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| 1 | Sleep and Cognitive Abnormalities in Acute Minor Thalamic Infarction显示文摘In order to characterize sleep and the cognitive patterns in patients with acute minor thalamic infarction(AMTI), we enrolled 27 patients with AMTI and 12 matched healthy individuals. Questionnaires about sleep and cognition as well as polysomnography(PSG) were performed on days 14 and 90 post-stroke. Compared to healthy controls, in patients with AMTI, hyposomnia was more prevalent; sleep architecture was disrupted as indicated by decreased sleep efficiency, increased sleep latency, and decreased non-rapid eye movement sleep stages 2 and 3;more sleep-related breathing disorders occurred; and cognitive functions were worse, especially memory. While sleep apnea and long-delay memory recovered to a large extent in the patients, other sleep and cognitive function deficit often persisted. Patients with AMTI are at an increased risk for hyposomnia, sleep structure disturbance,sleep apnea, and memory deficits. Although these abnormalities improved over time, the slow and incomplete improvement suggest that early management should be considered in these patients. | Wei Wu Linyang Cui Ying Fu Qianqian Tian Lei Liu Xuan Zhang Ning Du Ying Chen Zhijun Qiu Yijun Song Fu-Dong Shi Rong Xue | 2016 | Neuroscience Bulletin2016，32，4： | 28 |
| 2 | Liproxstatin-1 is an effective inhibitor of oligodendrocyte ferroptosis induced by inhibition of glutathione peroxidase 4显示文摘Our previous studies showed that ferroptosis plays an important role in the acute and subacute stages of spinal cord injury.High intracellular iron levels and low glutathione levels make oligodendrocytes vulnerable to cell death after central nervous system trauma.In this study,we established an oligodendrocyte(OLN-93 cell line)model of ferroptosis induced by RSL-3,an inhibitor of glutathione peroxidase 4(GPX4).RSL-3 significantly increased intracellular concentrations of reactive oxygen species and malondialdehyde.RSL-3 also inhibited the main antiferroptosis pathway,i.e.,SLC7A11/glutathione/glutathione peroxidase 4(xCT/GSH/GPX4),and downregulated acyl-coenzyme A synthetase long chain family member 4.Furthermore,we evaluated the ability of several compounds to rescue oligodendrocytes from ferroptosis.Liproxstatin-1 was more potent than edaravone or deferoxamine.Liproxstatin-1 not only inhibited mitochondrial lipid peroxidation,but also restored the expression of GSH,GPX4 and ferroptosis suppressor protein 1.These findings suggest that GPX4 inhibition induces ferroptosis in oligodendrocytes,and that liproxstatin-1 is a potent inhibitor of ferroptosis.Therefore,liproxstatin-1 may be a promising drug for the treatment of central nervous system diseases. | Bao-You Fan Yi-Lin Pang Wen-Xiang Li Chen-Xi Zhao Yan Zhang Xu Wang Guang-Zhi Ning Xiao-Hong Kong Chang Liu Xue Yao Shi-Qing Feng | 2021 | Neural Regeneration Research2021，16，3： | 19 |
| 3 | Fault Diagnosis Approach of Local Ventilation System in Coal Mines Based on Multidisciplinary Technology显示文摘In order to reduce the probability of fault occurrence of local ventilation system in coal mine and prevent gas from exceeding the standard limit, an approach incorporating the reliability analysis, rough set theory, genetic algorithm (GA), and intelligent decision support system (IDSS) was used to establish and develop a fault diagnosis system of local ventilation in coal mine. Fault tree model was established and its reliability analysis was performed. The algorithms and software of key fault symptom and fault diagnosis rule acquiring were also analyzed and developed. Finally, a prototype system was developed and demonstrated by a mine instance. The research results indicate that the proposed approach in this paper can accurately and quickly find the fault reason in a local ventilation system of coal mines and can reduce difficulty of the fault diagnosis of the local ventilation system, which is significant to decrease gas exploding accidents in coal mines. | GONG Xiao-yan XUE He TAO Xin-li HU Ning | 2006 | Journal of China University of Mining and Technology2006，16，3： | 18 |
