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| 1 | 评估大气细颗粒物暴露引起的全球疾病负担的一个综合风险函数显示文摘[背景]评估由于环境空气中细颗粒物(PM2.5)长期暴露引起的疾病负担,需要了解相对风险度(RR)函数的图形和幅度。然而,在世界上很多观察到高PM2.5环境浓度的地方,都缺乏足够的直接证据确定死亡RR函数的图形。[目的]建立可用于全球PM2.5暴露范围内成人死因的RR函数:缺血性心脏病(IHD)、脑血管疾病(脑卒中)、慢性阻塞性肺病(COPD)和肺癌(LC)。建立急性下呼吸道感染(ALRI)发病的RR函数,用于估算〈5岁的儿童中死亡率和健康生活损失年数。[方法]通过整合现有的来自于环境空气污染(AAP)、二手烟草烟雾、家用固体烹饪用燃料和主动吸烟(AS)等研究的RR信息,拟合综合暴露-反应(IER)模型。采用颗粒物质的吸入剂量,通过转化AS暴露来估计全年PM2.5的暴露当量。在估算全球环境PM2.5浓度的基础上,得出各国的人群归因分数(PAFs)。[结果]与以往负担评估中使用的其他7种模型相比,IER模型是一种优越的RR预测方法。在不同国家中,归因于AAP暴露的PAF百分数各不相同,分别为:IHD,2~41;脑卒中,1~43;COPD,〈1~21;LC,〈1~25;ALRI,〈1~38。[结论]我们建立了一种以细颗粒物为基础的RR模型,它通过整合来自不同类型的燃烧所产生颗粒物排放的RR信息,涵盖全球范围的暴露。当获得新的RR信息时,可以对这个模型进行更新。 | Richard T.Burnett C.Arden Pope III Majid Ezzati Casey Olives Stephen S.Lim Sumi Mehta Hwashin H.Shin Gitanjali Singh Bryan Hubbell Michael Brauer H.Ross Anderson Kirk R.Smith John R.Balmes Nigel G.Bruce Haidong Kan Francine Laden Annette Prüss-Ustün Michelle C.Turner Susan M.Gapstur W.Ryan Diver Aaron Cohen 何蓉 张伊人 金泰廙 | 2014 | 环境与职业医学2014，31，11： | 80 |
| 2 | 婴幼儿卒中的处理:来自美国心脏协会卒中委员会和青少年心血管病委员会专门写作组的科学声明显示文摘目的:本声明旨在回顾有关儿童卒中的文献,为最佳的诊断和治疗提供推荐意见,供负责诊断和治疗婴儿、儿童和青少年脑血管病的医生使用。方法:写作组成员由美国心脏协会卒中委员会科学声明监督委员会任命,由多个不同领域的专家组成。应用美国心脏协会卒中委员会的证据分级标准对每条推荐意见进行分级。在经过小组成员审查后,草稿由4位同行评议专家和卒中委员会指导委员会成员审阅,并由美国心脏协会科学咨询和协调委员会批准。我们预计该声明需在4年内进行更新。结果:为镰状细胞病、烟雾病、颈一脑动脉夹层分离和心源性栓塞引起的缺血性卒中的预防提供循证推荐,同时为出血性卒中的评价和处理提供推荐意见。提出儿童使用肝素和华法林的剂量方案。另外,还为围产期卒中和儿童脑静脉窦血栓形成的评价和处理提供推荐。 | E.Steve Roach(著) Meredith R. Golomb(著) Robert Adams(著) Jose Biller(著) Stephen Daniels(著) Gabrielle deVeber(著) Donna Ferriero(著) Blaise V. Jones(著) Fenella J. Kirkham(著) R. Michael Scott(著) Edward R. Smith(著) 刘丽芳(译) 王胜男(译) 王伟(译) 林镇洲(译) 朱佳佳(译) 潘速跃(译) | 2008 | 国际脑血管病杂志2008，16，11： | 60 |
| 3 | Prevalence of Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis Among a Largely Middle-Aged Population Utilizing Ultrasound and Liver Biopsy: A Prospective Study显示文摘 | Christopher D. Williams Joel Stengel Michael I. Asike Dawn M. Torres Janet Shaw Maricela Contreras Cristy L. Landt Stephen A. Harrison | 2011 | Gastroenterology2011，，1： | 20 |
| 4 | The impact of iodinated X-ray contrast agents on formation and toxicity of disinfection by-products in drinking water显示文摘The presence of iodinated X-ray contrast media(ICM) in source waters is of high concern to public health because of their potential to generate highly toxic disinfection by-products(DBPs). The objective of this study was to determine the impact of ICM in source waters and the type of disinfectant on the overall toxicity of DBP mixtures and to determine which ICM and reaction conditions give rise to toxic by-products. Source waters collected from Akron,OH were treated with five different ICMs, including iopamidol, iopromide, iohexol,diatrizoate and iomeprol, with or without chlorine or chloramine disinfection. The reaction product mixtures were concentrated with XAD resins and the mammalian cell cytotoxicity and genotoxicity of the reaction mixture concentrates was measured. Water containing iopamidol generated an enhanced level of mammalian cell cytotoxicity and genotoxicity after disinfection. While chlorine disinfection with iopamidol resulted in the highest cytotoxicity overall, the relative iopamidol-mediated increase in toxicity was greater when chloramine was used as the disinfectant compared with chlorine. Four other ICMs(iopromide, iohexol, diatrizoate, and iomeprol) expressed some cytotoxicity over the control without any disinfection, and induced higher cytotoxicity when chlorinated. Only iohexol enhanced genotoxicity compared to the chlorinated source water. | Clara H.Jeong Edward J.Machek Morteza Shakeri Stephen E.Duirk Thomas A.Ternes Susan D.Richardson Elizabeth D.Wagner Michael J.Plewa | 2017 | Journal of Environmental Sciences2017，29，8： | 11 |
