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| 1 | Carboniferous Post-collisional Rift Volcanism of the Tianshan Mountains, Northwestern China显示文摘The Tianshan Carboniferous post-collisional rift volcanic rocks occur in northwestern China as a large igneous province. Based on petrogeochemical data, the Tianshan Carboniferous post-collisional rift basic lavas can be classified into two major magma types: (1) the low-Ti/Y type situated in the eastern-central Tianshan area, which exhibits low Ti/Y (<500), Ce/Yb (<15) and SiO2 (43-55%), and relatively high Fe2O3T (6.4-11.5%); (2) the high-Ti/Y type situated in the western Tianshan area, which has high Ti/Y (>500), Ce/Yb (>11) and SiO2 (49-55%), and relatively low Fe2O3T (5.8-7.8%). Elemental data suggest that chemical variations of the low-Ti/Y and high-Ti/Y lavas cannot be explained by fractional crystallization from a common parental magma. The Tianshan Carboniferous basic lavas originated most likely from an OIB-like asthenospheric mantle source (87Sr/86Sr(t) ≈ 0.703-0.705, εNd(t) ≈ +4 to +7). The crustal contamination and continental lithospheric mantle have also contributed significantly to the formation of the basic lavas of the Tianshan Carboniferous post-collisional rift. The silicic lavas were probably generated by partial melting of the crust. The data of this study show that spatial petrogeochemical variations exist in the Carboniferous post-collisional rift volcanics province in the Tianshan region. Occurrence of the thickest volcanics dominated by tholeiitic lavas may imply that the center of the mantle-melting anomaly (mantle plume) was in the eastern Tianshan area at that time. The basic volcanic magmas in the eastern Tianshan area were generated by a relatively high degree of partial melting of the mantle source around the spinel-garnet transition zone, whereas the alkaline basaltic lavas are of the dominant magma type in the western Tianshan area, which were generated by a low degree of partial melting of the mantle source within the stable garnet region, thus the basic lavas of the western Tianshan area might have resulted from relatively thick lithosphere and low geothermal gradient. | XIA Linqi, XU Xueyi, XIA Zuchun, LI Xiangmin, MA Zhongping and WANG Lishe Xi’an Institute of Geology and Mineral Resources, China Geological Survey, Xi’an, Shaanxi 710054 E-mail: geologyx@pub.xaonline.com. Liu Shuchun and Liu Xinzhu | 2003 | Acta Geologica Sinica(English Edition)2003，77，3： | 49 |
| 2 | Structure of MERS-CoV spike receptor-binding domain complexed with human receptor DPP4显示文摘最近的尖铁 glycoprotein (S) 鉴别中东呼吸症候群 coronavirus (MERS-CoV ) 指向细胞的受体, dipeptidyl peptidase 4 (DPP4 ) 。顺序比较和当模特儿的分析在病毒的尖铁上揭示了一个通常认为的受体绑定领域(RBD ) ,它调停这个相互作用。我们报导 3.0 Å MERS-CoV RBD 的决定水晶结构跳了到人的 DPP4 的细胞外的领域。我们的结果证明 MERS-CoV RBD 由一个核心和受体绑定子域组成。受体绑定子域与 DPP4 β 交往;推进器然而并非它的内在的 hydrolase 领域。MERS-CoV RBD 和相关 SARS-CoV RBD 分享他们的核心子域的结构的类似的高度,但是在受体绑定子域是尤其是分叉的。Mutagenesis 研究在为到 DPP4 和入口的病毒的绑定是批评的进目标房间的受体绑定子域识别了几关键残余。在在 MERS-CoV RBD 和 DPP4 之间的接口的原子细节提供病毒和受体相互作用的结构的理解,它能对 MERS-CoV 感染指导治疗学和疫苗的开发。 | Nianshuang Wang Xuanling Shi Liwei Jiang Senyan Zhang Dongli Wang Pei Tong Dongxing Guo Lili Fu Ye Cui Xi Liu Kelly C Arledge Ying-Hua Chen Linqi Zhang Xinquan Wang | 2013 | Cell Research2013，23，8： | 26 |
| 3 | Relationships between Basic and Silicic Magmatism in Continental Rift Settings:A Petrogeochemical Study of Carboniferous Post-collisional Rift Silicic Volcanics in Tianshan,NW China显示文摘Petrogeochemical data are reported for silicic volcanic rocks from the Tianshan Carboniferous rift, with the aim of discussing the petrogenesis of silicic magmas. Incompatible element vs. incompatible element diagrams display smooth positive trends for the Tianshan Carboniferous rift-related volcanic rocks; the isotope ratios of the silicic lavas [87Sr/86Sr(t)=0.69988–0.70532; εNd(t)=4.76–8.00; 206Pb/204Pb(t)=17.435–18.017; 207Pb/204Pb(t)=15.438–15.509; 208Pb/204Pb(t) = 37.075–37.723] encompass those of the basic lavas. These data suggest a genetic link between rhyolites and basalts, but are not definitive in establishing whether silicic rocks are related to basalts through fractional crystallization or partial melting. Geochemical modeling of incompatible vs. compatible elements excludes the possibility that silicic melts