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| 1 | Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification显示文摘AIM:To study whether selected bacterial 16S ribosomal RNA(rRNA)gene phylotypes are capable of disting- uishing irritable bowel syndrome(IBS). METHODS:The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant (IBS-D),eight constipation-predominant(IBS-C)and four mixed symptom-subtype(IBS-M)IBS patients,and fifteen control subjects,were analysed at three time-points with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis(PCA)with linear mixed-effects models applied to the principal component scores. RESULTS:Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component(PC1),explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups(IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05).Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy- lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects(-4.08±0.90 vs -3.33±1.16,P=0.04)and IBS-D and IBS-M subjects (-4.08±0.90 vs-3.08±1.38,P=0.05).Furthermore,a phylotype with 94%similarity to R.torques was more prevalent in IBS-D patients'intestinal micro- biota than in that of control subjects(-2.43±1.49 vs -4.02±1.63,P=0.01).A phylotype with 93%simi- larity to R.torques was associated with control sam- ples when compared with IBS-M(-2.41±0.53 vs -2.92±0.56,P=0.00).Additionally,a R.bromii-like phylotype was associated with IBS-C patients in com- parison to control subjects(-1.61±1.83 vs-3.69± 2.42,P=0.01).All of the above mentioned phylotype specific alterations were independent of the effect of time. CONCLUSION:Significant phylotype level alterationsin the intestinal microbiotas of IBS patients were observed,further emphasizing the possible contribution of the gastrointestinal microbiota in IBS. | Anna Lyra Teemu Rinttil Janne Nikkil Lotta Krogius-Kurikka Kajsa Kajander Erja Malinen Jaana Mtt Laura Mkel Airi Palva | 2009 | World Journal of Gastroenterology2009，15，47： | 26 |
| 2 | 胆汁纳米细菌的培养与鉴定显示文摘目的 探讨纳米细菌在胆汁中的分布情况及微生物学特性 ,并对其鉴定方法进行评价。方法 对 75例胆囊胆汁标本进行纳米细菌培养 ,观察其生长情况 ,并采用免疫组化染色、透射电镜扫描和钙染色进行纳米细菌鉴定。结果 胆汁中纳米细菌感染率为 6 1 3% ,其中出现完全或部分黏附于管底壁的白色沉淀者 ,纳米细菌阳性率为 75 7% ,而沉淀完全呈絮状或无沉淀者 ,阳性率为4 7 4 % ,两者间存在显著性差异。免疫组化染色阳性的胆汁标本经透射电镜扫描均发现纳米细菌。钙染色的阳性率为 38 7% ,与免疫组化染色相比较 ,其灵敏度为 5 8 7% ,特异度为 93 1% ,假阳性率6 9% ,假阴性率 4 1 3%。结论 纳米细菌单克隆抗体联合透射电镜扫描可鉴定纳米细菌 ;钙染色对纳米细菌的鉴定有着重要的参考价值 ;紧密黏附于管底的白色沉淀是纳米细菌培养过程中的一个重要微生物学特性。 | 李永国 文宇 杨竹林 魏红 刘威 谭爱玲 吴晓瑛 王群伟 黄生福 Kajander EO Ciftcioglu N | 2002 | 中华医学杂志2002，82，22： | 17 |
