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| 1 | CRISPR/Cas9:A powerful tool for crop genome editing显示文摘The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and represents a new generation of targeted genome editing technology that can be applied to nearly all organisms. Site-specific modification is achieved by a single guide RNA(usually about 20nucleotides) that is complementary to a target gene or locus and is anchored by a protospaceradjacent motif. Cas9 nuclease then cleaves the targeted DNA to generate double-strand breaks(DSBs), which are subsequently repaired by non-homologous end joining(NHEJ) or homology-directed repair(HDR) mechanisms. NHEJ may introduce indels that cause frame shift mutations and hence the disruption of gene functions. When combined with double or multiplex guide RNA design, NHEJ may also introduce targeted chromosome deletions,whereas HDR can be engineered for target gene correction, gene replacement, and gene knock-in. In this review, we briefly survey the history of the CRISPR/Cas9 system invention and its genome-editing mechanism. We also describe the most recent innovation of the CRISPR/Cas9 technology, particularly the broad applications of modified Cas9 variants, and discuss the potential of this system for targeted genome editing and modification for crop improvement. | Gaoyuan Song Meiling Jia Kai Chen Xingchen Kong Bushra Khattak Chuanxiao Xie Aili Li Long Mao | 2016 | The Crop Journal2016，4，2： | 4 |
| 2 | Effect of Salinity on the Survival, Ions and Urea Modulation in Red-eared Slider(Trachemys scripta elegans)显示文摘To understand the tolerance to salinity and osmoregulation of the introduced Trachemys scripta elegans, the salinity stress of four groups(salinity 5‰, 15‰, 25‰ and control group) were conducted. Inorganic ions, osmotic pressure, glucose and aldosterone of blood and urine in T. s. elegans(BW: 125.60 ± 19.84 g) were analyzed at 30 d, 60 d and 90 d stress. The results showed that: 1) inorganic ions concentration of blood and urine increased with ambient salinity, which indicated that high influx of ions was combined with higher outflow when exposed to saline water in T. s. elegans. However, blood aldosterone decreased with increasing salinity, which indicated that an increased sodium intake resulting in a diminished aldosterone production. However, with elapsed time, inorganic ions in urine decreased, which indicated that inorganic ions in blood would be accumulated, and Na+ and Cl– in the plasma inevitably build up to harmful levels, at last death was happening when T. s. elegans was exposed to salinity 25 during 90 d salinity stress; 2) blood osmotic pressure increased as ambient salinity increased, it would reach 400 mOsm/kg in the group of salinity 25, which was about 1.5 fold of the control group. Higher blood osmotic pressure was due to both higher blood ions and urea concentrations. There may be another mechanism to avoid an excess of NaCl together with an important loss of water using one of the end-products of nitrogen metabolism; 3) blood glucose in each group except the group of salinity 5 decreased with time elapsed and with salinity increased. Therefore, we can conclude that T. s. elegans is an osmoregulator that limits the entry of Na+ and Cl–, but can also tolerate certain degrees of increases in plasma Na+ and Cl–. When ambient salinity was lower than 15‰, T. s. elegans can increase blood osmotic pressure by balancing the entry of NaCl with the secretion of aldosterone decreased, and by accumulating blood urea for osmoregulation effectors, and survive for at least three months. These results could provide theoretical basis for salinity tolerance and the invasion on physiological mechanism for T. s. elegans. | Meiling HONG Ke ZHANG Chaohua SHU Di XIE Haitao SHI | 2014 | Asian Herpetological Research2014，5，2： | 2 |