| 4 | Probucol attenuates atrial autonomic remodeling in a canine model of atrial fibrillation produced by prolonged atrial pacing显示文摘背景我们假设氧化应力和发炎可以玩的那增加的 atrial 在 atrial 神经在 atrial 纤维性颤动(AF ) 期间发芽和异构的同情的 hyperinnervation 的一个重要角色。测试假设,我们检验了有 probucol 的抗氧化剂和反煽动性的处理是否稀释在 AF 的一个犬的模型的自治改变由延长快速的正确 atrial 踱步生产了的 atrial。21 条狗被划分成一个假冒操作组,一个控制组和一个 probucol 组的方法。在控制组和 probucol 组的狗经历了每分钟在 400 敲击踱步的正确 atrial 因为在踱步直到踱步的快速的 atrial 停止了以前,在 probucol 的 6 个星期,和那些 1 个星期组织收到的 probucol。在 6 星期的快速的 atrial 踱步以后,包括的一般性质离开了 atrial AF 的结构和功能, atrial hemodynamics 和 inducibility 和持续时间在所有这些组被测量。Atrial 氧化压力标记和浆液 C 反应的蛋白质(CRP ) 集中被估计。神经在正确 atrial 发芽和同情的神经分布的度前面的墙(RAAW ) 和左 atrial 前面的墙(LAAW ) 被 immunohistochemistry 确定, atdal 新肾上素内容也被检测。在 RAAW 和 LAAW 的 Atrial 贝它神经生长因素(beta-NGF ) mRNA 和蛋白质表示被即时量的 RT-PCR 并且分别地的西方的弄污估计。结果 Atrial tachypacing 导致了重要神经发芽和异构的同情的 hyperinnervation,并且神经发芽和 hyperinnervation 的大小比在 LAAW 在 RAAW 是更高的。Atrial beta-NGF mRNA 和蛋白质层次显著地在 RAAW 和 LAAW 被增加,并且 beta-NGF 表示的 upregulation 比在在控制组的 LAAW 在 RAAW 是更大的。beta-NGF 蛋白质水平断然在所有组与同情的神经的密度被相关。Probucol 减少了氧化应力由 atrial tachypacing 引起了的 CRP 集中和稀释 atrial 的增加。另外, probucol 能有效地禁止 atrial beta-NGF upregulation,显著地稀释 atrial 神经发芽和异构的同情的 hyperinnervation,并且戏剧性地减少 AF 的 inducibility 和持续时间。beta-NGF 的 atrial 在表示上可能由增加的氧化应力和发炎引起了的结论可以是位于 atrial 下面的主要机制在 AF 期间的自治改变。Probucol 稀释 atrial 由它的抗氧化剂和反煽动性的行动的可能的自治改变。 | GONG Yong-tai LI Wei-min LI Yue YANG Shu-sen SHENG Li YANG Ning SHAN Hong-bo XUE Hong-jie LIU Wei YANG Bao-feng DONG De-li LI Bao-xin | 2009 | Chinese Medical Journal2009，，1： | 15 |
| 5 | Growth and Common Prosperity in China显示文摘This paper introduces a decomposition method that quantifies the contributions to common prosperity of labor market performance and social policies and extends the idea of shared prosperity to a new measure of inequity in opportunities.The resulting common prosperity indices and opportunity equality indices are then applied to five waves of the Chinese Household Income Project data from 1988 to 2018.This paper shows that the labor market performance and social policies have been improving over the last 30 years and have helped China move towards common prosperity for everyone.The indices developed in this paper allow us to quantify the extent of shared prosperity that a country has achieved and to carry out empirical studies on which policy is working and which is not.It can also help us identify the fundamental causes of inequality and aid us in achieving equality in opportunity among all members of society. | Nanak Kahwani Xiaobing Wang Ning Xue Peng Zhan | 2022 | China & World Economy2022，30，1： | 13 |
| 6 | Comparative study of minimally invasive versus open esophagectomy for esophageal cancer in a single cancer center显示文摘 | Mu Juwei Yuan Zuyang Zhang Baihua Li Ning Lyu Fang Mao Yousheng Xue Qi Gao Shugeng Zhao Jun Wang Dali Li Zhishan Gao Yushun Zhang Liangze Huang Jinfeng Shao Kang Feng Feiyue Zhao Liang Li Jian Cheng Guiyu Sun Kelin He Jie | 2014 | Chinese Medical Journal2014，，4： | 13 |
| 7 | Dapper1 promotes autophagy by enhancing the Beclinl -Vps34-Atg14L complex formation显示文摘 | Benvu Ma Weipeng Cao Wenxia Li Chan Gao Zhen Qi Yan Zhao Jun Du Hua Xue Junya Peng Jun Wen Hua Chen Yuanheng Ning Lei Huang Hong Zhang Xiang Gao Li Yu Ye-Guang Chen | 2014 | Cell Research2014，24，8： | 12 |
| 8 | A Matched Comparison Study of Uniportal Versus Triportal Thoracoscopic Lobectomy and Sublobectomy for Early-stage Nonsmall Cell Lung Cancer显示文摘 | Ju-Wei Mu Shu-Geng Gao Qi Xue Jun Zhao Ning Li Kun Yang Kai Su Zhu-Yang Yuan Jie He | 2015 | Chinese Medical Journal2015，，20： | 12 |