are generated by the melting of basaltic rocks, and indicates a derivation by fractional crystallization plus moderate assimilation of wall rocks (AFC) starting from intermediate rocks to silicic rocks. Continuous AFC from basalt to rhyolite, with small rates of crustal assimilation, best explains the geochemical data. The presence or absence of bimodal volcanism (the “Daly Gap”) might be related to cooling rates of magma chambers. In central and eastern Tianshan, the crust was thinner and the cooling rates of the magma chamber within the crust were greater. These conditions resulted in a rapid fall in temperature within the magma reservoir and caused a narrow temperature interval over which intermediate melts formed, effectively reducing the volume of the intermediate melts. | XIA Linqi XIA Zuchun XU Xueyi LI Xiangmin MA Zhongping WANG Lishe | 2005 | Acta Geologica Sinica(English Edition)2005，79，5： | 14 |
| 4 | Phosphorylcholine polymer nanocapsules prolong the circulation time and reduce the immunogenicity of therapeutic proteins显示文摘蛋白质治疗,治疗学的蛋白质在那里被交付对待混乱,为治疗疾病被认为最安全、很直接的途径。然而,它的应用被有效策略的少量高度为在他们的管理以后在 vivo 交付蛋白质和治疗学的蛋白质的快速的清理限制。这里,我们表明能显著地延长治疗学的蛋白质的发行量时间以及最小化他们的 immunogenicity 的新奇策略。这被与抵抗蛋白质吸附的 crosslinked phosphorylcholine 聚合物的薄层包含单个蛋白质分子完成。通过广泛的细胞的研究,我们证明 crosslinked phosphorylcholine 聚合物壳有效地阻止包含的蛋白质是由巨噬细胞的 phagocytosed,它在 vivo 在 nanoparticles 的清理起一个必要作用。而且,聚合物壳阻止包含的蛋白质被有免疫力的房间识别。作为结果,对治疗学的蛋白质的有免疫力的回答有效地被压制。这个工作描述一个可行方法延长循环时间并且减少治疗学的蛋白质的 immunogenicity,它可以在多样的疾病的治疗支持新奇蛋白质治疗的发展和申请。 | Sheng Liang Yang Liu Xin Jin Gan Liu Jing Wen Linlin Zhang Jie Li Xubo Yuan Irvin S.Y. Chen Wei Chen Hui Wang Linqi Shi Xinyuan Zhu Yunfeng Lu | 2016 | Nano Research2016，9，4： | 11 |
| 5 | Broadly neutralizing antibodies and vaccine design against HIV-1 infection显示文摘Remarkable progress has been achieved for prophylactic and therapeutic interventions against human immunodeficiency virus type I(HIV-1)through antiretroviral therapy.However,vaccine development has remained challenging.Recent discoveries in broadly neutralizing monoclonal antibodies(bNAbs)has led to the development of multiple novel vaccine approaches for inducing bNAbs-like antibody response.Structural and dynamic studies revealed several vulnerable sites and states of the HIV-1 envelop glycoprotein(Env)during infection.Our review aims to highlight these discoveries and rejuvenate our endeavor in HIV-1 vaccine design and development. | Qian Wang Linqi Zhang | 2020 | Frontiers of Medicine2020，14，1： | 4 |
| 6 | Structural basis for bivalent binding and inhibition of SARS-CoV-2 infection by human potent neutralizing antibodies显示文摘Neutralizing monoclonal antibodies(nAbs)to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)represent promising candidates for clinical intervention against coronavirus disease 2019(COVID-19).We isolated a large number of nAbs from SARS-CoV-2-infected individuals capable of disrupting proper interaction between the receptor binding domain(RBD)of the viral spike(S)protein and the receptor angiotensin converting enzyme 2(ACE2).However,the structural basis for their potent neutralizing activity remains unclear.Here,we report cryo-EM structures of the ten most potent nAbs in their native full-length IgG-form or in both IgG-form and Fab-form bound to the trimeric S protein of SARS-CoV-2.The bivalent binding of the full-length IgG is found to associate with more RBDs in the“up”conformation than the monovalent binding of Fab,perhaps contributing to the enhanced neutralizing activity of IgG and triggering more shedding of the S1 subunit from the S protein.Comparison of a large number of nAbs identified common and unique structural features associated with their potent neutralizing activities.This work provides a structural basis for further understanding the mechanism of nAbs,especially through revealing the bivalent binding and its correlation with more potent neutralization and the shedding of S1 subunit. | Renhong Yan Ruoke Wang Bin Ju Jinfang Yu Yuanyuan Zhang Nan Liu Jia Wang Qi Zhang Peng Chen Bing Zhou Yaning Li Yaping Shen Shuyuan Zhang Long Tian Yingying Guo Lu Xia Xinyue Zhong Lin Cheng Xiangyang Ge Juanjuan Zhao Hong-Wei Wang Xinquan Wang Zheng Zhang Linqi Zhang Qiang Zhou | 2021 | Cell Research2021，31，5： | 4 |