| 5 | Shale Gas Well Completions and Maximizing Gas Recoveries显示文摘It is shown that stress fields within the earth are the principle control for hydraulic fracture direction in horizontal shale gas wells. Hydraulic fracturing is a process of increasing permeability within gas shales and involves a sophisticated organization of technology, good planning and proper management of equipment over a very short time period to be successful. The direction and extent of the induced fractures can be determined in near real-time at the well site via application of earthquake seismology theory in a now common process known as frac mapping. Next to the horizontal lateral azimuth, the total volume of slurry pumped into the well is a major factor in determining well EURs. Vertical fracture growth can be controlled and is important in concentration of the slurry within the main zone target zone that has the high TOC and porosity. Cemented casing with perforations is currently the most used method for zone isolation. New open-hole sleeve packers may eventually provide more flexibility in fracture design while also providing a means for refracturing multi-stage fractured horizontal wells, a technique not now commonly available. Multi-Stage fracture design requires incorporating rock properties with fracturing effect simulations and then verifying results using 3D reservoir simulations. Maximizing the gas recovery factors and EURs can be accomplished through use of closely spaced laterals with inter-fingered fracture stages and exploiting the stress shadow fracturing phenomenon. Even greater EURs may be possible if the wells can be refractured thereby opening up additional permeability channels. Shale gas development has progressed in an environmentally sensitive manner within the U.S. and will continue in this manner. During the past ten years, all of these technologies have been either newly developed or were the advancement of existing technology with modifications. The opportunity exists to take these proven technologies to other areas of the world for exploitation of shale gas reservoirs. | Michael D. Burnaman Stephen Smith Wenwu Xia | 2009 | 中国石油勘探2009，14，3： | 8 |
| 6 | 肝上腔静脉成形术联合肝后腔静脉扩展术在肝移植中的应用:首次115例经验报道显示文摘目的:本文介绍原位肝移植中腔静脉成形术的方法并评估其结果。方法:1994年11月至2000年9月,我们对连续115例原位肝移植(包括6例左外侧叶及2例右叶移植)病人采用了腔静脉成形术。 在起初的4年里,53例(66.3%)移植病人需行股-腋静脉体外转流,而以后的2年内该比例降至8例(22.9%)。无一例病人需中转为背驮式或经典术式。结果:中位手术时间4.5h,中位热缺血时间25min,中位输血量6U浓缩红细胞。上述结果在首次移植及再次移植间无差异。本组无与腔静脉成形技术相关的围手术期死亡。包括活体原位肝移植在内,均未观察到肝静脉流出道梗阻。没有病人因急性肾功能衰竭而需术后血透。病人留置于ICU的中位天数2d,中位住院天数为10d。结论:腔静脉成形术无需解剖肝后腔静脉、肝静脉或肝短静脉,肝下腔静脉也得以保留,并因此更有利于肝切除及维持稳定的血流动力学,在再次移植中优势更为明显。大开口的三角形腔静脉吻合容易操作,能在较短时间内完成供肝植入,且易与吻合血管口径的匹配,并可避免流出道阻塞。操作时间的缩短减少了静脉体外转流的可能。我们认为腔静脉成形技术能适用于所有病人,且相应手术的并发症少。 | You Min Wu Michael Voigt Stephen Rayhili Daniel Kartz Rou-Yee Chenhsu Warren Schmidt Rachel Miller Frank Mtros Douglas Labrecque 周光文 | 2002 | 外科理论与实践2002，7，2： | 7 |
