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20篇 您的检索式:作者名="Mo Sha"
    题名 作者 年代 出处 被引量
1Multi-Class Classification Methods of Cost-Conscious LS-SVM for Fault Diagnosis of Blast Furnace显示文摘Aiming at the limitations of rapid fault diagnosis of blast furnace,a novel strategy based on cost-conscious least squares support vector machine(LS-SVM)is proposed to solve this problem.Firstly,modified discrete particle swarm optimization is applied to optimize the feature selection and the LS-SVM parameters.Secondly,cost-conscious formula is presented for fitness function and it contains in detail training time,recognition accuracy and the feature selection.The CLS-SVM algorithm is presented to increase the performance of the LS-SVM classifier.The new method can select the best fault features in much shorter time and have fewer support vectors and better generalization performance in the application of fault diagnosis of the blast furnace.Thirdly,a gradual change binary tree is established for blast furnace faults diagnosis.It is a multi-class classification method based on center-of-gravity formula distance of cluster.A gradual change classification percentage is used to select sample randomly.The proposed new method raises the speed of diagnosis,optimizes the classification accuracy and has good generalization ability for fault diagnosis of the application of blast furnace.LIU Li-mei WANG An-na SHA Mo ZHAO Feng-yun 2011Journal of Iron and Steel Research(International)2011,18,10:13
2A New Process Industry Fault Diagnosis Algorithm Based on Ensemble Improved Binary-Tree SVM显示文摘Support vector machine(SVM) is an effective tool in deal with small sample, nonlinear and high dimension classification problems. In this paper, an improved pre-treatment binary-tree SVM is proposed to solve fault diagnosis. Furthermore an ensemble method is presented to establish ensemble SVM. Here the improved SVM is used as weak learning machine. The new ensemble SVM can improve the performance of single binary-tree SVM.At the end, the new algorithm is applied to fault diagnosis of blast furnace faults and the Tennessee Eastman process(TEP). The experiments results show that the improved binary-tree SVM algorithm has an excellent performance on diagnosis speed and accuracy.WANG Anna SHA Mo LIU Limei CHU Maoxiang 2015Chinese Journal of Electronics2015,24,2:5
3Enhancement of oral bioavailability and anti-Parkinsonian efficacy of resveratrol through a nanocrystal formulation显示文摘Resveratrol(RES),a non-flavonoid polyphenol extracted from a wide variety of plants,exhibits neuroprotective activities against Parkinson’s disease(PD).However,undesirable water solubility of RES reduces its oral bioavailability and demonstrates lowefficacy in blood and brain,thus limiting its application.In present study,a nanocrystal formulation of RES(RES-NCs)was developed to enhance its oral bioavailability and delivery into brain for PD treatment.RES-NCs were fabricated with hydroxypropyl methylcellulose(HPMC)stabilizer via antisolvent precipitation approach.The obtained RES-NCs displayed the particle size of 222.54±1.66 nm,the PDI of 0.125±0.035,the zeta potential of−9.41±0.37mV,and a rapid in vitro dissolution rate.Molecular dynamics simulation of RES and HPMC revealed an interaction energy of−68.09 kJ/mol and a binding energy of−30.98±0.388 kJ/mol,indicating that the spontaneous binding between the two molecules is through van der Waals forces.RES-NCs conferred enhanced cellular uptake as well as improved permeability relative to pure RES.In addition,RES-NCs were able to protect neurons against cytotoxicity induced by MPP+.Meanwhile,RES-NCs exerted no significant toxic effects on zebrafish embryos and larvae,and did not influence their survival and hatching rates.When orally administered to rats,RES-NCs exhibited more favorable pharmacokinetics than pure RES,with higher plasma and brain concentrations.More importantly,MPTP-induced PD mice showed notable improvements in behavior,attenuated dopamine deficiency,and elevated levels of dopamine and its metabolites after the treatment with RES-NCs.Furthermore,immunoblot analysis revealed that the neuroprotective role of RES-NCsmay be at least partially mediated by Akt/Gsk3βsignaling pathway.Taken altogether,RES-NCs can serve as a potential treatment modality for PD,offering means of improving RES oral bioavailability and brain accumulation.Sha Xiong Wei Liu Yile Zhou Yousheng Mo Yao Liu Xiaojia Chen Huafeng Pan Dongsheng Yuan QiWang Tongkai Chen 2020Asian Journal of Pharmaceutical Sciences2020,15,4:3