| 7 | Biomedical polymers: synthesis, properties, and applications显示文摘Biomedical polymers have been extensively developed for promising applications in a lot of biomedical fields, such as therapeutic medicine delivery, disease detection and diagnosis, biosensing, regenerative medicine, and disease treatment. In this review, we summarize the most recent advances in the synthesis and application of biomedical polymers, and discuss the comprehensive understanding of their property-function relationship for corresponding biomedical applications. In particular, a few burgeoning bioactive polymers, such as peptide/biomembrane/microorganism/cell-based biomedical polymers, are also introduced and highlighted as the emerging biomaterials for cancer precision therapy. Furthermore, the foreseeable challenges and outlook of the development of more efficient, healthier and safer biomedical polymers are discussed. We wish this systemic and comprehensive review on highlighting frontier progress of biomedical polymers could inspire and promote new breakthrough in fundamental research and clinical translation. | Wei-Hai Chen Qi-Wen Chen Qian Chen Chunyan Cui Shun Duan Yongyuan Kang Yang Liu Yun Liu Wali Muhammad Shiqun Shao Chengqiang Tang Jinqiang Wang Lei Wang Meng-Hua Xiong Lichen Yin Kuo Zhang Zhanzhan Zhang Xu Zhen Jun Feng Changyou Gao Zhen Gu Chaoliang He Jian Ji Xiqun Jiang Wenguang Liu Zhuang Liu Huisheng Peng Youqing Shen Linqi Shi Xuemei Sun Hao Wang Jun Wang Haihua Xiao Fu-Jian Xu Zhiyuan Zhong Xian-Zheng Zhang Xuesi Chen | 2022 | Science China Chemistry2022，65，6： | 2 |
| 8 | Both structure and function of human monoclonal antibodies contribute to enhancement of Zika virus infectivity in vitro显示文摘Dear Editor,Antibody-dependent enhancement(ADE)has long been recognized for dengue virus(DENV)in vitro and in vivo.It is now clear that antibodies to DENV can also enhance Zika virus(ZIKV)infection in vitro,and vice versa(Dejnirattisai et al.,2016;Stettler et al.,2016).The characteristics of enhancing antibodies,however,remain elusive.Some have suggested that non-neutralizing antibodies or antibodies recognizing specific antigenic sites are | Min Li Xin Wang Qihui Wang Lei Yu Lanfeng Wang Jinghua Yan Fuchun Zhang Linqi Zhang George F.Gao Xia Jin | 2017 | Science China(Life Sciences)2017，60，12： | 2 |
| 9 | Auto-ignition of biomass synthesis gas in shock tube at elevated temperature and pressure显示文摘Ignition delay times of multi-component biomass synthesis gas(bio-syngas) diluted in argon were measured in a shock tube at elevated pressure(5, 10 and 15 bar, 1 bar = 105Pa), wide temperature ranges(1,100–1,700 K) and various equivalence ratios(0.5, 1.0, 2.0). Additionally, the effects of the variations of main constituents(H2:CO = 0.125–8) on ignition delays were investigated. The experimental results indicated that the ignition delay decreases as the pressure increases above certain temperature(around 1,200 K) and vice versa. The ignition delays were also found to rise as CO concentration increases, which is in good agreement with the literature. In addition, the ignition delays of bio-syngas were found increasing as the equivalence ratio rises. This behavior was primarily discussed in present work. Experimental results were also compared with numerical predictions of multiple chemical kinetic mechanisms and Li's mechanism was found having the best accuracy. The logarithmic ignition delays were found nonlinearly decrease with the H2 concentration under various conditions, and the effects of temperature,equivalence ratio and H2 concentration on the ignition delays are all remarkable. However, the effect of pressure is relatively smaller under current conditions. Sensitivity analysis and reaction pathway analysis of methane showed that R1(H ? O2= O ? OH) is the most sensitive reaction promoting ignition and R13(H ? O2(?M) = HO2(?M)), R53(CH3? H(?M) = CH4(?M)), R54(CH4? H =CH3? H2) as well as R56(CH4? OH = CH3? H2O) are key reactions prohibiting ignition under current experimental conditions. Among them, R53(CH3? H(?M) = CH4(?M)), R54(CH4? H = CH3? H2) have the largest positive sensitivities and the high contribution rate in rich mixture.The rate of production(ROP) of OH of R1 showed that OH ROP of R1 decreases sharply as the mixture turns rich.Therefore, the ignition delays become longer as the equivalence ratio increases. | Linqi Ouyang Hua Li Shuzhou Sun Xiaole Wang Xingcai Lu | 2015 | Science Bulletin2015，60，22： | 2 |
