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45篇 您的检索式:作者名="Lin Jinxing"
    题名 作者 年代 出处 被引量
1Anatomical and chemical characteristics associated with lodging resistance in wheat显示文摘Anatomical and chemical characteristics of stems affect lodging in wheat(Triticum aestivum L.) cultivars. Traits associated with lodging resistance, such as plant height, stem strength, culm wall thickness, pith diameter, and stem diameter, were extensively investigated in earlier studies. However, the solid stem trait was rarely considered. In this study, we measured a range of anatomical and chemical characteristics on solid and hollow stemmed wheat cultivars. Significant correlations were detected between resistance to lodging and several anatomical features, including width of mechanical tissue, weight of low internodes, and width of stem walls. Morphological features that gave the best indication of improved lodging resistance were increased stem width, width of mechanical tissue layer, and stem density. Multiple linear regression analysis showed that 99% of the variation in lodging resistance could be explained by the width of the mechanical tissue layer, suggesting that solid stemmed wheat has several anatomical features for increasing resistance to lodging. In addition, microsatellite markers GWM247 and GWM340 were linked to a single solid stem QTL on chromosome 3BL in a population derived from the cross Xinongshixin(solid stem)/Line 3159(hollow stem). These markers should be valuable in breeding wheat for solid stem.Eryan Kong Dongcheng Liu Xiaoli Guo Wenlong Yang Jiazhu Sun Xin Li Kehui Zhan Dangqun Cui Jinxing Lin Aimin Zhang 2013The Crop Journal2013,1,1:43
2Effects of stem structure andcell wall components on bending strength in wheat显示文摘Morphological traits, anatomical features, chemical components and bending stress in the stems of three genotypes of wheat (Triticum aestivum L.), namely Xiaoyan54, 8602 and Xiaoyan81, were ex- amined by means of light microscopy coupled with Fourier transform infrared spectroscopy (FTIR). No- ticeable changes in morphological and anatomical traits were observed, including outer radius of stem, the ratio of stem outer radius to stem wall thickness, various tissue proportions and variations among dif- ferent types of vascular bundles. The results of chemical analysis revealed that Xiaoyan81 had the highest cellulose content in comparison with Xiao- yan54 and 8602, whereas lignin level in Xiaoyan81 was lower than that in 8602 but higher that that in Xiaoyan54. Bending stress analysis demonstrated that Xiaoyan81 may be the main target for identifica- tion, for it had the highest bending stress among the stems of three genotypes. Associated with bending stress, all the results presented here suggested that the ratio of stem wall thickness to its outer radius, schlerenchyma tissue proportion, the average num- ber of big VB per unit and the cellulose content are four important factors affecting the mechanical strength of Xiaoyan81 wheat stems, which can be considered as the key parameters for selecting varie- ties with bending stress. Therefore, it was suggested that in the selection of lodging resistant cultivars one should consider those characterized with large ratio of outer radius of stem to stem wall thickness, great-erschlerenchyma tissue proportion, high average number of big VB per unit with high cellulose content in their stems.WANG Jian ZHU Jinmao LIN Qinqin LI Xiaojuan TENG Nianjun LI Zhensheng LI Bin ZHANG Aimin LIN Jinxing 2006Chinese Science Bulletin2006,51,7:30
3Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells显示文摘Arabidopsis BRASSINOSTEROID INSENSITIVE1 (BRI1 ) 的主要 brassinosteroid (BR ) 受体在 BR 发信号,而是在 BRI1 成为主观和集会状态遗体上位于 BR 的效果下面的分子的机制起基本作用不清楚。这里,我们使用了可变角度总数内部思考荧光显微镜学和荧光分析动力学的跨关联的光谱学标注 ofGFP 的 BRI1。我们发现了那,响应 BR,有 AtFlot1-mCherry 的 BRI1-GFP 的合作本地化的度增加了,并且特别 BR 刺激了膜 BRI1 成为主观的联系 microdomain 的小径。我们也在 endocytosis 有缺点的 chc2-1 异种和 AtFlot1 amiRNA 15-5 线验证了这些观察。而且, bri1-EMS-suppressor 的 phosphorylation 地位的检查 BR 应答的基因表示的第 1a 测量表明膜 microdomains 影响 BR 发信号。这些结果 suggestthat BR 支持划分 BRI1 成功能的膜 microdomains 激活 BR 发信号。Li Wang Hong Li Xueqin Lv Tong Chen Ruili Li Yiqun xuei Jianjun Jiang Biao Jin Frantisek Baluska Jozef Samaj Xuelu Wang Jinxing Lin 2015Molecular Plant2015,8,9:11
4Techniques for detecting protein-protein interactions in living cells:principles, limitations, and recent progress显示文摘Detecting protein-protein interactions(PPIs) provides fundamental information for understanding biochemical processes such as the transduction of signals from one cellular location to another; however, traditional biochemical techniques cannot provide sufficient spatio-temporal information to elucidate these molecular interactions in living cells. Over the past decade, several new techniques have enabled the identification and characterization of PPIs. In this review, we summarize three main techniques for detecting PPIs in vivo, focusing on their basic principles and applications in biological studies. We place a special emphasis on their advantages and limitations, and, in particular, we introduced some uncommon new techniques, such as single-molecule FRET(smFRET), FRET-fluorescence lifetime imaging microscopy(FRET-FLIM), cytoskeleton-based assay for protein-protein interaction(CAPPI) and single-molecule protein proximity index(smPPI), highlighting recent improvements to the established techniques. We hope that this review will provide a valuable reference to enable researchers to select the most appropriate technique for detecting PPIs.Yaning Cui Xi Zhang Meng Yu Yingfang Zhu Jingjing Xing Jinxing Lin 2019Science China(Life Sciences)2019,62,5:9
5MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar显示文摘Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants.Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang Zhenying Wu HanWang LeiWang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu 2020Horticulture Research2020,7,1:9
6Relationship between stomatal density and the changes of atmospheric CO_2 concentrations显示文摘The relationship between the stomatal density of five woody plants endemic to China, i.e. Eucommia ulmoides, Quercus liaotungensis, Q. glandulifera var. brevipetiolata, Cyclocarya paliurus and Ficus heteromorpha, and the atmospheric CO-2 concentrations was studied by observations on leaves of the herbarium-stored specimens(1920s-1990s). The results showed that the stomatal density in Eucommia ulmoides, Quercus liaotungensis and Q. glandulifera% var. brevipetiolata decreased significantly in response to the elevated atmospheric CO-2 concentrations, while in Cyclocarya paliurus it decreased slightly and in Ficus heteromorpha there were no responses.HE Xinqiang, LIN Yuehui, LIN Jinxing and HU YuxiInstitute of Botany, Chinese Academy of Sciences, Beijing, 100093, China 1998Chinese Science Bulletin1998,43,11:7
7An Arabidopsis Class II Formin,AtFH19,Nucleates Actin Assembly,Binds to the Barbed End of Actin Filaments,and Antagonizes the Effect of AtFH1 on Actin Dynamics显示文摘Formin is a major protein responsible for regulating the nucleation of actin filaments,and as such,it permits the cell to control where and when to assemble actin arrays.It is encoded by a multigene family comprising 21 members in Arabidopsis thaliana.The Arabidopsis formins can be separated into two phylogenetically-distinct classes:there are 11 class I formins and 10 class II formins.Significant questions remain unanswered regarding the molecular mechanism of actin nucleation and elongation stimulated by each formin isovariant,and how the different isovariants coordinate to regulate actin dynamics in cells.Here,we characterize a class II formin,AtFH19,biochemically.We found that AtFH19 retains all general properties of the formin family,including nucleation and barbed end