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| 1 | Marginal sealing around integral bilayer scaffolds for repairing osteochondral defects based on photocurable silk hydrogels显示文摘Osteochondral repair remains a major challenge in current clinical practice despite significant advances in tissue engineering.In particular,the lateral integration of neocartilage into surrounding native cartilage is a difficult and inadequately addressed problem that determines the success of tissue repair.Here,a novel design of an integral bilayer scaffold combined with a photocurable silk sealant for osteochondral repair is reported.First,we fabricated a bilayer silk scaffold with a cartilage layer resembling native cartilage in surface morphology and mechanical strength and a BMP-2-loaded porous subchondral bone layer that facilitated the osteogenic differentiation of BMSCs.Second,a TGF-β3-loaded methacrylated silk fibroin sealant(Sil-MA)exhibiting biocompatibility and good adhesive properties was developed and confirmed to promote chondrocyte migration and differentiation.Importantly,this TGF-β3-loaded Sil-MA hydrogel provided a bridge between the cartilage layer of the scaffold and the surrounding cartilage and then guided new cartilage to grow towards and replace the degraded cartilage layer from the surrounding native cartilage in the early stage of knee repair.Thus,osteochondral regeneration and superior lateral integration were achieved in vivo by using this composite.These results demonstrate that the new approach of marginal sealing around the cartilage layer of bilayer scaffolds with Sil-MA hydrogel has tremendous potential for clinical use in osteochondral regeneration. | Xiaolin Wu Mingliang Zhou Fei Jiang Shi Yin Sihan Lin Guangzheng Yang Yuezhi Lu Wenjie Zhang Xinquan Jiang | 2021 | Bioactive Materials2021，6，11： | 7 |
| 2 | Orchestration of energy metabolism and osteogenesis by Mg^(2+)facilitates low-dose BMP-2-driven regeneration显示文摘The clinical application of bone morphogenetic protein-2(BMP-2)is limited by several factors,including ineffectiveness at low doses and severe adverse effects at high doses.To address these efficacy and safety limitations,we explored whether orchestration of energy metabolism and osteogenesis by magnesium ion(Mg^(2+))could reduce the dose and thereby improve the safety of BMP-2.Our results demonstrated that rapid metabolic activation triggered by BMP-2 was indispensable for subsequent osteogenesis.Moreover,inadequate metabolic stimulation was shown to be responsible for the ineffectiveness of low-dose BMP-2.Next,we identified that Mg^(2+),as an'energy propellant',substantially increased cellular bioenergetic levels to support the osteogenesis via the Akt-glycolysis-Mrs2-mitochondrial axis,and consequently enhanced the osteoinductivity of BMP-2.Based on the mechanistic discovery,microgel composite hydrogels were fabricated as low-dose BMP-2/Mg^(2+)codelivery system through microfluidic and 3D printing technologies.An in vivo study further confirmed that rapid and robust bone regeneration was induced by the codelivery system.Collectively,these results suggest that this bioenergetic-driven,cost-effective,low-dose BMP-2-based strategy has substantial potential for bone repair. | Sihan Lin Shi Yin Junfeng Shi Guangzheng Yang Xutao Wen Wenjie Zhang Mingliang Zhou Xinquan Jiang | 2022 | Bioactive Materials2022，7，12： | 2 |
| 3 | CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro显示文摘Due to the low number of collectable stem cells from single umbilical cord blood(UCB)unit,their initial uses were limited to pediatric therapies.Clinical applications of UCB hematopoietic stem and progenitor cells(HSPCs)would become feasible if there were a culture method that can effectively expand HSPCs while maintaining their self-renewal capacity.In recent years,numerous attempts have been made to expand human UCB HSPCs in vitro.In this study,we report that caffeic acid phenethyl ester(CAPE),a small molecule from honeybee extract,can promote in vitro expansion of HSPCs.Treatment with CAPE increased the percentage of HSPCs in cultured mononuclear cells.Importantly,culture of CD34+HSPCs with CAPE resulted in a significant increase in total colony-forming units and high proliferative potential colony-forming units.Burst-forming unit-erythroid was the mostly affected colony type,which increased more than 3.7-fold in 1μg mL 1CAPE treatment group when compared to the controls.CAPE appears to induce HSPC expansion by upregulating the expression of SCF and HIF1-α.Our data suggest that CAPE may become a potent medium supplement for in vitro HSPC expansion. | LIU YiMing ZHANG BoWen ZHANG Jing WANG SiHan YAO HaiLei HE LiJuan CHEN Lin YUE Wen LI YanHua PEI XueTao | 2014 | Science China(Life Sciences)2014，57，2： | 2 |