| 10 | Tailoring spin mixtures by ion-enhanced Maxwell magnetic coupling in colortunable organic electroluminescent devices显示文摘In this work,we show that the spin dynamics of excitons can be dramatically altered by Maxwell magnetic field coupling,together with an ion-enhanced,low-internal-splitting-energy organic semiconducting emitter.By employing a unique,alternating current(AC)-driven organic electroluminescent(OEL)device architecture that optimizes this magnetic field coupling,almost complete control over the singlet-to-triplet ratio(from fluorescent to phosphorescent emission in a single device)is realized.We attribute this spin population control to magnetically sensitive polaron–spin pair intersystem crossings(ISCs)that can be directly manipulated through external driving conditions.As an illustration of the utility of this approach to spin-tailoring,we demonstrate a simple hybrid(double-layer)fluorescence–phosphorescence(F–P)device using a polyfluorene-based emitter with a strong external Zeeman effect and ion-induced long carrier diffusion.Remarkable control over de-excitation pathways is achieved by controlling the device-driving frequency,resulting in complete emission blue–red color tunability.Picosecond photoluminescence(PL)spectroscopy directly confirms that this color control derives from the magnetic manipulation of the singlet-totriplet ratios.These results may pave the way to far more exotic organic devices with magnetic-field-coupled organic systems that are poised to usher in an era of dynamic spintronics at room temperature. | Junwei Xu Yue Cui Gregory M.Smith Peiyun Li Chaochao Dun Linqi Shao Yang Guo Hongzhi Wang Yonghua Chen David L.Carroll | 2018 | Light(Science & Applications)2018，7，1： | 2 |
| 11 | Cryptococcus neoformans,a global threat to human health显示文摘Background Emerging fungal pathogens pose important threats to global public health. The World Health Organization has responded to the rising threat of traditionally neglected fungal infections by developing a Fungal Priority Pathogens List (FPPL). Taking the highest-ranked fungal pathogen in the FPPL,Cryptococcus neoformans, as a paradigm, we review progress made over the past two decades on its global burden, its clinical manifestation and management of cryptococcal infection, and its antifungal resistance. The purpose of this review is to drive research efforts to improve future diagnoses, therapies, and interventions associated with fungal infections.Methods We first reviewed trends in the global burden of HIV-associated cryptococcal infection, mainly based on a series of systematic studies. We next conducted scoping reviews in accordance with the guidelines described in the Preferred Reporting Items for Systematic Reviews and Meta-analyses extension for Scoping Reviews using PubMed and ScienceDirect with the keywordCryptococcus neoformans to identify case reports of cryptococcal infections published since 2000. We then reviewed recent updates on the diagnosis and antifungal treatment of cryptococcal infections. Finally, we summarized knowledge regarding the resistance and tolerance ofC. neoformans to approved antifungal drugs.Results There has been a general reduction in the estimated global burden of HIV-associated cryptococcal meningitis since 2009, probably due to improvements in highly active antiretroviral therapies. However, cryptococcal meningitis still accounts for 19% of AIDS-related deaths annually. The incidences of CM in Europe and North America and the Latin America region have increased by approximately two-fold since 2009, while other regions showed either reduced or stable numbers of cases. Unfortunately, diagnostic and treatment options for cryptococcal infections are limited, and emerging antifungal resistance exacerbates the public health burden.Conclusions The rising threat ofC. neoformans is compounded by accumulating evidence for its ability to infect immunocompetent individuals and the emergence of antifungal-resistant variants. Emphasis should be placed on further understanding the mechanisms of pathogenicity and of antifungal resistance and tolerance. The development of novel management strategies through the identification of new drug targets and the discovery and optimization of new and existing diagnostics and therapeutics are key to reducing the health burden. | Youbao Zhao Leixin Ye Fujie Zhao Lanyue Zhang Zhenguo Lu Tianxin Chu Siyu Wang Zhanxiang Liu Yukai Sun Min Chen Guojian Liao Chen Ding Yingchun Xu Wanqing Liao Linqi Wang | 2023 | Infectious Diseases of Poverty2023，12，2： | 2 |
| 12 | Calixarene-integrated nano-drug delivery system for tumortargeted delivery and tracking of anti-cancer drugsin vivo显示文摘Nano-drug delivery systems(nanoDDS)have been extensively investigated clinically to improve the therapeutic effect of anticancer drugs.However,the complicated synthesis during the preparation as well as the potential drug leakage during transportation has greatly limited their general application.In this work,a calixarene-integrated nanoDDS(CanD)that achieves tumor-targeted delivery and tracking of anti-cancer drugs in vivo is presented.The hypoxia-responsive calixarene(SAC4A)exhibits high binding affinity to a series of anti-cancer drugs and rhodamine B(RhB)under normoxic condition while decreasing the binding affinity under hypoxic condition,which leads to the drug release and fluorescence recovery simultaneously.Furthermore,the hypoxia-responsiveness of SAC4A conveys CanD with tumor-targeting ability,resulting in the enrichment of the drug in tumors and enhancement in tumor suppression in mice.Moreover,CanD could become a general platform allowing the delivery of a wide scope of anti-cancer drugs that have strong host-guest interaction with SAC4A. | Lina Xu Jingshan Chai Ying Wang Xinzhi Zhao Dong-Sheng Guo Linqi Shi Zhanzhan Zhang Yang Liu | 2022 | Nano Research2022，15，8： | 1 |
| 13 | SCO3129,a TetR family regulator,is responsible for osmotic stress in Streptomyces coelicolor显示文摘Streptomyces are the soil-dwelling bacteria with a complex lifecycle and a considerable ability to produce a variety of secondary metabolites.Osmoregulation is important for their lifecycle in nature.In the genome of Streptomyces coelicolor M145,SCO3128(encodes a putative fatty acid desaturase),SCO3129(encodes a putative TetR family regulator)and SCO3130(encodes a putative L-carnitine dehydratase)constitute a transcriptional unit,and its transcript was found to be in response to osmotic stress.Disruption of SCO3130 led to a bald phenotype on MMG medium and the mycelia lysis on the edge of the colony when KCl/NaCl was added to the medium.These results indicated that SCO3130 is important for the osmotic stress resistance in S.coelicolor.Transcriptional analysis and electrophoretic mobility shift assays(EMSA)demonstrated that SCO3129 repressed the transcription of SCO3128-3130 operon through directly binding to the promoter region of SCO3128,indicating that SCO3129 regulates the transcription of SCO3128-3130 in response to osmotic stress. | Xihong He Hong Li Yuanyuan Pan Linqi Wang Huarong Tan Gang Liu | 2018 | Synthetic and Systems Biotechnology2018，3，4： | 1 |
| 14 | Stapled peptide-based membrane fusion inhibitors of hepatitis C virus显示文摘 | Hong-Kui Cui Jie Qing Ye Guo Yu-Jia Wang Li-Jia Cui Tian-Hua He Linqi Zhang Lei Liu | 2013 | Bioorganic & Medicinal Chemistry2013，，12： | 1 |
| 15 | A cyclin protein governs the infectious and sexual life cycles of Cryptococcus neoformans显示文摘Cell cycle is a fundamental process underlying growth and development in evolutionarily diverse organisms, including fungi. In human fungal pathogens, cell cycle control generally determines their life cycles, either in the environment or during infections.Thus, cell cycle components can potentially serve as important targets for the development of antifungal strategy against fungal infections. Here, in Cryptococcus neoformans, the most common cause of fatal fungal meningitis, we show that a previously uncharacterized B-type cyclin named Cbc1 is essential for both its infectious and sexual cycles. We reveal that Cbc1 coordinates various sexual differentiation and molecular processes, including meiosis. Especially, the absence of Cbc1 abolishes formation of sexual spores in C. neoformans, which are presumed infectious particles. Cbc1 is also required for the major Cryptococcus pathogenic attributes. Virulence assessment using the murine model of cryptococcosis revealed that the cbc1 mutant is avirulent.Together, our results provide an important insight into how C. neoformans employs shared cell cycle regulation to coordinate its infectious and sexual cycles, which are considered crucial for virulence evolution and the production of infectious spores. | Pengjie Hu Linxia Liu Weixin Ke Xiuyun Tian Linqi Wang | 2021 | Science China(Life Sciences)2021，64，8： | 1 |
