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| 1 | Virulence and potential pathogenicity of coccoid Helicobacter pylori induced by antibiotics显示文摘AIM To explore the virulence and the potential pathogenicity of coccoid Helicobacter pylori (H. pylori) transformed from spiral form by exposure to antibiotic.METHODS Three strains of H. pylori, isolated from gastric biopsy specimens of confirmed peptic ulcer, were converted from spiral into coccoid from by exposure to metronidazole.Both spiral and coccoid form of H. pylori were tested for the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells, and the differences of the protein were analysed by SDS-PAGE and Western blot,The mutation of the genes including ureA, ureB,hpaA; vacA and cagA, related with virulence,was detected by means of PCR and PCR-SSCP.RESULTS In the coccoid H. pylori, the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells alldecreased. In strain F44, the rate and index of adherence reduced from 70.0% ± 5.3% to 33% ±5.1% and from 2.6 ±0.4 to 0.96 ±0.3 (P<0.01),respectively. The invasion of coccoid H. pylori into Hep-2 cell could be seen under electronmicroscope. SDS-PAGE showed that the content of the protein with the molecular weight over Mr74 000 decreased, and the hybriditional signal in band Mr 125 000 weakened, while the band Mr 110000 and Mr63000 strengthened in coccoid H. pylori as shown in Western blot. The results of PCR were all positive, and PCR-SSCP indicated that there may exist the point mutation in gene hpaA or vacA.CONCLUSION The virulence and the proteins with molecular weight over Mr74 000 in coccoid H. pylori decrease, but no deletion exists in amplification fragments from ureA, ureB, hpaA,vacA and cagA genes, suggesting that coccoid H. pylori may have potential pathogenicity. | Fei Fei She1 Dong Hui Su1 Jian Yin Lin2 Lin Ying Zhou3 1Department of Microbiology, Fujian Medical University. Fuzhou 350004, Fujian Province, China2Department of Molecular Medicine, Fujian Medical University, Fuzhou 350004, Fujian Province, China 3Laboratory of Electron Microscope, Fujian Medical University, Fuzhou 350004. Fujian Province. ChinaFei Fei She. graduated from Fujian Medical University as a postgraduate in 1991, now associate professor of microbiology and immunology, specialized in molecular biology of pathogen, having 15 papers published. | 2001 | World Journal of Gastroenterology2001，7，2： | 18 |
| 2 | Improvedendoscopicretrogradecholangiopancreatographybrushincreases diagnosticyieldofmalignantbiliarystrictures显示文摘AIM: To determine if a new brush design could im-prove the diagnostic yield of biliary stricture brushings. METHODS: Retrospective chart review was performed of all endoscopic retrograde cholangiopancreatography procedures with malignant biliary stricture brushing between January 2008 and October 2012. A standard wire-guided cytology brush was used prior to proto-col implementation in July 2011, after which, a new 9 French wire-guided cytology brush(Infinity sampling device, US Endoscopy, Mentor, OH) was used for all cases. All specimens were reviewed by blinded pa-thologists who determined whether the sample waspositive or negative for malignancy. Cellular yield was quantified by describing the number of cell clusters seen. RESULTS: Thirty-two new brush cases were compared to 46 historical controls. Twenty-five of 32 (78%) cases in the new brush group showed abnormal cellular find-ings consistent with malignancy as compared to 17 of 46(37%) in the historical control group(P = 0.0003). There was also a significant increase in the average number of cell clusters of all sizes(21.1 vs 9.9 clusters, P = 0.0007) in the new brush group compared to his-torical controls. CONCLUSION: The use of a new brush design for brush cytology of biliary strictures shows increased di-agnostic accuracy, likely due to improved cellular yield, as evidenced by an increase in number of cellular clus-ters obtained. | Shieh FK Luong-Player A Khara HS Liu H Lin F Shellenberger MJ Johal AS Diehl DL | 2014 | World Journal of Gastrointestinal Endoscopy2014，6，7： | 4 |
| 3 | 胆固醇酯转移蛋白基因的蛋白质截断型变异体与冠状动脉性心脏病风险的关系显示文摘随机对照试验结果表明,抑制胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)的疗法并不能降低冠状动脉性心脏病(coronary heart disease,CHD)的发生风险。研究失败的可能原因包括靶目标无效、靶目标外小分子的不良反应和随机对照设计因素影响等。在编码药物靶点的基因中具有天然存在的遗传变异,以此为基础,人类研究可以深入了解针对基因产物的治疗的潜在功效和安全性。 | Nomura A Won HH Khera AV Takeuchi F Ito K McCarthy S Emdin CA Klarin D Natarajan P Zekavat SM Gupta N Peloso GM Borecki IB Teslovich TM Asselta R Duga S Merlini PA Correa A Kessler T Wilson JG Bown MJ Hall AS Braund PS Carey DJ Murray MF Kirchner HL Leader JB Lavage DR Manus JN Hartze DN Samani NJ Schunkert H Marrugat J Elosua R McPherson R Farrall M Watkins H Juang JJ Hsiung CA Lin SY Wang JS Tada H Kawashiri MA Inazu A Yamagishi M Katsuya T Nakashima E Nakatochi M Yamamoto K Yokota M Momozawa Y Rotter JI Lander ES Rader DJ Danesh J Ardissino D Gabriel S Willer CJ Abecasis GR Saleheen D Kubo M Kato N Ida Chen YD Dewey FE Kathiresan S 刘莉 叶鹏 | 2017 | 中华高血压杂志2017，25，9： | 2 |