| 7 | Joint Motion Control of a Powered Lower Limb Orthosis for Rehabilitation显示文摘许多病人与针伤害被限制到轮椅,在 carer 上与第二等的病理和增加的依赖导致一种坐着的生活方式。增加的证据证明了运动训练减少这些第二等的病理的发生,但是物理努力在这涉及训练是以便有差的依从。这篇论文在一个新鈥渉u 人的设计和控制上报导友好鈥 ? orthosis (皮骨骼) ,以高力量灵魂肌肉致动器(pMAs ) 为动力。一个高度顺从的使活动系统的联合与臀部,膝,和脚关节放感觉的聪明的嵌入的控制机制,速度,加速和力量,生产强大还为半身不遂者病人的内在地安全的操作。这份报纸在零装载下面分析脚关节,膝,和新潮的关节的运动,并且装载哪个模仿人的手足质量,证明鈥渟o 英尺鈥 ? 致动器的使用能提供一个光滑的用户友好运动。这种技术的申请将极大地为半身不遂者病人改进使复权协议。关键词皮骨骼 - 集中人的机器人学 - 人的适应 Mechatronics - 灵魂肌肉致动器(pMAs )- 康复尼尔森肋前缘在计算机并且在 1999 的在机器的系统和自动化的在 1995 的电子设计和他的 M.Sc 收到了他的 B.Sc 度,在 Coimbra 的大学,葡萄牙。他当前在 Salford 的大学在先进机器人学正在追求博士,更大的英国曼彻斯特。他的研究兴趣包括传感器,致动器,电源来源,力量扩大皮骨骼,康复机器人学和触觉的反馈系统。米兰 Bezdicek 在 2003 在捷克的共和国从 Brno 工业大学在 Mechatronics 收到了他的 M.Sc。他当前在 Salford 的大学是机器人学和 haptics 的博士学生,英国。他的研究兴趣包括 biomechanics,电子学,计算机控制了分析的用机器制造的、人工的神经网络, haptics, DSP,聪明的传感器和结构。约翰· O ·格雷在控制工程从大学曼彻斯特获得了他的博士和 D.Sc 学位,他的早工作在非线性的控制系统, C.A.D 过程和精确的方面上集中了电磁的乐器学。在 1988,与学术、工业的同事一起,他建立了英国国家先进机器人学研究中心。在 1990 年代,他最近在先进机器人学和更多上组织了一系列 EC.sponsored 会议(2001 鈥 ? 005 ) 先进机器人学上的主持 EPSRC 和 IEE 网络。Darwin G. 考德威尔分别地在 1986 和 1990 从赫尔的大学在机器人学收到了他的 B.Sc 和博士。在 1994 在里面在管理收到了 M.Sc。自从 1999,他在 Salford 的大学是在机器人学和自动化的中心的先进机器人学的主席。他是超过 150 篇学术论文的作者或合作者并且有 4 项专利。他的研究兴趣包括为食物工业的创新致动器和传感器,触觉的反馈,力量扩大皮骨骼,灵巧的操纵者,类人动物机器人学, biomimetic 系统,康复机器人学和机器的系统。考德威尔教授当前是 IEEE (机器人学和自动化社会) 的 UKRI 区域的主席。史蒂芬·胡钦斯与 HPC 在 1978 作为 orthotist 毕业了是在在 Salford 和登记 orthotist 的大学的 Orthotics 的高级讲师。他为在预防的膝 bracing 要过去常的原型 orthotic 膝关节的设计在 1997 由研究在 Orthotics 获得了 M.Sc。他当前在断断续续的跛的处理在摇椅脚底开发的题目上正在追求博士。他在 orthotic 膝关节设计上有很多项英国专利。他的研究兴趣包括更低的手足 orthotics 和鞋类设计的所有方面。 | Nelson Costa Milan Bezdicek Michael Brown John O.Gray Darwin G.Caldwell Stephen Hutchins | 2006 | International Journal of Automation and computing2006，3，3： | 7 |
| 8 | Next-Generation Stool DNA Test Accurately Detects Colorectal Cancer and Large Adenomas显示文摘 | David A. Ahlquist Hongzhi Zou Michael Domanico Douglas W. Mahoney Tracy C. Yab William R. Taylor Malinda L. Butz Stephen N. Thibodeau Linda Rabeneck Lawrence F. Paszat Kenneth W. Kinzler Bert Vogelstein Niels Chr. Bjerregaard S?ren Laurberg Henrik Toft S? | 2012 | Gastroenterology2012，，2： | 7 |
| 9 | Computed tomography and patient risk:Facts,perceptions and uncertainties显示文摘Since its introduction in the 1970s, computed tomography(CT) has revolutionized diagnostic decision-making. One of the major concerns associated with the widespread use of CT is the associated increased radiation exposure incurred by patients. The link between ionizing radiation and the subsequent development of neoplasia has been largely based on extrapolating data from studies of survivors of the atomic bombs dropped in Japan in 1945 and on assessments of the increased relative risk of neoplasia in those occupationally exposed to radiation within the nuclear industry. However, the association between exposure to low-dose radiation from diagnostic imaging examinations and oncogenesis remains unclear. With improved technology, significant advances have already been achieved with regards to radiation dose reduction. There are several dose optimization strategies available that may be readily employed including omitting unnecessary images at the ends of acquired series, minimizing the number of phases acquired, and the use of automated exposure control as opposed to fixed tube current techniques. In addition, new image reconstruction techniques that reduce radiation dose have been developed in recent years with promising results. These techniques use iterative reconstruction algorithms to attain diagnostic quality images with reduced image noise at lower radiation doses. | Stephen P Power Fiachra Moloney Maria Twomey Karl James Owen J O'Connor Michael M Maher | 2016 | World Journal of Radiology2016，8，12： | 7 |