| 9 | Mitosin/CENP-F is a conserved kinetochore protein subjected to cyto plasmic dynein-mediated poleward transport显示文摘Mitosin/CENP-F is a human nuclear protein transiently associated with the outer kinetochore plate in M phase and is involved in M phase progression. LEK1 and CMF1, which are its murine and chicken orthologs, however, are implicated in muscle differentiation and reportedly not distributed at kinetochores.We therefore conducted several assays to clarify this issue. The typical centromere staining patterns were observed in mitotic cells from both human primary culture and murine, canine, and mink cell lines. A C-terminal portion of LEK1 also conferred centromere localization. Our analysis further suggests conserved kinetochore localization of mammalian mitosin orthologs. Moreover, mitosin was associated preferentially with kinetochores of unaligned chromosomes. It was also constantly transported from kinetochores to spindle poles by cytoplasmic dynein. These properties resemble those of other kinetochore proteins important for the spindle checkpoint, thus implying a role of mitosin in this checkpoint. Therefore, mitosin family may serve as multifunctional proteins involved in both mitosis and differentiation. | ZHEN YE YANG, JING GUO, NING LI, MIN QIAN, SHENG NIAN WANG, XUE LIANG ZHULaboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China | 2003 | Cell Research2003，13，4： | 11 |
| 10 | Association Between Serum Uric Acid and Prevalence of Type 2 Diabetes Diagnosed using HbA1c Criteria Among Chinese Adults in Qingdao,China显示文摘Objective To determine whether elevated serum uric acid(UA)levels are associated with type 2 diabetes diagnosed using Hb A1 c levels among Chinese adults.Methods We conducted two population-based cross-sectional studies in Qingdao in China in 2006 and 2009.A total of 6894(39.4% men)subjects aged 35-74 years were included in the data analysis.Newly diagnosed diabetes was defined as Hb A1 c level of ≥6.5%,and prediabetes was classified as Hb A1 c level between 5.7% and 6.4% according to the International Diabetes Federation criteria.Multivariate logistic regression was employed to assess the association between UA and prevalence of type 2 diabetes defined using Glycated hemoglobin A1c(Hb A1 c levels.Results Subjects with prediabetes had higher UA levels than those with normal glucose tolerance,newly diagnosed diabetes,and known diabetes,with corresponding values of 325.1(82.5)μmol/L,310.9(84.2)μmol/L,291.3(81.7)μmol/L,305.2(83.6)μmol/L,respectively(P<0.001 for all comparisons).Binary logistic regression analysis showed that UA was a possible predictor for the prevalence of type 2 diabetes diagnosed using Hb A1 c levels,and the second quartile of UA levels had a higher odds ratio(OR:4.088;95% CI:2.900-5.765)for Hb A1 c than the other quartiles after adjusting for age,body mass index,sex,marital status,education,income,alcohol consumption,smoking,and cardiometabolic parameters.Conclusion Serum UA is significantly associated with type 2 diabetes diagnosed using Hb A1 c levels,independent of other cardiometabolic parameters. | XUE Bai TAN Ji Bin NING Feng SUN Jian Ping ZHANG Ke Yi LIU Li WANG Shao Jie ZHANG Dong Feng QIAO Qing PANG Zeng Chang | 2015 | Biomedical and Environmental Sciences2015，28，12： | 9 |
