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| 1 | Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification显示文摘AIM:To study whether selected bacterial 16S ribosomal RNA(rRNA)gene phylotypes are capable of disting- uishing irritable bowel syndrome(IBS). METHODS:The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant (IBS-D),eight constipation-predominant(IBS-C)and four mixed symptom-subtype(IBS-M)IBS patients,and fifteen control subjects,were analysed at three time-points with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis(PCA)with linear mixed-effects models applied to the principal component scores. RESULTS:Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component(PC1),explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups(IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05).Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy- lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects(-4.08±0.90 vs -3.33±1.16,P=0.04)and IBS-D and IBS-M subjects (-4.08±0.90 vs-3.08±1.38,P=0.05).Furthermore,a phylotype with 94%similarity to R.torques was more prevalent in IBS-D patients'intestinal micro- biota than in that of control subjects(-2.43±1.49 vs -4.02±1.63,P=0.01).A phylotype with 93%simi- larity to R.torques was associated with control sam- ples when compared with IBS-M(-2.41±0.53 vs -2.92±0.56,P=0.00).Additionally,a R.bromii-like phylotype was associated with IBS-C patients in com- parison to control subjects(-1.61±1.83 vs-3.69± 2.42,P=0.01).All of the above mentioned phylotype specific alterations were independent of the effect of time. CONCLUSION:Significant phylotype level alterationsin the intestinal microbiotas of IBS patients were observed,further emphasizing the possible contribution of the gastrointestinal microbiota in IBS. | Anna Lyra Teemu Rinttil Janne Nikkil Lotta Krogius-Kurikka Kajsa Kajander Erja Malinen Jaana Mtt Laura Mkel Airi Palva | 2009 | World Journal of Gastroenterology2009，15，47： | 26 |
| 2 | High-fat-induced intestinal permeability dysfunction associated with altered fecal bile acids显示文摘AIM:To investigate whether high-fat-feeding is associated with increased intestinal permeability via alterations in bile acid metabolism.METHODS:Male C57Bl/6J mice were fed on a high-fat (n=26) or low-fat diet (n=24) for 15 wk.Intestinal permeability was measured from duodenum,jejunum,ileum and colon in an Ussing chamber system using 4 kDa FITC-labeled dextran as an indicator.Fecal bile acids were analyzed with gas chromatography.Segments of jejunum and colon were analyzed for the expression of farnesoid X receptor (FXR) and tumor necrosis factor (TNF).RESULTS:Intestinal permeability was significantly increased by high-fat feeding in jejunum (median 0.334 for control vs 0.393 for high-fat,P=0.03) and colon (0.335 for control vs 0.433 for high-fat,P=0.01),but not in duodenum or ileum.The concentration of nearly all identified bile acids was significantly increased by high-fat feeding (P<0.001).The proportion of ursodeoxycholic acid (UDCA) in all bile acids was decreased (1.4%±0.1% in high-fat vs 2.8%±0.3% in controls,P<0.01) and correlated inversely with intestinal permeability (r=-0.72,P=0.01).High-fat feeding also increased jejunal FXR expression,as well as TNF expression along the intestine,especially in the colon.CONCLUSION:High-fat-feeding increased intestinal permeability,perhaps by a mechanism related to bile acid metabolism,namely a decreased proportion of fecal UDCA and increased FXR expression. | Lotta K Stenman Reetta Holma Riitta Korpela | 2012 | World Journal of Gastroenterology2012，18，9： | 19 |
