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| 1 | Adsorption behavior of phosphate on Lanthanum(Ⅲ) doped mesoporous silicates material显示文摘A series of lanthanum doped meosoporous MCM-41(LaxM41,x is Si/La molar ratio) was prepared by sol-gel method.The surface structure of the materials was investigated with X-ray diffraction and N2 adsorption/desorption technique.The content of La in the materials was determined by ICP.It was found that the La content of La25M41,La50M41 and La100M41 was 7.53%,3.89% and 2.32%,respectively.The phosphate adsorption capacities increased with increasing amount of La incorporation.With 0.40 g La25M41 99.7% phosphate could be removed.The effects of Si/La molar ratio,LaxM41 dose,pH,initial concentration of phosphate solution,co-ions on phosphate adsorption were also evaluated.The phosphate adsorption kinetics of LaxM41 could be well-described by the pseudo second-order model,and Langmuir isotherm fit equilibrium data much better than the Freundlich isotherm. | Jianda Zhang Zhemin Shen Wenpo Shan Ziyan Chen Zhijian Mei Yangming Lei Wenhua Wang | 2010 | Journal of Environmental Sciences2010，22，4： | 18 |
| 2 | Metal chlorides loaded on activated carbon to capture elemental mercury显示文摘Activated carbon (AC) was considered to be an effective sorbent to control mercury in combustion systems. However, its capture capacity was low and it required a high carbon-to-mercury mass ratio. AC loaded with catalyst showed a high elemental mercury (Hg 0 ) capture capacity due to large surface area of AC and high oxidization ability of catalyst. In this study, several metal chlorides and metal oxides were used to promote the sorption capacity of AC. As a result, metal chlorides were better than metal oxides loaded on AC to remove gaseous mercury. X-ray diffractometer (XRD), thermogravimetric analyzer (TGA) and specific surface area by Brunauer- Emmett-Teller method (BET) analysis showed the main mechanisms: first, AC had an enormous surface area for loading enough MClx; second, Cl and MxOy were generated during pyrogenation of MClx; finally, there were lots of active elements such as Cl and MxOy which could react with elemental mercury and convert it to mercury oxide and mercury chloride. The HgO and HgCl 2 might be released from AC's porous structure by thermo regeneration. A catalytic chemisorption mechanism predominates the sorption process of elemental mercury. As Co and Mn were valence variable metal elements, their catalytic effect on Hg 0 oxidization may accelerate both oxidation and halogenation of Hg 0 . The sorbents loaded with metal chlorides possessed a synergistic function of catalytic effect of valence variable metal and chlorine oxidation. | Zhemin Shen,Jing Ma,Zhijian Mei,Jianda Zhang School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240,China | 2010 | Journal of Environmental Sciences2010，22，11： | 18 |
| 3 | Control techniques of tilt rotor unmanned aerial vehicle systems: A review显示文摘The tilt rotor unmanned aerial vehicle(TRUAV) exhibits special application value due to its unique rotor structure. However, varying dynamics and aerodynamic interference caused by tiltable rotors are great technical challenges and key issues for TRUAV's high-powered flight controls, which have attracted the attention of many researchers. This paper outlines the concept of TRUAV and some typical TRUAV platforms while focusing on control techniques. TRUAV structural features, dynamics modeling, and flight control methods are discussed, and major challenges and corresponding developmental tendencies associated with TRUAV flight control are summarized. | Liu Zhong He Yuqing Yang Liying Han Jianda | 2017 | Chinese Journal of Aeronautics2017，30，1： | 17 |
