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| 1 | Diverse modes of clonal evolution in HBV-related hepatocellular carcinoma revealed by single-cell genome sequencing显示文摘Hepatocellular 癌(HCC ) 是癌症实质词法,基因并且 phenotypic 差异。然而,我们不理解在 intratumor 异质和肿瘤的联系词法 / 组织学的特征之间的关系。用介绍 96 个肿瘤房间(30-36 各个) 和 15 个正常的肝房间(5 各个) 的单个房间的整个染色体的定序,与联系 HBV 的 HCC 从三个男病人收集了,我们证实拷贝数字变化在 hepatocarcinogenesis 早发生,但是此后在整个肿瘤仍然保持相对稳定前进。重要地,我们证明特定的 HCC 能具有 monoclonal 或 polyclonal 起源。有汇合的 multinodular 形态学的肿瘤是典型 polyclonal 肿瘤并且显示最高的 intratumor 异质。除了 mutational 和拷贝数字侧面,我们把了用导出 HBV 的外国 genomic 标记的 HCC 的同种细胞的起源。在 monoclonal HCC,所有肿瘤单身者房间展出一样的 HBV 集成,显示 HBV 集成是一个早司机事件并且在肿瘤前进期间仍然保持极其稳定。另外,我们的结果显示那两个都转移,迟了的传播并且早播种当模特儿,在 HCC 有一个角色前进。尤其是,开始的克隆传播的早 intrahepatic 导致同步 multifocal 肿瘤的形成。同时,我们在 HCC 识别了潜在的司机基因 ZNF717,它展出变化的高频率在单个房间并且人口铺平,,通过调整 IL-6/STAT3 小径行动的肿瘤 suppressor。这些调查结果热点多重不同肿瘤在 HCC 的进化机制,它为特定的处理策略建议需要。 | Meng Duan, Shu Zhang, Zhichao Wang, Jieyi Shi, Longzi Liu, Xiaoying Wang, Aiwu Ke, Jian Zhou, Jia Fan, Qiang Gao Junfeng Hao, Chong Li Sijia Cui Daniel L Worthley Ya Cao Ruibin Xi Xiaoming Zhang Jian Zhou, Jia Fan Qiang Gao | 2018 | Cell Research2018，28，3： | 21 |
| 2 | Bottom water temperature measurements in the South China Sea,eastern Indian Ocean and western Pacific Ocean显示文摘文章报道了一批新的海底底水温度(BWT)数据,其中南海(SCS)158个站位、东印度洋(EIO)30个站位及西太平洋(WPO)37个站位。基于这批新的BWT数据,获得南海和西太平洋海域底水温度与水深经验关系,可为地球物理和物理海洋提供准确、可靠的海底温度边界。这将有助于海底油气资源调查与评估。同时,这批实测数据表明:1)水深超过3500m的海域,其底水温度在南海约为2.47℃,比东印度洋(~1.34℃)和西太平洋(~1.60℃)稍微偏高。这与大洋传送带模式所预测的情况比较吻合。该模式认为:低温高盐的海水,从北大西洋格陵兰岛和冰岛附近海域下沉到深层,然后向南流动,再与南极洲周围海域的低温高盐海水一同向北进入印度洋和太平洋。而南海是一个相对比较封闭的热带边缘海,其内部海水与印度洋和菲律宾海交换有限,导致海水温度整体高于印度洋和太平洋。2)台西南盆地水深在2700~3000m的部分站位,其底水温高达约3.00℃,明显高于其周边同水深海域底水温度(平均值约为2.33℃)。这可能是台西南盆地海底水热活动导致的结果。3)在东印度洋和西太平洋水深超过4800m海域,底水温度随着水压增大稍有升高,其升高率分别为10.6mK·MPa^(-1)和12.0mK·MPa^(-1)。这与理论估算的深层底水绝热压力温度梯度范围较为吻合。这也意味着东印度洋和西太平洋深层底水,主要由绝热自压作用导致其温度随着深度的增大而升高。 | YANG Xiaoqiu SHI Xiaobin ZHAO Junfeng YU Chuanhai GAO Hongfang CHEN Aihua LU Yuanzheng CEN Xianrong LIN Weiren ZENG Xin XU Hehua REN Ziqiang ZHOU Shengqi XU Ziying SUN Jinlong KAMIYA Nana LIN Jian | 2018 | 热带海洋学报2018，37，5： | 8 |
| 3 | Synergistic graphene/aluminum surface plasmon coupling for zinc oxide lasing improvement显示文摘免费电子的集体摆动在材料的表面上产生电浆子。耳语画廊 microcavity 有效地从它的内部墙基于全部的思考在它的表面上限制轻地。当这些二种电磁波遇见对方时,多于在耳语画廊 microcavity 与 graphene 和艾尔 nanoparticles 的单层是涂的以后, 50 褶层和阀值被减少,从单个 ZnO microrod 的刺激排出物被提高。放射激光的表演的改进被归因于与 ZnO 激子 graphene/Al 表面电浆子联合的 synergistic 精力。放射激光的特征和联合机制系统地被调查。 | Qiuxiang Zhu Feifei Qin Junfeng Lu Zhu Zhu Haiyan Nan Zengliang Shi Zhenhua Ni Chunxiang Xu | 2017 | Nano Research2017，10，6： | 6 |
| 4 | FREQUENCY OVERLAPPED SIGNAL IDENTIFICATION USING BLIND SOURCE SEPARATION显示文摘主要部件分析(PCA ) 和独立部件分析(集成通信适配器) 的概念,原则和用法被解释。然后算法和方法论基于 ofICA 的瞎的来源分离(BSS ) ,在哪个基于 PCA 预先增白因为观察信号被使用,被研究。瞄准混合发信号,部件其频率被对方重叠, BSS 的模拟由使用 BSS 的理论和途径发信号分开这类混合被做了。结果证明 BSS 有频率分析的传统的方法论没有的一些优点。 | WANG Junfeng SHI Tielin HE Lingsong YANG Shuzi | 2006 | Chinese Journal of Mechanical Engineering2006，19，2： | 6 |