| 3 | Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome显示文摘AIM:To investigate the correlations between selfreported symptoms of irritable bowel syndrome(IBS) and the gastrointestinal(GI) microbiota composition.METHODS:Fecal samples were collected from a total of 44 subjects diagnosed with IBS.Their symptoms were monitored with a validated inflammatory bowel disease questionnaire adjusted for IBS patients.Thirteen quantitative real-time polymerase chain reaction assays were applied to evaluate the GI microbiota composition.Eubacteria and GI bacterial genera(Bifidobacterium,Lactobacillus and Veillonella),groups(Clostridium coccoides/Eubacterium rectale,Desulfovibrio desulfuricans) and distinct bacterial phylotypes [closest 16S rDNA sequence resemblance to species Bifidobacterium catenulatum,Clostridium cocleatum,Collinsella aerofaciens(C.aerofaciens),Coprococcus eutactus(C.eutactus),Ruminococcus torques and Streptococcus bovis ] with a suspected association with IBS were quantified.Correlations between quantities or presence/absence data of selected bacterial groups or phylotypes and various IBSrelated symptoms were investigated.RESULTS:Associations were observed between subjects' self-reported symptoms and the presence or quantities of certain GI bacteria.A Ruminococcus torques(R.torques)-like(94% similarity in 16S rRNA gene sequence) phylotype was associated with severity of bowel symptoms.Furthermore,among IBS subjects with R.torques 94% detected,the amounts of C.cocleatum 88%,C.aerofaciens-like and C.eutactus 97% phylotypes were significantly reduced.Interesting observations were also made concerning the effect of a subject's weight on GI microbiota with regard to C.aerofaciens like phylotype,Bifidobacterium spp.and Lactobacillus spp.CONCLUSION:Bacteria seemingly affecting the symptom scores are unlikely to be the underlying cause or cure of IBS,but they may serve as biomarkers of the condition. | Erja Malinen Lotta Krogius-Kurikka Janne Nikkil Airi Palva Anna Lyra Anne Jskelinen Teemu Rinttil Terttu Vilpponen-Salmela Atte Johannes von Wright | 2010 | World Journal of Gastroenterology2010，16，36： | 13 |
| 4 | DGGE法与常规培养法对稻田蓝细菌多态性分析结果比较显示文摘研究运用蓝细菌和硅藻16SrDNA特异引物,将晚季水稻生长后期稻田土壤中提取的总DNA进行PCR扩增后,以DGGE技术对PCR产物进行分析结果表明,14条DGGE带经克隆测序,经NCBI基因库比对得晚季水稻生长后期存在10种蓝细菌,包括4种Leptolyngbya、1种Chamaesiphon、1种Nostoc、1种Oscillatoria、2种Syne-chococcus和1种Chroococcidiopsis。同层不同位置土壤中蓝细菌种群亦有所不同,但每个取样点都有一些特有的蓝细菌种类。用常规方法对同一稻田土壤样品进行分离培养,根据蓝细菌鉴定图谱观察到类似Lyngbya、Oscillatori-a、Chroococcidiopsis及Nostoc的蓝细菌,但显微镜下无法准确分类。比较结果表明采用DGGE法比常规培养法能更准确进行蓝细菌多态性鉴定。 | 宋铁英 林智敏 郑伟文 LOTTA Martensson ULLA Rusmussen | 2005 | 中国生态农业学报2005，13，3： | 4 |
| 5 | 腹腔镜下或胃镜下胃食管反流手术的疗效观察显示文摘目的探讨腹腔镜下或胃镜下胃食管反流(gastroesophageal reflux disease,GERD)手术的疗效、手术方法及适应证。方法总结2005年8月~2006年1月施行腹腔镜下Nissen手术52例和胃镜下胃壁折叠术28例的一般资料、手术结果,以及术后1、3个月的随访资料。结果两组的症状指数及反流时间均明显下降。腹腔镜组灼心感指数和反流指数分别从72.2±7.3和1.80±0.45下降至14.5±4.8和0.26±0.15(P均〈0.05);胃镜组该两指数分别从70.6±9.4和1.80±0.32下降至28.2±7.6和0.30±0.12(P均〈0.05)。腹腔镜组食管下段反流时间百分比从11.2%±6.2%下降至2.5%±0.4%(P〈0.05);胃镜组从10.2%±5.7%下降至7.6%±1.2%(P〈0.05)。腹腔镜手术中1例中转开腹手术,近期并发症3例,远期并发症2例,术后完全缓解率93.9%(46/49)。胃镜手术近期并发症3例,无远期并发症,术后完全缓解率58.3%(14/24)。结论腹腔镜下Nissen手术是治疗严重GERD的理想术式,胃镜下胃折叠术则适用于治疗病情轻的GERD以减少其对药物的依赖,但疗效尚待进一步提高。 | 李旭 涂远荣 Lotta Orre | 2006 | 中国微创外科杂志2006，6，10： | 4 |