| 4 | Anticancer immunotherapy by CTLA-4 blockade: obligatory contribution of IL-2 receptors and negative prognostic impact of soluble CD25显示文摘堵住抗体 ipilimumab 的细胞毒素的 T 淋巴细胞 antigen-4 (CTLA-4 ) 在很少的病人导致变形黑瘤的调停免疫者的长期的控制。尽管 ipilimumab 无疑经由 immunostimulation 施加它的治疗学的效果,这样远的临床上有用的、 immunologically 相关的 biomarkers 预言治疗效率是逃犯的。这里,我们显示出 IL-2 的那中立化或堵住 α并且 βIL-2 受体的子单元(CD25 和 CD122,分别地) 否则在现出症状之前的潜的老鼠模型由 CTLA-4 封锁导致了,废除了 antitumor 效果和 intratumoral T 受动器对规章的房间(Tregs ) 的比率的伴随的改进,它是。CTLA-4 封锁导致了在失去了 FoxP3 表示并且在 regressing 肿瘤积累了的 IL-2-producing 受动器房间与伴随物上升表示 Lag3, ICOS, IL-10 和 Egr2 的一个镇压 CD4 + T 房间子集的减小。当 recombinant IL-2 改进了 CTLA-4 封锁的治疗学的功效时,圈套 IL-2 受体 α(IL-2Rα, sCD25 ) 禁止了 CTLA-4 的 anticancer 效果封锁。在收到 ipilimumab 的 262 个变形黑瘤病人, sCD25 的基线浆液集中代表了全面幸存的独立指示物,与预言到治疗的抵抗的高水平。总的来说,这些结果解开为在 CTLA-4 的 anticancer 活动的 IL-2 和 IL-2 受体的一个角色封锁。重要地,我们的学习提供第一 immunologically 相关的 biomarker,也就是提高的浆液 sCD25,那与黑瘤在病人预言抵抗到 CTLA-4 封锁。 | Dalil Hannani Marie Vetizou David Enot Sylvie Rusakiewicz Nathalie Chaput David Klatzmann Melanie Desbois Nicolas Jacquelot Nadege Vimond Salem Chouaib Christine Mateus James P Allison Antoni Ribas Jedd D Wolchok Jianda Yuan Philip Wong Michael Postow Andrzej Mackiewicz Jacek Mackiewicz Dirk Schadendorff Dirk Jaeger Alan J Korman Keith Bahjat Michele Maio Luana Calabro Michele WL Teng Mark J Smyth Alexander Eggennont Caroline Robert Guido Kroemer Laurence Zitvogel | 2015 | Cell Research2015，25，2： | 14 |
| 5 | STUDY ON SENSOR-BASED CONTROL METHOD FOR HARMONIC DRIVE SYSTEM显示文摘A new type of robust traje ctory tracking control for harmonic using joint torque sensor and joint acceleration sensor information is concerned with.Joint torque sensor information is used to compensate the uncertainty of link and load parameters. Joint acceleration feedback control will enhace the robustness of the driving system, resist the dynamic uncertainties and disturbing torque acted on the joint axis within definite bandwidth, improve the joint tracking performance, and resist the vibration of the load side of the harmonic drive system. Experimental studies are carried out and comparison of several controllers , such as PD and sensor- based control, the experimental results clearly illustrate the effectiveness of the proposed methods. | Tan Dalong, Qiu Zhicheng, Han Jianda (Shenyang Institute of Automation, Chinese Academy of Sciences) | 2000 | Chinese Journal of Mechanical Engineering2000，13，3： | 12 |
| 6 | Simple PID Parameter Tuning Method Based on Outputs of the Closed Loop System显示文摘Most of the existing PID parameters tuning methods are only effective with pre-known accurate system models, which often require some strict identification experiments and thus infeasible for many complicated systems. Actually, in most practical engineering applications, it is desirable for the PID tuning scheme to be directly based on the input-output response of the closed-loop system. Thus, a new parameter tuning scheme for PID controllers without explicit mathematical model is developed in this paper. The paper begins with a new frequency domain properties analysis of the PID controller. After that, the definition of characteristic frequency for the PID controller is given in order to study the mathematical relationship between the PID parameters and the open-loop frequency properties of the controlled system. Then, the concepts of M-field and θ-field are introduced, which are then used to explain how the PID control parameters influence the closed-loop frequency-magnitude property and its time responses. Subsequently, the new PID parameter tuning scheme, i.e., a group of tuning rules, is proposed based on the preceding analysis. Finally, both simulations and experiments are conducted, and the results verify the feasibility and validity of the proposed methods. This research proposes a PID parameter tuning method based on outputs of the closed loop system. | HAN Jianda ZHU Zhiqiang JIANG Ziya HE Yuqing | 2016 | Chinese Journal of Mechanical Engineering2016，29，3： | 12 |
