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| 1 | Punctual mutations in 23S rRNA gene of clarithromycinresistant Helicobacter pylori in Colombian populations显示文摘AIM To characterize punctual mutations in 23S rRNA gene of clarithromycin-resistant Helicobacter pylori(H. pylori) and determine their association with therapeutic failure.METHODS PCR products of 23S rRNA gene V domain of 74 H. pylori isolates; 34 resistant to clarithromycin(29 from a low-risk gastric cancer(GC) population: TumacoColombia, and 5 from a high-risk population: TuquerresColombia) and 40 from a susceptible population(28 from Tumaco and 12 from Túquerres) were sequenced using capillary electrophoresis. The concordance between mutations of V domain 23S rRNA gene of H. pylori and therapeutic failure was determined using the Kappa coefficient and Mc Nemar's test was performed to determine the relationship between H. pylori mutationsand clarithromycin resistance.RESULTS23S rRNA gene from H. pylori was amplified in 56/74 isolates, of which 25 were resistant to clarithromycin(20 from Tumaco and 5 from Túquerres, respectively). In 17 resistant isolates(13 from Tumaco and 4 from Túquerres) the following mutations were found: A1593 T1, A1653 G2, C1770 T, C1954 T1, and G1827 C in isolates from Tumaco, and A2144 G from Túquerres. The mutations T2183 C, A2144 G and C2196 T in H. pylori isolates resistant to clarithromycin from Colombia are reported for the first time. No association between the H. pylori mutations and in vitro clarithromycin resistance was found. However, therapeutic failure of eradication treatment was associated with mutations of 23S rRNA gene in clarithromycin-resistant H. pylori(κ = 0.71).CONCLUSION The therapeutic failure of eradication treatment in the two populations from Colombia was associated with mutations of the 23S rRNA gene in clarithromycinresistant H. pylori. | Andrés Jenuer Matta Diana Carolina Zambrano Alvaro Jairo Pazos | 2018 | World Journal of Gastroenterology2018，24，14： | 5 |
| 2 | Molecular and in vitro Characterization of Field Isolates of Bovine Herpesvirus-1显示文摘Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines. | Julian Ruiz-Saenz Jairo Jaime Gloria Ramirez Victor Vera | 2012 | Virologica Sinica2012，27，1： | 4 |
| 3 | Carbon Isotope Discrimination is not Correlated with Transpiration Efficiency in Three Cool-Season Grain Legumes(Pulses)显示文摘叶子的碳同位素辨别(tp#pdC ) 被显示了在大量种类与叶子的蒸发效率被相关。这导致了在引起节目为改进蒸发效率选择被使用的 tp#pdC。在 tp#pdC 和蒸发效率之间的关联在小扁豆(透镜 culinaris Medikus ) 的六遗传型在生育相期间在浇得好的条件下面被决定,嘴豆(Cicer arietinum L.) 的六遗传型和狭窄叶的植物(Lupinus 狭叶的的 L.) 的 10 栽培变种。在所有三种和蒸发效率在遗传型之中改变的生物资源(干物质) 累积和水使用(蒸发) 更高是 40% ~ 75% 在与最不有效的遗传型相比最有效。然而, tp#pdC 和蒸发效率显著地没在任何种类被相关。这建议 tp#pdC 技术不能在学习的三条豆科粮食罪(脉搏) 为蒸发效率在选择被使用。 | Neil C.Turner Jairo A.Palta Renuka Shrestha Christiane Ludwig Kadambot H.M.Siddique David W.Turner | 2007 | Journal of Integrative Plant Biology2007，49，10： | 3 |
| 4 | Genomic variability of Helicobacter pylori isolates of gastric regions from two Colombian populations显示文摘AIM To compare the genomic variability and the multiple colonization of Helicobacter pylori(H. pylori) in patients with chronic gastritis from two Colombian populations with contrast in the risk of developing gastric cancer(GC): Túquerres-Nari?o(High risk) and Tumaco-Nari?o(Low risk).METHODS Four hundred and nine patients from both genders with dyspeptic symptoms were studied. Seventy-two patients were included in whom H. pylori was isolated from three anatomic regions of the gastric mucosa,(31/206) of the high risk population of GC(Túquerres) and(41/203) of the low risk population of GC(Tumaco). The isolates were genotyped by PCR-RAPD. Genetic diversity between the isolates was evaluated by conglomerates analysis and multiple correspondence analyses. RESULTS The proportion of virulent genotypes of H. pylori was 99% in Túquerres and 94% in Tumaco. The coefficient of similarity of Nei-Li showed greater genetic diversityamong isolates of Túquerres(0.13) than those of Tumaco(0.07). After adjusting by age, gender and type of gastritis, the multiple colonization was 1.7 times more frequent in Túquerres than in Tumaco(P = 0.05).CONCLUSION In Túquerres, high risk of GC there was a greater probability of multiple colonization by H. pylori. From the analysis of the results of the PCR-RAPD, it was found higher genetic variability in the isolates of H. pylori in the population of high risk for the development of GC. | Andrés Jenuer Matta Alvaro Jairo Pazos Javier Andrés Bustamante-Rengifo Luis Eduardo Bravo | 2017 | World Journal of Gastroenterology2017，23，5： | 2 |