| 5 | Clinical effects of Xingnao Kaiqiao acupuncture on neurological impairment following cerebral infarction显示文摘BACKGROUND: Although the curative effects of acupuncture have been confirmed by various treatments of cerebral infarction, few studies have investigated when acupuncture can attain the best clinical effect. OBJECTIVE: Four different time points were selected for acupuncture treatment of cerebral infarction to evaluate the appropriate time course for Xingnao Kaiqiao therapy in terms of improved neurological function. DESIGN: Controlled observation. SETTING: Department of Traditional Chinese Medicine Rehabilitation and Physiotherapy of the Affiliated Hospital of Medical College of Chinese Armed Police Forces. PARTICIPANTS: A total of 120 inpatients with cerebral infarction of different stages, including 75 males and 45 females, aged 41-75 years, were selected from November 2005 to December 2006 at the Department of Traditional Chinese Medicine Rehabilitation and Physiotherapy in Affiliated Hospital of Medical College of Chinese Armed Police Forces. Diagnostic criteria: in accordance with 'main points of diagnosis on different cerebrovascular disease' secondly revised in the Second Cerebrovascular Disease Academic Meeting of Chinese Medicine Association in 1986. All accepted subjects provided confirmed consent, and the experiment received ethical permission from the hospital’s ethics committee. METHODS: ① Experiment grouping: All inpatients were divided into four groups with non-stochastic concurrent control method according to the disease course: Group I (onset within 7 hours), group Ⅱ (onset from 7 hours to 3 days), group Ⅲ (onset within 4-7 days), and group Ⅳ (onset within 21-180 days). On the basis of symptomatic treatment with western medicine, each group received Xingnao Kaiqiao therapy after onset within 7 hours, 7 hours to 3 days, 4 to 7days, and 21 to 180 days. ② The principal acupoints were Neiguan, Renzhong, and Sanyinjiao. ③ The auxiliary acupoints were Jiquan, Chize, and Weizhong. ④Acupuncture manipulations: initially, Neiguan (PC6, bilateral) was needled at 0.5-1.0 cun vertically with a reducing technique achieved by rotating-swirling and lifting-inserting for 1 minute. Subsequently, Renzhong (GV26) was needled at 0.3-0.5 cun obliquely towards the nasal septum, with a heavy bird-pecking method until eyeballs were moist or shed tears. Sanyinjiao (SP6, affected limb) was needled at 1.0-1.5 cun obliquely along the medial border of the tibia with a 45° angle between the needle and skin. The technique of reinforcing was achieved by a lifting-inserting method until the affected lower limb twitched three times. The auxiliary acupoint, Jiquan, was selected when the patient extended the affected upper limb abduction. Jiquan was along the downward channel, 1 cun from the quondam Jiquan (HT1) acupoint; the armpit hair was avoided. Jiquan (affected limb) was needled vertically at 1.0-1.5 cun. The technique of reduction was achieved by lifting-inserting until the affected upper limb twitched three times. Chize (LU5) was selected when the patient flexed the elbow at 120°. Chize was vertically needled at 1 cun with a lifting-inserting reducing technique until the affected forearm and fingers twitched three times. Weizhong (BL40) was selected when the patient’s leg was raised straight in a supine position. Weizhong was vertically needled at 0.5-1.0 cun with a lifting-inserting reducing technique until the affected lower limb twitched three times. ⑤ Acupuncture manipulations were achieved by the doctor who is an associate chief physician and doctor of acupuncture and moxibustion and has aptitude for the post’s specification. ⑥One course of treatment lasted for 10 days and each group was treated for two courses of treatment. ⑦Experiment evaluation: To apply neurological impairment scores for evaluating