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| 1 | Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction显示文摘AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QTAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction.RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×107 and 1.67×107 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.L4×105 and 3.02×105 copies of HBV DNA per mL in the semen.CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. | Wei-Ping Qian Yue-Qiu Tan Ying Chen Ying Peng Zhi Li Guang-Xiu Lu Made C. Liu Hsiang-Fu Kung Ming-Ling He Li-Ka Shing | 2005 | World Journal of Gastroenterology2005，11，34： | 25 |
| 2 | Sorafenib inhibits growth and metastasis of hepatocellular carcinoma by blocking STAT3显示文摘AIM: To investigate the inhibitory role and the underlying mechanisms of sorafenib on signal transducer and activator of transcription 3 (STAT3) activity in hepatocellular carcinoma (HCC).METHODS: Human and rat HCC cell lines were treated with sorafenib. Proliferation and STAT3 dephosphorylation were assessed. Potential molecular mechanisms of STAT3 pathway inhibition by sorafenib were evaluated. In vivo antitumor action and STAT3 inhibition were investigated in an immunocompetent orthotopic rat HCC model.RESULTS: Sorafenib decreased STAT3 phosphorylationat the tyrosine and serine residues (Y705 and S727), but did not affect Janus kinase 2 (JAK2) and phosphatase shatterproof 2 (SHP2), which is associated with growth inhibition in HCC cells. Dephosphorylation of S727 was associated with attenuated extracellular signal-regulated kinase (ERK) phosphorylation, similar to the effects of a mitogen-activated protein kinase (MEK) inhibitor U0126, suggesting that sorafenib induced S727 dephosphorylation by inhibiting MEK/ERK signaling. Meanwhile, sorafenib could also inhibit Akt phosphorylation, and both the phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002 and Akt knockdown resulted in Y705 dephosphorylation, indicating that Y705 dephosphorylation by sorafenib was mediated by inhibiting the PI3K/Akt pathway. Finally, in the rat HCC model, sorafenib signifi cantly inhibited STAT3 activity, reducing tumor growth and metastasis.CONCLUSION: Sorafenib inhibits growth and metastasis of HCC in part by blocking the MEK/ERK/STAT3 and PI3K/Akt/STAT3 signaling pathways, but independent of JAK2 and SHP2 activation. | Fang-Ming Gu, Quan-Lin Li, Qiang Gao, Jia-Hao Jiang, Xiao-Yong Huang, Jin-Feng Pan, Jia Fan, Jian ZhouFang-Ming Gu, Quan-Lin Li, Qiang Gao, Jia-Hao Jiang, Xiao-Yong Huang, Jin-Feng Pan, Jia Fan, Jian Zhou, Liver Cancer Institute, Zhongshan Hospital and Shanghai Medical School, Fudan University, Shanghai 200032, China Author contributions: Gu FM, Li QL and Gao Q contributed equally to this work Gu FM and Li QL performed the experi- ments and interpretation of the data and statistical analysis Zhou J and Gao Q contributed to the conception and design of the study Gu FM, Gao Q, Li QL and Zhou J wrote the manuscript Jiang JH, Huang XY, Pan JF and Fan J made substantial contri- bution to the design and conception of the study and interpreta- tion of data all authors read and approved the f inal manuscript. | 2011 | World Journal of Gastroenterology2011，17，34： | 18 |
| 3 | Interleukin-6 and ratio of plasma interleukin-6/interleukin-10 as risk factors of symptomatic lumbar osteoarthritis显示文摘AIM To determine the role of cartilage oligomeric matrix protein(COMP), interleukin(IL)-6, IL-10 and ratio of IL-6/IL-10 as risk factors of symptomatic lumbar osteoarthritis(OA) in postmenopausal women with estrogen deficiency.METHODS Case-control study had been conducted in Sanglah General Hospital from October 2015 until March 2016. The blood samples were obtained and analyzed by enzyme-linked immunosorbent assay(ELISA).RESULTS From 44 pairs of samples which divided into 44 samples as case group and 44 samples as control group showed that high level of COMP in estrogen deficiency postmenopausal women were not at risk(OR = 0.7; 95%CI: 0.261-1.751; P = 0.393) for symptomatic lumbar OA(cut-off point 0.946). Estrogen deficiency in postmenopausal women with the high level of IL-6 had 2.7 times risk(OR = 2.7; 95%CI: 0.991-8.320; P = 0.033) for symptomatic lumbar OA from the low level of IL-6(cut-off point 2.264). At lower level of IL-10, there was no risk for symptomatic lumbar OA(OR = 0.6; 95%CI: 0.209-1.798; P = 0.345) than with the higher level of IL-10(cut-off point 6.049). While the high ratio of IL-6/IL-10 level in estrogen deficiency postmenopausal women gave 3.4 times risk(OR = 3.4; 95%CI: 1.204-11.787; P = 0.011)for symptomatic lumbar OA than the low ratio of IL-6/IL-10 level(cut-off point 0.364).CONCLUSION High ratio of IL-6/IL-10 plasma level was the highest risk factor for causing symptomatic lumbar OA in postmenopausal women with estrogen deficiency. | I Ketut Suyasa I Ketut Siki Kawiyana I Made Bakta I Gde Raka Widiana | 2017 | World Journal of Orthopedics2017，8，2： | 7 |
| 4 | A study on centrifugal casting of high speed steel roll显示文摘High speed steel (HSS) rolls can replace traditional rolls such as alloyed cast iron rolls and powder metallurgical (PM) hard alloy rolls. The main reasons for the replacement are that the wear resistance of low-cost alloyed cast iron rolls is poor and the cost of high-quality PM hard alloy rolls is very high. By means of centrifugal casting, HSS rolls having excellent wear resistance have been manufactured. The hardness of the HSS roll is 6.5~67 HRC, the range of variation is smaller than 2 HRC and its impact toughness is 15 J/cm2. The wear rate of HSS rolls used in the pre-finishing stands of high-speed hot wire-rod rolling mill reaches 2.5×10-4mm per ton steel. Furthermore, the manufacturing cost of HSS rolls is significantly lower than that of PM hard alloy rolls; it is only 30 percent of that of PM hard alloy rolls. | ~*H. G. Fu~1, X.H. Zhang~2(1. Department of Mechanical engineering, Tsinghua University, Beijing 100084, P. R. China 2. Roll Made LTD. of Taiyuan Iron and Steel(Group)Company, Taiyuan 030003, Shanxi, P. R. China) | 2004 | China Foundry2004，1，S1： | 6 |
| 5 | Serum testosterone and prostate-specific antigen levels are major risk factors for prostatic volume increase among benign prostatic hyperplasia patients显示文摘Objective:Benign prostatic hyperplasia(BPH)is one of the most common diseases found among elderly men.Even though multiple risk factors of BPH have been identified in the past,the risk factors which have a direct impact on prostate volume have not been identified.In this study,we aim to determine the most significant contributing risk factors to prostate volume enlargement by analyzing possible associated risk factors previously studied.Methods:This is a quantitative study with an analytical observational design,performed using a retrospective cohort approach.Total sampling was performed on 83 patients who underwent transurethral resection of the prostate(TURP)in Sanglah General Hospital from January to February 2019.Bivariate analysis is performed to examine each variable's association with prostate volume followed by a multivariate analysis.All variables were reassessed with path analysis to measure the direct effects,indirect effects,and total effects on prostate volume.Results:Bivariate analysis shows that serum testosterone(R=0.208;p=0.059)and prostate-specific antigen(PSA)level(R=0.626;p=0.001)have a significant association with prostate volume.Multivariate analysis shows that serum PSA(B=1.4;p=0.001;95%confidence interval[95%CI]=1.039-1.770)and testosterone(B=0.024;p=0.005;95%CI=0.008-0.041)levels are significant among all the analyzed risk factors.There is a significant and strong effect of PSA to prostate volume(c=0.636;p=0.001)whereas testosterone has a significant albeit weak effect to prostate volume(c=0.246;p=0.009)based on the total effect of the path analysis.Conclusion:Serum testosterone and PSA levels are significantly associated with prostatic volume increase among BPH patients. | Gede Wirya Kusuma Duarsa Yudit Anastasia Sari Anak Agung Gde Oka Kadek Budi Santosa I Wayan Yudiana Pande Made Wisnu Tirtayasa Ida Bagus Putra Pramana Yudhistira Pradnyan Kloping | 2021 | Asian Journal of Urology2021，8，3： | 3 |