| 11 | Neuroprotective effect of deferoxamine on erastin-induced ferroptosis in primary cortical neurons显示文摘The iron chelator deferoxamine has been shown to inhibit ferroptosis in spinal cord injury.However,it is unclear whether deferoxamine directly protects neurons from ferroptotic cell death.By comparing the survival rate and morphology of primary neurons and SH-SY5Y cells exposed to erastin,it was found that these cell types respond differentially to the duration and concentration of erastin treatment.Therefore,we studied the mechanisms of ferroptosis using primary cortical neurons from E16 mouse embryos.After treatment with 50μM erastin for 48 hours,reactive oxygen species levels increased,and the expression of the cystine/glutamate antiporter system light chain and glutathione peroxidase 4 decreased.Pretreatment with deferoxamine for 12 hours inhibited these changes,reduced cell death,and ameliorated cellular morphology.Pretreatment with the apoptosis inhibitor Z-DEVD-FMK or the necroptosis inhibitor necrostain-1 for 12 hours did not protect against erastin-induced ferroptosis.Only deferoxamine protected the primary cortical neurons from ferroptosis induced by erastin,confirming the specificity of the in vitro ferroptosis model.This study was approved by the Animal Ethics Committee at the Institute of Radiation Medicine of the Chinese Academy of Medical Sciences,China(approval No.DWLL-20180913)on September 13,2018. | Yan Zhang Bao-You Fan Yi-Lin Pang Wen-Yuan Shen Xu Wang Chen-Xi Zhao Wen-Xiang Li Chang Liu Xiao-Hong Kong Guang-Zhi Ning Shi-Qing Feng Xue Yao | 2020 | Neural Regeneration Research2020，15，8： | 9 |
| 12 | Genetic variants in three genes and smoking show strong associations with susceptibility to exudative age-related macular degeneration in a Chinese population显示文摘背景现在的学习被承担在三基因复制代表性的多型性的协会(补充因素 H ( CFH ),补充因素 B ( BF )和 HtrA 丝氨酸 peptidase 1 ( HTRA1 ))与 exudative 在一张哈特汉语人口的年龄相关的有斑点的退化( AMD ),并且如果可修改的环境因素影响 AMD ,到测试,危险性在这些基因与遗传型的不同类型联系了。年龄,性和种族匹配盒子控制学习的方法被进行到包括在在 BF 的 CFH 和在在 144 个 exudative AMD 病人和 126 正常的 HTRA1 基因的 rs11200638 的 rs1061170 和 rs1410996 的代表性的单个 neucleotide 多型性(SNP ) loci 控制使用 PCR-RFLP 和 directresequencing 的遗传型。人口统计的特征和行为的风险因素也被记录。突变而产生之遗传并且单个 SNP 的 genotypic 协会和有二 loci 的联合协会被执行。基因基因和基因环境相互作用用 multivariate 被分析非有条件的逻辑回归分析。结果为在情况和控制中的 CFH Y402H (rs1061170 ) 的 C 风险等位基因频率分别地是 12.5% 和 5.4% ,它是比在白种人(P0.001 ) 的那些低得多的。与 TT 同型结合的遗传型相比, CT 异质接合的遗传型断然与机会比率与 AMD 被联系(或) 3.23 (1.36-5.07 ) 。然而,人口 C 等位基因的可归因的风险(同等) 仅仅是 3.3%(1.4%-4.3%) 。rs1410996 也与独立于 Y402H 的 AMD 被联系。为带一拷贝风险等位基因和二拷贝风险的个人的 exudative AMD 的 ORs 等位基因是 2.57 (1.21-5.45 ) 并且 4.76 (2.15-10.55 ) 分别地,与 28.3% 的通信员同等(2.0%-40.5%) 并且 38.2%(21.8%-45.4%) 。在 HTRA1 的 rs11200638 是为 AMD 的另一个易受影响的地点,风险同质接合体为 exudutive AMD 是显著地易受影响的(OR=3.98, 1.88-8.43 ) 与 38.9% 的同等(24.3%-45.8%) 。吸烟的教育地位和香烟也与 exudative AMD 有关。在为环境风险因素控制以后, CFH 和 HTRA1 SNP 独立地与 exudative AMD 被联系,与或 3.50 (1.45-8.45 ) 为在 Y402H 的 CT 遗传型, 3.34 (1.33-8.36 ) 为在 rs1410996 的 GG 遗传型并且 3.85 (1.58-9.42 ) 为在 rs11200638 的 AA 遗传型分别地。在基因和环境因素之间的相互作用分析证明 synergistically 吸烟为 rs1410996 的异质接合体增加了 AMD 的危险性,与 7.33 的 ORinteraction (Pinteraction=0.029 ) 。在一张汉中国人口, CFH 和 HTRA1 多型性的结论看起来是独立地并且可能 additivelv 世袭贡献者到 exudative AMD。Y402H 多型性与风险等位基因的低频率比在白种人用汉语授与重要却相对更低的贡献。基因环境相互作用可以是与高基因的风险鼓励那些由避免可修改的因素阻止 AMD 的一个最好的方法直到为 AMD 有有效处理。 | CHU Jie ZHOU Cheng-chao LU Ning ZHANG Xue DONG Fang-tian | 2008 | Chinese Medical Journal2008，，24： | 9 |
| 13 | Application of video-assisted thoracic surgery in the standard operation for thoracic tumors显示文摘Objective: To evaluate the short-term outcomes of video-assisted thoracic surgery (VATS) for thoracic tumors. Methods: The data of 1,790 consecutive patients were retrospectively reviewed. These patients underwent VATS pulmonary resections, VATS esophagectomies, and VATS resections of mediastinal tumors or biopsies at the Cancer Institute & Hospital, Chinese Academy of Medical Sciences between January 2009 and January 2012. Results: There were 33 patients converted to open thoracotomy (OT, 1.84%). The overall morbidity and mortality rate was 2.79% (50/1790) and 0.28% (5/1790), respectively. The overall hospitalization and chest tube duration were shorter in the VATS lobectomy group (n=949) than in the open thoracotomy (OT) lobectomy group (n=753). There were no significant differences in morbidity rate, mortality rate and operation time between the two groups. In the esophageal cancer patients, no significant