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| 1 | New means to monitor the effect of glucocorticoid therapy in children显示文摘AIM:To study the individual effects of glucocorticoid (GC) therapy on the state ofimmune activation in patient serum.METHODS:We developed a novel assay in which the effect of corticosteroid-treated patient serum on healthy donor peripheral blood mononuclear cells (target cells) was studied,with a panel of markers for effector [interferon (IFN)γ and interleukin (IL)-5] and regulatory T cells (FOXP3 and glucocorticoid-induced tumor necrosis factor receptor,GITR).The study group comprised 19 children with inflammatory bowel disease.The individual effect of patient serum on target cells was analyzed prior to GC therapy and 2 wk later.RESULTS:The effect of GC therapy mediated by patient serum was seen as a decrease in the target cells expression of regulatory T-cell-related markers GITR (median suppression 24%,range of suppression 1%-63%,in 2 cases increase of 6% and 77%,P < 0.01 for mitogen-activated target cells) and FOXP3 (median suppression 33%,range of suppression 0%-79%,in one case an increase of 173%,P < 0.05 for resting cells),and secretion of IFNγ [from a mean of 87 700 pg/mL (SD 33 900 pg/mL) to 60 900 pg/mL (SD 44 200 pg/mL) in mitogen-activated target cells,13 of the cases showed a decrease,P < 0.01].The total or weight-related prednisolone dose did not correlate with the patient-seruminduced changes in the target cell markers.CONCLUSION:GC response could be monitored at an individual level by studying the effect of patient serum on signaling pathways of target immune cells. | Hanne Rintamki Harri M Salo Outi Vaarala Kaija-Leena Kolho | 2010 | World Journal of Gastroenterology2010，16，9： | 4 |
| 2 | Reliability issue of replacing solder with conductive adhesives in powder modules 显示文摘 | Rusanen Outi Lenkker Jaako | 1995 | IEEE Trans Compon Packg Manuf Tech1995，18，2： | 2 |
| 3 | Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution显示文摘AIM:To optimize a xeno-free cryopreservation protocol for primary human hepatocytes.METHODS:The demand for cryopreserved hepatocytes is increasing for both clinical and research purposes.Despite several hepatocyte cryopreservation protocols being available,improvements are urgently needed.We first compared controlled rate freezing to polystyrene box freezing and did not find any significant change between the groups.Using the polystyrene box freezing,we compared two xeno-free freezing solutions for freezing of primary human hepatocytes:a new medium(STEM-CELLBANKER,CB),which contains dimethylsulphoxide(DMSO) and anhydrous dextrose,both permeating and non-permeating cryoprotectants,and the frequently used DMSO-University of Wisconsin(DMSOUW) medium.The viability of the hepatocytes was assessed by the trypan blue exclusion method as well as a calcein-esterase based live-dead assay before and after cryopreservation.The function of the hepatocytes was evaluated before and after cryopreservation by assessing enzymatic activity of 6 major cytochrome P450 isoforms(CYPs):CYP1A2,CYP2C9,CYP2C19,CYP2D6,CYP3A4 and CYP3A7.RESULTS:The new cryoprotectant combination preserved hepatocyte viability significantly better than the standard DMSO-UW protocol(P < 0.01).There was no significant difference in viability estimation between both the trypan blue(TB) and the Live-Dead Assay methods.There was a correlation between viability of fresh hepatocytes and the difference in cell viability between CB and DMSO protocols(r 2 = 0.69) using the TB method.However,due to high within-group variability in the activities of the major CYPs,any statistical between-group differences were precluded.Cryopreservation of human hepatocytes using the cryoprotectant combination was a simple and xeno-free procedure yielding better hepatocyte viability.Thus,it may be a better alternative to the standard DMSO-UW protocol.Estimating CYP activities did not seem to be a relevant way to compare hepatocyte function between different groups due to high normal variability between different liver samples.CONCLUSION:The cryoprotectant combination may be a better alternative to the standard DMSO-UW protocol in primary human hepatocyte cryopreservation. | Mohammed Saliem Frida Holm Rosita Bergstrm Tengzelius Carl Jorns Lisa-Mari Nilsson Bo-Gran Ericzon Ewa Ellis Outi Hovatta | 2012 | World Journal of Hepatology2012，4，5： | 2 |
