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| 1 | MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions显示文摘在氮(N) 奢侈条件下面的住宿实质地减少庄稼产量和种子质量。然而,植物住宿抵抗的分子的机制仍然保持大部分不清楚,特别在玉米。我们这里报导 ZmmiR528 的表示, monocot 特定的 microRNA,被 N 导致 ? 奢侈但是由 N 缺乏减少了。我们由 thioacidolysis 和 N 奢侈显著地减少的乙酰溴化物分析证明在玉米的木质素以及它的全部的内容的 H, G,和 S 单体的产生射击。我们进一步示威 ? 那 ZmLACCASE3 (ZmLAC3 ) 编码包含铜的 laccases,和 ZmLACCASE5 (ZmLAC5 ) 是 ZmmiR528 的目标。在杂交显示出的 situ, ZmmiR528 是,这主要在玉米表示了脉管的纸巾。显著地增加的 ZmLAC3 的 ZmmiR528 或 overexpression 击倒木质素内容和玉米的树皮透度计抵抗起源。相反,转基因的玉米植物 overexpressing ZmmiR528 减少了木质素内容和树皮透度计抵抗并且对在揭示的 N 奢侈定序 conditions.?RNA 分析下面的住宿敏感那 ZmPAL7 和 ZmPAL8 是在转基因的玉米线 downregulating ZmmiR528 的 upregulated。在 N 奢侈条件下面, ZmPALs 的表示层次比在野类型在 ZmmiR528 击倒的线是高得多的并且 ? 转基因的玉米线 overexpressing ZmmiR528。一起拿,这些结果显示由调整 ZmLAC3 和 ZmLAC5 的表示, ZmmiR528 在 N 奢侈条件下面影响玉米住宿抵抗。 | Qing Sun Xiaogang Liu Juan Yang Wenwen Liu Qingguo Du Hongqiu Wang Chunxiang Fu Wen-Xue Li | 2018 | Molecular Plant2018，11，6： | 21 |
| 2 | MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar显示文摘Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants. | Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang Zhenying Wu HanWang LeiWang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu | 2020 | Horticulture Research2020，7，1： | 9 |
| 3 | The genome of Medicago polymorpha provides insights into its edibility and nutritional value as a vegetable and forage legume显示文摘Medicago polymorpha is a nutritious and palatable forage and vegetable plant that also fixes nitrogen.Here,we reveal the chromosome-scale genome sequence of M.polymorpha using an integrated approach including Illumina,PacBio and Hi-C technologies.We combined PacBio full-length RNA-seq,metabolomic analysis,structural anatomy analysis and related physiological indexes to elucidate the important agronomic traits of M.polymorpha for forage and vegetable usage.The assembled M.polymorpha genome consisted of 457.53Mb with a long scaffold N50 of 57.72Mb,and 92.92%(441.83Mb)of the assembly was assigned to seven pseudochromosomes.Comparative genomic analysis revealed that expansion and contraction of the photosynthesis and lignin biosynthetic gene families,respectively,led to enhancement of nutritious compounds and reduced lignin biosynthesis in M.polymorpha.In addition,we found that several positively selected nitrogen metabolism-related genes were responsible for crude protein biosynthesis.Notably,the metabolomic results revealed that a large number of flavonoids,vitamins,alkaloids,and terpenoids were enriched in M.polymorpha.These results imply that the decreased lignin content but relatively high nutrient content of M.polymorpha enhance its edibility and nutritional value as a forage and vegetable.Our genomic data provide a genetic basis that will accelerate functional genomic and breeding research on M.polymorpha as well as other Medicago and legume plants. | Jiawen Cui Zhaogeng Lu Tianyi Wang Gang Chen Salma Mostafa Hailong Ren Sian Liu Chunxiang Fu Li Wang Yingfang Zhu Jinkai Lu Xiang Chen Zhenwu Wei Biao Jin | 2021 | Horticulture Research2021，8，1： | 6 |
| 4 | Improved Multi-proxy Signature Scheme without Random Oracles显示文摘 | SUN Ying XU Chunxiang WANG Hua FU Chaoxu | 2011 | Chinese Journal of Electronics2011，20，2： | 4 |
| 5 | The nodulation and nyctinastic leaf movement is orchestrated by clock gene LHY in Medicago truncatula显示文摘As sessile organisms,plants perceive,respond,and adapt to the environmental changes for optimal growth and survival.The plant growth and fitness are enhanced by circadian clocks through coordination of numerous biological events.In legume species,nitrogen-fixing root nodules were developed as the plant organs specialized for symbiotic transfer of ni-trogen between microsymbiont and host.Here,we report that the endogenous circadian rhythm in nodules is regulated by MtLHY in legume species Medicago truncatula.Loss of function of MtLHY leads to a reduction in the number of nodules formed,resulting in a diminished ability to assimilate nitrogen.The operation of the 24-h rhythm in shoot is further influenced by the availability of nitrogen produced by the nodules,leading to the irregulated nyctinastic leaf movement and reduced biomass in mtlhy mutants.These data shed new light on the roles of MtLHY in the orchestration of circadian oscillator in nodules and shoots,which provides a mechanistic link between nodulation,nitrogen assimilation,and clock function. | Yiming Kong Lu Han Xiu Liu Hongfeng Wang Lizhu Wen Xiaolin Yu Xiaodong Xu Fanjiang Kong Chunxiang Fu Kirankumar S.Mysore Jiangqi Wen Chuanen Zhou | 2020 | Journal of Integrative Plant Biology2020，62，12： | 4 |