| 4 | Robotic surgery techniques to approach benign prostatic hyperplasia disease:A comprehensive literature review and the state of art显示文摘Objective:The robotic-assisted approach to simple prostatectomy(RASP)was conceived,essentially reproducing the fundaments of open simple prostatectomy.Since the first report,RASP underwent several technical modifications.The study aims to identify and describe the current robotic surgery techniques to approach benign prostatic hyperplasia(BPH).Methods:The paper performed a non-systematic literature review accessing PubMed and Embase databases for all full-text articles published from 2008 to May 2020,assessing robot-assisted surgical techniques for BPH treatment using the terms“robot-assisted simple prostatectomy”OR“robotic simple prostatectomy”OR“RASP”AND“surgical technique”.Results:After careful review of 180 studies in PubMed and 198 in Embase,16 papers reporting different RASP techniques.After the first procedure described by Sotelo et al.[9],several authors contributed to the development of the RASP technique.John et al.[24]proposed the extraperitoneal access,and Yuh et al.[23]first reported the adenoma transcapsular dissection.Some modifications were proposed by Coelho et al.[31]on trigonization,posterior reconstruction,and urethro-vesical anastomosis.Other groups focused on urethral-preserving procedures.Moschovas et al.[28]and Clavijo et al.[32]recently described an intrafascial RASP with the removal of the entire prostatic tissue.Finally,Kaouk et al.[29]reported the feasibility and safety of the da Vinci Single Port approach.Conclusion:In the last eighteen years,the robotic-assisted approach to BPH disease has been evolved,and different techniques have been described.This review details all the technical developments on RASP that distinctive groups have proposed since the multiport robotic platforms until the new da Vinci Single Port. | Marcio Covas Moschovas Frederico Timo´teo Leonardo Lins Ose´as de Castro Neves Kulthe Ramesh Seetharam Bhat Vipul R.Patel | 2021 | Asian Journal of Urology2021，8，1： | 2 |
| 5 | Prenatal detection of embryo resorption in osteopontin-deficient mice using serial noninvasive magnetic resonance microscopy显示文摘 | Weintraub AS Lin X Itskovich VV | 2004 | Pediatric Research2004，55，： | 1 |
| 6 | Prenatal detection of embryo resorption in osteopontin - deficient mice using serial noninvasive magnetic resonance microscopy 显示文摘 | Weintraub AS Lin X hskovich VV | 2004 | Pediatr Res2004，55，： | 1 |
| 7 | Gene-based partial least-squares approaches for detecting rare variant associations with complex traits 显示文摘 | Turkmen AS Lin S | 2011 | BMC Proc2011，59，： | 1 |
| 8 | Separation of phospholipids in microfluidic chip device: application to high- throughput screening assays for lipid - modifying enzymes 显示文摘 | Lin S Fischl AS Bi X | 2003 | Anal Biochem2003，314，1： | 1 |
| 9 | Multiple pathways of repair of DNA double-strand breaks in mammalian chromosomes显示文摘 | Lin Y Lukacsovich T Waldman AS | 1999 | Mol Cell Biol1999，19，： | 1 |
| 10 | Monoclonal antibody against E selectin attenuates subarach- noid hemorrhage-induced cerebral vasospasm显示文摘 | Lin CL Dumont AS Calisaneller T Kwan AL Hwong SL Lee KS | 2005 | Surg Neurol2005，64，3： | 1 |
| 11 | Phase Ⅱ study of flavopiridol in relapsed chronic lymphocytic leukemia demonstrating high response rates in genetically high-risk disease 显示文摘 | Lin TS Ruppert AS Johnson AJ | 2009 | J Clin Oncol2009，27，35： | 1 |
| 12 | Localized 3D analysis of cartilage composition and morphology in small animal models of joint degeneration 显示文摘 | Thote T Lin AS Raji Y | 2013 | Osteoarthritis Cartilage2013，21，8： | 1 |
| 13 | Localized 3D analysis of cartilage composition and morphology in small animal models of joint degener- ation显示文摘 | Thote T Lin AS Raji Y | 2013 | Osteoarthr Cartilage2013，21，8： | 1 |
| 14 | Ultrasonic communicationin frogs显示文摘 | Feng AS PM Narins CH Xu WY Lin ZL Yu Q Qiu ZM Xu and JX Shen | 2006 | Nature2006，440，： | 1 |
| 15 | Inhibition of lymphogenous metastasis using adeno-associated virus-mediated gene transfer of a soluble VEGFR-3 decoy receptor显示文摘 | Lin J Lalani AS Harding TC | 2005 | Cancer Res2005，65，15： | 1 |
| 16 | Quantification of cerebral glucose metabolic rate in mice using 18F-FDG and small-animal PET显示文摘 | Yu AS Lin HD Huang SC | 2009 | J Nucl Med2009，50，6： | 1 |
| 17 | Autophagy in idiopathic pulmonary fibrosis显示文摘 | Patel AS Lin L Geyer A | 2012 | PLoS One2012，7，41： | 1 |
| 18 | Bronchoscopic electrocautery for palliation of post-anastomotic tracheal stricture in a patient with complete tracheal transection following blunt chest trauma显示文摘 | Chung YH Lu HI Lin AS | 2005 | Chang Gung Med J2005，28，10： | 1 |
| 19 | Cardiac efficiency is improved after ischemia by altering both the source and fate of protons显示文摘 | Lin B Clanachan AS Schulz R | 1996 | Circ Res1996，79，： | 1 |
| 20 | Characterization of perioperative seizures and epilepsy following aneurismal sub- arachnoid hemorrhage 显示文摘 | Lin CL Dumont AS Lieu AS | 2003 | Journal of Neurosurgery2003，99，： | 1 |
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