| 3 | Monthly change of community structure of zoobenthos in Xiangxi Bay after impoundment of the Three Gorges Reservoir,China显示文摘Xiangxi Bay,a typical bay of the Three Gorges Reservoir(TGR)in China,was formed in June 2003 after the impoundment of the TGR.We investigated the community structure of zoobenthos in Xiangxi Bay from August 2003 to August 2004.Benthic abundance and biomass were calculated per unit area of sediment.A total of 26 taxa were identified.They included two families of Lamellibranchia(7.7%of the total taxa number),two families of Oligochaeta(50%),Chironomidae(38.5%),and one other animal(3.8%).The average density and biomass were 276 ind./m2 and 0.301 g/m2,respectively.Oligochaetes and chironomids constituted the major groups,and Limnodrilus hoffmeisteri,Procladius sp.,Branchiura sowerbyi,Tubificidae sp1.and Polypedilum scalaenum group sp.were the common taxa in this reservoir system.Among them,L.hoffmeisteri and Procladius sp.were the dominant species,and contributed 37.0%and 28.3%of the total density,respectively.The standing crop of zoobenthos in Xiangxi Bay was relative low,compared with other similar reservoirs.Our studies demonstrate that the standing crop increased gradually with time.The total density and biomass in August 2004 were 8.8 and 14.3 times those of the initial impoundment,separately.Oligochaetes did not become the sole dominant group,indicating that the sediment loading in Xiangxi Bay may be not serious. | SHAO Meiling XIE Zhicai YE Lin CAI Qinghua | 2007 | Frontiers in Biology2007，2，2： | 1 |
| 4 | Electrochemical quartz crystal microbalance study on growth and property of the polymer deposit at gold electrodes during oxidation of dopamine in aqueous solutions显示文摘 | Yunlong Li Meiling Liu Canhui Xiang Qingji Xie Shouzhuo Yao | 2005 | Thin Solid Films2005，，1： | 1 |
| 5 | Immunoglobu-lin A nephropathy in horseshoe kidney: case reports and litera-ture review显示文摘 | Panpan Hu Meiling Jin Yuansheng Xie | 2014 | Nephrology2014，19，10： | 1 |
| 6 | Improved refractory wound healing with administration of acidic fibroblast growth factor in diabetic rats显示文摘 | Liyun Xie Meiling Zhang Baijun Dong Mimi Guan Meifei Lu Zhifeng Huang Hongchang Gao Xiaokun Li | 2011 | Diabetes Research and Clinical Practice2011，，： | 1 |
| 7 | Electrochemical quartz crystal microbalance study on growth and property of the polymer deposit at gold electrodes during oxidation of dopamine in aqueous solutions显示文摘 | Yunlong Li Meiling Liu Canhui Xiang Qingji Xie Shouzhuo Yao | 2005 | Thin Solid Films2005，，1： | 1 |
| 8 | Improved refractory wound healing with administration of acidic fi- broblast growth factor in diabetic rats 显示文摘 | Xie Liyun Zhang Meiling Dong Baijun | 2011 | Diabetes Res Clin Pract2011，93，3： | 1 |
| 9 | Improved refractory wound healing with administration of acidic fibroblast growth factor in diabetic rats显示文摘 | Liyun Xie Meiling Zhang Baijun Dong | | 0，，03： | 1 |
| 10 | Review of Action Mechanisms of Acupuncture on Post-Traumatic Stress Disorder显示文摘Post-traumatic stress disorder(PTSD)is a kind of mental disorder caused by severe traumatic events.It has a high incidence,a serious of impacts on the physical and mental health of patients.Especially in the current situation of COVID-19,the researches on PTSD are particularly important,but the choice of drugs available for PTSD is limited and it is often accompanied by adverse reactions.In the field of acupuncture,there are many clinical research evidences suggested that PTSD is a predominant disease of acupuncture.However,its action mechanisms have not been fully elucidated,so the possible mechanisms of acupuncture in treating PTSD were discussed. | Chang LIU Ziman HE Xian WANG Lubao FENG Meiling ZHANG Yiqiang XIE Kai LI Mi LI | 2022 | Medicinal Plant2022，13，1： | 0 |
| 11 | Field distribution of the Z_(2)topological edge state revealed by cathodoluminescence nanoscopy显示文摘Photonic topological insulators with robust boundary states can enable great applications for optical communication and quantum emission,such as unidirectional waveguide and single-mode laser.However,because of the diffraction limit of light,the physical insight of topological resonance remains unexplored in detail,like the dark line that exists with the crys-talline symmetry-protected topological edge state.Here,we experimentally observe the dark line of the Z_(2)photonic topo-logical insulator in the visible range by photoluminescence and specify its location by cathodoluminescence characteriza-tion,and elucidate its mechanism with the p-d orbital electromagnetic field distribution which calculated by numerical sim-ulation.Our investigation provides a deeper understanding of Z_(2)topological edge states and may have great signific-ance to the design of future on-chip topological devices. | Xiao He Donglin Liu Hongfei Wang Liheng Zheng Bo Xu Biye Xie Meiling Jiang Zhixin Liu Jin Zhang Minghui Lu Zheyu Fang | 2022 | Opto-Electronic Advances2022，5，4： | 0 |
