span seems limited,and the low number of reports could not represent an absolute guarantee of safety. | Cao Shan Zhang Wenhao Zhao Ziwei Heng Mingli Bu Huaien Wang Hongwu Liu Xinghui Wang Zhong Cai Yan Ma Yuyan Cui Shihong Deng Jihong Ding Guifeng Ding Yajuan Dong Linhong Duan Zhentao Fan Ling Fan Yang Fu Fen He Jing Ji Shuying Jin Lin Li Hong Li Hongying Liao Tao Lu Wei Luo Xiucui Lü Zhihui Ma Fengchun Ma Dafeng Shi Tianyun Sun Juying Sun Xiaotong Teng Hong Wang Jinhua Wang Ruihua Wang Ying Wang Zhengling Xi Jie Xu Minjuan Xu Zhihong Yan Qian Yang Cuirong Yang Yimei Yin Jie Yu Jinhua Yuan Wenjun Zhang Guanli Zhang Meihua Zhao Renfeng Zhong Yonghong Zhou Jian | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 4 |
| 18 | Improvement in affinity and thermostability of a fully human antibody against interleukin-17A by yeast-display technology and CDR grafting显示文摘Monoclonal antibodies(m Abs) are widely used in many fields due to their high specificity and ability to recognize a broad range of antigens. IL-17 A can induce a rapid inflammatory response both alone and synergistically with other proinflammatory cytokines. Accumulating evidence suggests that therapeutic intervention of IL-17 A signaling offers an attractive treatment option for autoimmune diseases and cancer. Here, we present a combinatorial approach for optimizing the affinity and thermostability of a novel anti-h IL-17 A antibody. From a large na?ve phage-displayed library, we isolated the anti-IL-17 A m Ab 7 H9 that can neutralize the effects of recombinant human IL-17 A. However, the modest neutralization potency and poor thermostability limit its therapeutic applications. In vitro affinity optimization was then used to generate 8 D3 by using yeast-displayed random mutagenesis libraries.This resulted in four key amino acid changes and provided an approximately 15-fold potency increase in a cell-based neutralization assay. Complementarity-determining regions(CDRs) of 8 D3 were further grafted onto the stable framework of the hu Fv 4 D5 to improve thermostability. The resulting hybrid antibody9 NT/S has superior stabilization and affinities beyond its original antibody. Human fibrosarcoma cellbased assays and in vivo analyses in mice indicated that the anti-IL-17 A antibody 9 NT/S efficiently inhibited the secretion of IL-17 A-induced proinflammatory cytokines. Therefore, this lead anti-IL-17 A m Ab might be used as a potential best-in-class candidate for treating IL-17 A related diseases. | Wei Sun Zhaona Yang Heng Lin Ming Liu Chenxi Zhao Xueying Hou Zhuowei Hu Bing Cuin | 2019 | Acta Pharmaceutica Sinica B2019，9，5： | 4 |
| 19 | (5R)-5-hydroxytriptolide ameliorates anti-glomerular basement membrane glomerulonephritis in NZW mice by regulating Fcγ receptor signaling显示文摘(5R )-5-hydroxytriptolide (LLDT-8 ) 是作为治疗自体免疫的疾病的一个有希望的候选人被识别了并且被显示了在对待鼠科的导致骨胶原的关节炎和豺狼座肾炎有效的新奇 triptolide 类似物。在现在的学习,我们在鼠科的 anti-glomerular 地下室膜(GBM ) 调查了治疗学的效果和 LLDT-8 的行动的可能的机制 glomerulonephritis 模型。NZW 老鼠与兔子 anti-GBM 浆液被注射(500 L, ip ) 。鼠标口头上地与 LLDT-8 被对待(0.125 mg/kg,每隔一天) 或积极控制氢化尼松(2 mg/kg 每天) 为 14 d。血和尿样品以及怒气和肾纸巾为分析被收集。LLDT-8 处理没影响老鼠反兔子抗体的产生。LLDT-8 显著地在 glomerulonephritis 老鼠颠倒了确定的 proteinuria,改进肾的组织病理学说和稀释肾的机能障碍。而且, LLDT-8 在显著地减少的 C3 和 IgG 免职证实的肾禁止了发炎,减少病原的 cytokines TNF- , IL-6, IL-17,和 IFN- 的层次,并且减少在肾的相关 chemokine 表示和白血球渗入。而且, LLDT-8 治疗显著地在肾和脾增加了 FcRIIB 的表示。另外,处理在肾受动器房间的表面上恢复了 FcRIIB 的减少的表示, CD11b + 房间,并且防碍 FcR 依赖的发信号的、特别调停 FcRIIB 的下游的 kinases,例如 BTK。这些结果证明 LLDT-8 改善由调整发信号的 Fc 受体的 anti-GBM glomerulonephritis。 | Qing QI Heng LI Ze-min LIN Xiao-qian YANG Feng-hua ZHU Yu-ting LIU Mei-juan SHAO Lu-yao ZHANG Yan-sheng XU Yu-xi YAN Lan-lan SUN Shi-jun HE Wei TANG Jian-ping ZUO | 2018 | Acta Pharmacologica Sinica2018，39，1： | 4 |
| 20 | Defective mitochondrial function by mutation in THICK ALEURONE 1 encoding a mitochondrion-targeted single-stranded DNA-binding protein leads to increased aleurone cell layers and improved nutrition in rice显示文摘Cereal endosperm comprises an outer aleurone and an inner starchy endosperm.Although these two tissues have the same developmental origin,they differ in morphology,cell fate,and storage product accumulation,with the mechanism largely unknown.Here,we report the identification and characterization of rice thick aleurone 1(ta1)mutant that shows an increased number of aleurone cell layers and increased contents of nutritional factors including proteins,lipids,vitamins,dietary fibers,and micronutrients.We identified that the TA1 gene,which is expressed in embryo,aleurone,and subaleurone in caryopses,encodes a mitochondrion-targeted protein with single-stranded DNA-binding activity named OsmtSSB1.Cytological analyses revealed that the increased aleurone cell layers in ta1 originate from a developmental switch of subaleurone toward aleurone instead of starchy endosperm in the wild type.We found that TA1/OsmtSSB1 interacts with mitochondrial DNA recombinase RECA3 and DNA helicase TWINKLE,and downregulation of REC A3 or TWINKLE also leads to ta1-like phenotypes.We further showed that mutation in TA1/OsmtSSB1 causes elevated illegitimate recombinations in the mitochondrial genome,altered mitochondrial morphology,and compromised energy supply,suggesting that the OsmtSSB1-mediated mitochondrial function plays a critical role in subaleur one cell-fate determination in rice. | Dong-Qi Li Xiao-Ba Wu Hai-Feng Wang Xue Feng Shi-Juan Yan Sheng-Yang Wu Jin-Xin Liu Xue-Feng Yao Ai-Ning Bai Heng Zhao Xiu-Fen Song Lin Guo Shi-Yong Zhang Chun-Ming Liu | 2021 | Molecular Plant2021，14，8： | 4 |
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