| 6 | Structural and Functional Analyses of Hub Micro RNAs in An Integrated Gene Regulatory Network of Arabidopsis显示文摘MicroRNAs(miRNAs)are trans-acting small regulatory RNAs that work coordinately with transcription factors(TFs)to shape the repertoire of cellular m RNAs available for translation.Despite our growing knowledge of individual plant miRNAs,their global roles in gene regulatory networks remain mostly unassessed.Based on interactions obtained from public databases and curated from the literature,we reconstructed an integrated miRNA network in Arabidopsis that includes 66 core TFs,318 miRNAs,and 1712 downstream genes.We found that miRNAs occupy distinct niches and enrich miRNA-containing feed-forward loops(FFLs),particularly those with miRNAs as intermediate nodes.Further analyses revealed that miRNA-containing FFLs coordinate TFs located in different hierarchical layers and that intertwined miRNA-containing FFLs are associated with party and date miRNA hubs.Using the date hub MIR858A as an example,we performed detailed molecular and genetic analyses of three interconnected miRNA-containing FFLs.These analyses revealed individual functions of the selected miRNA-containing FFLs and elucidated how the date hub miRNA fulfills multiple regulatory roles.Collectively,our findings highlight the prevalence and importance of miRNA-containing FFLs,and provide new insights into the design principles and control logics of miRNA regulatory networks governing gene expression programs in plants. | Zhaoxu Gao Jun Li Li Li Yanzhi Yang Jian Li Chunxiang Fu Danmeng Zhu Hang He Huaqing Cai Lei Li | 2022 | Genomics, Proteomics & Bioinformatics2022，20，4： | 1 |
| 7 | Effect of Bath Concentration on Coagulation Kinetics at the Early Stage during Wet Spinning of PAN Copolymer Nascent Fibers显示文摘 | Pucha Zhou Chunxiang Lu Jiyou Shi Kaixi Li Fu He Shouchun Zhang Yonghong Li | 2011 | Journal of Macromolecular Science Part B2011，，6： | 1 |
| 8 | A novel DNA microarray for rapid diagnosis of enteropathogenic bacteria in stool specimens of patients with diarrhea显示文摘 | Yuanhai You Chunxiang Fu Xun Zeng Daishan Fang Xiaomei Yan Baochun Sun Di Xiao Jianzhong Zhang | 2008 | Journal of Microbiological Methods2008，，： | 1 |
| 9 | Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height显示文摘Switchgrass(Panicum virgatum L.)is an important perennial,noninvasive,tall ornamental grass that adds color and texture to gardens and landscapes.Moreover,switchgrass has been considered a forage and bioenergy crop because of its vigorous growth,low-input requirements,and broad geography.Here,we identified PvWOX3a from switchgrass,which encodes a WUSCHEL-related homeobox transcription factor.Transgenic overexpression of PvWOX3a in switchgrass increased stem length,internode diameter,and leaf blade length and width,all of which contributed to a 95%average increase in dry weight biomass compared with control plants.Yeast one-hybrid and transient dual-luciferase assays showed that PvWOX3a can repress the expression of gibberellin 2-oxidase and cytokinin oxidase/dehydrogenase through apparently direct interaction with their promoter sequences.These results suggested that overexpression of PvWOX3a could increase gibberellin and cytokinin levels in transgenic switchgrass plants,which promotes cell division,elongation,and vascular bundle development.We also overexpressed PvWOX3a in a transgenic miR156-overexpressing switchgrass line that characteristically exhibited more tillers,thinner internodes,and narrower leaf blades.Double transgenic switchgrass plants displayed significant increases in internode length and diameter,leaf blade width,and plant height but retained a tiller number comparable to that of plants expressing miR156 alone.Ultimately,the double transgenic switchgrass plants produced 174%more dry-weight biomass and 162%more solubilized sugars on average than control plants.These findings indicated that PvWOX3a is a viable potential genetic target for engineering improved shoot architecture and biomass yield of horticulture,fodder,and biofuel crops. | Ruijuan Yang Zhenying Wu Chen Bai Zhichao Sun Mengqi Wang Yuzhu Huo Hailing Zhang Yamei Wang Huapeng Zhou Shaojun Dai Wenwen Liu Chunxiang Fu | 2021 | Horticulture Research2021，8，1： | 1 |