| 10 | Failed biliary cannulation: Clinical and technical outcomes after tertiary referral endoscopic retrograde cholangiopancreatography显示文摘AIM: Prospective evaluation of repeat endoscopic retrograde cholangiopancreatography (ERCP) for failed Schutz grade 1 biliary cannulation in a high-volume center. METHODS: Prospective intention-to-treat analysis of patients referred for biliary cannulation following recent unsuccessful ERCP. RESULTS: Fifty-one patients (35 female; mean age: 62.5 years; age range: 40-87 years) with previous failed biliary cannulation were referred for repeat ERCP. The indication for ERCP was primarily choledocholithiasis (45%) or pancreatic malignancy (18%). Successful biliary can- nulation was 100%. The precut needle knife sphincterotomy (NKS) rate was 27.4%. Complications occurred in 3.9% (post-ERCP pancreatitis). An identif iable reason for initial unsuccessful biliary cannulation was present in 55% of cases. Compared to a cohort of 940 nave pa-pilla patients (female 61%; mean age: 59.9 years; age range: 18-94 years) who required sphincterotomy over the same time period, there was no statistical difference in the cannulation success rate (100% vs 98%) or postERCP pancreatitis (3.1% vs 3.9%). Precut NKS use was more frequent (27.4% vs 12.7%) (P = 0.017). CONCLUSION: Referral to a high-volume center following unsuccessful ERCP is associated with high technical success, with a favorable complication rate, compared to routine ERCP procedures. | Michael P Swan Michael J Bourke Stephen J Williams Sina Alexander Alan Moss Rick Hope David Ruppin | 2011 | World Journal of Gastroenterology2011，17，45： | 6 |
| 11 | 循证设计策略在医疗建筑环境领域的应用研究显示文摘文章分析了循证设计原理及三要素,回顾了其发展历程,从自然元素、空间环境和功能设备3个方面详细阐述了循证设计策略的开发模式,旨在进一步推动循证设计在我国医疗建筑设计中的应用,促进整体医疗环境的优化发展。 | 班淇超 陈冰 Stephen Sharples Michael Phiri | 2016 | 中国医院建筑与装备2016，17，10： | 6 |
| 12 | 纸条检测法对第85窟壁画空鼓灌浆脱盐效果的评估显示文摘实验得出不损伤壁画的纸条检测法与壁画表面盐分含量的关系,并以此为根据,采用纸条法测定壁画表面盐分的含量状态,评价壁画空鼓灌浆后脱盐的效果。结果表明壁画空鼓灌浆所带入的水分激活了地仗中的盐分,使盐分运移并大量地富集到壁画的表面。保护笔的脱盐效果十分显著,纸条法测得保护笔脱盐后Cl-浓度降低约10个ppm,相当于保护笔脱盐可以减少了壁画表面地仗中所含1.6%的Cl-的量,即可以去除掉未灌浆区域壁画表面盐分的80%。实验证明纸条法可以应用于壁画脱盐效果的检验。 | 陈港泉 Michael Schilling 李燕飞 Neville Agnew Joy Keeney Stephen Rickerby 于宗仁 Lisa Shekede | 2007 | 敦煌研究2007，，5： | 5 |
| 13 | 红曲对中度血脂升高美国人调节血脂作用的临床研究显示文摘[目的]评价在饮食控制的情况下红曲(RYRCholestin)对美国高血脂人群的降脂作用和安全性。[方法]开放标记,自身对照,多中心临床试验。187名美国高血脂人群入选(血清低密度脂蛋白胆固醇3.50—4.92mmol/L,总胆固醇5.18—7.25mmol/L;男116人,女71人),162入完成试验。在整个试验中受试者遵照美国国家胆固醇教育计划的饮食要求。试验的前4周仅控制饮食,第5周至第12周连续服用8周RYRCholestin(每日2.4g)。[结果]经前4周饮食控制,血清脂质各指标无显著变化。口服RYRCholestin8周后,血清总胆固醇、低密度脂蛋白胆固醇和三酰甘油分别降低16.6%,24.O%和25.2%,高密度脂蛋白胆固醇升高14.3%(P〈0.001)。显效率为79.0%,有效率为97.5%。停服14d(2周)后,血脂各指标回到试验前水平。试验中共发现可能与服用产品相关的、轻度的29项不良反应。[结论]RYRCholestin的耐受性良好,它可降低血清总胆固醇、低密度脂蛋白胆固醇和三酰甘油,升高血清高密度脂蛋白胆固醇。停服2用后受试者的血脂各项指标均恢复到试验前水平。 | 陆继红 Kermit Bonovich Harry Colfer Michael Davidson Carlos A.Dujovne David L.Fried Mitchell Greenspan Ronald P.Karlsberg Stephen P.King Craig F.LaForce Marc Litt J.Robert McGhee 曾润海 朱佳石 谈宁芝 | 2013 | 上海预防医学2013，25，9： | 5 |
