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| 1 | Engineering Escherichia coli to improve tryptophan production via genetic manipulation of precursor and cofactor pathways显示文摘Optimizing the supply of biosynthetic precursors and cofactors is usually an effective metabolic strategy to improve the production of target compounds.Here,the combination of optimizing precursor synthesis and balancing cofactor metabolism was adopted to improve tryptophan production in Escherichia coli.First,glutamine synthesis was improved by expressing heterologous glutamine synthetase from Bacillus subtilis and Bacillus megaterium in the engineered Escherichia coli strain KW001,resulting in the best candidate strain TS-1.Then icd and gdhA were overexpressed in TS-1,which led to the accumulation of 1.060 g/L tryptophan.Subsequently,one more copy of prs was introduced on the chromosome to increase the flux of 5-phospho-α-D-ribose 1-diphosphate followed by the expression of mutated serA and thrA to increase the precursor supply of serine,resulting in the accumulation of 1.380 g/L tryptophan.Finally,to maintain cofactor balance,sthA and pntAB,encoding transhydrogenase,were overexpressed.With sufficient amounts of precursors and balanced cofactors,the engineered strain could produce 1.710 g/L tryptophan after 48 h of shake-flask fermentation,which was 2.76-times higher than the titer of the parent strain.Taken together,our results demonstrate that the combination of optimizing precursor supply and regulating cofactor metabolism is an effective approach for high-level production of tryptophan.Similar strategies could be applied to the production of other amino acids or related derivatives. | Zhu Li Dongqin Ding Huiying Wang Linxia Liu Huan Fang Tao Chen Dawei Zhang | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 5 |
| 2 | Study on growing thick AlGaN layer on c-plane sapphire substrate and free-standing GaN substrate显示文摘In this study,the thick AlGaN epilayers have been grown on the c-plane sapphire substrate and the free-standing GaN substrate using low-temperature AlN nucleation layers by low-pressure metal-organic chemical vapor deposition(LPMOCVD).High resolution X-ray diffraction(HRXRD),atom force microscopy(AFM),scanning electron microscopy(SEM),photoluminescence(PL) and Raman scattering measurements have been employed to study the crystal quality,threading dislocation density,surface morphology,optical properties and phonon properties of thick AlGaN epifilms.The results indicate that AlGaN epifilms crystal quality can be improved greatly when grown on the free-standing GaN substrate.We calculated the threading dislocation density and found that thick AlGaN epifilm grown on the free-standing GaN substrate is much lower in total threading dislocation density than that grown on the sapphire substrate,although the surface morphology is rougher than that of sapphire substrate. | WANG DangHui ZHOU Hao ZHANG JinCheng XU ShengRui ZHANG LinXia MENG FanNa AI Shan HAO Yue | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，12： | 3 |
| 3 | Heavy ion micro-beam study of single-event transient(SET) in Si Ge heterjunction bipolar transistor显示文摘Silicon-germainum heterojunction bipolar transistor(Si Ge HBT)has been demonstrated to be suitable in extreme environment because of its superior temperature characteristics which can operate from 43 K to 400 K[1].In addition,outstanding hardness to both total ionizing dose(TID)radiation and displacement damage make Si Ge HBT technology particularly attractive for | Jinxin ZHANG Hongxia GUO Fengqi ZHANG Chaohui HE Pei LI Yunyi YAN Hui WANG Linxia ZHANG | 2017 | Science China(Information Sciences)2017，60，12： | 2 |
| 4 | Preparation, prop- erties and applications of polypyrtoles 显示文摘 | Wang Linxia Li Xingui Yang Yuliang | 2001 | Reactive and Functional Polymers2001，47，2： | 1 |
| 5 | Preparation of lead carbonate from spent lead paste via chemical conversion显示文摘 | Xinfeng Zhu Jiakuan Yang Linxia Gao Jianwen Liu Danni Yang Xiaojuan Sun Wei Zhang Qin Wang Lei Li Dongsheng He R. Vasant Kumar | 2013 | Hydrometallurgy2013，，： | 1 |
| 6 | Synthesis and characterization of pyrrole and anisidine copolymers显示文摘 | LI Xingui HUANG Meirong WANG Linxia | 2001 | Polymer2001，42，14： | 1 |
| 7 | Synthesis and characterization of pyrrole and m-toluidine copolymers 显示文摘 | LI Xingui HUANG Meirong WANG Linxia | 2001 | Synthetic Metals2001，123，3： | 1 |