4Anode materials for potassium-ion batteries: Current status and prospects显示文摘Potassium-ion batteries(KIBs)as one of the most promising alternatives to lithium-ion batteries have been highly valued in recent years.However,progress in KIBs is largely restricted by the sluggish development in anode materials.Therefore,it is imperative to systematically outline and evaluate the recent research advances in the field of anode materials for KIBs toward promoting the development of high-performance anode materials for KIBs.In this review,the recent achievements in anode materials for KIBs are summarized.The electrochemical properties(ie.charge storage mechanism,capacity,rate performance,and cycling stability)of these reported anode materials,as well as their advantages/disadvantages,are discerned and analyzed,enabling high-performance KIBs to meet the requirements for practical applications.Finally,technological developments,scientific challenges,and future research opportunities of anode materials for KIBs are briefly reviewed.Mo Sha Long Liu Huaping Zhao Yong Lei 2020Carbon Energy2020,2,3:3
5Multi-class Classification Methods of Enhanced LS-TWSVM for Strip Steel Surface Defects显示文摘Considering strip steel surface defect samples,a multi-class classification method was proposed based on enhanced least squares twin support vector machines(ELS-TWSVMs)and binary tree.Firstly,pruning region samples center method with adjustable pruning scale was used to prune data samples.This method could reduce classifier′s training time and testing time.Secondly,ELS-TWSVM was proposed to classify the data samples.By introducing error variable contribution parameter and weight parameter,ELS-TWSVM could restrain the impact of noise samples and have better classification accuracy.Finally,multi-class classification algorithms of ELS-TWSVM were proposed by combining ELS-TWSVM and complete binary tree.Some experiments were made on two-dimensional datasets and strip steel surface defect datasets.The experiments showed that the multi-class classification methods of ELS-TWSVM had higher classification speed and accuracy for the datasets with large-scale,unbalanced and noise samples.Mao-xiang CHU An-na WANG Rong-fen GONG Mo SHA 2014Journal of Iron and Steel Research(International)2014,21,2:3
6Highly-rough surface carbon nanofibers film as an effective interlayer for lithium–sulfur batteries显示文摘Lithium–sulfur(Li–S)battery with a new configuration is demonstrated by inserting a flexible nitrogen-doping carbon nanofiber(N-CNFs)interlayer between the sulfur cathode and the separator.The N-CNFs film with high surface roughness and surface area is fabricated by electrospinning and a subsequent calcination process.The N-CNFs film interlayer not only effectively traps the shuttling migration of polysulfides but also gives the whole battery reliable electronic conductivity,which can effectively enhance the electrochemical performance of Li–S batteries.Finally,Li–S batteries with long cycling stability of 785 mAh/g after 200 cycles and good rate capability of 573 mAh/g at 5 C are achieved.Hongfan Zhu Mo Sha Huaping Zhao Yuting Nie Xuhui Sun Yong Lei 2020Journal of Semiconductors2020,41,9:3
7A distal femoral supra-condylar plate: biomechanical comparison with condylar plate and first clinical application for treatment of supracondylar fracture显示文摘Bowei Liang Zhenqi Ding Junguo Shen Wenliang Zhai Liangqi Kang Liang Zhou Mo Sha Dongzhu Liang 2012International Orthopaedics2012,,8:1
8A vibrational model of open celled polyurethane foam automotive seat cushions显示文摘PATTEN W N SHA S MO C 1998Journal of Sound and Vibration1998,217,1:1
9An Energy Optimization Protocol Based on Cross-Layer for Wireless Sensor Networks 显示文摘Bai Yuebin Liu Shujuan Sha Mo 2008Journal of Communications2008,3,6:1