capping activity.It can also generate actin filaments from a pool of actin monomers bound to profilin.However,both the nucleation and barbed end capping activities of AtFH19 are less efficient compared to those of another well-characterized formin,AtFH1.Interestingly,AtFH19 FH1FH2 competes with AtFH1 FH1FH2 in binding actin filament barbed ends,and inhibits the effect of AtFH1 FH1FH2 on actin.We thus propose a mechanism in which two quantitatively different formins coordinate to regulate actin dynamics by competing for actin filament barbed ends.Yiyan Zheng Haibo Xin Jinxing Lin Chun-Ming Liu Shanjin Huang 2012Journal of Integrative Plant Biology2012,54,10:7
8Single-Particle Tracking for the Quantification of Membrane Protein Dynamics in Living Plant Cells显示文摘The plasma membrane is a sophisticated,organized,and highly heterogeneous structure that compartmentalizes cellular processes.To decipher the biological processes involving membrane proteins,it is necessary to analyze their spatiotemporal dynamics.However,it is difficult to directly assess the dynamics and interactions of biomolecules in living cells using traditional biochemical methods.Singleparticle tracking (SPT)methods for imaging and tracking single particles conjugated with fluorescent probes offer an ideal approach to acquire valuable and complementary information about dynamic intracellular processes.SPT can be used to quantitatively monitor the diverse motions of individual particles in living cells.SPT also provides super-spatiotemporal resolution that allows early-stage or rapid response information to be obtained for a better understanding of molecular basis of associated signal transduction processes.More importantly,SPT can be used to detect the motion paths of individual biomolecules in vivo and in situ,thus unveiling the dynamic behavior of the biomolecules that support developmental processes in living cells.In this review,we give an overview of SPT methods,from image acquisition to the detection of single particles,as well as tracking and data analysis.We also discuss recent applications of SPT methods in the field ofplant biology to reveal the complex biological functions of membrane proteins.Yaning Cui Meng Yu Xiaomin Yao Jingjing Xing Jinxing Lin Xiaojuan Li 2018Molecular Plant2018,11,11:5
9Atomic force microscopic observation on substructure of pollen exine in Cedrus deodara and Metasequoia glyptostroboides显示文摘The substructure of pollen exine in Cedrus deodara (Roxb.) Loud, and Metasequoia glyptostroboides Hu et Cheng has been examined with an atomic force microscope (AFM). The results indicate that the exine substructure units containing sporopollenin in two species are similar in shape, which are granular, but slightly different in size. In Cedrus the substructure unit of pollen exine appears to be 56-99 nm long and 42-74 nm wide, while in Metasequoia it appears to be 81-118 nm long and 43-98 nm wide. It has been observed that the subunits of pollen exine in Cedrus arranged tightly to form short-rod-like or spheroidal pollen exine units, several or more than ten of which formed an island-like structure. There are various spaces among these island-like structures which are interconnected to occupy the entire pollen exine. In Metasequoia, the subunits of pollen exine also arranged tightly with a distribution tendency of cluster of 3-10, however, no obvious boundary exists among these clusters. From ourXING Shuping LI Bingshi WANG Chen HU Yuxi LIN Jinxing 2000Chinese Science Bulletin2000,45,16:5