| 5 | An Inactivated Vaccine from a Field Strain of Bovine Herpesvirus-1(BoHV-1) has High Antigenic Mass and Induces Strong Efficacy in a Rabbit Model显示文摘Bovine Herpesvirus-1 (BoHV-1) is a DNA virus belonging to the family Herpesviridae, subfamily Alfaherpesvirinae; it is a worldwide pathogen, causing serious economic losses in livestock. In Colombia there have been multiple isolates of BoHV-1 that have been subjected to molecular characterization, classifying most of the country isolates as BoHV-1.1. In the present study we developed and evaluated an ethyleneimine binary inactivated isolate from the native BoHV-1 strain (Córdoba-2) in a rabbit model of vaccination and infection. The vaccine was evaluated in two phases, one of immunogenicity with vaccination and a booster after 21 days, and an evaluation phase of protection against challenge with a highly virulent reference strain. The results demonstrate optimum serum-conversion, with protective neutralizing antibody titers 28 days post vaccination and optimal protection against challenge with the reference strain with decreased clinical signs of infection, protection against the onset of fever and decrease of virus excretion post challenge. In conclusion, our results show the enormous potential that an immunogenic inactivated vaccine has produced from the native BoHV-1.1 strain, which produces a high antigen mass to the vaccine to induce optimal immunity and protection, and it is a strong candidate for evaluation and possible future use in different cattle populations. | Julian Ruiz-Sáenz Jairo Jaime Victor Vera | 2013 | Virologica Sinica2013，28，1： | 2 |
| 6 | Effect of low level laser therapy on dental pulp during orthodontic movement显示文摘AIM: To validate the protocol described here to be used in future clinical trials related to the effect of laser therapy on dental pulp. METHODS: Histologically treated samples from eight human healthy premolar teeth obtained from the middle root level were distributed in four groups: group 1(G1) absolute control; group 2(G2) only laser irradiation; group 3(G3) exposed only to orthodontics; and group 4(G4) treated with orthodontics and laser. Laser treatment was performed at 830 nm wavelength, 100 mW(energy 80 J/cm2, 2.2 J), for 22 s in the vestibular surface and 22 s in the palatal surface, 1 mm away from the dental root mucosa. Three staining methods were performed: hematoxylin-eosin(HE), Masson's Trichrome method and Gomori's method.RESULTS: The pulp histology parameters were evaluated and the results classified in to 3 parts: an inflammatory response, soft tissue response(dental pulp) and hard tissue response(dentin and predentin). There was no inflammation(chronic or acute) in any of the evaluated groups. The zones of pulp necrosis were found in one premolar of G3 and in one of G4; in groups G2 and G4 there was higher angiogenesis than in the other two groups. G4 group presented the highest level of vascularization. A reduced nerve density was observed in G3. A G2 specimen showed increased nerve density. A higher rate of calcification was observed in G1 compared to G2. Denticles, either real or false, were observed in G1, G2 and G3. Sclerosis of dentin and focal dentin loss was observed among all the groups. Secondary dentin was present in one sample in G1 and G2. A necrosis zone was found in one sample of G3 and G4. No differences between groups were observed in the odontoblast irregularity layer but the layer was wider in the group treated with laser only. A notable difference was detected in reduction of the cell-free layer between the groups G1 and G4. The findings in pulp tissue favor its adaptative response against dental movement induced by orthodontics. No definitive conclusions may be derived as this is a pilot study. CONCLUSION: The protocol described here was shown to be an effective method to evaluate changes in dental pulp submitted to low level laser in teeth under orthodontic movement. | ngela Domínguez Rosa Emilia Ballesteros Jairo Hernán Viáfara Oscar Mario Tamayo | 2013 | World Journal of Methodology2013，3，2： | 2 |
| 7 | Sampling volatile compounds from natural products with headspace/solid-phase micro-extraction显示文摘 | Elena E. