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| 1 | Fluctuations in butyrate-producing bacteria in ulcerative colitis patients of North India显示文摘AIM: To study the interplay between butyrate concentration and butyrate-producing bacteria in fecal samples of ulcerative colitis (UC) patients vs control individuals. METHODS: Fecal samples were collected from 14 control individuals (hemorrhoid patients only) and 26 UC patients (severe: n = 12, moderate: n = 6, remission: n = 8), recruited by the gastroenterologist at the Department of Gastroenterology, All India Institute of Medical Sciences, New Delhi, India. Disease activity in UC patients was determined by clinical colitis activity index. We employed fluorescent in situ hybridization in combination with flow cytometry to enumerate the clostridium cluster population targeted by 16S rRNA gene probe. Major butyrate-producing species within this cluster were quantified to see if any change existed in control vs UC patients with different disease activity. This observed change was further validated by quantitative polymerase chain reaction. In addition to this,we carried out gas chromatography to evaluate the changes in concentration of major short chain fatty acids (SCFAs), namely acetate, n -butyrate, iso -butyrate, in the above samples. Student t test and Graph pad prism-6 were used to compare the data statistically. RESULTS: There was a significant decrease of Clostridium coccoides (control, 25.69% ± 1.62% vs severe, 9.8% ± 2.4%, P = 0.0001) and Clostridium leptum clusters (control, 13.74% ± 1.05% vs severe, 6.2% ± 1.8%, P = 0.0001) in fecal samples of UC patients. Furthermore, we demonstrated that some butyrateproducing members of the clostridial cluster, like Fecalibacterium prausnitzii (control, 11.66% ± 1.55% vs severe, 6.01% ± 1.6%, P = 0.0001) and Roseburia intestinalis (control, 14.48% ± 1.52% vs severe, 9% ± 1.83%, P = 0.02) were differentially present in patients with different disease activity. In addition, we also demonstrated decreased concentrations of fecal SCFAs, especially of n -butyrate (control, 24.32 ± 1.86 mmol/μL vs severe, 12.74 ± 2.75 mmol/μL, P = 0.003), iso -butyrate (control, 1.70 ± 0.41 mmol/μL vs severe, 0.68 ± 0.24 mmol/μL, P = 0.0441) and acetate (control, 39.51 ± 1.76 mmol/μL vs severe, 32.12 ± 2.95 mmol/ μL,P = 0.047), in the fecal samples of UC patients. The observed decrease of predominant butyrate producers of clostridial clusters correlated with the reduced SCFA levels in active UC patients. This was further confirmed by the restoration in the population of some butyrate producers with simultaneous increase in the level of SCFA in remission samples. CONCLUSION: Our observations indicate that decreases in members of the clostridial cluster resulting in reduced butyrate levels contribute to the etiology of UC. | Reena Kumari Vineet Ahuja Jaishree Paul | 2013 | World Journal of Gastroenterology2013，19，22： | 21 |
| 2 | MicroRNA exhibit altered expression in the inflamed colonic mucosa of ulcerative colitis patients显示文摘AIM To investigate the miRNA expression in colonic mucosal biopsies from endoscopically inflamed and non inflamed regions of ulcerative colitis(UC) patients. METHODS Colonic mucosal pinch biopsies were analyzed from the inflamed and non inflamed regions of same UC patient. Total RNA was isolated and differential miRNA profiling was done using microarray platform. Quantitative Real Time PCR was performed in colonic biopsies from inflamed(n = 8) and non-inflamed(n = 8) regions of UC and controls(n = 8) to validate the differential expression of miRNA. Potential targets of dysregulated miRNA were identified by using in silico prediction tools and probable role of these miRNA in inflammatory pathways were predicted.RESULTS The miRNA profile of inflamed colonic mucosa differs significantly from the non-inflamed. Real time PCR analysis showed that some of the miRNA were differentially expressed in the inflamed mucosa as compared to non inflamed mucosa and controls(mi R-125 b, mi R-223, miR-138, and mi R-155), while(miR-200a) did not show any significant changes. In contrast to microarray, where mi R-378 d showed downregulation in the inflamed mucosa, q RT-PCR showed a significant upregulation in the inflamed mucosa as compared to the non inflamed. The in silico prediction analysis revealed that the genes targeted by these mi RNAs play role in the major signaling pathways like MAPK pathway, NF-κB signaling pathway, cell adhesion molecules which are all assciated with UC.CONCLUSION The present study reports disease specific alteration in the expression of mi R-125 b, mi R-155, mi R-223 and mi R-138 in UC patients and also predict their biological significance. | Swati Valmiki Vineet Ahuja Jaishree Paul | 2017 | World Journal of Gastroenterology2017，23，29： | 6 |
| 3 | Principles of cancer treatment by chem- otherapy显示文摘 | Jaishree Bhosle Geoff Hall | 2009 | Cancer Treatment2009，27，4： | 1 |
| 4 | Application of Immunohistochemistry to the Diagnosis of Primary and Metastatic Carcinoma to the Lung显示文摘 | Jagirdar Jaishree | 2008 | Archives of Pathology & Laboratory Medicine2008，，3： | 1 |
| 5 | Antinociceptive activity of swertiamarin isolated from Enicostemma axillare 显示文摘 | Jaishree V Badami S Rupesh Kumar M | 2009 | Phytomedicine2009，16，23： | 1 |
| 6 | Antinociceptive activity of swertiamarin isolated from Enicostemma axillare显示文摘 | V. Jaishree S. Badami M. Rupesh Kumar T. Tamizhmani | 2008 | Phytomedicine2008，，2： | 1 |
| 7 | In vitro antioxidant properties of new thiazole derivatives显示文摘 | V. Jaishree N. Ramdas J. Sachin B. Ramesh | 2011 | Journal of Saudi Chemical Society2011，，4： | 1 |
| 8 | A simple speetrophotometric determination of endosulfan in river water and soil显示文摘 | JAISHREE Raju GUPTA V K | 1991 | Freseniust Journal of Analytical Chemistry1991，339，： | 1 |
| 9 | TLR4 D299G Polymorphism Modulates Cytokine Expression in Ulcerative Colitis显示文摘 | Naresh K. Meena Ravi Verma Nirmal Verma Vineet Ahuja Jaishree Paul | 2013 | Journal of Clinical Gastroenterology2013，，9： | 1 |
| 10 | Nonablative faeelift in Indian skin with superpulsed radiofrequency显示文摘 | Jaishree Sharad | 2011 | Indian Dermatol Online J2011，2，： | 1 |
| 11 | Glycolic acid peel therapy-a current re- view显示文摘 | Jaishree Sharad | 2013 | Clin Cosmet Investig Dermatol2013，11，6： | 1 |
| 12 | Diverting the tourists: a spatial decision-support system for tourism planning on a developing island显示文摘 | Jaishree Beedasy | 1999 | Spatial Decision Support System for Tourism Site Selection1999，，3： | 1 |
| 13 | Principles of cancer treatment bychemotherapy显示文摘 | Jaishree Bhosle Geoff Hall | 2009 | Cancer Treatment2009，27，4： | 1 |
| 14 | Probiotic Efficien- cy of Spirulina platensis - Stimulating Growth of Lactic Acid Bacte- ria显示文摘 | Dola Bhowmik Jaishree Dubey Sandeep Mehra | 2009 | World Journal of dairy & food sciences2009，4，2： | 1 |
| 15 | Principles of cancer treatment by chemotherapy显示文摘 | Jaishree Bhosle Geoff Hall | 2009 | Cancer Treatment2009，27，4： | 1 |
| 16 | 显示文摘 | Raja Babu Panwar Rajeev Gupta Bal Kishan Gupta Sadiq Raja Jaishree Vaishnav Meenakshi Khatri Aachu Agrawal Atherothrombotic risk factors & premature coronary heart disease in India: a case-control study | 2011 | The Indian journal of medical research2011，，134： | 1 |
| 17 | Disseminated cryptosporidiosis:Case report and literature review显示文摘Cryptosporidiosis,better known as an intestinal disease may disseminate to infect other sites including the respiratory tract. Little information however is available on respiratory cryptosporidiosis that may largely be due to lower frequency of respiratory cryptosporidiosis. Respiratory cryptosporidiosis has been majorly reported in immunocompromised individuals and children. Here we report a case of respiratory and intestinal cryptosporidiosis in a fifteen months old child with CD8+ deficiency. The patient in spite of treatment with Nitazoxanide and Azithromycin followed by Intravenous immunoglobulin and Bovine colostrum had a fatal outcome. The Cryptosporidium spp. isolate was subjected to molecular characterization. The Cryptosporidium spp. was identified both in stool specimen and Endotracheal aspirate(ETA). The blood sample was negative for Cryptosporidium spp. The Cryptosporidium spp. isolate from stool as well as ETA was identified as Cryptosporidium hominis(C. hominis) using Multiplex Allele Specific Polymerase Chain Reaction assay and was subtyped as Ia A23G1R1 subtype using gp60 gene polymerase chain reaction assay followed by sequencing. | Shehla Khalil Bijay R Mirdha Jaishree Paul Ashutosh Panda Yogita Singh | 2017 | World Journal of Clinical Infectious Diseases2017，7，2： | 1 |
| 18 | TLR4 D299G Polymorphism Modulates Cytokine Expression in Ulcerative Colitis显示文摘 | Naresh K. Meena Ravi Verma Nirmal Verma Vineet Ahuja Jaishree Paul | 2013 | Journal of Clinical Gastroenterology2013，，9： | 1 |
| 19 | Comparison of stool concentration methods for detection of prevalence of enteroparasitic infection in rural tertiary care teaching hospital of Maharashtra显示文摘 | Jaishree Puri Nilekar SL | 2014 | Indian Journal of Basic and Applied Medical Research2014，3，4： | 1 |
| 20 | Antioxidant and hepatoprotective effect of swertiamarin from Enicostemma axillare against D-galactosamine induced aeute liver damage in rats 显示文摘 | Jaishree V Badami S | 2009 | J Ethnopharmacol2009，130，1： | 1 |
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