clinical curative effects before and after treatment with Xingnao Kaiqiao therapy on day 7, 14, and 21(range of 0-45; decreasing score indicates functional improvement).To evaluate clinical curative effect by clinical effective power:Basic recovery: equal to ormore than 81%; significant improvement: 56% to 81% (including 56%); Improvement: between 36% to 56% (including 36%); Slight Improvement: 11% to 36% (including 11%); No change: less than 11%; Aggravation (including death): negative value. Total effective rate was computed at the same time. MAIN OUTCOME MEASURES: The total effective rate and amelioration of neurological impairment of the patients after treatment. RESULTS: All 120 patients with cerebral infarction were included in the final analysis, without deletion. ① Comparison of the total effective rate: the total effective rate of group Ⅰ, group Ⅱ, group Ⅲ, and group Ⅳ was 97%, 93%, 93%, and 67%, respectively. There were significant differences of interclass synthetic curative effects (χ2 = 36.351 7, P < 0.01). ② Amelioration of neurological impairment: A decreased neurological impairment score was observed in group I on day 7, 14, and 21 after Xingnao Kaiqiao therapy. The differences between group IV and the remaining three groups were significant (P < 0.05). CONCLUSION: Xingnao Kaiqiao therapy is helpful for amelioration of neurological impairment in patients during the acute stage cerebral infarction. | Jie Xiong Lina Ning Jinling Bian Jun Li Junfeng Xu Zhilong Zhang Jiakui Guo YadongLi Xuemin Shi | 2008 | Neural Regeneration Research2008，3，3： | 5 |
| 6 | Plasmon enhancement for Vernier coupled single-mode lasing from ZnO/Pt hybrid microcavities显示文摘开发单个模式操作并且改进放射激光以便保证 microlasers 和 nanolasers 的实际适用性的表演是必要的。在这份报纸,有 ZnO microcomb 的改变的 nanoscaled 空气差距的二六角形的 microteeth 被用来构造联合耳语画廊洞。这被做完成没有要求任何复杂或复杂的操作完成与 nanoscale 精确放,基于游标效果放射激光的一个稳定的单个模式。光获得和相应紫外放射激光表演通过结合磅 nanoparticles 的局部性的表面电浆子极大地被改进。ZnO/Pt 混合 microcavities 完成了单个模式与更高的方面模式抑制比率和更低的阀值放射激光的紧张的七倍的改进。导致了这改进的机制详细被描述了。 | Yueyue Wang Feifei Qin Junfeng Lu Jitao Li Zhu Zhu Qiuxiang Zhu Ye Zhu Zengliang Shi Chunxiang Xu | 2017 | Nano Research2017，10，10： | 5 |
| 7 | Plasmon-enhanced ZnO whispering-gallery mode lasing显示文摘 | Chunxiang Xu Feifei Qin Qiuxiang Zhu Junfeng Lu Yueyue Wang Jitao Li Yi Lin Qiannan Cui Zengliang Shi Arumugam Gowri Manohari | 2018 | Nano Research2018，11，6： | 4 |
| 8 | Effects of extracts from Chuanwu(Aconitum Carmichaelii) and Banxia(Rhizoma Pinelliae) on excisional wound healing in a rat's model显示文摘OBJEVTIVE: To investigate the effects of extracts from Chuanwu(Aconitum Carmichaelii) and Banxia(Rhizoma Pinelliae) on the excisional wound healing in a rat's model.METHODS: Rats were performed a surgical lesion with a 2.0 cm resected tissue in the dorsal fascia.Following, animals were randomly divided into model group, YNB group and APE group those were respectively treated with saline, 1 mg/mL of Yunnan Baiyao and 1 mg/mL of Chuanwu(Aconitum Carmichaelii) and Banxia(Rhizoma Pinelliae) extracts. Wound contractions in days 0, 3, 7, 14 and 21 were calculated by an image analyzer. Histological analysis was analyzed using hematoxilin and eosin.Levels of tumor necrosis factor-2(IL-2), IL-4, IL-10, transformαin(TNF-g growα), interleukinth factor-1(TGF-nd basic fibroblast growth factor(bFββ1) a-GF) were determined by real-time quantitative PCR.RESULTS: Compared with that of YNB group and APE