| 4 | Capacity analysis for indoor PLC using different multi-carrier modulation schemes 显示文摘 | Lin H Sihan P | 2010 | IEEE Transactions on Power Delivery2010，25，1： | 1 |
| 5 | Preservation of alveolar ridge height through mechanical memory: A novel dental implant design显示文摘Irreversible marginal bone loss can hinder recovery around dental implants.Insufficient alveolar osteogenesis and stress concentration during chewing contribute to marginal bone resorption and can result in implant failure.A biomaterial with a micropore-channel structure was developed using 3D printing technology.This design facilitated bony ingrowth and provided similar mechanical stimulation at the implant neck during mastication to a natural tooth.The micropore channels provided a guiding structure for bone mesenchymal stem cell proliferation and differentiation without the need for growth factors.Specifically,this was achieved through mechanical transduction by F-actin remodeling and the activation of Yes-associated protein(YAP).The implants were verified in a canine dental implant surgery model,which demonstrated the promising use of biomaterialbased dental implants in future clinical applications. | Shi Yin Wenjie Zhang Yanmei Tang Guangzheng Yang Xiaolin Wu Sihan Lin Xuanyong Liu Huiliang Cao Xinquan Jiang | 2021 | Bioactive Materials2021，6，1： | 1 |
| 6 | Study of UV-Bleached channel-waveguide performance in nonlinear optical polymer films显示文摘 | Wei Feng Sihan Lin Brian booker R | 1996 | Appl Opt1996，34，30： | 1 |
| 7 | Modeling photobleached optical polymer waveguides 显示文摘 | Jiong Ma Sihan Lin Wei Feng | 1995 | Appl Opt1995，34，24： | 1 |
| 8 | Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models显示文摘A two-dimensional(2D)cell culture-based model is widely applied to study tumorigenic mechanisms and drug screening.However,it cannot authentically simulate the threedimensional(3D)microenvironment of solid tumors and provide reliable and predictable data in response to in vivo,thus leading to the research ilusionis and failure of drug screening.In this study,honeycomb-like gelatin methacryloyl(GelMA)hydrogel microspheres are developed by synchronous photocrosslinking microluidic technique to construct a 3D model of osteosarcoma.The in vitro study shows that ostcosarcoma cells(K7M2)culured in 3D GelMA microspheres have stronger tumorous stemness。proliferation and migration abilitics,more osteoclastogenetic ability,and reistance to chemotherapeutic drugs(DOX)than that of cells in 2D cultures.More imporantly,the 3D-cultured K7M2 cells show more tumorigenicity in immunologically sound mice,characterized by shorter tumorigenesis time,larger tumor volumc,severe bone destruction,and higher mortality.In conclusion,honeycomb like porous microsphere scaffolds are constructed with uniform structure by micofluidic technology to massively produce tumor cells with original phenotypes.Those microspheres could recapitulate the physiology microenvironment of tumors.maintain ell-cell and cell-extracellular matrix interactions,and thus provide an efective and convenient strategy for tumor pathogenesis and drug screening research. | Jiachen He Chichi Chen Liang Chen Ruoyu Cheng Jie Sun Xingzhi Liu Lin Wang Can Zhu Sihan Hu Yuan Xue Jian Lu Huiling Yang Wenguo Cui Qin Shi | 2022 | Research2022，，2： | 1 |
| 9 | Multifunctional composite nanoparticles:Magnetic,luminescent,and mesoporous显示文摘 | Lin Yushen Wu Sihan Hung Yann | 2006 | Chem Mater2006，18，： | 1 |
| 10 | cAMP-PGC1α途径诱导的抗Warburg效应驱动胶质母细胞瘤细胞分化为星形胶质细胞显示文摘文章简介多形性胶质母细胞瘤(GBM)是人类最具侵袭性的肿瘤之一。虽然分化治疗已被提出作为治疗GBM的潜在方法,但诱导分化的机制仍然很不清楚。在此,课题组利用c AMP激活剂建立了GBM诱导分化模型,该模型特异性地指导GBM分化为星形胶质细胞。 | Fan Xing Yizhao Luan Jing Cai Sihan Wu Jialuo Mai Jiayu Gu Haipeng Zhang Kai Li Yuan Lin Xiao Xiao Jiankai Liang Yuan Li Wenli Chen Yaqian Tan Longxiang Sheng Bingzheng Lu Wanjun Lu Mingshi Gao Pengxin Qiu Xingwen Su Wei Yin Jun Hu Zhongping Chen Ke Sai Jing Wang Furong Chen Yinsheng Chen Shida Zhu Dongbing Liu Shiyuan Cheng 谢志 朱文博 颜光美 | 2018 | 科学新闻2018，0，4： | 1 |
