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| 1 | Effect of dietary supplementation of xylanase on apparent ileal digestibility of nutrients,viscosity of digesta,and intestinal morphology of growing pigs fed corn and soybean meal based diet显示文摘This study was to determine apparent ileal digestibility of acid detergent fiber(ADF), neutral detergent fiber(NDF), dry matter(DM), energy, organic matter(OM), crude ash, digesta viscosity, and gut morphology in nursery pigs fed diets containing xylanase(Lohmann Animal Nutrition GmbH, Cuxhaven, Germany). The diet(61% corn, 35% soybean meal, 1% poultry fat, and 3% minerals and vitamins) was mixed with 3 levels of xylanase(0,700, and 1400 LXU/kg). Thirty-six barrows(17.6 ± 3.3 kg) received one of 3 treatment diets based on a randomized complete block design with the initial body weight(BW) as a block. Pigs were individually housed and received experimental diets twice daily(0700 and 1700 h) at a fixed amount based on BW of pigs(0.09 x BW^(0.75) kg). Pigs were fed diets for 10 d, and chromium oxide(0.3%) was added to the diets from d 6 as an indigestible external marker. Pigs were euthanized at the end of d 10 for the collection of digesta and tissues. Jejunal digesta were centrifuged to measure viscosity using a viscometer(Brookfield Engineering Laboratories, Stoughton, MA). Diets and freeze-dried ileal digesta were used to measure ADF, NDF, and chromium to calculate apparent ileal digestibility of ADFand NDF. Villus height and crypt depth of jejunum were measured using a microscope(Fisher Scientific, Hampton, NH). Data were analyzed using polynomial contrasts in the MIXED procedure of SAS version 9.3(SAS Inc., Cary, NC, USA). Morphological measurements and ileal ADF digestibility were not affected by increasing xylanase. However, increasing xylanase supplementation from 0 to 1400 LXU/kg enhanced ileal digestibility of NDF(P < 0.042, linear) from 27.9 to 40.3%,DM(P < 0.006, linear) from 55.4 to 64.6%, OM(P < 0.006, linear) from 59.2 to 67.7%, and energy(P < 0.003,linear) from 58.8 to 68.0%. Viscosity of jejunal digesta decreased(P < 0.023) in a non-linear manner from 2.9 to 2.5 centipoises(cP). In conclusion, the usage of xylanase in corn and soybean meal based pig diets linearly enhanced digestibility of nutrients and affected viscosity of digesta in a non-linear manner. | Adsos Adami Passos Inkyung Park Peter Ferket Elke von Heimendahl Sung Woo Kim | 2015 | Animal Nutrition2015，，1： | 10 |
| 2 | Effect of silymarin on biochemical indicators in patients with liver disease: systematic review with meta-analysis显示文摘AIM To evaluate the effect of silymarin on the serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST) and gamma glutamyl transpeptidase(γGT) in patients with liver diseases. METHODS A systematic review with meta-analysis of ramdomized and controlled clinical trials was performed,evaluating the effects of sylimarin in patients with hepatic diseases,published by January 31,2016. Clinical trials were sought on the basis of The Cochrane Central Register of Controlled Trials in the Cochrane Library,Pub Med/Medline,Scopus,Web of Science,Lilacs and Clinical Trials. The trials with adult and elderly patients of both sexes,with Liver Diseases who took oral silymarin supplementation,as extract or isolated,as well as Silymarin combined with other nutrients,were included. The trials should provide information about the intervention,such as dosages and detailing of the product used,besides the mean and standard deviation of serum levels of ALT,AST and γGT of the baseline and at the end of the intervention.RESULTS An amount of 10904 publications were identified. From those,only 17 were included in the systematic review and 6 in the meta-analysis,according to the used selection criteria. In this meta-analysis,the results indicated a reduction of 0.26 IU/m L(95%CI:-0.46-0.07,P = 0.007) at the level of ALT and 0.53 IU/m L(95%CI:-0.74-0.32,P = 0.000) at the serum levels of AST after using the silymarin,both,statistically significant,but with no clinical relevance. There was no significant change in the γGT levels. Subgroup analyzes were also performed for the biochemical markers in relation to the type of intervention,whether silymarin isolated or associated with other nutrients and the time of intervention(whether ≥ 6 mo or < 6 mo). Significant differences were not found. The evaluated studies presented a high degree of heterogeneity and low methodological quality in the carried out analysis. CONCLUSION Silymarin minimally reduced,but without clinical relevance,the serum levels of ALT and AST. It is necessary to carry out studies with more appropriate methodological designs. | Camila Ribeiro de Avelar Emile Miranda Pereira Priscila Ribas de Farias Costa Rosangela Passos de Jesus Lucivalda Pereira Magalhaes de Oliveira | 2017 | World Journal of Gastroenterology2017，23，27： | 5 |