| 3 | Global and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome显示文摘 | Mirjana Rajili?–Stojanovi? Elena Biagi Hans G.H.J. Heilig Kajsa Kajander Riina A. Kekkonen Sebastian Tims Willem M. de Vos | 2011 | Gastroenterology2011，，5： | 8 |
| 4 | 胆囊结石患者血清、胆汁与胆囊黏膜中纳米细菌的分布研究显示文摘目的 探讨纳米细菌在正常人群血清及胆囊结石患者血清、胆汁和胆囊黏膜中的分布情况。 方法 ( 1)采用ELASA方法测定 338例正常人群和 76例胆囊结石患者血清中纳米细菌的感染情况 ;( 2 )对 5 7例胆囊结石和 18例非胆囊结石患者的胆囊胆汁进行纳米细菌培养 ,并采用免疫组化染色和透射电镜扫描进行鉴定 ;( 3)随机选取其中 40例胆囊结石患者胆囊黏膜进行免疫组化染色 ,观察相应胆汁和胆囊黏膜中纳米细菌分布的差异。 结果 ( 1)正常人群和胆囊结石患者血清中纳米细菌感染率分别为 8 0 %和 31 6 % ,两者间差异有显著性 ( χ2 =2 9 90 ,P <0 0 5 ) ;( 2 ) 75例胆汁中有46例感染纳米细菌 ,阳性率为 6 1 3 % ,其中胆囊结石患者的胆汁纳米细菌的感染率为 6 1 4 % ,非胆囊结石患者的胆汁纳米细菌的感染率为 6 1 1% ,两者间并无统计学差异 ( χ2 =0 89,P >0 0 5 )。 ( 3) 14例胆囊结石患者胆囊黏膜感染纳米细菌 ,阳性率为 35 0 % ,其主要分布在黏膜或黏膜下层及钙化灶。 结论 正常人群血清中纳米细菌感染率为 8 0 % ,胆囊结石患者血清、胆汁和胆囊黏膜中存在着纳米细菌感染 ;胆囊壁的钙化或纤维化可能与纳米细菌感染有关。 | 文宇 李永国 杨竹林 王学军 魏红 刘威 谭爱玲 苗雄鹰 王群伟 黄生福 E.Olavi Kajander Neva Ciftcioglu | 2003 | 中华外科杂志2003，41，4： | 8 |
| 5 | 慢性肝病、原发性肝癌病人血清、肝组织纳米细菌的检测显示文摘目的研究慢性肝病和原发性肝癌病人血清、组织中纳米细菌(NB)感染,为其发生机制提供新的认识。方法 55例慢性肝病(25例慢性乙肝和30例肝炎后肝硬化)、43例肝癌病人和336例健康人血清采用 ELISA、免疫组化和钙染色;53例慢性肝病(28例慢性乙肝和25例肝硬化)、43例肝癌和15例对照肝组织行免疫组化染色,部分阳性组织透射电镜观察。结果(1)慢性肝病、肝癌病人和健康人血清 ELISA 检测阳性率分别为20.0%、9.3%和8.0%,慢性肝病 NB 感染率高于健康人(P<0.05)。免疫组化染色感染率分别为14.5%、4.7%和5.7%(P<0.05)。钙染色分别为7.3%、4.7%和6.5%(P>0.05)。(2)慢性肝病、癌及癌旁、对照组免疫组化阳性率分别为:20.8%、16.3%、14.0%和0%(P>0.05)。部分肝癌和癌旁组织透射电镜观察发现纳米细菌样微生物结构(3/5)。结论部分慢性肝病、肝癌病人血清、组织中存在纳米细菌感染,慢性肝病血清中纳米细菌感染率高于健康人。 | 王学军 杨竹林 文宇 魏红 刘威 李永国 E.Olavi Kajander | 2006 | 中华肝胆外科杂志2006，12，3： | 7 |
| 6 | Elevated pro-inflammatory and lipotoxic mucosal lipids characterise irritable bowel syndrome显示文摘AIM:To investigate the pathophysiology of irritable bowel syndrome(IBS)by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.METHODS:Fifteen IBS patients fulfilling the Rome Ⅱcriteria,and nine healthy volunteers were included in the study.A combined lipidomics(UPLC/MS)and metabolomics(GC×GC-TOF)approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.RESULTS:Overall,lipid levels were elevated in patients with IBS.The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines.Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides,glycosphingolipids,and di-and triacylglycerols.Among the metabolites,the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects(P=0.03).CONCLUSION:IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile.Especially,there was an increase in several lipid species such as lysophospholipids and ceramides. | Kajsa Kajander Eveliina Myllyluoma Sinikka Kyrnpalo Martin Rasmussen Pentti Sipponen Ismo Mattila Tuulikki Seppnen-Laakso Heikki Vapaatalo Matej Orei Riitta Korpela | 2009 | World Journal of Gastroenterology2009，15，48： | 5 |