| 12 | Connectivity of fish assemblages along the mangrove-seagrasscoral reef continuum in Wenchang, China显示文摘Understanding the connectivity of fish among different typical habitats is important for conducting ecosystembased management, particularly when designing marine protected areas(MPA) or setting MPA networks. To clarify of connectivity among mangrove, seagrass beds, and coral reef habitats in Wenchang, Hainan Province,China, the fish community structure was studied in wet and dry seasons of 2018. Gill nets were placed across the three habitat types, and the number of species, individuals, and body size of individual fish were recorded. In total, 3 815 individuals belonging to 154 species of 57 families were collected. The highest number of individuals and species was documented in mangroves(117 species, 2 623 individuals), followed by coral reefs(61 species,438 individuals) and seagrass beds(46 species, 754 individuals). The similarity tests revealed highly significant differences among the three habitats. Approximately 23.4% species used two habitats and 11.0% species used three habitats. A significant difference(p<0.05) in habitat use among eight species(Mugil cephalus, Gerres oblongus, Siganus fuscescens, Terapon jarbua, Sillago maculata, Upeneus tragula, Lutjanus russellii, and Monacanthus chinensis) was detected, with a clear ontogenetic shift in habitat use from mangrove or seagrass beds to coral reefs. The similarity indices suggested that fish assemblages can be divided into three large groups namely coral, seagrass, and mangrove habitat types. This study demonstrated that connectivity exists between mangrove–seagrass–coral reef continuum in Wenchang area;therefore, we recommend that fish connectivity should be considered when designing MPAs or MPA network where possible. | Jianguo Du Meiling Xie Yuyu Wang Zehao Chen Wenhua Liu Jianji Liao Bin Chen | 2020 | Acta Oceanologica Sinica2020，39，8： | 0 |
| 13 | Targeting cancer cell plasticity by HDAC inhibition to reverse EBV-induced dedifferentiation in nasopharyngeal carcinoma显示文摘Application of differentiation therapy targeting cellular plasticity for the treatment of solid malignancies has been lagging.Nasopharyngeal carci noma(NPC)is a distinctive cancer with poor differe ntiatio n and high prevalenee of Epstein-Barr virus(EBV)infection.Here,we show that the expressi on of EBV latent protein LMP1 in duces dediffere ntiated and stem-like status with high plasticity through the transcriptional inhibition of CEBPA.Mechanistically,LMP1 upregulates STAT5A and recruits HDAC 1/2 to the CEBPA locus to reduce its histone acetylation.HDAC inhibition restored CEBPA expression,reversing cellular dedifferentiation and stem-like status in mouse xeno graft models.These fin dings provide a novel mecha nistic epigenetic-based in sight into virus-induced cellular plasticity and propose a promising concept of differentiation therapy in solid tumor by using HDAC inhibitors to target cellular plasticity. | Jiajun Xie Zifeng Wang Wenjun Fan Youping Liu Fang Liu Xiangbo Wan Meiling Liu Xuan Wang Deshun Zeng Van Wang Bin He Min Yan Zijian Zhang Mengjuan Zhang Zhijie Hou Chunli Wang Zhijie Kang Wenfeng Fang Li Zhang Eric W-F Lam Xiang Guo Jinsong Yan Yixin Zeng Mingyuan Chen Quentin Liu | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 0 |