| 7 | Outcomes in refractory diffuse large B-cell lymphoma:results fromamulticenter real-world study in China显示文摘Background:Diffuse large B-cell lymphoma(DLBCL)patients refractory to rituximab-based immunochemotherapy have a dismal prognosis.However,the definition of refractory DLBCL remains inconsistent and no large cohort study data is available from Asian countries.To validate the definition and outcomes of refractory DLBCL in China,we conducted a multicenter,retrospective cohort study.Methods:The REtrospective AnaLysis of Treatment REspoNse of refractory DLBCL(REAL-TREND)study was performed using real-world data from 8 centers in China.DLBCL patients with curative intent were included in the REAL-TREND dataset.Overall survival(OS)was estimated using the Kaplan-Meier method and compared by the log-rank test.Due to heterogeneity in response rates among different centers,the response rates of refractory patients were pooled using random-effect models.Multivariate survival analysis was performed using the Cox regression model.Results:A total of 2778 DLBCL patients diagnosed between January,2010 and December,2015 were enrolled to this study.After validating previous definitions,the SCHOLAR-1 study was most suitable to define refractory DLBCL.The estimated 5-year cumulative incidence of refractory patients was 20%(95% confidence Interval[CI]=18%-22%).After the determination of refractory disease,overall response rate and complete remission rate were 30%(95%CI=22%-38%)and 9%(95%CI=4%-15%),respectively.Patients with either no response to immunochemotherapy or relapse within 12 months after stem-cell transplantation had inferior survival with a median OS of 5.9 months(95%CI=5.5-7.1 months)and 2-year OS rate of 16%(95%CI=12%-20%).International prognostic index score 4-5(hazard ratio[HR]=2.22;95%CI=1.47-3.35),central nervous systemrelapse(HR=1.43;95%CI=1.04-1.97),and best response status(HR=2.68;95%CI=1.42-5.03 for partial remission.HR=5.97,95%CI=3.21-11.11 for stable disease/progressive disease)were independent unfavorable prognostic factors.Conclusions:This is the first large-scale Asian cohort study focusing on outcomes of refractory DLBCL.The definition of the SCHOLAR-1 study identifies patients with homogenously inferior survival,thus is appropriate to select refractory DLBCL.Due to poor clinical outcomes in the rituximab era,patients with refractory DLBCL may be potential candidates for novel treatment modalities. | Shuo Wang Li Wang Jianda Hu Wenbin Qian Xi Zhang Yu Hu Qi Zhu Bobin Chen Depei Wu Chung-Chou H.Chang Pengpeng Xu Xiaoyun Zheng Juying Wei Yao Liu Guohui Cui Yong Tang Yan Ma Haiwen Huang Hongmei Yi Weili Zhao | 2021 | Cancer Communications2021，41，3： | 10 |
| 8 | Clinical risk score for invasive fungal diseases in patients with hematological malignancies undergoing chemotherapy:China Assessment of Antifungal Therapy in Hematological Diseases (CAESAR) study显示文摘Invasive fungal disease (IFD) is a major infectious complication in patients with hematological malignancies.In this study,we examined 4889 courses of chemotherapy in patients with hematological diseases to establish a training dataset (n=3500) by simple random sampling to develop a weighted risk score for proven or probable IFD through multivariate regression,which included the following variables: male patients,induction chemotherapy for newly diagnosed or relapsed disease,neutropenia,neutropenia longer than 10 days,hypoalbuminemia,central-venous catheter,and history of IFD.The patients were classified into three groups,which had low (0-10,~1.2%),intermediate (11-15,6.4%),and high risk (> 15,17.5%) of IFD.In the validation set (n=1389),the IFD incidences of the groups were ~1.4%,5.0%,and 21.4%.In addition,we demonstrated that antifungal prophylaxis offered no benefits in low-risk patients,whereas benefits were documented in intermediate (2.1% vs.6.6%,P=0.007) and high-risk patients (8.4% vs.23.3%,P=0.007).To make the risk score applicable for clinical settings,a pre-chemo risk score that deleted all unpredictable factors before chemotherapy was established,and it confirmed that anti-fungal prophylaxis was beneficial in patients with intermediate and high risk of IFD.In conclusion,an objective,weighted risk score for IFD was developed,and it may be useful in guiding antifungal prophylaxis. | Ling Wang Ying Wang Jiong Hu Yuqian Sun He Huang Jing Chen Jianyong Li Jun Ma Juan Li Yingmin Liang Jianmin Wang Yan Li Kang Yu Jianda Hu Jie Jin Chun Wang Depei Wu Yang Xiao Xiaojun Huang | 2019 | Frontiers of Medicine2019，13，3： | 10 |
