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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | The prognostic value of preoperative serum levels of CEA,CA19-9 and CA72-4 in patients with colorectal cancer显示文摘INTRODUCTIONCarcinoembryonic antigen ( CEA) , originally described by Gold and Freedman [1] in 1965, is now an acknowledged member of immunoglobulin superfamily[2],with a role as an intracellular adhesion molecule[3].Carbohydrate antigen 19-9(CA19-9), obtained with a monoclonal antibody produced by immunizing a monoclonal antibody produced by immunizing a mouse with a colonic cancer cell line in 1979[4],is a ligand for E-selectin that plays an important role in the addhesion of cancer cells to endothelial cells [5,6]. | Chao Xu Zheng~1 Wen Hua Zhan~1 Ji Zong Zhao~2 Dong Zheng~3 Dong Ping Wang~1 Yu Long He~1 Zhang Qing Zheng~1 ~1Department of General Surgery,~2Laboratory of Surgery,~3Department of Medicine,First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,Guangzhou 510080,Guangdong Province,ChinaDr.Chao Xu Zheng now working as a surgeon and lecturer in the Department of General Surgery,First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,who is a Ph.D.student,having 7 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 25 |
| 3 | Gastrin,somatostatin,and experimental disturbance of the gastrointestinal tract in rats显示文摘INTRODUCTIONThe field of gastrointestinal hormones has expanded at a dizzying rate[1-4].Gastrointestinal hormones as regulatory peptides that appear to be major components of bodily integration and have important regulatory actions on physioligical function of the gastrointestinal tract .The successful isolation of some gastrointestinal hormones and the development of sensitive methods for their detection have led to the unexpected finding that they also exist in the brain . | Yong Li Yao~1 Bo Xu~2 Wan Dai Zhang~1 Yu Gang Song~1 ~1Institute of Gastrointestinal Diseases,Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2Department of Orthopedics Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaYong Li Yao graduated from First Military Mcdical University with a master degree in 1998.She now works in the Institute of Gastrointestinal Diseases,Nanfang Hospital as a doctoral candidate majoring gastrointestinal diseases and gastrointestinal hormones,she has published 8 papers. | 2001 | World Journal of Gastroenterology2001，7，3： | 12 |
| 4 | Nat Genet:单基因突变导致过敏性皮炎的发生显示文摘最近,研究者们鉴定出了一类导致神经性皮炎发生的关键基因突变:CARDll。来自美国NIH过敏与传染病研究所的研究者们通过对四个没有血缘关系的患病家庭进行分析,发现了这一导致疾病产生的基因、 | Chi A Ma, Yuan Zhang, Michael A Weinreich, Jonathan J Lyons, Celeste G Nelson, Thomas DiMaggio, Kelly D Stone, Joshua D Milner Jeffrey R Stinson, Elisa Ruffo, Batsukh Dorjbal, Swadhinya Arjunaraja, Kelsey Voss, Andrew L Snow Jordan K Abbott, Pia J Hauk, Paul R Reynolds, Erwin W Gelfand Elisa Ruffo Salomé Glauzy, Natsuko Yamakawa, Eric Meffre Jennifer Stoddard, Julie Niemela, Sergio D Rosenzweig Yu Zhang, Helen F Matthews Joshua J McElwee Nina Jones Alejandro Palma, Matías Oleastro, Emma Prieto, Andrea R Bernasconi, Geronimo Dubra, Silvia Danielian, Jonathan Zaiat, Marcelo A Marti Brian Kim Megan A Cooper Neil Romberg | 2017 | 现代生物医学进展2017，17，27： | 3 |
| 5 | Hepatitis B vaccines显示文摘 | Yu AS Cheung RC Keeffe EB | 2004 | Clin Liver Dis2004，8，2： | 1 |
| 6 | Hyperfibrinolytic activity in hospitalized cirrhotic patients in a referral liver unit 显示文摘 | Hu KQ Yu AS Tiyyagura L | 2001 | Am J Gastroenterol2001，96，5： | 1 |
| 7 | Management of ascites显示文摘 | Yu AS Hu KQ | 2001 | Clin Liver Dis2001，5，2： | 1 |
| 8 | Response to tenofovir monotherapy in chronic hepatitis B patients with prior suboptimal response to entecavir显示文摘 | Pan CQ Hu KQ Yu AS | 2012 | J Viral Hepat2012，19，3： | 1 |
| 9 | Effect of acetaldehyde upon factor X and factor X a,the proteins at the gateway to the common coagulation pathway显示文摘 | Brecher AS Guyong YU Robert J | 1996 | Alcohol1996，13，： | 1 |
| 10 | Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells 显示文摘 | Yu AS McCarthy KM Francis SA | 2005 | Am J Physiol Cell Physiol2005，288，： | 1 |
| 11 | High serum interlenkin-6 level predicts future hepatocellular carcinoma development in patients with chronic hepatitis B显示文摘 | Wong VW Yu J Cheng AS | 2009 | Int J Cancer2009，124，12： | 1 |
| 12 | Total daily physical ac- tivity and the risk of AD and cognitive decline in older adults 显示文摘 | Buchman AS Boyle PA Yu L | 2012 | Neurology2012，78，17： | 1 |
| 13 | High serum interleukin-6 level predicts future hepatocellular carcinoma development in patients with chronic hepatitis B显示文摘 | Wong VW Yu J Cheng AS | 2009 | Int J Cancer2009，124，12： | 1 |
| 14 | Ultrasonic communicationin frogs显示文摘 | Feng AS PM Narins CH Xu WY Lin ZL Yu Q Qiu ZM Xu and JX Shen | 2006 | Nature2006，440，： | 1 |
| 15 | Elevated tumor necrosis factor-alpha in skeletal muscle after stroke显示文摘 | Hafer-Macko CE Yu S Ryan AS | 2005 | Stroke2005，36，9： | 1 |
| 16 | Quantification of cerebral glucose metabolic rate in mice using 18F-FDG and small-animal PET显示文摘 | Yu AS Lin HD Huang SC | 2009 | J Nucl Med2009，50，6： | 1 |
| 17 | Knockdown of occluding ex- pression leads to diverse phenotypic alterations in epithelial ceils 显示文摘 | Yu AS McCarthy KM Francis SA | 2005 | Am J Physiol Cell Physiol2005，288，6： | 1 |
| 18 | Blunt bowel and mesenteric injury: MDCT diagnosis显示文摘 | Yu J Fulcher AS Turner MA | 2011 | Abdom Imaging2011，，36： | 1 |
| 19 | NK- cell activation by LIGHT triggers tumor- specific CD8+T cell immunity to reject established tumor 显示文摘 | Fan AS Yu P Wang Y | 2006 | Blood2006，17，4： | 1 |
| 20 | Local-regional control according to surrogate markers of breast cancer subtypes and response to neoad-juvant chemotherapy in breast cancer patients undergoing breast con-serving therapy 显示文摘 | Caudle AS Yu TK Tucker SL | 2012 | Breast Cancer Res2012，14，3： | 1 |
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