10Admissibility analysis and control synthesis for switched linear singular systems显示文摘The problem of admissibility analysis and control synthesis of discrete-time switched linear singular (SLS) systems for arbitrary switching laws is solved.By using the switched Lyapunov function approach,some new sufficient conditions under which the SLS system is admissible for arbitrary switching laws are derived in terms of linear matrix inequalities(LMIs).Based on the admissibility results,control synthesis is then to design switched state feedback and static output feedback controllers,guaranteeing that the resulting closed-loop system is admissible.The presented results can be viewed as the extensions of previous works on switched Lyapunov function approach from the regular switched systems to singular switched cases.Examples are provided to demonstrate the reduced conservatism and effectiveness of the proposed conditions.Lin Jinxing Fei Shumin Shen Jiong 2009Journal of Systems Engineering and Electronics2009,20,5:4
11Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains显示文摘Phototropin (辐透) 调停了发信号由蓝光(BL ) 开始了在在植物在血浆膜(下午) 优化光合的轻俘获起一个关键作用。然而,响应 BL 在 PM 位于辐透活动的规定下面的机制仍然保持大部分不清楚。在这研究,由单个粒子的追踪和 phot1-GFP 蛋白质的逐步的 photobleaching 分析,我们在黑暗 phot1 表明了那 ? 蛋白质留在一个不活跃的状态并且主要作为单体存在。Dimerization 和 phot1-GFP 的散开率响应 BL 以一种剂量依赖者方式增加了。相反, BL 没影响 kinase 不活跃的 phot1 D806N -GFP?but 确实提高了它的 dimerization,建议那 phot1 dimerization 独立于 phosphorylation。F ? rster 回声精力转移荧光一生成像显微镜学 ? 分析表明在 phot1-GFP 和充满甾醇的类脂化合物环境的一个标记之间的相互作用, AtRem1.3-mCherry,与 BL 处理的增加的时间被提高。然而,这个 BL 依赖的相互作用不在植物共同表示是明显的 phot1 D806N -GFP 和 AtRem1.3-mCherry,显示那 BL 便于功能的 phot1-GFP 的 translocation 进标记 AtRem1.3 的 microdomains 激活调停辐透的发信号。相反地,甾醇弄空稀释了 phot1-GFP 动力学, dimerization,和 phosphorylation。一起拿,这些结果显示膜 microdomains 充当在 PM 组织为激活的 phot1 的合适的功能必要的平台。Yiqun Xue Jingjing Xing Yinglang Wan Xueqin Lv Lusheng Fan Yongdeng Zhang Kai Song Li Wang Xiaohua Wang Xin Deng Frantisek Baluska John M. Christie Jinxing Lin 2018Molecular Plant2018,11,6:4
12An initial discussion on major controlling factors on formation of coal-formed large-medium sas fields显示文摘The so-called 'large-medium gas field' means a gas field with proven reserves equal to or larger than 100×108 m3. Up to 1997, 23 coal-formed great-medium fields are discovered in China, excluding two in Taiwan Province. Their proven reserves take 50.9% in total natural gas reserves. Therefore, the coal-formed great-medium gas field plays a decisive role in natural gas reserves and the study of their controlling factors is very important both in theory and in practice. There are six major controlling factors on the formation of coal-formed large-medium gas fields: ( Ⅰ) The generating center of coal-formed gas and its surrounding area; (Ⅱ) low potential area; (Ⅲ) late pool-forming period; (Ⅳ) traps related to the paleo-uplifits in coal-formed gas area; (Ⅴ) above or between abnormally pressured compartments; (Ⅵ) faulted traps related to coal-bed.DAI Jinxing, XIA Xinyu, HONG Feng, ZHAO Lin, SUN Dongmin & SHI XinResearch Institute of Petroleum Exploration and Development, Beijing 100083, China Correspondence should be addressed to Dai Jinxing 2000Chinese Science Bulletin2000,45,5:4
13Three-dimensional reconstruction of Picea wilsonii Mast. pollen grains using automated electron microscopy显示文摘Three-dimensional electron microscopy(3 D-EM) has attracted considerable attention because of its ability to provide detailed information with respect to developmental analysis. However, large-scale high-resolution 3 D reconstruction of biological samples remains challenging. Herein, we present a 3 D view of a Picea wilsonii Mast. pollen grain with 100 nm axial and38.57 nm lateral resolution using AutoCUTS-SEM(automatic collector of ultrathin sections-scanning electron microscopy). We established a library of 3,127 100 nm thick serial sections of pollen grains for preservation and observation, demonstrating that the protocol can be used to analyze large-volume samples. After obtaining the SEM images, we reconstructed an entire pollen grain comprising 734 serial sections. The images produced by 3D reconstruction clearly revealed the main components of the P.wilsonii pollen grain, i.e., two sacci and pollen corpus, tube cell, generative cell, and two degenerated prothallial cells, and their internal organization. In addition, we performed a quantitative analysis of the different pollen grain cells, including sacci, and found that there were 202 connections within a saccus SEM image. Thus, for the first time, this study provided a global 3D view of the entire pollen grain, which will be useful for analyzing pollen development and growth.Weiwei Shen Lingyu Ma Xi Zhang Xixia Li Yuanyuan Zhao Yanping Jing Yun Feng Xueke Tan Fei Sun Jinxing Lin 2020Science China(Life Sciences)2020,63,2:3