| 6 | 基于试验室及道路模拟试验钢渣沥青混合料的性能评价显示文摘钢铁渣的固有特性使其成为沥青混合料用的天然集料或人工破碎集料的理想替代品,可以确定的是,除了环境效益,使用钢渣集料还可以生产出性能优于天然集料的沥青混合料。该文研究的目的是评价10种不同钢渣材料的性能,通过对钢渣集料和钢渣沥青混合料的室内试验,确定不同钢渣材料的磨耗性和永久变形性。此外,还对其中一种钢渣制成的两种不同类型的沥青路面混合料进行了道路模拟试验,以评估其磨耗性,并测量镶钉轮胎产生的颗粒排放(PM10)。试验结果表明:不同的矿渣材料具有超过或匹配优质岩石的集料特性,与参考混合料相比,钢渣沥青混合料具有更好的短期和长期车辙性能;钢渣沥青混合料产生的PM10质量分布与传统沥青混合料相当。 | 王超(编译) 张彩利(编译) 赵辉(编译) Abubeker W.Ahmed Leif Viman Lotta Lind | 2022 | 中外公路2022，42，2： | 2 |
| 7 | Kinetics of human soluble and membrane-bound catechol O-methyltransferase:a revised mechanism and description of the thermolabile variant of the enzyme显示文摘 | Lotta T Vidgren J Tilgmann C | 1995 | Biochemistry1995，34，： | 1 |
| 8 | Kinetics of human soluble and membrane-bound catechol O-meth yltransferase:a revised mechanism and description of the thermolabile variant of the enzyme显示文摘 | Lotta T Vidqren J Tilqmann C | | 0，，13： | 1 |
| 9 | Developments in Cancer Immunotherapy显示文摘 | Maries van den B Lotta von B Alexander K | 2010 | Dig Dis2010，28，： | 1 |
| 10 | Cancer metastasis and angiogenesis:an inbalance of positive and negative regulation 显示文摘 | Lotta LA steeg Ps settler- stevenson WG | 1991 | Cell1991，64，： | 1 |
| 11 | Pathogenesis and treatment of ac- quired idiopathic thrombotic thrombocytopenic purpura 显示文摘 | Peyvandi F Palla R Lotta LA | 2010 | Hae- matologica2010，95，9： | 1 |
| 12 | Inhibition of lymphangiogenesis with resulting lymphedema in transgenic mice expressing soluble VEGF receptor-3 显示文摘 | Taija M Lotta J Tanja V | 2001 | Nature Medicine2001，7，2： | 1 |
| 13 | Emerging design strategies in sustainable production and consumption of textiles and clothing显示文摘 | Kirsi Niinim(a)ki Lotta Hassi | | 0，，16： | 1 |
| 14 | Microvascular changes in the lower extremities of paraplegics with heterotopic ossification 显示文摘 | Lotta S Scelsi L Scelsi R | 2001 | Spinal Cord2001，39，11： | 1 |
| 15 | Guidelines for the use and interpretation of assays for monitoring autophagy显示文摘 | Daniel J. Klionsky Fabio C. Abdalla Hagai Abeliovich Robert T. Abraham Abraham Acevedo-Arozena Khosrow Adeli Lotta Agholme Maria Agnello Patrizia Agostinis Julio A. Aguirre-Ghiso Hyung Jun Ahn Ouardia Ait-Mohamed Slimane Ait-Si-Ali Takahiko Akematsu Shizu | 2012 | Autophagy2012，，4： | 1 |
| 16 | Tyrosine kinase inhibitor usage, treatment outcome, and prognostic scores in CML: report from the population-based Swedish CML registry显示文摘 | Martin H?glund Fredrik Sandin Karin Hellstr?m Mats Bj?reman Magnus Bj?rkholm Mats Brune Arta Dreimane Marja Ekblom S?ren Lehmann Per Ljungman Claes Malm Berit Markev?rn Kristina Myhr-Eriksson Lotta Ohm Ulla Olsson-Str?mberg Anders Sj?lander Hans Wadenvik | 2013 | Blood2013，，： | 1 |
| 17 | ADAMTS-13 assays in thrombotic thrombocytopenic purpura 显示文摘 | Peyvandi F Palla R Lotta LA et aI | 2010 | J Thromb Haemost2010，8，4： | 1 |
| 18 | ADAMTS13 mutations and polymorphisms in congenital thrombotic thrombocytopenic purpura显示文摘 | Lotta L A Garagiola I Palla R | 2010 | HumMutat2010，31，1： | 1 |
| 19 | Vascular endothelial growth factor-C-mediated lymphangiogenesis promotes tumour metastasis 显示文摘 | Stefano J Mandriota Lotta Jussila | 2001 | EMBO J2001，20，4： | 1 |
| 20 | Signalling viavascular endothelial growth factor receptor-3 is sufficient for lymphangiogenesis in transgenic mice 显示文摘 | Tanja Veikkola Lotta Jussila Taija Makinen | 2001 | EMBO J2001，20，6： | 1 |
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