| 9 | Long-term joint effect of nutrients and temperature increase on algal growth in Lake Taihu,China显示文摘To study how global warming and eutrophication affect water ecosystems,a multiplicative growth Monod model,modified by incorporating the Arrhenius equation,was applied to Lake Taihu to quantitatively study the relationships between algal biomass and both nutrients and temperature using long-term data.To qualitatively assess which factor was a limitation of the improved model,temperature variables were calculated using annual mean air temperature (AT),water temperature (WT),and their average temperature (ST),while substrate variables were calculated using annual mean total nitrogen (TN),total phosphorus (TP),and their weighted aggregate (R),respectively.The nine fitted curves showed that TN and AT were two important factors influencing algal growth;AT limited growth as algal photosynthesis is mainly carried out near the water surface;N leakage of phytoplankton and internal phosphorus load from sediment explains why TN was the best predictor of peak biomass using the Monod model.The fitted results suggest that annual mean algal biomass increased by 0.145 times when annual mean AT increased by 1.0°C.Results also showed that the more eutrophic the lake,the greater the effect AT had on algal growth.Subsequently,the long-term joint effect of annual temperature increase and eutrophication to water ecosystems can be quantitatively assessed and predicted. | Chun Ye Zhemin Shen Tao Zhang Maohong Fan Yangming Lei Jianda Zhang | 2011 | Journal of Environmental Sciences2011，23，2： | 9 |
| 10 | NONLINEAR ESTIMATION METHODS FOR AUTONOMOUS TRACKED VEHICLE WITH SLIP显示文摘以便完成追踪的车辆的精确、柔韧的自治指导和控制,一个运动学的模型与纵、侧面滑倒被建立。四个不同非线性的过滤器被用来估计两个联合说向量和创造模型的变化时间的参数向量。第一个过滤器是著名扩大 Kalman 过滤器。第二个过滤器是 Kalman 过滤器的一个 unscented 版本。第三个是用 unscentedKalman 过滤器的一个粒子过滤器产生重要性建议分发。最后一个是使用一个线性集合会员评估者的一个新奇、保证的过滤器并且能给真状态躺在的一个椭圆体集合。四条不同途径有被调查并且比较的不同复杂性,行为和优点。 | ZHOU Bo HAN Jianda | 2007 | Chinese Journal of Mechanical Engineering2007，20，4： | 8 |
| 11 | Rapid growth of a long-seed KDP crystal显示文摘To reduce the seed length while maintaining the advantages of the cuboid KDP-type crystal,a long-seed KDP crystal with size 471 mm×480 mm×400 mm is rapidly grown.With almost the same high cutting efficiency to obtain third harmonic generation oriented samples,this long-seed KDP-type crystal can be grown with a shorter seed than that of the cuboid KDP-type crystal.The full width at half maximum of the high-resolution X-ray diffraction of the(200)crystalline face is 28.8 arc seconds,indicating that the long-seed KDP crystal has good crystalline quality.In the wavelength range of 377–1022 nm,the transmittance of the long-seed KDP crystal is higher than 90%.The fluence for the 50%probability of laser-induced damage(LID)is 18.5 J/cm^2(3 ns,355 nm).Several test points survive when the laser fluence exceeds 30 J/cm^2(3 ns,355 nm),indicating the good LID performance of the long-seed KDP crystal.At present,the growth of a long-seed DKDP crystal is under way. | Duanyang Chen Bin Wang Hu Wang Xiangyu Zhu Ziyuan Xu Yuanan Zhao Shenghao Wang Kaizao Ni Lili Zheng Hui Zhang Hongji Qi Jianda Shao | 2020 | High Power Laser Science and Engineering2020，8，1： | 5 |
| 12 | Backstepping Based Global Exponential Stabilization of a Tracked Mobile Robot with Slipping Perturbation显示文摘 | Bo Zhou Jianda Han Xianzhong Dai | 2011 | Journal of Bionic Engineering2011，8，1： | 4 |