14Penta-P2X (X=C, Si) monolayers as wide-bandgap semiconductors: A first principles prediction显示文摘Mosayeb Naseri Shiru Lin Jaafar Jalilian Jinxing Gu Zhongfang Chen 2018Frontiers of physics2018,13,3:3
15Plant multiscale networks:charting plant connectivity by multi-level analysis and imaging techniques显示文摘In multicellular and even single-celled organisms,individual components are interconnected at multiscale levels to produce enormously complex biological networks that help these systems maintain homeostasis for development and environmental adaptation.Systems biology studies initially adopted network analysis to explore how relationships between individual components give rise to complex biological processes.Network analysis has been applied to dissect the complex connectivity of mammalian brains across different scales in time and space in The Human Brain Project.In plant science,network analysis has similarly been applied to study the connectivity of plant components at the molecular,subcellular,cellular,organic,and organism levels.Analysis of these multiscale networks contributes to our understanding of how genotype determines phenotype.In this review,we summarized the theoretical framework of plant multiscale networks and introduced studies investigating plant networks by various experimental and computational modalities.We next discussed the currently available analytic methodologies and multi-level imaging techniques used to map multiscale networks in plants.Finally,we highlighted some of the technical challenges and key questions remaining to be addressed in this emerging field.Xi Zhang Yi Man Xiaohong Zhuang Jinbo Shen Yi Zhang Yaning Cui Meng Yu Jingjing Xing Guangchao Wang Na Lian Zijian Hu Lingyu Ma Weiwei Shen Shunyao Yang Huimin Xu Jiahui Bian Yanping Jing Xiaojuan Li Ruili Li Tonglin Mao Yuling Jiao Sodmergen Haiyun Ren Jinxing Lin 2021Science China(Life Sciences)2021,64,9:3
16Regulation of cytoskeleton-associated protein activities: Linking cellular signals to plant cytoskeletal function显示文摘The plant cytoskeleton undergoes dynamic remodeling in response to diverse developmental and environmental cues. Remodeling of the cytoskeleton coordinates growth in plant cells, including trafficking and exocytosis of membrane and wall components during cell expansion, and regulation of hypocotyl elongation in response to light. Cytoskeletal remodeling also has key functions in disease resistance and abiotic stress responses. Many stimuli result in altered activity of cytoskeleton-associatedproteins,microtubuleassociated proteins(MAPs) and actin-binding proteins(ABPs). MAPs and ABPs are the main players determining the spatiotemporally dynamic nature of the cytoskeleton, functioning in a sensory hub that decodes signals to modulate plant cytoskeletal behavior. Moreover, MAP and ABP activities and levels are precisely regulated during development and environmental responses, but our understanding of this process remains limited. In this review, we summarize the evidence linking multiple signaling pathways, MAP and ABP activities and levels, and cytoskeletal rearrangements in plant cells. We highlight advances in elucidating the multiple mechanisms that regulate MAP and ABP activities and levels, including calcium and calmodulin signaling, ROP GTPase activity, phospholipid signaling, and post-translational modifications.Na Lian Xinwei Wang Yanping Jing Jinxing Lin 2021Journal of Integrative Plant Biology2021,63,1:3