10Rapid detection of aflatoxin B 1 on membrane by dot-immunogold filtration assay显示文摘Yang Ye Youxiang Zhou Zhenzhen Mo Wei Cheng Sha Yang Xiaohong Wang Fusheng Chen 2010Talanta2010,,3:1
11High thermoelectric performance at room temperature of n-type Mg_(3)Bi_(2)-based materials by Se doping显示文摘Bi_(2)Te_(3) based alloys have been the most widely used thermoelectric material at low temperature for many decades.Here we report Se doped n-type Mg_(3)Bi_(2) based materials with a thermoelectric figure-of-merit ZT of 0.82 at 300 K and a peak ZT of 1.24 at 498 K,which is comparable to the n-type Bi_(2)Te_(3) and Te doped Mg_(3)Bi_(1.4)Sb_(0.6).The improved thermoelectric performance is benefited from the high carrier concentration and mobility as well as the thermal conductivity reduction.The reduced resistivity increased the power factor at all measured temperatures,leading to a higher engineering ZT(ZTeng)and engineering power factor(PFeng)for n-type Mg_(3)Bi_(2).The n-type Mg_(3)Bi_(1.4)Sb_(0.6) materials are promising for thermoelectric power generation and cooling applications near room temperature.Xiaobo Mo Jiansong Liao Guocai Yuan Sha Zhu Xiaobo Lei Lihong Huang Qinyong Zhang Chao Wang Zhifeng Ren 2022Journal of Magnesium and Alloys2022,10,4:1
12A vibration model of opencelled polyurethane foam automotive seat cushions显示文摘Patten W N Sha S Mo C 1998Journal of Sound and Vibration1998,217,1:1
13The potential for biochar application in rubber plantations in Xishuangbanna,Southwest China:a pot trial显示文摘Biochar can enhance crop production and sequester carbon,but there have been few studies with tree crops.Rubber plantations cover more than 8 million hectares in Southeast Asia,so we assessed the feasibility of biochar application in these plantations with a pot trial.Rubber seedlings were planted in soil with four concentrations(0,1.25%,2.5% and 5%,w/w)of biochar combined with two concentrations of compound fertilizer(0 kg/ha and 300 kg/ha).Soil properties and seedling growth were measured,and a leaching experiment was conducted in the rainy season.Our results show that biochar increased pH,water content(27.4-65.1%),total carbon(25.4-53.6%),nitrate nitrogen,and available phosphorus in the soil,and decreased bulk density(3.2-23.9%).Biochar treatment reduced leaching of ammonium nitrogen and ortho-P.Biochar increased seedling nutrient uptake(C,N,P and K),with 2.5% and 5% biochar showing the largest effects,but seedling biomass was the highest with 1.25%,and declined in 2.5% and 5%.Our results suggest that biochar addition is an effective way to improve rubber plantation soils,sequester more carbon and decrease nutrient leaching,but the optimum application rate under field conditions needs further research.Libing Pan Fanzhen Xu Huizhu Mo Richard T.Corlett Liqing Sha 2021Biochar2021,3,1:0
14A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue显示文摘Due to the abuse of antibiotics,antibiotic residues can be detected in both natural environment and various industrial products,posing threat to the environment and human health.Here we describe the design and implementation of an engineered Escherichia coli capable of degrading tetracycline(Tc)-one of the commonly used antibiotics once on humans and now on poultry,cattle and fisheries.A Tcdegrading enzyme,TetX,from the obligate anaerobe Bacteroides fragilis was cloned and recombinantly expressed in E.coli and fully characterized,including its Km and kcat value.We quantitatively evaluated its activity both in vitro and in vivo by UVeVis spectrometer and LC-MS.Moreover,we used a tetracycline inducible amplification circuit including T7 RNA polymerase and its specific promoter PT7 to enhance the expression level of TetX,and studied the dose-response of TetX under different inducer concentrations.Since the deployment of genetically modified organisms(GMOs)outside laboratory brings about safety concerns,it is necessary to explore the possibility of integrating a kill-switch.Toxin-Antitoxin(TA)systems were used to construct a mutually dependent host-plasmid platform and biocontainment systems in various academic and industrious situations.We selected nine TA systems from various bacteria strains and measured the toxicity of toxins(T)and the detoxifying activity of cognate antitoxins(A)to validate their potential to be used to build a kill-switch.These results prove the possibility of using engineered microorganisms to tackle antibiotic residues in environment efficiently and safely.Zepeng Mu Zhuoning Zou Ye Yang Wenbo Wang Yue Xu Jianyi Huang Ruiling Cai Ye Liu Yajin Mo Boyi Wang Yiqun Dang Yongming Li Yushan Liu Yueren Jiang Qingyang Tan Xiaohong Liu Cheng Hu Hua Li Sha Wei Chunbo Lou Yang Yu Jiangyun Wang 2018Synthetic and Systems Biotechnology2018,3,3:0