| 13 | ONLINE MODEL AND ACTUATOR FAULT TOLERANT CONTROL FOR AUTONOMOUS MOBILE ROBOT显示文摘A novel fault-tolerant adaptive control methodology against the actuator faults is proposed. The actuator effectiveness factors (AEFs) are introduced to denote the healthy of actuator, and the unscented Kalman filter (UKF) is employed for online estimation of both the motion states and the AEFs of mobile robot. A square root version of the UKF is introduced to improve efficiency and numerical stability. Using the information from the UKF, the reconfigurable controller is designed automatically based on an enhancement inverse dynamic control (IDC) methodology. The experiment on a 3-DOF omni-directional mobile robot is performed, and the effectiveness of the proposed method is demonstrated. | SONG Qi JIANG Zhe HAN Jianda | 2007 | Chinese Journal of Mechanical Engineering2007，20，3： | 4 |
| 14 | Removal of phosphate by Fe-coordinated amino-functionalized 3D mesoporous silicates hybrid materials显示文摘Phosphate removal from aqueous waste streams is an important approach to control the eutrophication downstream bodies of water.A Fe(III) coordinated amino-functionalized silicate adsorbent for phosphate adsorption was synthesized by a post-grafting and metal cation incorporation process.The surface structure of the adsorbent was characterized by X-ray diffraction,N2 adsoropion/desoprotion technique,and Fourier transform infrared spectroscopy.The experimental results showed that the adsorption equilibrium data were well fitted to the Langmuir equation.The maximum adsorption capacity of the modified silicate material was 51.8 mg/g.The kinetic data from the adsorption of phosphate were fitted to pseudo second-order model.The phosphate adsorption was highly pH dependent and the relatively high removal of phosphate fell within the pH range 3.0-6.0.The coexistence of other anions in solutions has an adverse effect on phosphate adsorption;a decrease in adsorption capacity followed the order of exogenous anions:F > SO2-4 > NO-3 > Cl-.In addition,the adsorbed phosphate could be desorbed by NaOH solutions.This silicate adsorbent with a large adsorption capacity and relatively high selectivity could be utilized for the removal of phosphate from aqueous waste streams or in aquatic environment. | Jianda Zhang Zhemin Shen Zhijian Mei Shanping Li Wenhua Wang | 2011 | Journal of Environmental Sciences2011，23，2： | 4 |
| 15 | Detection of collapsed buildings with the aerial images captured from UAV显示文摘In this paper, we present a method of detecting the collapsed buildings with the aerial images which are captured by an unmanned aerial vehicle(UAV) for the postseismic evaluation. Different from the conventional methods that apply the satellite images or the high-altitude UAV for the coarse disaster evaluation over large area, the purpose of this work is to achieve the accurate detection of collapsed buildings in small area from low altitude. By combining the motion and appearance features of collapsed buildings extracted from successive aerial images, each pixel in the input image will be measured by a statistical method where the background pixels will be penalized and the ones of collapsed buildings will be assigned with high value.The candidates of collapsed buildings will be established by integrating the extracted feature points into local groups with the online clustering algorithm. To reduce the false alarm caused by the complex background noise,each predicted candidate will be further verified by the temporal tracking framework where both the trajectory and the appearance of a candidate will be measured. The candidate of collapsed buildings that can survive through long time will be considered as true positive, otherwise rejected as a false alarm. Through extensive experiments, the efficiency and the effectiveness of proposed algorithm have been proved. | Chunsheng HUA Juntong QI Hong SHANG Weijian HU Jianda HAN | 2016 | Science China(Information Sciences)2016，59，3： | 3 |