| 16 | In-biofilm generation of nitric oxide using a magnetically-targetable cascade-reaction container for eradication of infectious biofilms显示文摘Cascade-reaction chemistry can generate reactive-oxygen-species that can be used for the eradication of infectious biofilms.However,suitable and sufficient oxygen sources are not always available near an infection site,while the reactive-oxygen-species generated are short-lived.Therefore,we developed a magnetic cascade-reaction container composed of mesoporous Fe_(3)O_(4)@SiO_(2) nanoparticles containing glucose-oxidase and L-arginine for generation of reactive-oxygen-species.Glucose-oxidase was conjugated with APTES facilitating coupling to Fe_(3)O_(4)@SiO_(2) nanoparticles and generation of H_(2)O_(2) from glucose.L-arginine was loaded into the nanoparticles to generate NO from the H_(2)O_(2) generated.Using an externally-applied magnetic field,cascade-reaction containers could be homogeneously distributed across the depth of an infectious biofilm.Cascade-reaction containers with coupled glucose-oxidase were effective in killing planktonic,Gram-positive and Gram-negative bacteria.Additional efficacy of the L-arginine based second cascade-reaction was only observed when H_(2)O_(2) as well as NO were generated in-biofilm.In vivo accumulation of cascade-reaction containers inside abdominal Staphylococcus aureus biofilms upon magnetic targeting was observed real-time in living mice through an implanted,intra-vital window.Moreover,vancomycin-resistant,abdominal S.aureus biofilms could be eradicated consuming solely endogenous glucose,without any glucose addition.Herewith,a new,non-antibiotic-based infection-control strategy has been provided,constituting a welcome addendum to the shrinking clinical armamentarium to control antibiotic-resistant bacterial infections. | Guang Yang Da-Yuan Wang Yong Liu Fan Huang Shuang Tian Yijin Ren Jianfeng Liu Yingli An Henny C.van der Mei Henk J.Busscher Linqi Shi | 2022 | Bioactive Materials2022，7，8： | 0 |
| 17 | Internet of Cultural Things: Current Research, Challenges and Opportunities显示文摘Driven by the visions of the Internet of Things(IoT),Artificial Intelligence(AI),and 5G communications,the Internet of Cultural Things(IoCT)realize the comprehensive interconnection among cultural products,cultural services,cultural resources,and cultural platforms,bringing individuals with richer humanistic experience,increasing economic benefits for the cultural sector,and promoting the development of cultural heritage protection and education.At present,IoCT has received widespread attention in both industry and academia.To explore new research opportunities and assist users in constructing suitable IoCT systems for specific applications,this survey provides a comprehensive overview of the IoCT components and key technologies.A comparison study of representative IoCT systems is presented according to their applicability.A general platform architecture of IoCT is proposed to link cultural objects with the internet and human.Finally,open issues for research challenges and future opportunities of IoCT are also studied in this paper. | Xiaoting Liang Fang Liu Linqi Wang Baoying Zheng Yiyuan Sun | 2023 | Computers, Materials & Continua2023，，1： | 0 |