15Detection of Endocrine Disruptors in Water around Landfills显示文摘[Objectives]This study was conducted to explore the occurrence levels of endocrine disruptors(EDCs)in rural areas around a county landfill in Tongren City.[Methods]The water around the landfill was sampled and analyzed.A solid-phase extraction and high performance liquid chromatography-tandem mass spectrometry(SPE-UPLC-MS/MS)method was established for the determination of 27 EDCs.After the HLB solid-phase extraction column was activated,a water sample,which was adjusted with phosphoric acid to a pH of 2(±0.5)and added with 500 mg of disodium EDTA,was loaded,and 5 ml of water and 20%methanol water was added for washing.Next,10 ml of elution solution was added for elution,and the collected eluate was evaporated under reduced pressure at 40℃to near dryness,and 1 ml of reconstitution solution was added to a constant volume.An ACQUITY UPLC BEH C18(100×2.1 mm,2.6μm)chromatographic column was adopted for LC separation by gradient elution with pure water solution-acetonitrile as the mobile phase.For MS detection,the MRM mode was adopted for collection,and the positive and negative ion modes were switched for simultaneous determination,and the internal standard method was used for quantification.[Results]The correlation coefficient R2 was greater than 0.99 in the linear range of each target substance.The limits of quantitation in the method were between 0.05 and 2.00 ng/L,and the recoveries ranged from 75.3%to 105.7%.[Conclusions]The method has high sensitivity,good accuracy and strong practical value.Dandan LIU Qing LI Yaohong LIU Sha FENG Tao MO Zheng LIU Xiaonan ZOU 2024Agricultural Biotechnology2024,13,1:0
16Deep electrical structures of Qinzhou-Fangcheng Junction Zone in Guangxi and seismogenic environment of the 1936 Lingshan M6^(3/4)earthquake显示文摘The tectonic position of the southwest section of the Qinzhou Bay-Hangzhou Bay Tectonic Junction Zone(QHTJZ)can be determined by examining the Qinzhou-Fangcheng Junction Zone(QFJZ)in Guangxi.This zone is significant because it was the location of the largest earthquake ever recorded in the inland region of South China,specifically the 1936 Lingshan M6^(3/4)earthquake in Guangxi.Therefore,this region serves as an optimal location for researching the origins of intraplate earthquakes in South China.This study presents a display of a broadband magnetotelluric(MT)prospecting profile that traverses the Guangxi QFJZ and the Lingshan earthquake zone,extending from the northwest(NW)to the southeast(SE).A resistivity structure model was generated using three-dimensional(3D)inversion technology along the profile.The main faults in QFJZ were analyzed in terms of their deep extension forms and tectonic attributes.This analysis was performed by integrating the results obtained from geology,gravity,wave velocity ratio,Global Position System(GPS),and geothermal flow.The results showed that(1)the Dongzhong-Xiaodong fault(DXf),the eastern Fangcheng-Lingshan fault(FLf2),and the eastern Hepu-Beiliu fault(HBf2)were all trans-crustal deep faults,and crust-mantle ductile shear zones developed in the deep part.Two electrical boundary zones,DXf and HBf2,were identified.DXf inclined towards the northwest,while HBf2 inclined towards the southeast.The FangchengLingshan fault(FLf)exhibits a tectonic style resembling a“flower”shape in the upper crust.In the deeper section,it is characterized by an electrical boundary zone that gradually slopes towards the southeast direction.