| 16 | Multi-walled carbon nanotubes covalently functionalized by axially coordinated metal-porphyrins: Facile syntheses and temporally dependent optical performance显示文摘向轴的方向协调了 metal-porphyrin-functionalized 多围的碳 nanotube (MWCNT ) nanohybrids 经由二条不同合成途径(一个壶 1,3-dipolar cycloaddition 反应和那深奥 1,3-dipolar cycloaddition 由亲核的替换跟随了的一条逐步的途径) 被准备,并且通过分光镜的技术描绘了。到 MWCNT 的表面的听卟啉的附件显著地改进他们处理的溶解度和容易。协调的这些向轴的方向(5,10,15,20-tetraphenylporphyrinato ) 锡(IV )(SnTPP )-MWCNTs 展览重要荧光熄灭。结果的 nanohybrids 的第三顺序的非线性的光性质被与毫微秒和微微秒激光脉搏在 532 nm 使用 Z 扫描技术学习。当分别地,毫微秒或微微秒脉搏被采用时,结果证明 nanohybrids 展出重要反向的可饱和的吸收或可饱和的吸收。在毫微秒政体的改进非线性的吸收在继续到 nanohybrids 上被观察并且被归功于到 MWCNT 和化学上依附的金属卟啉的突出的性质的联合。 | Aijian Wang Jingbao Song Zhipeng Huang Yinglin Song Wang Yu Huanli Dong Wenping Hu Marie P. Cifuentes Mark G. Humphrey Long Zhang Jianda Shao Chi Zhang | 2016 | Nano Research2016，9，2： | 3 |
| 17 | Analysis of electromagnetic propagation in birefringent thin film显示文摘In general, the propagating behavior of extraordinary wave in anisotropic materials is different from that in isotropic materials. With the tangential continuity of Maxwell’s equations, the electromagnetic propagating behaviors have been investigated at the incident and exit interfaces of the uniaxial anisotropic thin film. The emphasis was placed on two interesting optical phenomena such as homolateral refraction behavior and wide-angle Brewster’s phenomenon, which occurred at the interfaces of uniaxial anisotropic thin film. | QI Hongji WANG Jianguo SHAO Jianda FAN Zhengxiu | 2005 | Science China(Physics,Mechanics & Astronomy)2005，48，5： | 3 |
| 18 | sEMG based quantitative assessment of acupuncture on Bell's palsy:an experimental study显示文摘Acupuncture is a traditional Chinese therapeutic method, recognized by western medicine as an important complementary therapy. However, the efficacy assessment of this traditional intervention has been mainly empirical rather than scientific. In this paper, we propose a surface electromyography(s EMG) based approach for quantitative assessment of acupuncture on Bell's palsy. The assessment is made by comparing the muscle activities of healthy with diseased ones. A feature-vector of four individual s EMG features is introduced for more comprehensive representation of muscle activity. A clustering technique is then proposed to calculate quantitative differences between the muscle activities of the healthy and diseased sides. A regressive model is also proposed to predict the recovery trend of a patient based on the quantitative assessments during his/her clinical acupuncture history. As reported in the paper, a total of 20 selected Bell's palsy patients have participated in the extensive experiments that were conducted to verify the performance of the proposed method. | HAN JianDa XIONG AnBin ZHAO XinGang DING QiChuan CHEN YiGuo LIU GuangJun | 2015 | Science China(Information Sciences)2015，58，8： | 3 |
| 19 | Clonal isolation of endothelial colony-forming cells from early gestation chorionic villi of human placenta for fetal tissue regeneration显示文摘BACKGROUND Endothelial colony-forming cells(ECFCs)have been implicated in the process of vascularization,which includes vasculogenesis and angiogenesis.Vasculogenesis is a de novo formation of blood vessels,and is an essential physiological process that occurs during embryonic development and tissue regeneration.Angiogenesis is the growth of new capillaries from pre-existing blood vessels,which is observed both prenatally and postnatally.The placenta is an organ composed of a variety of fetal-derived cells,including ECFCs,and therefore has significant potential as a source of fetal ECFCs for tissue engineering.AIM To investigate the possibility of isolating clonal ECFCs from human early gestation chorionic villi(CV-ECFCs)of the placenta,and assess their potential for tissue engineering.METHODS The