| 3 | Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?显示文摘AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of colitis.METHODS:MSCs were isolated from C57BL/6 mouse adipose tissue.MSC cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular markers.Experimental acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to 7.Colitis mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and 5.The disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and anus.To compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological changes.Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit.Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling assay.RESULTS:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and inflammation.An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells.This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in apoptotic T cells was observed after intravenous,but not intraperitoneal,MSC infusion,suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation(P=0.027).CONCLUSION:Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy. | Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 4 | Resveratrol attenuates oxidative stress and prevents steatosis and hypertension in obese rats programmed by early weaning显示文摘 | Juliana G. Franco Patrícia C. Lisboa Natália S. Lima Taline A.S. Amaral Nayara Peixoto-Silva Angela C. Resende Elaine Oliveira Magna C.F. Passos Egberto G. Moura | 2013 | The Journal of Nutritional Biochemistry2013，，6： | 2 |
| 5 | 动态文化为骨头工程作为脚手架在 freeze-dried 骨头上改进 MSC 粘附显示文摘 AIM:To investigate the interaction between mesenchymal stem cells(MSCs) and bone grafts using two different cultivation methods:static and dynamic.METHODS:MSCs were isolated from rat bone marrow.MSC culture was analyzed according to the morphology,cell differentiation potential,and surface molecular markers.Before cell culture,freeze-dried bone(FDB) was maintained in culture for 3 d in order to verify culture medium pH.MSCs were co-cultured with FDB using two different cultivation methods:static co-culture(two-dimensional) and dynamic co-culture(threedimensional).After 24 h of cultivation by dynamic or static methods,histological analysis of Cell adhesion on FDB was performed.Cell viability was assessed by the Trypan Blue exclusion method on days 0,3 and 6 after dynamic or static culture.Adherent cells were detached from FDB surface,stained with Trypan Blue,and quantified to determine whether the cells remained on the graft surface in prolonged non-dynamic culture.Statistical analyses were performed with SPSS and a P < 0.05 was considered significant.RESULTS:The results showed a clear potential for adipogenic and osteogenic differentiation of MSC cultures.Rat MSCs were positive for CD44,CD90 and CD29 and negative for CD34,CD45 and CD11bc.FDBs were maintained in culture for 3 d and the results showed there was no significant variation in the culture medium pH with FDB compared to pure medium pH(P > 0.05).In histological analysis,there was a significant difference in the amount of adhered cells on FDB between the two cultivation methods(P < 0.05).The MSCs in the dynamic co-culture method demonstrated greater adhesion on the bone surface than in static co-culture method.On day 0,the cell viability in the dynamic system was significantly higher than in the static system(P < 0.05).There was a statistical difference in cell viability between days 0,3 and 6 after dynamic culture(P < 0.05).In static culture,cell viability on day 6 was significantly lower than on day 3 and 0(P < 0.05).CONCLUSION:An alternative cultivation method was developed to improve the MSCs adhesion on FDB,demonstrating that dynamic co-culture provides a superior environment over static conditions. | Fabiany da Costa Gonalves Ana Helena da Rosa Paz Priscila Schmidt Lora Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima | 2012 | World Journal of Stem Cells2012，4，2： | 2 |