| 10 | Functional characterization of caffeic acid O-methyltransferase in internode lignification of switchgrass(Panicum virgatum)显示文摘Caffeic acid O-methyltransferase(COMT) is a crucial enzyme that mainly methylates phenylpropanoid meta-hydroxyl of C5 in the biosynthesis of syringyl lignin in angiosperms. A putative COMT, named as PvCOMT1,was isolated from switchgrass(Panicum virgatum), a C4 warm-season dual-purpose forage and bioenergy crop. Our results showed that recombinant PvCOMT1 enzyme protein catalyzed the methylation of 5-OH coniferyl alcohol, 5-OH coniferaldehyde(CAld5H) and 5-OH ferulic acid. Further in vitro studies indicate that CAld5H can dominate COMT-mediated reactions by inhibiting the methylation of the other substrates. Transgenic switchgrass plants generated by an RNAi approach were further employed to study the function of COMT in internode lignification. A dramatic decrease in syringyl lignin units coupled with an obvious incorporation in 5-OH guaiacyl lignin units were observed in the COMT-RNAi transgenic plants. However, the constitutive suppression of COMT in switchgrass plants altered neither the pattern of lignin deposition along the stem nor the anatomical structure of internodes. Consistent with the biochemical characterization of PvCOMT1, a significant decrease in sinapaldehyde was found in the COMT-RNAi transgenic switchgrass plants, suggesting that CAld5H could be the optimal intermediate in the biosynthesis syringyl lignin. | Fengyan WU Zhenying WU Aiguo YANG Shanshan JIANG Zeng-Yu WANG Chunxiang FU | 2018 | Frontiers of Agricultural Science and Engineering2018，5，1： | 0 |
| 11 | The role of Class Ⅱ KNOX family in controlling compound leaf patterning in Medicago truncatula显示文摘Compound leaf development requires the coordination of genetic factors, hormones, and other signals. In this study, we explored the functions of Class Ⅱ KNOTTED-like homeobox(KNOXII)genes in the model leguminous plant Medicago truncatula. Phenotypic and genetic analyses suggest that Mt KNOX4, 5 are able to repress leafet formation, while Mt KNOX3, 9, 10 are not involved in this developmental process. Further investigations have shown that Mt KNOX4 represses the CK signal transduction, which is downstream of Mt KNOXⅠ-mediated CK biosynthesis. Additionally,two boundary genes, FUSED COMPOUND LEAF1(orthologue of Arabidopsis Class M KNOX) and NO APICAL MERISTEM(orthologue of Arabidopsis CUP-SHAPED COTYLEDON), are necessary for Mt KNOX4-mediated compound leaf formation.These findings suggest, that among the members of Mt KNOXⅡ, Mt KNOX4 plays a crucial role in integrating the CK pathway and boundary regulators,providing new insights into the roles of Mt KNOXⅡ in regulating the elaboration of compound leaves in M. truncatula. | Xiao Wang Juanjuan Zhang Maofeng Chai Lu Han Xiaohua Cao Jing Zhang Yiming Kong Chunxiang Fu Zeng‐Yu Wang Kirankumar SMysore Jiangqi Wen Chuanen Zhou | 2023 | Journal of Integrative Plant Biology2023，65，10： | 0 |
| 12 | A Flaw in the Security Proof of BDOP-PEKS and PEKS-STAT显示文摘Provable security has been widely used for analyzing the security of cryptosystems. Its main idea is to reduce the secu- rity to some well-defined computational assumption. The reduc- tion process is called the security proof. In this paper, we find a flaw in the security proof of BDOP-PEKS and PEKS-STAT, pre- sent a new conclusion for the security of BDOP-PEKS, and give a security proof. The flaw in the security proof of PEKS-STAT can be fixed in the same way. Finally we conclude some steps of secu- rity proof, and emphasize that the probability is as important as the construction. | ZHANG Yajuan ZHU Yuefei GU Chunxiang FU Changchun | 2007 | Wuhan University Journal of Natural Sciences2007，12，1： | 0 |
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