| 7 | 原发性肝癌患者血清、肝组织纳米细菌的检测显示文摘 | 王学军 杨竹林 魏红 刘威 文宇 李永国 E.Olavi Kajander | 2004 | 中华外科杂志2004，42，17： | 2 |
| 8 | Clinical Studies on Alleviating the Symptoms of Irritable Bowel Syndrorne显示文摘 | Kajander K Korpela R | 2006 | Asia Pac J Clin Nutr2006，15，4： | 1 |
| 9 | Nanobacteria-propagating calcifying nanoparticles 显示文摘 | KAJANDER E O | 2006 | Lett Appl Microbiol2006，42，6： | 1 |
| 10 | Infections,Inflamma- tion, and the risk of coronary heart disease 显示文摘 | Roivainen M Kiik M Kajander MV | 2000 | Circula - tion2000，101，3： | 1 |
| 11 | Composition and temporal stability of gastrointestinal microbiota in irritable bowel syndrome-a longitudinal study in IBS and control subjects显示文摘 | MATTO J MAUNUKSELA L KAJANDER K | 2005 | FEMS Immunol Med Microbiol2005，43，21： | 1 |
| 12 | Climate and Water: From Climate Models to Water Resources Management and Vice Versa显示文摘 | Olli Varis Tommi Kajander Risto Lemmel? | 2004 | Climatic Change2004，，3： | 1 |
| 13 | Inflammation,and the risk of coronary heart disease显示文摘 | Roivainen M Kiik M Kajander MV | | 0，，04： | 1 |
| 14 | Characteristics of nanobacteria and their possible role in stone formation显示文摘 | E. Olavi Kajander Neva Ciftcioglu Katja Aho Enrique Garcia-Cuerpo | 2003 | Urological Research2003，，2： | 1 |
| 15 | Nanobacteria: controversial pathogens in nephrolithiasis and polycystic kidney disease显示文摘 | E. Olavi Kajander Neva Ciftcioglu Marcia A. Miller-Hjelle J. Thomas Hjelle | 2001 | Current Opinion in Nephrology and Hypertension2001，，3： | 1 |
| 16 | Nanobacteria: An alternative mechanism for pathogenic intra-and extracellular calcification and stone formation Proc显示文摘 | E Olavi Kajander Neva Ciftc Ioglu | 1998 | Natl Acad Sci USA1998，95，： | 1 |
| 17 | Interaction of nanobacteria with cultured mammalian cells显示文摘 | Neva ?ift?ioglu E.Olavi Kajander | 1998 | Pathophysiology1998，，4： | 1 |
| 18 | Culprit lesion thrombus burden after manual thrombectomy or peroutaneous coronary intervention-alone in ST-segment elevation myocardial infarction : the optical coherence tomography sub-study of the TOTAL (ThrOmbecTomy versus PCI ALone) trial显示文摘 | Bhindi R Kajander OA Jolly SS | 2015 | Eur Heart J2015，36，29： | 1 |
| 19 | Spontanneous discharge originates in the dorsal root ganglion at the onset of a painful peripheral neuropathy in the rat显示文摘 | Kajander KC Wakisaka S Rennett GJ | 1992 | Neurosic Lett1992，138，： | 1 |
| 20 | Nanobacteria: an alternative mechanism for pathogenic intra- and extracellular calcifica- tion and stone formation-the journal of urology 显示文摘 | Kajander EO Ciftcioglu N | 1998 | Proc Nati Acad Sci USA1998，95，14： | 1 |
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