| 8 | A cyclin protein governs the infectious and sexual life cycles of Cryptococcus neoformans显示文摘Cell cycle is a fundamental process underlying growth and development in evolutionarily diverse organisms, including fungi. In human fungal pathogens, cell cycle control generally determines their life cycles, either in the environment or during infections.Thus, cell cycle components can potentially serve as important targets for the development of antifungal strategy against fungal infections. Here, in Cryptococcus neoformans, the most common cause of fatal fungal meningitis, we show that a previously uncharacterized B-type cyclin named Cbc1 is essential for both its infectious and sexual cycles. We reveal that Cbc1 coordinates various sexual differentiation and molecular processes, including meiosis. Especially, the absence of Cbc1 abolishes formation of sexual spores in C. neoformans, which are presumed infectious particles. Cbc1 is also required for the major Cryptococcus pathogenic attributes. Virulence assessment using the murine model of cryptococcosis revealed that the cbc1 mutant is avirulent.Together, our results provide an important insight into how C. neoformans employs shared cell cycle regulation to coordinate its infectious and sexual cycles, which are considered crucial for virulence evolution and the production of infectious spores. | Pengjie Hu Linxia Liu Weixin Ke Xiuyun Tian Linqi Wang | 2021 | Science China(Life Sciences)2021，64，8： | 1 |
| 9 | Leaching of spent lead acid battery paste components by sodium citrate and acetic acid显示文摘 | Xinfeng Zhu Xiong He Jiakuan Yang Linxia Gao Jianwen Liu Danni Yang Xiaojuan Sun Wei Zhang Qin Wang R Vasant Kumar | 2013 | Journal of Hazardous Materials2013，，： | 1 |
| 10 | Leaching of spent lead acid battery paste components by sodium citrate and acetic acid显示文摘 | Xinfeng Zhu Xiong He Jiakuan Yang Linxia Gao Jianwen Liu Danni Yang Xiaojuan Sun Wei Zhang Qin Wang R Vasant Kumar | 2013 | Journal of Hazardous Materials2013，，： | 1 |
| 11 | Polyvinylpyrrolidone as an Efficient Stabilizer for Silver Nanoparticles显示文摘Aggregation of nanoparticles is a serious problem in their applications.In this article,stability of biosynthesized silver nanoparticles(AgNPs)was initially studied with different concentrations of sodium chloride(NaCl).Surfactant screening showed that polyvinylpyrrolidone(PVP)was an ideal stabilizer,which not only was effective at a low concentration(0.1%,V/V),but also could protect silver colloids from aggregation for a long period at 1 mol•L^(−1) NaCl,and 6 h even at 3 mol•L^(−1) NaCl.However,some other surfactants,such as sodium dodecyl(SDS),Triton X-100,Tween-20,and Tween-80,could not prevent AgNPs from aggregation even at a high concentration up to 0.5%(V/V).This AgNPs stabilizer is simple and convenient for a variety of applications compared with others. | Weidong Huang Linxia Zhang Qiuping Yang Zhimin Wang | 2014 | Chinese Journal of Chemistry2014，32，9： | 0 |
| 12 | HfAlO-based ferroelectric memristors for artificial synaptic plasticity显示文摘Memristors have received much attention for their ability to achieve multi-level storage and synaptic learning.However,the main factor that hinders the application of memristors to simulate neural synapses is the instability of the formation and breakage of conductive filaments inside traditional memristors,which makes it difficult to simulate the function of biological synapses in practice.However,the resistance change of ferroelectric memristors relies on the polarization inversion of the ferroelectric thin film,thus avoiding the above problem.In this study,a Pd/HfAlO/LSMO/STO/Si ferroelectric memristor is proposed,which can achieve resistive switching properties through the combined action of ferroelectricity and oxygen vacancies.The I−V curves show that the device has good stability and uniformity.In addition,the effect of pulse sequence modulation on the conductance was investigated,and the biological synaptic function and learning behavior were simulated successfully.The results of the above studies provide a basis for the development of ferroelectric memristors with neurosynaptic-like behaviors. | Jie Yang Zixuan Jian Zhongrong Wang Jianhui Zhao Zhenyu Zhou Yong Sun Mengmeng Hao Linxia Wang Pan Liu Jingjuan Wang Yifei Pei Zhen Zhao Wei Wang Xiaobing Yan | 2023 | Frontiers of physics2023，18，6： | 0 |
| 13 | Local sustained release of PD-1 monoclonal antibody and lenvatinib by thermo-sensitive hydrogel for improving tumor immunotherapy显示文摘In clinic,the combination of intravenous pembrolizumab(PD-1 monoclonal antibody)with oral Lenvatinib(LEN)exhibited an enhanced synergistic benefit for cancer therapy.However,the clinical outcomes were always limited by the problems of inconsistent pharmacokinetic profiles of two drugs,lower drug accumulation in tumor and obvious side effects during the combination therapy.Here,in situ-forming thermosensitive hydrogels based on PLGA-PEG-PLGA triblock copolymers were prepared for local administration of anti-PD1 and LEN(P&L@Gel)to improve therapeutic efficacy and safety.After peritumoral or surgical resection site injection,the significant increased concentrations of both drugs in tumor were observed with the local sustained release of P&L@Gel.In comparison with the group of intraperitoneal anti-PD1 plus oral LEN(P-ip&L-po),significantly higher tumor inhibition efficiency on CT26 tumor models could be obtained in P&L@Gel group,even at the dose of one-eighth of the former,same tumorinhibition effects could be achieved.The enhanced antitumor efficacy of P&L@Gel group was probably associated with the 2.2 folds of increased level of CD8+T cells and the polarization of tumor associated macrophage from M2 to M1 along with the increased drug accumulation.Moreover,compared with the obvious side effects of P-ip&L-po group,no significant changes of PLT,ALT and UA in blood,as well as IL-1αand IL-1βin mice paws were observed between P&L@Gel group and untreated group.These results suggested that local administration of anti-PD1 and LEN with thermosensitive hydrogel could offer a potential strategy for tumors or tumor postoperative adjuvant treatment. | Lin Zhai Yujie Shi Yi Yan An Lu Xiaoyu Liu Lei Lei Yi Sun Linxia Jiang Xiangyu Wang Honggang Qian Jiancheng Wang | 2023 | Chinese Chemical Letters2023，34，8： | 0 |