(2)The Hunan-Guangxi Passive Continental Margin(HGPCM)on the NW side of DXf had a stratified resistivity structure and relatively stable Bouguer gravity anomalies,which conformed to the quasi-craton tectonic attribute of the local failure at the southeastern margin of the Yangtze Block(YB).The southeastern side of this block is marked by the presence of the QFJZ and Yunkai Magmatic Arc(YKMA).These areas exhibit varying Bouguer gravity anomalies,indicating a combination of high and low resistivity in their electrical structures.This suggests that this zone has undergone multiple stages of structural evolution and transformation.The giant high-resistivity body under the Qinzhou-Fangcheng Remnant Ocean Basin(QFROB)might be the trace left by the extinction of the South China Ocean and the collision orogeny between YB and the Cathaysian Block(CB).The presence of sublow-resistivity layers in the middle-lower crust between the Liuwandashan Magmatic Arc(LMA)and YKMA indicates that this particular zone is being influenced from a distance by magmatic activities originating from the Leiqiong mantle.(3)The focal area of the 1936 Lingshan earthquake was located in the brittle high-resistivity body with a low strain rate.Under the coupling action of NWW-SEE regional tectonic stress and deep thermodynamic force,the brittle high-resistivity body in the upper crust became the main body for accumulating the tectonic stress.The Lingshan earthquake occurred due to the dextral strike-slip fracture instability of FLf2,a rock layer with slightly lower strength in the sub-high-resistivity zone.This instability was triggered when the accumulated stress reached the ultimate rock strength.The unveiling of the seismogenic model of the Lingshan earthquake,as presented in this study,holds significant scientific importance in comprehending the factors contributing to intraplate earthquakes in the South China region.Bin ZHOU Chunheng YAN Yan ZHAN Xiangyu SUN Sha LI Xiang WEN Yajun MO Yongdong YUAN Yuan YUAN Meili HUANG 2024Science China Earth Sciences2024,67,2:0
17Effects of iodoacetic acid drinking water disinfection byproduct on the gut microbiota and its metabolism in rats显示文摘Iodoacetic acid(IAA) is an unregulated disinfection byproduct in drinking water and has been shown to exert cytotoxicity, genotoxicity, tumorigenicity, and reproductive and developmental toxicity. However, the effects of IAA on gut microbiota and its metabolism are still unknown, especially the association between gut microbiota and the metabolism and toxicity of IAA. In this study, female and male Sprague–Dawley rats were exposed to IAA at 0 and 16 mg/kg bw/day daily for 8 weeks by oral gavage. Results of 16S r RNA gene sequencing showed that IAA could alter the diversity, relative abundance and function of gut microbiota in female and male rats. IAA also increased the abundance of genes related to steroid hormone biosynthesis in the gut microbiota of male rats. Moreover, metabolomics profiling revealed that IAA could significantly disturb 6 and 13 metabolites in the feces of female and male rats, respectively. In female rats, the level of androstanediol increased in the IAA treatment group. These results were consistent with our previous findings, where IAA was identified as an androgen disruptor. Additionally, the perturbed gut microbiota and altered metabolites were correlated with each other. The results of this study indicated that IAA could disturb gut microbiota and its metabolism. These changes in gut microbiota and its metabolism were associated with the reproductive and developmental toxicity of IAA.Yujie Sha Huan Wu Yue Guo Xi Liu Yan Mo Qiyuan Yang Shumao Wei Kunling Long Du Lu Ying Xia Weiwei Zheng Zhiheng Su Xiao Wei 2022Journal of Environmental Sciences2022,34,7:0