difference was found in the number of nodal dissection, chest tube duration, morbidity rate, mortality rate, and hospital length of stay between the VATS esophagectomy group (n=81) and open esophagectomy group (n=81). However, the operation time was longer in the VATS esophagectomy group. In the thymoma patients, there was no significant difference in the chest tube duration, morbidity rate, mortality rate, and hospital length of stay between the VATS thymectomy group (n=41) and open thymectomy group (n=41). However, the operation time was longer in the VATS group. The median tumor size in the VATS thymectomy group was comparable with that in the OT group. Conclusions: In early-stage (I/II) non-small cell lung cancer patients who underwent lobectomies, VATS is comparable with the OT approach with similar short-term outcomes. In patients with resectable esophageal cancer, VATS esophagectomy is comparable with OT esophagectomy with similar morbidity and mortality. VATS thymectomy for Masaoka stage I and II thymoma is feasible and safe, and tumor size is not contraindicated. Longer follow-ups are needed to determine the oncologic equivalency of VATS lobectomy, esophagectomy, and thymectomy for thymoma vs. OT. | Ju-Wei Mu Gui-Yu Chen Ke-Lin Sun Da-Wei Wang Bai-Hua Zhang Ning Li Fang Lv You-Sheng Mao Qi Xue Shu-Geng Gao Jun Zhao Da-Li Wang Zhi-Shan Li Wen-Dong Lei Yu-Shun Gao Liangze Zhang Jin-Feng Huang Kang Shao Kai Su Kun Yang Liang Zhao Fei-Yue Feng Yong-Gang Wang Jian Li Jie He | 2013 | Cancer Biology & Medicine2013，10，1： | 8 |
| 14 | Efficacy of Lubeikangru formulation in mice with hyperplasia of the mammary glands induced by estrogen and progesterone显示文摘OBJECTIVE: To evaluate the protective effects of Lubeikangru formula(LF) on hyperplasia of the mammary glands(HMG) induced by estrogen and progesterone in mice.METHODS: Female mice were divided randomly into five groups: normal, model, tamoxifen(3 mg/kg),Rupixiao(900 mg/kg) and LF(900 mg/kg). All mice except those in the normal group were treated sequentially with estradiol and progesterone to induce HMG. From the tenth day of induction, mice in normal and model groups received distilled water and mice in the other groups were given the corresponding drugs by gavage, once a day, for 30 d.At the end of treatment, the mammary glands, ovaries, hypothalamus, and serum was collected for whole-mount and hematoxylin and eosin(HE)staining, enzyme-linked immunosorbent assays(ELISAs), or western blotting.RESULTS: Whole-mount and HE staining of mammary glands showed that LF rescued(at least in part) the hyperplasic morphology of the mammary glands, and the number of branch points decreased after LF treatment(P < 0.05). ELISAs revealed that levels of estrogen and progesterone were decreased following LF treatment, whereas levels of gonadotropin-releasing hormone, follicle-stimulating hormone, and luteinizing hormone were increased in serum and tissues. Western blotting confirmed that LF treatment led to a reduction in expression of phosphorylated(p)-Erk, p-p38 and p-c-Jun N-terminal kinase. LF was also confirmed to be safe by acute-toxicity tests.CONCLUSION: LF can protect the mammary glands of mice from estrogen-and progesterone-induced hyperplasia by adjusting hormone levels and regulating the mitogen-activated protein kinase pathway. | Sun Li Guo Donghui Liu Qian Xue Yanan Jiang Ning Zheng Hongxin | 2019 | Journal of Traditional Chinese Medicine2019，39，2： | 8 |