| 6 | Establishment and characterization of cell sublines with high and low metastatic potential derived from human osteosarcoma显示文摘The most frequent cause of death of patients with osteosarcoma is the metastasis of tumour cells. In spite of successful control of the primary tumour, the mortality of the patients due to metastatic spread is more than 30% within 5 years. 1 Recent studies about osteosarcoma metastatic mechanism are based on osteosarcoma matrilineal cell lines. 2 For further studies of metastatic mechanism of osteosarcoma the establishment of a better metastatic experimental model of osteosarcoma is needed. We isolated and established two cell sublines, with high and low metastatic potentials, respectively, derived from human osteosarcoma MG-63 cell line by cloning in vitro and transplantation in vivo , then analysed and identified their biological characteristics. | SHIXiao-bing CHENAn-ming CAIXian-hua GUOFen-jing LIAOGuo-ning MADing | 2005 | Chinese Medical Journal2005，，8： | 2 |
| 7 | miR-133 and miR-30 Regulate Connective Tissue Growth Factor: Implications for a Role of MicroRNAs in Myocardial Matrix Remodeling显示文摘 | Rudy F. Duisters Anke J. Tijsen Blanche Schroen Joost J. Leenders Viola Lentink Ingeborg van der Made Veronica Herias Rick E. van Leeuwen Mark W. Schellings Paul Barenbrug Jos G. Maessen Stephane Heymans Yigal M. Pinto Esther E. Creemers | 2009 | Circulation Research2009，，： | 2 |
| 8 | Endothelial Cell-Specific NF-κB Inhibition Protects Mice from Atherosclerosis显示文摘 | Ralph Gareus Elena Kotsaki Sofia Xanthoulea Ingeborg van der Made Marion J.J. Gijbels Rozina Kardakaris Apostolos Polykratis George Kollias Menno P.J. de Winther Manolis Pasparakis | 2008 | Cell Metabolism2008，，5： | 2 |
| 9 | Basic previous term hydrol- ysis next term 2- fluoro-1,3,4,6-tetra-O-acetyl-image-glu- cose in the preparation of 2-显示文摘 | Fuchtner F Steinbach J Mading P | 1996 | Appl Radiat Isot1996，47，： | 1 |
| 10 | Homogeneous catalysts based on silane dendrimers functionalized with arylnickel(Ⅱ) complex显示文摘 | Knapen J W J van der Made A W de Wilde J C | | 0，，： | 1 |
| 11 | Optimization of a predictive controller for the closed loop adaptive optics显示文摘 | C Dessenne P Y Madee G Rousset | 1998 | Appl Opt1998，37，21： | 1 |
| 12 | Observations on the different methods of processing on the bacterial contaminants of bovine and ovine tripe显示文摘 | van der MADE H N van STADEN J J | 1978 | Onderstepoort Journal of Veterinary Research1978，45，： | 1 |
| 13 | Perspectives of School-to-Career Programs显示文摘 | Bonnie Made Oughty Jenkins | 2005 | Techniques: Connecting Education and careers2005，，7： | 1 |
| 14 | Changes in reactive surface area during limestone dissolution: An experimental and modelling study显示文摘 | Noiriel C Luquot L Made B Raimbault L Gouze P van der Lee J | 2009 | Chem Geol2009，265，12： | 1 |
| 15 | A real-time PCR for detection of pathogenic Yersinia enterocolitica in food combined with an universal internal amplification control system显示文摘 | MADED REITING R STRAUCH E | 2008 | Journal of Con-sumer Protection and Food Safety2008，3，2： | 1 |
| 16 | Dealing with Project Complexity by Matrix - based Propagation Modelling for Project Risk Analysis 显示文摘 | Chao Fang Franck Made | 2013 | Journal of Engineering Design2013，24，4： | 1 |
| 17 | Ventilation strategy using low tidal volumes,recruitment maneuvers,and high positive end-expiratory pressure for acute lung injury and acute respiratory distress syndrome:a randomized controlled trial显示文摘 | MADE M O COOK D J GUYATT G H | 2008 | JAMA2008，299，6： | 1 |
| 18 | Accumu- lating progenitor cells in the luminal epithelial cell layer are candidate tumor initiating cells in a Pten knockout mouse pros- tate cancer model显示文摘 | KORSTEN H ZIEL-van der MADE A MA X | 2009 | PLoS One2009，4，5： | 1 |
| 19 | Broadened ligand responsiveness of androgen receptor mutants obtained by random amino acid substitution of 1-1874 andmutation hot spot T877 in prostate cancer 显示文摘 | Steketee K Timmerman L Ziel-van der Made AC | 2002 | Int J Cancer2002，100，： | 1 |
| 20 | Detection of genetically modified rice:A construct-specific real-time PCR method based on DNA sequences from transgenic Bt rice显示文摘 | Dietrich Made Degner C Grohmann L | 2006 | Eur Food Res Technol2006，224，： | 1 |
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