gtoup, the skin of rats showed poor re-modeling and re-epithelization characterized by a significant decrease of neovascularization, epithelialization and fibroblast in the model group. In the APE group, levels of TNF-α-and IL-2 were significantly down-regulated and IL4 and IL-10 significantly upregulated in contrasted with that of model group.In addition, levels of TGF-roup were significantly iβ1 and bFGF in the APE gnduced compared with that of model group.CONCLUSION: The results suggest that the extracts from Chuanwu(Aconitum Carmichaelii) and Banxia(Rhizoma Pinelliae) promote wound healing in the rats, which is associated with enhancing antiinflammatory ability and inducing fibroblast formation. | Xia Xichao Ma Yuhong Wang Fuan Zheng Xinhua Liu Yang Zhang Junfeng Cui Juan Shi Bingqin Li Hongwen Liu Rongzhi Zhang Yaping Cheng Zhaofei Han Xiaolong | 2019 | Journal of Traditional Chinese Medicine2019，39，1： | 4 |
| 9 | Application of a neural network system combined with genetic algorithm to rank coalbed methane reservoirs in the order of exploitation priority显示文摘A new method based on the combination of a neural network and a genetic algorithm was proposed to rank the order of exploitation priority of coalbed methane reservoirs.The neural network was used to acquire the weights of reservoir parameters through sample training and genetic algorithm was used to optimize the initial connection weights of nerve cells in case the neural network fell into a local minimum.Additionally,subordinate functions of each parameter were established to normalize the actual values of parameters of coalbed methane reservoirs in the range between zero and unity.Eventually, evaluation values of all coalbed methane reservoirs could be obtained by using the comprehensive evaluation method,which is the basis to rank the coalbed methane reservoirs in the order of exploitation priority.The greater the evaluation value,the higher the exploitation priority.The ranking method was verified in this paper by ten exploited coalbed methane reservoirs in China.The evaluation results are in agreement with the actual exploitation cases.The method can ensure the truthfulness and credibility of the weights of parameters and avoid the subjectivity caused by experts.Furthermore,the probability of falling into local minima is reduced,because genetic the algorithm is used to optimize the neural network system. | Li Weichao Wu Xiaodong Shi Junfeng | 2008 | Petroleum Science2008，5，4： | 4 |
| 10 | Secondary prevention medication persistence and prognosis of acute ischaemic stroke or transient ischaemic attack显示文摘Introduction The risk of disability and mortality is high among recurrent stroke,which highlights the importance of secondary prevention measures.We aim to evaluate medication persistence for secondary prevention and the prognosis of acute ischaemic stroke or transient ischaemic attack(TIA)in China.Methods Patients with acute ischaemic stroke or TIA from the China National Stroke Registry II were divided into 3 groups based on the percentage of persistence in secondary prevention medication classes from discharge to 3 months after onset(level I:persistence=0%,level II:0% | Lei Zhang Junfeng Shi Yuesong Pan Zixiao Li Hongyi Yan Chelsea Liu Wei Lv Xia Meng Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，3： | 3 |
