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您的检索式：作者名="Jozef Samaj"
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| 1 | Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells显示文摘Arabidopsis BRASSINOSTEROID INSENSITIVE1 (BRI1 ) 的主要 brassinosteroid (BR ) 受体在 BR 发信号,而是在 BRI1 成为主观和集会状态遗体上位于 BR 的效果下面的分子的机制起基本作用不清楚。这里,我们使用了可变角度总数内部思考荧光显微镜学和荧光分析动力学的跨关联的光谱学标注 ofGFP 的 BRI1。我们发现了那,响应 BR,有 AtFlot1-mCherry 的 BRI1-GFP 的合作本地化的度增加了,并且特别 BR 刺激了膜 BRI1 成为主观的联系 microdomain 的小径。我们也在 endocytosis 有缺点的 chc2-1 异种和 AtFlot1 amiRNA 15-5 线验证了这些观察。而且, bri1-EMS-suppressor 的 phosphorylation 地位的检查 BR 应答的基因表示的第 1a 测量表明膜 microdomains 影响 BR 发信号。这些结果 suggestthat BR 支持划分 BRI1 成功能的膜 microdomains 激活 BR 发信号。 | Li Wang Hong Li Xueqin Lv Tong Chen Ruili Li Yiqun xuei Jianjun Jiang Biao Jin Frantisek Baluska Jozef Samaj Xuelu Wang Jinxing Lin | 2015 | Molecular Plant2015，8，9： | 11 |
| 2 | YODA-HSP90 Module Regulates Phosphorylation-Dependent Inactivation of SPEECHLESS to Control Stomatal Development under Acute Heat Stress in Arabidopsis显示文摘Stomatal ontogenesis,patterning,and function are hallmarks of environmental plant adaptation,especially to conditions limiting plant growth,such as elevated temperatures and reduced water availability.The specification and distribution of a stomatal cell lineage and its terminal differentiation into guard cells require a master regulatory protein phosphorylation cascade involving the YODA mitogen-activated protein kinase kinase kinase.YODA signaling results in the activation of MITOGEN-ACTIVATED PROTEIN KINASEs(MPK3 and MPK6),which regulate transcription factors,including SPEECHLESS(SPCH).Here,we report that acute heat stress affects the phosphorylation and deactivation of SPCH and modulates stomatal density.By using complem entary molecular,genetic,biochemical,and cell biology approaches,we provide solid evidence that HEAT SHOCK PROTEINS 90(HSP90s)play a crucial role in transducing heat-stress response through the YODA cascade.Genetic studies revealed that YODA and HSP90.1 are epistatic,and they likely function linearly in the same developmental pathway regulating stomata formation.HSP90s interact with YODA,affectits cellular polarization,and modulate the phosphorylation of downstream targets,such as MPK6 and SPCH,under both normal and heat-stress conditions.Thus,HSP90-mediated specification and differentiation of the stomatal cell lineage couples stomatal development to environmental cues,providing an adaptive heat stress response mechanism in plants. | Despina Samakovli Tereza Ticha Tereza Vavrdova Miroslav Ovecka Ivan Luptovciak Veronika Zapletalova Anna Kucharova Pavel Krenek Yuliya Krasylenko Theoni Margaritopoulou Loukia Roka Dimitra Milioni George Komis Polydefkis Hatzopoulos Jozef Samaj | 2020 | Molecular Plant2020，13，4： | 9 |
| 3 | Arabidopsis Profilin Isoforms,PRF1 and PRF2 Show Distinctive Binding Activities and Subcellular Distributions显示文摘Profilin is an actin-binding protein that shows complex effects on the dynamics of the actin cytoskeleton. There are five profilin isoforms in Arabidopsis thaliana L. However, it is still an open question whether these isoforms are functionally different. In the present study, two profilin isoforms from Arabidopsis, PRF1 and PRF2 were fused with green fluorescent protein (GFP) tag and expressed in Escherichia coli and A. thaliana in order to compare their biochemical properties in vitro and their cellular distributions in vivo. Biochemical analysis revealed that fusion proteins of GFP-PRF1 and GFP-PRF2 can bind to poly-L-proline and G-actin showing remarkable differences. GFP-PRF1 has much higher affinities for both poly-L-proline and G-actin compared with GFP-PRF2. Observations of living cells in stable transgenic A. thaliana lines revealed that 35S::GFP-PRF1 formed a filamentous network, while 35S::GFP-PRF2 formed polygonal meshes. Results from the treatment with latrunculin A and a subsequent recovery experiment indicated that filamentous alignment of GFP-PRF1 was likely associated with actin filaments. However, GFP-PRF2 localized to polygonal meshes resembling the endoplasmic reticulum. Our results provide evidence that Arabidopsis profilin isoforms PRF1 and PRF2 have different biochemical affinities for poly-L-proline and G-actin, and show distinctive localizations in living cells. These data suggest that PRF1 and PRF2 are functionally different isoforms. | Feng ang Yanping Jing Zhen ang Tonglin Mao Jozef Samaj Ming Yuan Haiyun Ren | 2009 | Journal of Integrative Plant Biology2009，51，2： | 1 |
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