| 14 | Metatranscriptomic analysis of host response and vaginal microbiome of patients with severe COVID-19显示文摘Dear Editor,The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)has become a global public health threat.Here we use a TRACE-seq-based metatranscriptomic analysis to compare host responses and vaginal microbiome of postmenopausal female patients with underlying severe COVID-19 disease with those of healthy females,thereby providing insights into the changes in the microenvironment of women's reproductive system. | Meng Xiao Bo Lu Rui Ding Xia Liu Xian Wu Yaqian Li Xudong Liu Lin Qiu Zhibo Zhang Jing Xie Yu Chen Dong Zhang Liting Dong Meiling Zhang Jinying Peng Hua Yang Timothy Kudihna Yingchun Xu Taisheng Li Chengqi Yi Lan Zhu | 2022 | Science China(Life Sciences)2022，65，7： | 0 |
| 15 | Distribution Characteristics and Health Risk Assessment of Antimony in Atmospheric Particulates in a Northern City of China显示文摘Over the last several decades,scientists have established a wealth of evidence to demonstrate the risks posed to human health by toxic elements in atmospheric particulate matter(PM).Antimony(Sb),as one of ever ignored PM-bound heavy metals,attracts more and more attentions and has been regarded as one emerging air pollutant with the change of pollution sources of particulate matter.To study the distribution of Sb in PM with different particle sizes is of great practical significance for understanding its source and health risks.In this study,the size distributions of Sb in PM(PM_(2.5),PM_(10)and TSP)in different seasons were studied from July 2018 to May 2019.The high concentration of PM-bound Sb was found and the health risk was evaluated.Sb was enriched in fine particles and showed higher values in winter,which was probably caused by coal combustion and meteorological conditions.It was also found that traffic-related non-exhaust emissions might become another main contribution to fine particle Sb.Health risk assessment demonstrated that the hazard quotient(HQ)of Sb in PM_(2.5)and PM_(10)for children(PM_(2.5):1.54,PM_(10):1.32)exceeded the acceptable threshold and ingestion contributed the most to the HQ for both children and adults. | SHEN Yiwen ZHAO Hao ZHAO Changxian DONG Shuofei CAO Yuanming XIE Jiaojao LYU Meiling YUAN Chungang | 2023 | Chemical Research in Chinese Universities2023，39，3： | 0 |
| 16 | A piezoelectric spectro- electrochemical study on the structural interconversion for poly(o-phenylenediamine) between its ladder structure with phenazine units and polyaniline-like chains显示文摘The combination of reflectance UV-Vis spec- troelectrochemistry with electrochemical quartz crystal microbalance (EQCM) and separate reflectance FTIR char- acterization were used to investigate the structural intercon- version for poly(o-phenylenediamine) (PoPD) between its ladder structure with phenazine units and polyaniline-like linear chains. The poly(o-phenylenediamine) films potentio- statically (0.8 V vs. SCE) grew on Au electrodes from 0.20 mol·L?1 H2SO4 (PoPD1) or 0.40 mol·L?1 NaOH (PoPD2) aque- ous solution containing 0.20 mol·L?1 Na2SO4 + 0.10 mol·L?1 o-phenylenediamine. By considering the mass of deposited PoPD2 film obtained from the EQCM data and the charge consumed under the current peak at ca. 0.6 V vs. SCE for oxidation of -NH2 groups in as-prepared PoPD2 during po- tential cycling in 0.10 mol·L-1 aqueous H2SO4, the molar per- centage of the polyaniline-like chains was estimated to be 19% (relative to total phenylenediamine units), being in agreement with the result obtained from a formaldehyde- combination experiment through the aminocarbonyl reaction. After 40-cycle potential sweeps between 0.2 and 0.8 V vs. SCE the polyaniline-like chains in PoPD2 could be com- pletely converted via intramolecular cyclization into the lad- der structure with phenazine units. However, PoPD1 was found to be perfectly composed of the ladder structure with phenazine units, and after 40-cycle potential sweeps between ?0.4 and 0.1 V vs. SCE only 2.5% in molar percentage of PoPD’s ladder structure could be converted into polyani line-like chains, suggesting that the ladder structure with phenazine units is thermodynamically more stable due to its possessing higher conjugation. | TU Xinman XIE Qingji WANG Meiling ZHANG Youyu YAO Shouzhuo | 2005 | Chinese Science Bulletin2005，50，15： | 0 |
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