| 11 | Orchestration of energy metabolism and osteogenesis by Mg^(2+)facilitates low-dose BMP-2-driven regeneration显示文摘The clinical application of bone morphogenetic protein-2(BMP-2)is limited by several factors,including ineffectiveness at low doses and severe adverse effects at high doses.To address these efficacy and safety limitations,we explored whether orchestration of energy metabolism and osteogenesis by magnesium ion(Mg^(2+))could reduce the dose and thereby improve the safety of BMP-2.Our results demonstrated that rapid metabolic activation triggered by BMP-2 was indispensable for subsequent osteogenesis.Moreover,inadequate metabolic stimulation was shown to be responsible for the ineffectiveness of low-dose BMP-2.Next,we identified that Mg^(2+),as an'energy propellant',substantially increased cellular bioenergetic levels to support the osteogenesis via the Akt-glycolysis-Mrs2-mitochondrial axis,and consequently enhanced the osteoinductivity of BMP-2.Based on the mechanistic discovery,microgel composite hydrogels were fabricated as low-dose BMP-2/Mg^(2+)codelivery system through microfluidic and 3D printing technologies.An in vivo study further confirmed that rapid and robust bone regeneration was induced by the codelivery system.Collectively,these results suggest that this bioenergetic-driven,cost-effective,low-dose BMP-2-based strategy has substantial potential for bone repair. | Sihan Lin Shi Yin Junfeng Shi Guangzheng Yang Xutao Wen Wenjie Zhang Mingliang Zhou Xinquan Jiang | 2022 | Bioactive Materials2022，7，12： | 2 |
| 12 | Structure-guided analysis of Arabidopsis JASMONATE-INDUCED OXYGENASE(JOX)2 reveals key residues for recognition of jasmonic acid substrate by plant JOXs显示文摘The jasmonic acid(JA)signaling pathway is used by plants to control wound responses.The persistent accumulation of JA inhibits plant growth,and the hydroxylation of JA to 12-hydroxy-JA by JASMONATE-INDUCED OXYGENASEs(JOXs,also named jasmonic acid oxidases)is therefore vital for plant growth,while structural details of JA recognition by JOXs are unknown.Here,we present the 2.65Åresolution X-ray crystal structure of Arabidopsis JOX2 in complex with its substrate JA and its co-substrates 2-oxoglutarate and Fe(Ⅱ).JOX2 contains a distorted double-stranded p helix(DSBH)core flanked by a helices and loops.JA is bound in the narrow substrate pocket by hydrogen bonds with the arginine triad R225,R350,and R354 and by hydrophobic interactions mainly with the phenylalanine triad F157,F317,and F346.The most critical residues for JA binding are F157 and R225,both from the DSBH core,which interact with the cyclopentane ring of JA.The spatial distribution of critical residues for JA binding and the shape of the substrate-binding pocket together define the substrate selectivity of the JOXs.Sequence alignment shows that these critical residues are conserved among JOXs from higher plants.Collectively,our study provides insights into the mechanism by which higher plants hydroxylate the hormone JA. | Xin Zhang Dongli Wang Joyce Elberse Linlu Qi Wei Shi You-Liang Peng Robert C.Schuurink Guido Van den Ackerveken Junfeng Liu | 2021 | Molecular Plant2021，14，5： | 2 |