| 11 | Therapeutic effect of Qinghuayin(清化饮)against chronic atrophic gastritis through the inhibition of toll or interleukin-1 receptor domain-containing adaptor inducing interferon-β signaling pathway显示文摘OBJECTIVE:To examine the efficacy of Qinghuayin(清化饮,QHY)in rat chronic atrophic gastritis(CAG)models and explored the molecular mechanism of QHY in treating CAG.METHODS:In total,65 Wistar rats were randomly divided into the control(n=10)and CAG groups(n=55).CAG model rats were further divided into five groups:model(n=10),vitacoenzyme(n=10),low-dose QHY(n=10),medium-dose QHY(n=10),and high-dose QHY groups(n=10).We analyzed histopathological changes using hematoxylin and eosin staining and measured interleukin(IL)-6 and IL-8 levels in serum using enzyme-linked immunosorbent assay(ELISA)(Boster Bio,Pleasanton,USA).In addition,gastrin(GAS),pepsinogen I(PGI),and PGII expressions were evaluated using ELISA.The protein and m RNA expression of toll-like receptor 4(TLR4)and toll or interleukin-1 receptor domaincontaining adaptor inducing interferon-β(TRIF)was detected by Western blotting and quantitative reverse transcription-polymerase chain reaction,respectively.RESULTS:Our results revealed that histopathological changes in CAG model rates could be restored by low-,medium-,and high-dose QHY.The changes in GAS and PGI/II expression demonstrated that QHY improved CAG.Serum IL-6 and IL-levels were decreased by QHY administration.TLR4 and TRIF were upregulated at the m RNA and protein levels in the model group but downregulated by QHY administration.CONCLUSION:We concluded that QHY could effectively improve the histopathological changes of the gastric mucosa induced by CAG in rats.The therapeutic mechanism of QHY may be related to inhibition of the inflammatory factors IL-6 and IL-8 and suppression of TLR4/TRIF m RNA and protein expression. | HE Youcheng YAO Xiaoling LI Sihan ZHOU Hongjian XU Ruoying ZHENG Rong XIAO Wendi SU Ning LIN Ping HUANG Minghan | 2022 | Journal of Traditional Chinese Medicine2022，42，2： | 1 |
| 12 | J-comb: An image fusion algorithm to combine observations covering different spatial frequency ranges显示文摘Ground-based, high-resolution bolometric(sub)millimeter continuum mapping observations on spatially extended target sources are often subject to significant missing fluxes. This hampers accurate quantitative analyses. Missing flux can be recovered by fusing high-resolution images with observations that preserve extended structures. However, the commonly adopted image fusion approaches do not maintain the simplicity of the beam response function and do not try to elaborate the details of the yielded beam response functions. These make the comparison of the observations at multiple wavelengths not straightforward. We present a new algorithm, J-comb, which combines the high and low-resolution images linearly. By applying a taper function to the low-pass filtered image and combining it with a high-pass filtered image using proper weights, the beam response functions of our combined images are guaranteed to have near-Gaussian shapes. This makes it easy to convolve the observations at multiple wavelengths to share the same beam response functions. Moreover, we introduce a strategy to tackle the specific problem that the imaging at 850 μm from the present-date ground-based bolometric instrument and that taken with the Planck satellite do not overlap in the Fourier domain. We benchmarked our method against two other widely-used image combination algorithms, CASA-feather and MIRIAD-immerge, with mock observations of star-forming molecular clouds. We demonstrate that the performance of the J-comb algorithm is superior to those of the other two algorithms. We applied the J-comb algorithm to real observational data of the Orion A star-forming region. We successfully produced dust temperature and column density maps with ~10′′angular resolution, unveiling much greater details than the previous results. A py Thon code release of J-comb and implementation of the algorithm are available at http://gffzz188fe103f8f1460asqqn5bbwv0f0x69k0.ffgz.tsg.suse.edu.cn/Sihan Jiao/J-comb. | Sihan Jiao Yuxin Lin Xiangyu Shui Jingwen Wu Zhiyuan Ren Di Li | 2022 | Science China(Physics,Mechanics & Astronomy)2022，65，9： | 0 |