| 14 | Prevalence of Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis Among a Largely Middle-Aged Population Utilizing Ultrasound and Liver Biopsy: A Prospective Study显示文摘 | Christopher D. Williams Joel Stengel Michael I. Asike Dawn M. Torres Janet Shaw Maricela Contreras Cristy L. Landt Stephen A. Harrison | 2011 | Gastroenterology2011，，1： | 5 |
| 15 | Quality of Accounting Information and Internal Audit Characteristics in Nigeria显示文摘 | Oyebisi Ogundana Stephen Ojeka Michael Ojua Chukwu Nwaze | 2017 | Journal of Modern Accounting and Auditing2017，13，8： | 4 |
| 16 | Quality indicators for colonoscopy显示文摘 | Douglas K. Rex John L. Petrini Todd H. Baron Amitabh Chak Jonathan Cohen Stephen E. Deal Brenda Hoffman Brian C. Jacobson Klaus Mergener Bret T. Petersen Michael A. Safdi Douglas O. Faigel Irving M. Pike | 2006 | Gastrointestinal Endoscopy2006，，4： | 4 |
| 17 | Impact of chlorine exposure time on disinfection byproduct formation in the presence of iopamidol and natural organic matter during chloramination显示文摘Chloramines,in practice,are formed onsite by adding ammonia to chlorinated drinking water to achieve the required disinfection.While regulated disinfection byproducts(DBPs)are reduced during chloramine disinfection,other DBPs such as iodinated(iodo-)DBPs,that elicit greater toxicity are formed.The objective of this study was to investigate the impact of prechlorination time on the formation of both halogen-specific total organic halogen(TOX)and iodo/chlorinated(chloro-)DBPs during prechlorination/chloramination in source waters(SWs)containing iopamidol,an X-ray contrast medium.Barberton SW(BSW)and Cleveland SW(CSW)containing iopamidol were prechlorinated for 5–60 min and afterwards chloraminated for 72 hr with ammonium chloride.Chlorine contact time(CCT)did not significantly impact total organic iodine(TOI)concentrations after prechlorination or chloramination.Concentrations of total organic chlorine(TOCl)formed during prechlorination did not significantly change regardless of pH and prechlorination time,whileTOClappearedtodecreaseafter 72 hrchloraminationperiod.Dichloroiodomethane(CHCl_2I)formation during prechlorination did not exhibit any significant trends as a function of p H or CCT,but after chloramination,significant increases were observed at pHs 6.5 and 7.5 with respect to CCT.Iodo-HAAs were not formed during prechlorination but were detected after chloramination.Significant quantities of chloroform(CHCl_3)and trichloroacetic acid(TCAA)were formed during prechlorination but formation ceased upon ammonia addition.Therefore,prechlorination studies should measure TOX and DBP concentrations prior to ammonia addition to obtain data regarding the initial conditions. | Nana Osei B.Ackerson Alexis H.Killinger Hannah K.Liberatore Thomas A.Ternes Michael J.Plewa Susan D.Richardson Stephen E.Duirk | 2019 | Journal of Environmental Sciences2019，31，4： | 4 |
| 18 | The Stool DNA Test Is More Accurate Than the Plasma Septin 9 Test in Detecting Colorectal Neoplasia显示文摘 | David A. Ahlquist William R. Taylor Douglas W. Mahoney Hongzhi Zou Michael Domanico Stephen N. Thibodeau Lisa A. Boardman Barry M. Berger Graham P. Lidgard | 2012 | Clinical Gastroenterology and Hepatology2012，，3： | 4 |