| 13 | Safety and efficacy of meplazumab in healthy volunteers and COVID-19 patients: a randomized phase 1 and an exploratory phase 2 trial显示文摘Recent evidence suggests that CD147 serves as a novel receptor for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Blocking CD147 via anti-CD147 antibody could suppress the in vitro SARS-CoV-2 replication.Meplazumab is a humanized anti-CD147 IgG_(2) monoclonal antibody,which may effectively prevent SARS-CoV-2 infection in coronavirus disease 2019(COVID-19)patients.Here,we conducted a randomized,double-blinded,placebo-controlled phase 1 trial to evaluate the safety,tolerability,and pharmacokinetics of meplazumab in healthy subjects,and an open-labeled,concurrent controlled add-on exploratory phase 2 study to determine the efficacy in COVID-19 patients.In phase 1 study,59 subjects were enrolled and assigned to eight cohorts,and no serious treatment-emergent adverse event(TEAE)or TEAE grade≥3 was observed.The serum and peripheral blood Cmax and area under the curve showed non-linear pharmacokinetic characteristics.No obvious relation between the incidence or titer of positive anti-drug antibody and dosage was observed in each cohort.The biodistribution study indicated that meplazumab reached lung tissue and maintained>14 days stable with the lung tissue/cardiac blood-pool ratio ranging from 0.41 to 0.32.In the exploratory phase 2 study,17 COVID-19 patients were enrolled,and 11 hospitalized patients were involved as concurrent control.The meplazumab treatment significantly improved the discharged(P=0.005)and case severity(P=0.021),and reduced the time to virus negative(P=0.045)in comparison to the control group.These results show a sound safety and tolerance of meplazumab in healthy volunteers and suggest that meplazumab could accelerate the recovery of patients from COVID-19 pneumonia with a favorable safety profile. | Huijie Bian Zhao-Hui Zheng Ding Wei Aidong Wen Zheng Zhang Jian-Qi Lian Wen-Zhen Kang Chun-Qiu Hao Jing Wang Rong-Hua Xie Ke Dong Jie-Lai Xia Jin-Lin Miao Wen Kang Guoquan Li Di Zhang Mingru Zhang Xiu-Xuan Sun Likun Ding Kui Zhang Junfeng Jia Jin Ding Zhiqin Li Yanyan Jia Lin-Na Liu Zhe Zhang Zhao-Wei Gao Hong Du Na Yao Qing Wang Ke Wang Jie-Jie Geng Bin Wang Ting Guo Ruo Chen Yu-Meng Zhu Li-Juan Wang Qian He Rui-Rui Yao Ying Shi Xiang-Min Yang Jian-Sheng Zhou Yi-Nan Ma Ya-Tao Wang Xue Liang Fei Huo Zhe Wang Yang Zhang Xu Yang Ye Zhang Lu-Hua Gao Ling Wang Xiao-Chun Chen Hao Tang Shuang-Shuang Liu Qing-Yi Wang Zhi-Nan Chen Ping Zhu | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 14 | On-chip multiplexed single-cell patterning and controllable intracellular delivery显示文摘Conventional electroporation approaches show limitations in the delivery of macromolecules in vitro and in vivo.These limitations include low efficiency,noticeable cell damage and nonuniform delivery of cells.Here,we present a simple 3D electroporation platform that enables massively parallel single-cell manipulation and the intracellular delivery of macromolecules and small molecules.A pyramid pit micropore array chip was fabricated based on a silicon wet-etching method.A controllable vacuum system was adopted to trap a single cell on each micropore.Using this chip,safe single-cell electroporation was performed at low voltage.Cargoes of various sizes ranging from oligonucleotides(molecular beacons,22 bp)to plasmid DNA(CRISPR-Cas9 expression vectors,>9 kb)were delivered into targeted cells with a significantly higher transfection efficiency than that of multiple benchmark methods(e.g.,commercial electroporation devices and Lipofectamine).The delivered dose of the chemotherapeutic drug could be controlled by adjusting the applied voltage.By using CRISPR-Cas9 