| 18 | Non-cytotoxic nanoparticles re-educating macrophages achieving both innate and adaptive immune responses for tumor therapy显示文摘Macrophages are important antigen-presenting cells to combat tumor via both innate and adaptive immunity,while they are programmed toM2 phenotype in established tumors and instead promote cancer development and metastasis.Here,we develop a nanomedicine that can re-educate M2 polarized macrophages to restore their anti-tumor activities.The nanomedicine has a core-shell structure to co-load IPI549,a PI3Kγinhibitor,and CpG,a Toll-like receptor 9 agonist.Specifically,the hydrophobic IPI549 is self-assembled into a pure drug nano-core,while MOF shell layer is coated for CpG encapsulation,achieving extra-high total drugs loading of 44%.Such nanosystem could facilitate intracellular delivery of the payloads but without any cytotoxicity,displaying excellent biocompatibility.After entering macrophages,the released IPI549 and CpG exert a synergistic effect to switch macrophages from M2 to M1 phenotype,which enables anti-tumor activities via directly engulfing tumor cells or excreting tumor killing cytokines.Moreover,tumor antigens released from the dying tumor cells could be effectively presented by the re-educated macrophages owing to the up-regulation of various antigen presenting mediators,resulting in infiltration and activation of cytotoxic T lymphocytes.As a result,the nanosystem triggers a robust antitumor immune response in combination with PD-L1 antibody to inhibit tumor growth and metastasis.This work provides a non-cytotoxic nanomedicine to modulate tumor immune microenvironment by reprograming macrophages. | Shengmei Wang Xuanjun Liu Minghua Yang Linqi Ouyang Jinsong Ding Shengfeng Wang Wenhu Zhou | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，4： | 0 |
| 19 | Effects of rotational culture on morphology,nitric oxide production and cell cycle of endothelial cells显示文摘Devices for the rotational culture of cells and the study of biological reactions have been widely applied in tissue engineering.However,there are few reports exploring the effects of rotational culture on cell morphology,nitric oxide(NO)production,and cell cycle of the endothelial cells from human umbili-cal vein on the stent surface.This study focuses on these parameters after the cells are seeded on the stents.Results showed that covering of stents by endothelial cells was improved by rotational culture.NO produc-tion decreased within 24 h in both rotational and static culture groups.In addition,rotational culture signifi-cantly increased NO production by 37.9%at 36 h and 28.9%at 48 h compared with static culture.Flow cytometry showed that the cell cycle was not obviously influenced by rotational culture.Results indicate that rotational culture may be helpful for preparation of cell-seeded vascular grafts and intravascular stents,which are expected to be the most frequently implanted materials in the future. | CHAOJUN TANG XUE WU LINQI YE XIANG XIE GUIXUE WANG | 2012 | BIOCELL2012，36，3： | 0 |
| 20 | An Exceptional Broad-Spectrum Nanobiocide for Multimodal and Synergistic Inactivation of Drug-Resistant Bacteria显示文摘This study reports the fabrication of a novel photothermal material formed via the physical blending of excess lauric acid(LA)and cupric acetate,followed by efficient ligand exchange.Surprisingly,the copper–LA complex exhibited a 12-fold enhancement of the molar extinction coefficient in the nearinfrared(NIR)region relative to aqueous cupric acetate.Inspired by this interesting finding,we formulated these photothermal materials into colloidally dispersed nanoparticles via a technique that combined nanoprecipitation and in situ surface polymerization for antibacterial studies.The resultant nanoparticles exhibited rapid and stable photothermal responses to NIR irradiation,with a 4-fold enhanced photothermal conversion efficiency relative to aqueous cupric acetate.Since a positively charged monomer was incorporated during in situ surface polymerization,these positively charged nanoparticles were ingested efficiently and subsequently digested by drug-resistant bacteria.By combining the LA-mediated membrane-damaging effect,copper-mediated Fenton-like reaction,as well as the photothermal effect of the copper–LA complex,a broad-spectrum,multimodal,and synergistic antibacterial effect was achieved both in vitro and in vivo,with the killing efficiency up to 99.99%for ampicillin-resistant Escherichia coli(Ampr E.coli)and 99.9999%for methicillinresistant Staphylococcus aureus(MRSA).Our newly developed nanobiocide represents a class of exceptional broad-spectrum antibacterial materials,holding great potential for treating drug-resistant infections in clinical settings. | Ke Xue Cuihong Yang Chun Wang Yang Liu Jianfeng Liu Linqi Shi Chunlei Zhu | 2022 | CCS Chemistry2022，4，1： | 0 |
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