| 6 | Methane Direct Conversion on Mo/ZSM-5 Catalysts Modified by Pd and Ru显示文摘到在甲烷的 dehydroaromatization 使用的 Mo/HZSM-5 催化剂的 Ru 和 Pd 的增加的效果被调查。催化测试和规划温度的氧化结果证明基于 Pd 的催化剂对萘更选择并且承受了强壮的释放。因为 Mo2C 表面的保护的机制, Ru 的存在可能改进了这项活动和稳定性,与在碳质沉积的减少。给词调音:甲烷;Pd;Ru;瞬间;ZSM-5; | Priscila Dias Sily Fabio Bellot Noronha Fabio Barboza Passos | 2006 | Journal of Natural Gas Chemistry2006，15，2： | 2 |
| 7 | Information-governing dynamics ot attacker-defender interactions in youth rugby union显示文摘 | Passos P Araujo D Davids K | 2008 | Journal of Sports Sciences2008，26，： | 1 |
| 8 | Renal arteries:anatomic study for surgi-cal and radiological practice显示文摘 | Sampaio FJ Passos MA | 1992 | Surg Radiol Anat1992，14，2： | 1 |
| 9 | Simulation of an intermittent adsorptive solar cooling system 显示文摘 | Passos E F Escobedo J F | 1989 | Solar Energy1989，42，2： | 1 |
| 10 | Candida colonization in intensive care unit patients'urine显示文摘 | Passos XS Sales WS Maciel PJ | 2005 | Mem Inst Oswaldo Cruz2005，100，8： | 1 |
| 11 | Analysis of the cost-effectiveness of thrombolysis with alteplase in stroke 显示文摘 | Ara6jo DV Teich V Passos RB | 2010 | Arq Bras Cardiol2010，95，1： | 1 |
| 12 | Impaired reflexcontrol of intestinal gas transit in patients with abdominal bloa-ting显示文摘 | PASSOS MC SERRA J AZPIROZ F | 2005 | Gut2005，54，3： | 1 |
| 13 | Differential immune regulation of activated T cells between cutaneous and mucosal leishmaniasis as a model for pathogenesis显示文摘 | Carvalho LP Passos S Bacellar O | | 0，，05： | 1 |
| 14 | Biogas production from microalgae grown in wastewater:Effect of microwave pretreatment显示文摘 | Passos F Sole M Garcia J | 2013 | Applied Energy2013，，108： | 1 |
| 15 | Structure - function relationships of immunostimulatory polysaccharides: A review显示文摘 | Ferreira S S Passos C P Madureira P | 2015 | Carbohydrate Polymers2015，132，： | 1 |
| 16 | Agressive inflammatory myofibroblastic tumor of the liver with underlying schistosomiasis:A case report显示文摘Inflammatory myofibroblastic tumor(IMT)occurs infrequently in the liver.It is controversial whether it represents a low grade mesenchymal neoplasm or a reactive inflammatory lesion.Local recurrence and metastasis are rare and some tumors are associated with infectious agents.We report on a case of a large and partially resected IMT with local recurrence and diaphragm and kidney infiltration detected on routine surveillance two years later.Histologically,the tumor showed spindle cells without atypia,mitosis or necrotic areas in a myxoid and collagenized background with inflammatory cells.In the liver portal tracts,granulomatous lesions with viable eggs of Schistosoma mansoni were identified.Immunohistochemistry demonstrated spindle cells which were smooth-muscle actin and vimentin positive.In conclusion,this case points out that these histological patterns do not predict the aggressive biological behavior of the lesion.A reason for the recurrence and the infiltration may be incomplete tumor resection.Further investigation is necessary in order to better clarify an infectious cause in some IMTs. | Vera Lucia Pannain Juliana Vial Passos Ariovaldo da Rocha Filho Cristiane Villela-Nogueira Adriana Caroli-Bottino | 2010 | World Journal of Gastroenterology2010，16，33： | 1 |
| 17 | Peritoneal fluid modulates thesperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein显示文摘 | Brugnara L Facin A C | 1999 | Braz J Med Biol Res1999，32，1： | 1 |
| 18 | Characterization and catalytic activity of bimetallic Pt-In/Al2O3 and Pt-Sn/Al2O3 catalysts显示文摘 | Aranda D A G Schmal M | 1998 | Journal of Catalysis1998，178，2： | 1 |
| 19 | Postnatal early overfeeding induces hypothalamic higher SOCS3 expression and lower STAT3 activity in adult rats显示文摘 | Rodrigues AL De Moura EG Passos MC | 2011 | J Nutr Biochem2011，22，： | 1 |
| 20 | The H63D genetic variant of the HFE gene is independently associated with the virological response to interferon and ribavirin therapy in chronic hepatitis C显示文摘 | Marcos V. Carneiro Fernanda F. Souza Andreza C. Teixeira José F.C. Figueiredo Marcia G. Villanova Marie Secaf Afonso Passos Leandra N.Z. Ramalho Fabiana P. Carneiro Sérgio Zucoloto Ana L. Candolo Martinelli | 2010 | European Journal of Gastroenterology & Hepatology2010，，10： | 1 |
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