early gestation chorionic villus tissue was dissociated by enzyme digestion.Cells expressing CD31 were selected using magnetic-activated cell sorting,and plated in endothelial-specific growth medium.After 2-3 wks in culture,colonies displaying cobblestone-like morphology were manually picked using cloning cylinders.We characterized CV-ECFCs by flow cytometry,immunophenotyping,tube formation assay,and Dil-Ac-LDL uptake assay.Viral transduction of CVECFCs was performed using a Luciferase/tdTomato-containing lentiviral vector,and transduction efficiency was tested by fluorescent microscopy and flow cytometry.Compatibility of CV-ECFCs with a delivery vehicle was determined using an FDA approved,small intestinal submucosa extracellular matrix scaffold.RESULTS After four passages in 6-8 wks of culture,we obtained a total number of 1.8×107 CV-ECFCs using 100 mg of early gestational chorionic villus tissue.Immunophenotypic analyses by flow cytometry demonstrated that CV-ECFCs highly expressed the endothelial markers CD31,CD144,CD146,CD105,CD309,only partially expressed CD34,and did not express CD45 and CD90.CV-ECFCs were capable of acetylated low-density lipoprotein uptake and tube formation,similar to cord blood-derived ECFCs(CB-ECFCs).CV-ECFCs can be transduced with a Luciferase/tdTomato-containing lentiviral vector at a transduction efficiency of 85.1%.Seeding CV-ECFCs on a small intestinal submucosa extracellular matrix scaffold confirmed that CV-ECFCs were compatible with the biomaterial scaffold.CONCLUSION In summary,we established a magnetic sorting-assisted clonal isolation approach to derive CV-ECFCs.A substantial number of CV-ECFCs can be obtained within a short time frame,representing a promising novel source of ECFCs for fetal treatments. | Kewa Gao Siqi He Priyadarsini Kumar Diana Farmer Jianda Zhou Aijun Wang | 2020 | World Journal of Stem Cells2020，12，2： | 2 |
| 20 | Bioactive extracellular matrix scaffolds engineered with proangiogenic proteoglycan mimetics and loaded with endothelial progenitor cells promote neovascularization and diabetic wound healing显示文摘Diabetic ischemic wound treatment remains a critical clinical challenge.Neovascularization plays a significant role in wound healing during all stages of the tissue repair process.Strategies that enhance angiogenesis and neovascularization and improve ischemic pathology may promote the healing of poor wounds,particularly diabetic wounds in highly ischemic conditions.We previously identified a cyclic peptide LXW7 that specifically binds to integrinαvβ3 on endothelial progenitor cells(EPCs)and endothelial cells(ECs),activates vascular endothelial growth factor(VEGF)receptors,and promotes EC growth and maturation.In this study,we designed and synthesized a multi-functional pro-angiogenic molecule by grafting LXW7 and collagen-binding peptides(SILY)to a dermatan sulfate(DS)glycosaminoglycan backbone,named LXW7-DS-SILY,and further employed this multi-functional molecule to functionalize collagen-based extracellular matrix(ECM)scaffolds.We confirmed that LXW7-DS-SILY modification significantly promoted EPC attachment and growth on the ECM scaffolds in vitro and supported EPC survival in vivo in the ischemic environment.When applied in an established Zucker Diabetic Fatty(ZDF)rat ischemic skin flap model,LXW7-DS-SILY-functionalized ECM scaffolds loaded with EPCs significantly improved wound healing,enhanced neovascularization and modulated collagen fibrillogenesis in the ischemic environment.Altogether,this study provides a promising novel treatment to accelerate diabetic ischemic wound healing,thereby reducing limb amputation and mortality of diabetic patients. | Siqi He Tanaya Walimbe Hongyuan Chen Kewa Gao Priyadarsini Kumar Yifan Wei Dake Hao Ruiwu Liu Diana L.Farmer Kit S.Lam Jianda Zhou Alyssa Panitch Aijun Wang | 2022 | Bioactive Materials2022，7，4： | 2 |
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