transfection with this system,the p62 gene and CXCR7 gene were knocked out in tumor cells,which effectively inhibited their cellular activity.Overall,this vacuum-assisted micropore array platform provides a simple,efficient,high-throughput intracellular delivery method that may facilitate on-chip cell manipulation,intracellular investigation and cancer therapy. | Zaizai Dong Yanli Jiao Bingteng Xie Yongcun Hao Pan Wang Yuanyuan Liu Junfeng Shi Chandani Chitrakar Stephen Black Yu-Chieh Wang LJames Lee Mo Li Yubo Fan Lingqian Chang | 2020 | Microsystems & Nanoengineering2020，6，1： | 2 |
| 15 | High Performance Supercapacitors from Hierarchical Porous Carbon Aerogels Based on Sliced Bread显示文摘 | Pin Hao Guanwei Cui Xifeng Shi Junfeng Xie Xinyuan Xia Yuanhua Sang C. P. Wong Hong Liu Bo Tang | 2017 | Chinese Journal of Chemistry2017，35，5： | 2 |
| 16 | Compound Gaoziban tablet(复方高滋斑片) alleviates depression via toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-kappa B pathway显示文摘OBJECTIVE: To evaluate the effect of compound Gaoziban tablet(复方高滋斑片, CGZBT) on depression, and to investigate the underlying mechanism. METHODS: The components of CGZBT were analysed by high-performance liquid chromatography. Then, we assessed the effects of varying doses of CGZBT on an established chronic unpredictable mild stress(CUMS) model in rats. Whether animals were depressed was evaluated by sucrose preference test, open field test and forced swimming test. Neurotransmitters of hippocampus were detected by liquid chromatography-mass spectrometry. Serum levels of tumor necrosis factor-alpha(TNF-α), interleukin(IL)-1β, IL-6, IL-4, and IL-10 were measured by enzyme-linked immunosorbent assay. Expressions of toll-like receptor 4(TLR4), myeloid differentiation factor 88(My D88), phospho-nuclear factorkappa B(p-NF-κB), cyclooxygenase-2(COX-2), ionized calcium binding adapter molecule-1(IBA-1) were assessed by immunohistochemical staining and western blotting. RESULTS: Eight compounds were identified from CGZBT, moreover, our results showed that CGZBT effectively reversed the CUMS-induced decrease in sucrose preference, shortened the movement distance and prolonged immobility time. CGZBT significantly increased levels of 5-hydroxytryptamine, dopamine, norepinephrine, 5-hydroxyindoleacetic acid levels, and reduced the expression of TNF-α, IL-1β, IL-6, yet increased IL-4 and IL-10. Furthermore, the expressions of TLR4, My D88, COX-2, p-NF-κB and IBA-1 in hippocampus were effectively reversed after treatment with CGZBT. CONCLUSIONS: These results indicated that CGZBT could, at least in part, alleviate depression induced by CUMS via the TLR4/My D88/NF-κB pathway, suggesting its potential as an antidepressant drug. | ZOU Xinshuang SHI Lei YIN Hailong LI Haiping WANG Mengheng SONG Wanci LUO Laichun WU Hezhen YANG Yanfang ZAN Junfeng LIU Yanwen DAN Hanxiong YIN Qiang YOU Pengtao | 2022 | Journal of Traditional Chinese Medicine2022，42，6： | 2 |
| 17 | The influence of core–shell structured modifiers on the toughness of poly (vinyl chloride)显示文摘 | Guangfeng Wu Junfeng Zhao Haitao Shi Huixuan Zhang | 2004 | European Polymer Journal2004，，11： | 1 |