18Rotationplasty type BIIIb as an effective alternative to limb salvage procedure in adults:Two case reports显示文摘BACKGROUND Rotationplasty is often performed for malignant tumors,but type BIIIb rotationplasty is rarely reported,and there needs to be more evidence of the procedure and treatment.The purpose of this case study was to report a new direction in the use of type BIIIb rotationplasty in treating patients with limb salvage and longterm non-healing infections.CASE SUMMARY Case 1:A 47-year-old man underwent radiotherapy for hemangioendothelioma in his left thigh,resulting in a femoral fracture.Despite the use of plates,intramedullary nailing,and external fixators,the femoral bone failed to unite due to infectious nonunion.Multiple operations were unable to control the infection,leaving the patient immobile.We performed a modified tibia-pelvic-constrained hip rotationplasty,utilizing a constrained prosthetic hip between the tibia and pelvis following a femur resection.Two years post-surgery,the patient was able to walk with the prosthetic device without any signs of recurring infection.The corresponding functional scores were 72 points for the Musculoskeletal Tumor Society(MSTS),53 for the Functional Mobility Assessment(FMA),93 for the Toronto Extremity Salvage Score(TESS),and 56 for the MOS 36-item short form health survey(SF-36).Case 2:A 59-year-old woman presented with liposarcoma in her left thigh.Magnetic resonance imaging revealed tumors in the medial,anterior,and posterior femur muscles,encircling the femoral vessels and nerves.Fortunately,there were no symptoms of sciatic dysfunction,and the tumor had not invaded the sciatic nucleus.After one year of follow-up,the patient expressed satisfaction with limb preservation post-type BIIIb rotationplasty.The corresponding functional scores were 63 points for the MSTS,47 for the FMA,88 for the TESS,and 52 for the SF-36.CONCLUSION Our study suggests that type BIIIb rotationplasty may be an alternative to amputation in patients with incurable infections.For malignant tumors of the lower extremities without invasion of the sciatic nerve,type BIIIb rotationplasty remains an excellent alternative to amputation.This surgical method may prevent amputation,improve functional outcomes,and facilitate biological reconstruction.Zhang-Xin Chen Xiao-Wei Guo Hai-Sen Hong Cong Zhang Wei Xie Mo Sha Zhen-Qi Ding 2023World Journal of Clinical Cases2023,11,28:0
19全基因组关联研究发现中国人群前列腺癌两个新易感位点9q31.2和19q13.4显示文摘0前言在全球范围内,前列腺癌的发病率和病死率存在着巨大差异。该病在西方发达国家发病率最高,在非裔美国人群病死率最高,而在亚洲人群中发病率及病死率均为全球最低,提示不同人种在前列腺癌的遗传方面存在异质性。在欧美和日本人群中,全基因组关联研究(GWAS)技术已经被用于检测前列腺癌的遗传易感性位点,但至今尚无关于GWAS检测中国人群前列腺癌易感位点的报道。Jianfeng Xu Zengnan Mo Dingwei Ye Meilin Wang Fang Liu Guangfu Jin Chuanliang Xu Xiang Wang Qiang Shao Zhiwen Chen Zhihua Tao Jun Qi Fangjian Zhou Zhong Wang Yaowen Fu Dalin He Qiang Wei Jianming Guo Denglong Wu Xin Gao Jianlin Yuan Gongxian Wang Yong Xu Guozeng Wang Haijun Yao Pei Dong Yang Jiao Mo Shen Jin Yang Jun Ou-Yang Haowen Jiang Yao Zhu Shancheng Ren Zhengdong Zhang Changjun Yin Xu Gao Bo Dai Zhibin Hu Yajun Yang Qijun Wu Hongyan Chen Peng Peng Ying Zheng Xiaodong Zheng Yongbing Xiang Jirong Long Jian Gong Rong Na Xiaoling Lin Hongjie Yu Sha Tao Junjie Feng Jishan Sun Wennuan Liu Ann Hsing Jianyu Rao Qiang Ding Fredirik Wiklund Henrik Gronberg Xiao-Ou Shu Wei Zheng Hongbing Shen Li Jin Rong Shi Daru Lu Xuejun Zhang Jielin Sun S Lilly Zheng Yinghao Sun 2013第二军医大学学报2013,34,4:0
20Mesoporous Cu_(3−x)Zn_(x)(BTC)_(2)nanocubes synthesized in deep eutectic solvent and their catalytic performances显示文摘To develop high-performance metal-organic frameworks(MOFs)for catalysis is of great importance.Here,we synthesized the mesoporous Cu_(3−x)Zn_(x)(BTC)_(2)(BTC=benzene-1,3,5-tricarboxylate)nanocubes in a deep eutectic solvent of ZnCl_(2)/ethylene glycol solution.The route can proceed at room temperature and the reaction time needed is shortened to be 30 min,which is superior to the conventional solvothermal route that usually needs high temperature and long reaction time.The formation mechanism of the mesoporous Cu_(3−x)Zn_(x)(BTC)_(2)nanocubes in deep eutectic solvent(DES)was investigated by in situ synchrotron X-ray diffraction/small angle X-ray scattering/X-ray absorption fine structure conjunction technique.The mesoporous Cu_(3−x)Zn_(x)(BTC)_(2)nanocubes exhibit high catalytic activity and reusability for cyanosilylation reaction of benzaldehyde and aerobic oxidation reaction of benzylic alcohol.Jingyang Hu Jianling Zhang Xiuniang Tan Xiuyan Cheng Zhuizhui Su Lixiong Qian Mingzhao Xu Yufei Sha Yanyue Wang Yisen Yang Yunpeng Liu Guang Mo Xueqing Xing Zhonghua Wu 2023Nano Research2023,16,3:0
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