| 14 | Amplitude-frequency-aware deep fusion network for optimal contact selection on STN-DBS electrodes显示文摘Parkinson’s disease(PD)is treated effectively by deep brain stimulation(DBS)of the subthalamic nucleus(STN),using an electrode inserted into the head of a PD patient.The electrode has multiple electrical contacts along its length,so the best may be chosen for selectively stimulating the STN.Neurosurgeons usually determine the optimal stimulated contact via the clinical experience of the neurosurgeon and the motor improvement of PD patients.This is a time-consuming and labor-intensive trial-and-error process.The selection of optimal stimulated contact highly depends on the locations of sweet spots,which are manually identified by the characteristic features of microelectrode recordings(MERs).This paper presents an amplitude-frequency-aware deep fusion network for optimal contact selection on STN-DBS electrodes.The method first obtains the amplitude-frequency fusion features by combining the MERs time sequence features and the amplitude sequence features,and then uses the convolutional neural network(CNN)with convolutional block attention module(CBAM)to identify both the border of the STN and the sweet spots to implant the electrode.The optimal stimulated contact can be selected according to the distribution of the sweet spots.Experimental results indicate that,for successful surgeries,neurosurgeons and the proposed AI solution selected the same optimal contacts.Furthermore,the proposed method outperforms the state-of-the-art methods for STN and sweet spot identification.The proposed method shows great potential for optimal contact selection to improve the efficiency of STN-DBS surgery and reduce the dependence on clinicians’experience. | Linxia XIAO Caizi LI Yanjiang WANG Weixin SI Hai LIN Doudou ZHANG Xiaodong CAI Pheng-Ann HENG | 2022 | Science China(Information Sciences)2022，65，4： | 0 |
| 15 | Single-cell RNA-seq of Lotus japonicus provide insights into identification and function of root cell types of legume显示文摘The roots of legume plant play a crucial role in nitrogen fixation. However, the transcriptomes of different cell types of legume root and their functions remain largely unknown. Here, we performed single-cell RNA sequencing and profiled more than 22,000 single cells from root tips of Lotus japonicus, a model species of legume.We identified seven clusters corresponding to seven major cell types, which were validated by in situ hybridization. Further analysis revealed regulatory programs including phytohormone and nodulation associated with specific cell types, and revealed conserved and diverged features for the cell types. Our results represent the first single-cell resolution transcriptome for legume root tips and a valuable resource for studying the developmental and physiological functions of various cell types in legumes. | Zhanmin Sun Sanjie Jiang Dan Wang Linxia Li Boxin Liu Qifan Ran Lizhen Hu Junbo Xiong Yixiong Tang Xiaofeng Gu Yanmin Wu Zhe Liang | 2023 | Journal of Integrative Plant Biology2023，65，5： | 0 |
| 16 | Poverty Elimination: An Innate Component of Contemporary Basic Concept of Human Rights显示文摘In 1969, the UN delivered its Declaration on Social Progress and Development, clearly call- ing for 'the elimination of poverty; the assurance of a | WANG LINXIA | 2011 | The Journal of Human Rights2011，10，4： | 0 |
| 17 | Microstrip Patch Antenna with an Inverted T-Type Notch in the Partial Ground for Breast Cancer Detections显示文摘This study designs a microstrip patch antenna with an inverted T-type notch in the partial ground to detect tumorcells inside the human breast.The size of the current antenna is small enough(18mm×21mm×1.6mm)todistribute around the breast phantom.The operating frequency has been observed from6–14GHzwith a minimumreturn loss of−61.18 dB and themaximumgain of current proposed antenna is 5.8 dBiwhich is flexiblewith respectto the size of antenna.After the distribution of eight antennas around the breast phantom,the return loss curveswere observed in the presence and absence of tumor cells inside the breast phantom,and these observations showa sharp difference between the presence and absence of tumor cells.The simulated results show that this proposedantenna is suitable for early detection of cancerous cells inside the breast. | Nure Alam Chowdhury Lulu Wang Md Shazzadul Islam Linxia Gu Mehmet Kaya | 2024 | Computer Modeling in Engineering & Sciences2024，138，2： | 0 |
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