| 18 | NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments显示文摘Seamount is a unique deep-sea ecosystem widely distributed in the world.Its biodiversity is vibrant due to its specific geographical and hydrological conditions.However,the diversity and features of foraminifera in such an environment have rarely been studied.We extracted environmental DNA(eDNA)in sedime nts and amplified the partial small subunit ribo somal DNA(SSU rDNA)of fo raminife ra to understand the foraminiferal diversity from four sites in Magellan Seamount(Western Pacific Ocean).Partial S SU rDNA sequencing was conducted and 912979 foraminiferal reads were obtained and gathered into 266 operational taxonomic units(OTUs).In the available dataset,a high proportion of rare OTUs and low identity OTUs in each studied sample showed that the Magellan Seamount foraminiferal community might have a high genetic novelty.The relative abundance of foraminifera varied between replicates probably due to the existence of bias in amplification process and patchiness of the deep-sea floor.It showed that the Magellan Seamount has a relatively high benthic foraminiferal diversity characterized mainly by monothalamiids(76.37%of total reads)in association with rotaliids(19.03%of total reads),including planktic foraminiferal sequences(38.58%of rotaliids;7.36%of total reads).The remaining reads were assigned to miliolids(0.83%of total reads)and textulariids(0.66%of total reads),and 3.11%of total reads are unassigned to a specific family.The co mparative analysis with foraminiferal assemblage s from coastal and deep-sea environme nts indicated that seamounts could aggregate species from a nearby deep-sea. | Junfeng SHI Yanli LEI Haotian LI Tiegang LI | 2021 | Journal of Oceanology and Limnology2021，39，5： | 1 |
| 19 | Inhibitory effect of CD147/HAb18 monoclonal antibody on cartilage erosion and synovitis in the SCID mouse model for rheumatoid rhthritis显示文摘 | Junfeng Jia Conghua Wang Zhangguo Shi | | 0，，： | 1 |
| 20 | Strontium-incorporated bioceramic scaffolds for enhanced osteoporosis bone regeneration显示文摘The restoration of bone defects caused by osteoporosis remains a challenge for surgeons.Strontium ranelate has been applied in preventative treatment approaches due to the biological functions of the trace element strontium(Sr).In this study,we aimed to fabricate bioactive scaffolds through Sr incorporation based on our previously developed modified amino-functional mesoporous bioactive glass(MBG)and to systematically investigate the bioactivity of the resulting scaffold in vitro and in vivo in an osteoporotic rat model.The results suggested that Sr-incorporated amino-functional MBG scaffolds possessed favorable biocompatibility.Moreover,with the incorporation of Sr,osteogenic and angiogenic capacities were upregulated in vitro.The in vivo results showed that the Sr-incorporated amino-functional MBG scaffolds achieved better bone regeneration and vessel formation.Furthermore,bioinformatics analysis indicated that the Sr-incorporated amino-functional MBG scaffolds could reduce reactive oxygen species levels in bone marrow mesenchymal stem cells in the osteoporotic model by activating the cAMP/PKA signaling pathway,thus playing an anti-osteoporosis role while promoting osteogenesis.This study demonstrated the feasibility of incorporating trace elements into scaffolds and provided new insights into biomaterial design for facilitating bone regeneration in the treatment of osteoporosis. | Qianju Wu Longwei Hu Ran Yan Junfeng Shi Hao Gu Yuwei Deng Ruixue Jiang Jin Wen Xinquan Jiang | 2022 | Bone Research2022，10，4： | 1 |
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