| 19 | Campylobacter concisus and inflammatory bowel disease显示文摘Investigation of the possible role of Campylobacter concisus(C. concisus) in inflammatory bowel disease(IBD) is an emerging research area. Despite the association found between C. concisus and IBD, it has been difficult to explain how C. concisus, a bacterium that is commonly present in the human oral cavity, may contribute to the development of enteric diseases. The evidence presented in this review shows that some C. concisus strains in the oral cavity acquired zonula occludens toxin(zot) gene from a virus(prophage) and that C. concisus Zot shares conserved motifs with both Vibrio cholerae Zot receptor binding domain and human zonulin receptor binding domain. Both Vibrio cholerae Zot and human zonulin are known to increase intestinal permeability by affecting the tight junctions. Increased intestinal permeability is a feature of IBD. Based on these data, we propose that a primary barrier function defect caused by C. concisus Zot is a mechanism by which zot-positive C. concisus strains may trigger the onset and relapse of IBD. | Li Zhang Hoyul Lee Michael C Grimm Stephen M Riordan Andrew S Day Daniel A Lemberg | 2014 | World Journal of Gastroenterology2014，20，5： | 4 |
| 20 | Reference quality assembly of the 3.5-Gb genome of Capsicum annuum from a single linked-read library显示文摘Linked-Read sequencing technology has recently been employed successfully for de novo assembly of human genomes,however,the utility of this technology for complex plant genomes is unproven.We evaluated the technology for this purpose by sequencing the 3.5-gigabase(Gb)diploid pepper(Capsicum annuum)genome with a single Linked-Read library.Plant genomes,including pepper,are characterized by long,highly similar repetitive sequences.Accordingly,significant effort is used to ensure that the sequenced plant is highly homozygous and the resulting assembly is a haploid consensus.With a phased assembly approach,we targeted a heterozygous F1 derived from a wide cross to assess the ability to derive both haplotypes and characterize a pungency gene with a large insertion/deletion.The Supernova software generated a highly ordered,more contiguous sequence assembly than all currently available C.annuum reference genomes.Over 83%of the final assembly was anchored and oriented using four publicly available de novo linkage maps.A comparison of the annotation of conserved eukaryotic genes indicated the completeness of assembly.The validity of the phased assembly is further demonstrated with the complete recovery of both 2.5-Kb insertion/deletion haplotypes of the PUN1 locus in the F1 sample that represents pungent and nonpungent peppers,as well as nearly full recovery of the BUSCO2 gene set within each of the two haplotypes.The most contiguous pepper genome assembly to date has been generated which demonstrates that Linked-Read library technology provides a tool to de novo assemble complex highly repetitive heterozygous plant genomes.This technology can provide an opportunity to cost-effectively develop high-quality genome assemblies for other complex plants and compare structural and gene differences through accurate haplotype reconstruction. | Amanda M.Hulse-Kemp Shamoni Maheshwari Kevin Stoffel Theresa A.Hill David Jaffe Stephen R.Williams Neil Weisenfeld Srividya Ramakrishnan Vijay Kumar Preyas Shah Michael C.Schatz Deanna M.Church Allen Van Deynze | 2018 | Horticulture Research2018，5，1： | 4 |
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