17Robust stability and stabilization for uncertain discrete-time switched singular systems with time-varying delays显示文摘The problems of robust stability and stabilization via memoryless state feedback for a class of discrete-time switched singular systems with time-varying delays and linear fractional uncertainties are investigated.By constructing a novel switched Lyapunov-Krasovskii functional,a delay-dependent criterion for the unforced system to be regular,causal and uniformly asymptotically stable is established in terms of linear matrix inequalities(LMIs).An explicit expression for the desired memoryless state feedback stabilization controller is also given.The merits of the proposed criteria lie in their less conservativeness and relative simplicity,which are achieved by considering additionally useful terms(ignored in previous methods) when estimating the upper bound of the forward difference of the Lyapunov-Krasovskii functional and by avoiding utilizing any model augmentation transformation.Some numerical examples are provided to illustrate the validity of the proposed methods.Jinxing Lin Shumin Fei Jiong Shen 2010Journal of Systems Engineering and Electronics2010,21,4:2
18Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy显示文摘译解适当空间、时间的规模上的蛋白质和类脂化合物分子的动力学可以使蛋白质功能和膜组织清楚些。然而,传统的体积途径不能明白地确定在生活房间的蛋白质的极其多样的活动性和相互作用。荧光关联光谱学(FCS ) 是一种强大的技术描述发生在单个分子的水平并且在毫微秒上到第二 timescales 的事件;因此, FCS 能在膜系统的异构的组织上提供数据。FCS 能也与另外的显微镜学技术被相结合,例如超级决定的技术。更重要地, FCS 是最低限度地侵略的,它成为在开发期间检测关键蛋白质的异构的分发和动力学是一条理想的途径。在这评论,我们关于 FCS 的发展给简短介绍并且在理解膜蛋白质的植物房间膜和动力学和相互作用的组织总结 FCS 的重要贡献。我们也在植物生物学讨论这种技术的潜在的应用。Xiaojuan Li Jingjing Xing Zongbo Qiu Qihua He Jinxing Lin 2016Molecular Plant2016,9,9:2
19THESEUS1 positively modulates plant defense responses against Botrytis cinerea through GUANINE EXCHANGE FACTOR4 signaling显示文摘The plant cell wall is an important interface for sensing pathogen attack and activating signaling pathways that promote plant immune responses.THESEUS1(THE1) acts as a sensor of cell wall integrity that controls cell elongation during plant growth.However, no specific role for THE1 in plant defense responses has been reported. Here, we found that THE1 interacts with GUANINE EXCHANGE FACTOR4(GEF4)and that both proteins play regulatory roles in plant resistance to the necrotrophic fungus Botrytis cinerea.Genetic analysis showed that THE1 and GEF4 function in the same genetic pathway to mediate plant defense responses. In addition, using transcriptome analysis, we identified various genes(such as defense-related,secondary metabolite-related, and transcription factor genes) that are likely downstream targets in the THE1-GEF4 signaling pathway. Our results suggest that THE1 functions as an upstream regulator of GEF4 signaling to positively regulate defense responses against B. cinerea in Arabidopsis.shaofeng qu xi zhang yutong song jinxing lin xiaoyi shan 2017Journal of Integrative Plant Biology2017,59,11:2
20Impulsive controller design for nonlinear networked control systems with time delay and packet dropouts显示文摘The globally exponential stability of nonlinear impulsive networked control systems(NINCS) with time delay and packet dropouts is investigated.By applying Lyapunov function theory,sufficient conditions on the global exponential stability are derived by introducing a comparison system and estimating the corresponding Cauchy matrix.An impulsive controller is explicitly designed to achieve exponential stability and ensure state converge with a given decay rate for the system.The Lorenz oscillator system is presented as a numerical example to illustrate the theoretical results and effectiveness of the proposed controller design procedure.Xianlin Zhao Shumin Fei Jinxing Lin 2012Journal of Systems Engineering and Electronics2012,23,3:2
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