| 13 | Control of lupus activity during pregnancy via the engagement of IgG sialylation:novel crosstalk between IgG sialylation and pDC functions显示文摘Immunoglobulin(IgG)glycosylation affects the effector functions of IgG in a myriad of biological processes and has been closely associated with numerous autoimmune diseases,including systemic lupus erythematosus(SLE),thus underlining the pathogenic role of glycosylation aberration in autoimmunity.This study aims to explore the relationship between IgG sialylation patterns and lupus pregnancy.Relative to that in serum samples from the control cohort,IgG sialylation level was aberrantly downregulated in serum samples from the SLE cohort at four stages(from preconception to the third trimester of pregnancy)and was significantly associated with lupus activity and fetal loss during lupus pregnancy.The type I interferon signature of pregnant patients with SLE was negatively correlated with the level of IgG sialylation.The lack of sialylation dampened the ability of IgG to suppress the functions of plasmacytoid dendritic cells(pDCs).RNA-seq analysis further revealed that the expression of genes associated with the spleen tyrosine kinase(SYK)signaling pathway significantly differed between IgG-and deSia-IgG-treated pDCs.This finding was confirmed by the attenuation of the ability to phosphorylate SYK and BLNK in deSia-IgG.Finally,the coculture of pDCs isolated from pregnant patients with SLE with IgG/deSia-IgG demonstrated the sialylation-dependent anti-inflammatory function of IgG.Our findings suggested that IgG influences lupus activity through regulating pDCs function via the modulation of the SYK pathway in a sialic acid-dependent manner. | You Wang Sihan Lin Jiayue Wu Meng Jiang Jianhua Lin Yu Zhang Huihua Ding Haibo Zhou Nan Shen Wen Di | 2023 | Frontiers of Medicine2023，17，3： | 0 |
| 14 | Regulating macrophage-MSC interaction to optimize BMP-2-induced osteogenesis in the local microenvironment显示文摘Bone morphogenetic protein(BMP-2)has been approved by the FDA to promote bone regeneration,but uncertain osteogenic effect and dose-dependent side effects may occur.Osteoimmunomodulation plays an important role in growth factor-based osteogenesis.Here,we explored how proinflammatory signals affect the dose-dependent osteogenic potential of BMP-2.We observed that the expression level of local IL-1βdid not increase with the dose of BMP-2 in the mouse osteogenesis model.A low dose of BMP-2 could not promote new bone formation,but trigger the release of IL-1βfrom M1 macrophages.As the dose of BMP-2 increased,the IL-1βexpression and M1 infiltration in local microenvironment were inhibited by IL-1Ra from MSCs under osteogenic differentiation induced by BMP-2,and new bone tissues formed,even excessively.Anti-inflammatory drugs(Dexamethasone,Dex)promoted osteogenesis via inhibiting M1 polarization and enhancing BMP-2-induced MSC osteo-differentiation.Thus,we suggest that the osteogenic effect of BMP-2 involves macrophage-MSC interaction that is dependent on BMP-2 dose and based on IL-1R1 ligands,including IL-1βand IL-1Ra.The dose of BMP-2 could be reduced by introducing immunoregulatory strategies. | Fei Jiang Xuanyu Qi Xiaolin Wu Sihan Lin Junfeng Shi Wenjie Zhang Xinquan Jiang | 2023 | Bioactive Materials2023，，7： | 0 |
| 15 | Can Turbulent, High-density Gas Form Stars in Molecular Clouds: A Case Study in Ophiuchus显示文摘Star formation is governed by the interplay between gravity and turbulence in most of molecular clouds.Recent theoretical works assume that dense gas,whose column density is above a critical value in the column density probability distribution function(N-PDF),where gravity starts to overcome turbulence,becomes star-forming gas and will collapse to form stars.However,these high-density gases will include some very turbulent areas in the clouds.Will these dense but turbulent gases also form stars?We test this scenario in Ophiuchus molecular cloud using N-PDF analysis and find that at least in some regions,the turbulent,dense gas is not forming stars.We identified two isolated high-density structures in Ophiuchus,which are gravitationally unbound and show no sign of star formation.Their high densities may come from turbulence. | Sihan Jiao Jingwen Wu Hao Ruan Yuxin Lin Chao-Wei Tsai Linjing Feng | 2022 | Research in Astronomy and Astrophysics2022，22，7： | 0 |
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