| 4 | Human trypsinogens in the pancreas and in cancer显示文摘 | Itkonen Outi | 2010 | Scand J Clin Lab invest2010，70，2： | 1 |
| 5 | An Activity-Based Costing Model for Logistics Operations of Manufacturers and Wholesalers显示文摘 | OUTI M | 2000 | International Journal of Logistics:Research & Applications2000，3，1： | 1 |
| 6 | Krause Vapor-Liquid and Chemical Reaction Equilibria in the Synthesis of 2-Methoxy-2-methylbutane (TAME) 显示文摘 | Iiisa K Rihko Juha A Linnekoski A Outi I | 2000 | Chem Eng Data2000，，45： | 1 |
| 7 | Stem modification and cluster transfer in Modern Hebrew显示文摘 | Outi Bat-El | | Natural Language and Linguistic Theory0，，： | 1 |
| 8 | Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor显示文摘 | Heidi Koivisto Marjukka Hyv?rinen Anne-Marie Str?mberg Jose Inzunza Eija Matilainen Milla Mikkola Outi Hovatta Heli Teerijoki | 2004 | Reproductive BioMedicine Online2004，，3： | 1 |
| 9 | The gene for polycomb group protein enhancer of zeste homolog 2 (EZH2) is amplified in late‐stage prostate cancer显示文摘 | Outi R.Saram?ki Teuvo L. J.Tammela Paula M.Martikainen Robert L.Vessella TapioVisakorpi | 2006 | Genes Chromosom Cancer2006，，7： | 1 |
| 10 | Inflammatory and Anti-inflammatory Responsiveness of Surfactant Proteins in Fetal and Neonatal Rabbit Lung 显示文摘 | Outi Vayrynen Virpi Glumoff Mikko Hallman | 2004 | Pediatric Research2004，55，1： | 1 |
| 11 | Exome sequencing in diagnostic evaluation of colorectal cancer predisposition in young patients显示文摘 | Tomas Tanskanen Alexandra E. Gylfe Riku Katainen Minna Taipale Laura Renkonen-Sinisalo Jukka-Pekka Mecklin Heikki J?rvinen Sari Tuupanen Outi Kilpivaara Pia Vahteristo Lauri A. Aaltonen | 2013 | Scandinavian Journal of Gastroenterology2013，，6： | 1 |
| 12 | Germ cell apoptosis after treatment of eryptorehidism with human ehorionie gonadotropin is associated with Impaired reproductive function in the adult 显示文摘 | Leo Dunkel Seppo Taskinen Outi Hovatta | 1997 | J Clin Invest1997，100，9： | 1 |
| 13 | An Activity-based costing model for logistics operations of manufacturers and wholesalers显示文摘 | Outi M | 2000 | International Journal of Logistics: Research and Applications2000，3，1： | 1 |
| 14 | Infiltration of Foxp3- and Toll-like Receptor-4–positive Cells in the Intestines of Children With Food Allergy显示文摘 | Mia Westerholm-Ormio Outi Vaarala Minna Tiittanen Erkki Savilahti | 2010 | Journal of Pediatric Gastroenterology and Nutrition2010，，4： | 1 |
| 15 | The effect of gradual distraction of the mandible on the sheep temporomandibular joint显示文摘 | Terhi Karaharju-Suvanto Jari Peltonen Outi Laitinen Arvi Kahri | 1996 | International Journal of Oral & Maxillofacial Surgery1996，，2： | 1 |
| 16 | Research resource:interactome of human embryo implantation:identification of gene expression pathways,regulation,and integrated regulatory networks显示文摘 | Signe A Jüri R Outi H | 2011 | Mol Endocrinol2011，26，1： | 1 |
| 17 | Bacterial biofilm formation on polyvinyl chloride, polyethylene and stainless steel exposed to ozonated water 显示文摘 | Outi M Zacheus Eila K Iivanainen | 2000 | Wat Res2000，，1： | 1 |
| 18 | Insight to sulfur species in the hydrodeoxygenation of aliphatic esters over sulfided NiMo/γ-Al 2 O 3 catalyst显示文摘 | Eeva-Maija Ryymin Maija L. Honkela Tuula-Riitta Viljava A. Outi I. Krause | 2009 | Applied Catalysis A General2009，，1： | 1 |
| 19 | Patient-controlled sedation with propofol and remifentanil for ERCP: a randomized, controlled study显示文摘 | Maxim Mazanikov Marianne Udd Leena Kyl?np?? Outi Lindstr?m Pekka Aho Jorma Halttunen Martti F?rkkil? Reino P?yhi? | 2011 | Gastrointestinal Endoscopy2011，，2： | 1 |
| 20 | Fecal Microbiota Composition Differs Between Children With [Beta]-Cell Autoimmunity and Those Without显示文摘 | de Goffau Marcus C Luopaj?rvi Kristiina Knip Mikael Ilonen Jorma Ruohtula Terhi H?rk?nen Taina Orivuori Laura Hakala Saara Welling Gjalt W Harmsen Hermie J Vaarala Outi | 2013 | Diabetes2013，，4： | 1 |
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