| 15 | Epidemiological survey of echinococcosis in Tibet Autonomous Region of China显示文摘Background:The echinococcosis is prevalent in 10 provinces/autonomous region in western and northern China.Epidemiological survey of echinococcosis in China in 2012 showed the average prevalence of four counties in Tibet Autonomous Region(TAR)is 4.23%,much higher than the average prevalence in China(0.24%).It is important to understand the transmission risks and the prevalence of echinococcosis in human and animals in TAR.Methods:A stratified and proportionate sampling method was used to select samples in TAR.The selected residents were examined by B-ultrasonography diagnostic,and the faeces of dogs were tested for the canine coproantigen against Echinococcus spp.using enzyme-linked immunosorbent assay.The internal organs of slaughtered domestic animals were examined by visual examination and palpation.The awareness of the prevention and control of echinococcosis among of residents and students was investigated using questionnaire.All data were inputted using double entry in the Epi Info database,with error correction by double-entry comparison,the statistical analysis of all data was processed using SPSS 21.0,and the map was mapped using ArcGIS 10.1,the data was tested by Chi-square test and Cochran-Armitage trend test.Results:A total of 80384 people,7564 faeces of dogs,and 2103 internal organs of slaughtered domestic animals were examined.The prevalence of echinococcosis in humans in TAR was 1.66%,the positive rate in females(1.92%)was significantly higher than that in males(1.41%),(X2=30.31,P<0.01),the positive rate of echinococcosis was positively associated with age(X2trend=-423.95,P<0.01),and the occupational populations with high positive rates of echinococcosis were herdsmen(3.66%)and monks(3.48%).The average positive rate of Echinococcus coproantigen in TAR was 7.30%.The positive rate of echinococcosis in livestock for the whole region was 11.84%.The average awareness rate of echinococcosis across the region was 33.39%.Conclusions:A high prevalence of echinococcosis is found across the TAR,representing a very serious concern to human health.Efforts should be made to develop an action plan for echinococcosis prevention and control as soon as possible,so as to control the endemic of echinococcosis and reduce the medical burden on the population. | Bin Li Gongsang Quzhen Chui-Zhao Xue Shuai Han Wei-Qi Chen Xin-Liu Yan Zhong-Jie Li MLinda Quick Yong Huang Ning Xiao Ying Wang Li-Ying Wang Gesang Zuoga Bianba Gangzhu Bing-Cheng Ma Gasong Xiao-Gang Wei Niji Can-Jun Zheng Wei-Ping Wu Xiao-Nong Zhou | 2019 | Infectious Diseases of Poverty2019，8，2： | 8 |
| 16 | Differential gene expression profiling of gastric intraepithelial neoplasia and early-stage adenocarcinoma显示文摘AIM:To investigate the differentiated whole genome expression profiling of gastric high-and low-grade intraepithelial neoplasia and early-stage adenocarcinoma.METHODS:Gastric specimens from an upper magnifying chromoendoscopic targeted biopsy were collected from March 2010 to May 2013.Whole genome expression profiling was performed on 19 low-grade intraepithelial neoplasia(LGIN),20 high-grade intraepithelial neoplasia(HGIN),19 early-stage adenocarcinoma(EGC),and 19 chronic gastritis tissue samples using Agilent 4×44K Whole Human Genome microarrays.Differentially expressed genes between different types of lesions were identified using an unpaired t-test and corrected with the Benjamini and Hochberg false discovery rate algorithm.A gene ontology(GO)enrichment analysis was performed using the Gene Spring software GX 12.6.The differentially expressed gene was verified using a real-time TaqManPCR assay with independent tissue samples,including 26 LGIN,15 HGIN,14 EGC,and 20 chronic gastritis.The expression of G0S2 were further validated by immunohistochemical staining(IHC)in 24 LGIN,40 HGIN,30 EGC and 61 chronic gastritis specimens.RESULTS:The gene expression patterns of LGIN and HGIN tissues were distinct.There were 2521 significantly differentially expressed transcripts in HGIN,with951 upregulated and 1570 downregulated.A GO enrichment analysis demonstrated that the most striking overexpressed transcripts in HGIN compared with LGIN were in the category of metabolism,defense response,and nuclear factorκB(NF-κB)cascade.While the vast majority of transcripts had barely altered expression in HGIN and EGC tissues,only 38 transcripts were upregulated in EGC.A GO enrichment analysis revealed that the alterations of the immune response were most prominent in the progression from HGIN to EGC.It is worth noting that,compared with LGIN,289 transcriptswere expressed at higher levels both in HGIN and EGC.A characteristic gene,G0/G1 switch 2(G0S2)was one of the 289 transcripts and related to metabolism,the immune response,and the NF-κB cascade,and its expression was validated in independent samples through real-time TaqManPCR and immunohistochemical staining.In real-time PCR analysis,the expression of G0S2 was elevated both in HGIN and EGC compared with that in LGIN(P<0.01 and P<0.001,respectively).In IHC analysis,G0S2 immunoreactivity was detected in the cytoplasmic of neoplastic cells,but was undetectable in chronic gastritis cells.The G0S2 expression in HGIN was higher than that of LGIN(P=0.012,χ2=6.28)and EGC(P=0.008,χ2=6.94).CONCLUSION:A clear biological distinction between gastric high-and low-grade intraepithelial neoplasia was identified,and provides molecular evidence for clinical application. | Xue Xu Lin Feng Yu Liu Wei-Xun Zhou Ying-Cai Ma Gui-Jun Fei Ning An Yuan Li Xi Wu Fang Yao Shu-Jun Cheng Xing-Hua Lu | 2014 | World Journal of Gastroenterology2014，20，47： | 8 |
| 17 | Impact of different termination modes on atrial fibrillation termination in catheter ablation of persistent atrial fibrillation显示文摘 | WANG Ping DONG Jian-zeng LONG De-yong NING Man TANG Ri-bo YU Rong-hui XUE Zeng-ming SANG Cai-hua JIANG Chen-xi MA Chang-sheng | 2012 | Chinese Medical Journal2012，，11： | 7 |
| 18 | Deformation features and failure mechanism of steep rock slope under the mining activities and rainfall显示文摘Underground mining activities and rainfall have potential important influence on the initiation and reactivation of the slope deformations,especially on the steep rock slope. In this paper,using the discrete element method(UDEC),numerical simulation was carried out to investigate deformation features and the failure mechanism of the steep rock slope under mining activities and rainfall. A steep rock slope numerical model was created based on a case study at the Wulong area in Chongqing city,China. Mechanical parameters of the rock mass have been determined by situ measurements and laboratory measurements. A preliminary site monitoring system has been realized,aiming at getting structure movements and stresses of unstablerock masses at the most significant discontinuities. According to the numerical model calibrated based on the monitoring data,four types of operation conditions are designed to reveal the effect of mining excavation and extreme rainfall on the deformation of the steep rock slope. | LI Zhi-qiang XUE Yi-guo LI Shu-cai ZHANG Le-wen WANG Dan LI Bin ZHANG Wen NING Kai ZHU Jian-ye | 2017 | Journal of Mountain Science2017，14，1： | 7 |
| 19 | Theoretical and numerical research of wire array Z-pinch and dynamic hohlraum at IAPCM显示文摘Dense Z-pinch plasmas are powerful and energy-efficient laboratory sources of X-rays,and show the possibility to drive inertial confinement fusion(ICF).Recent advances in wire-array Z-pinch and Z-pinch dynamic hohlraum(ZPDH)researches at the Institute of Applied Physics and Computational Mathematics are presented in this paper.Models are setup to study different physical processes.A full circuit model(FCM)was used to study the coupling between Z-pinch implosion and generator discharge.A mass injection model with azimuthal modulation was setup to simulate the wire-array plasma initiation,and the two-dimensional MHD code MARED was developed to investigate the Z-pinch implosion,MRT instability,stagnation and radiation.Implosions of nested and quasi-spherical wire arrays were also investigated theoretically and numerically.Key processes of ZPDH,such as the arrayefoam interaction,formation of the hohlraum radiation,as well as the following capsule ablation and implosion,were analyzed with different radiation magneto-hydrodynamics(RMHD)codes.An integrated 2D RMHD simulation of dynamic hohlraum driven capsule implosion provides us the physical insights of wire-array plasma acceleration,shock generation and propagation,hohlraum formation,radiation ablation,and fuel compression. | Ning Ding Yang Zhang Delong Xiao Jiming Wu Zihuan Dai Li Yin Zhiming Gao Shunkai Sun Chuang Xue Cheng Ning Xiaojian Shu Jianguo Wang | 2016 | Matter and Radiation at Extremes2016，1，3： | 7 |
| 20 | Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19: An evidence-based clinical practice guideline(updated version)显示文摘The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the cause of a rapidly spreading illness,coronavirus disease 2019(COVID-19),affecting more than seventeen million people around the world.Diagnosis and treatment guidelines for clinicians caring for patients are needed.In the early stage,we have issued'A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV)infected pneumonia(standard version)';now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline.We formed a working group of clinical experts and methodologists.The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas:chemoprophylaxis,diagnosis,treatments,and discharge management.We searched the literature for direct evidence on the management of COVID-19,and assessed its certainty generated recommendations using the Grading of Recommendations,Assessment,Development and Evaluation(GRADE)approach.Recommendations were either strong or weak,or in the form of ungraded consensus-based statement.Finally,we issued 34 statements.Among them,6 were strong recommendations for,14 were weak recommendations for,3 were weak recommendations against and 11 were ungraded consensus-based statement.They covered topics of chemoprophylaxis(including agents and Traditional Chinese Medicine(TCM)agents),diagnosis(including clinical manifestations,reverse transcription-polymerase chain reaction(RT-PCR),respiratory tract specimens,IgM and IgG antibody tests,chest computed tomography,chest X-ray,and CT features of asymptomatic infections),treatments(including lopinavirritonavir,umifenovir,favipiravir,interferon,remdesivir,combination of antiviral drugs,hydroxychloroquine/chloroquine,interleukin-6 inhibitors,interleukin-1 inhibitors,glucocorticoid,qingfei paidu decoction,lianhua qingwen granules/capsules,convalescent plasma,lung transplantation,invasive or noninvasive ventilation,and extracorporeal membrane oxygenation(ECMO)),and discharge management(including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge).We also created two figures of these recommendations for the implementation purpose.We hope these recommendations can help support healthcare workers caring for COVID-19 patients. | Ying-Hui Jin Qing-Yuan Zhan Zhi-Yong Peng Xue-Qun Ren Xun-Tao Yin Lin Cai Yu-Feng Yuan Ji-Rong Yue Xiao-Chun Zhang Qi-Wen Yang Jianguang Ji Jian Xia Yi-Rong Li Fu-Xiang Zhou Ya-Dong Gao Zhui Yu Feng Xu Ming-Li Tu Li-Ming Tan Min Yang Fang Chen Xiao-Ju Zhang Mei Zeng Yu Zhu Xin-Can Liu Jian Yang Dong-Chi Zhao Yu-Feng Ding Ning Hou Fu-Bing Wang Hao Chen Yong-Gang Zhang Wei Li Wen Chen Yue-Xian Shi Xiu-Zhi Yang Xue-Jun Wang Yan-Jun Zhong Ming-Juan Zhao Bing-Hui Li Lin-Lu Ma Hao Zi Na Wang Yun-Yun Wang Shao-Fu Yu Lu-Yao Li Qiao Huang Hong Weng Xiang-Ying Ren Li-Sha Luo Man-Ru Fan Di Huang Hong-Yang Xue Lin-Xin Yu Jin-Ping Gao Tong Deng Xian-Tao Zeng Hong-Jun Li Zhen-Shun Cheng Xiao-Mei Yao Xing-Huan Wang | 2020 | Military Medical Research2020，7，3： | 6 |
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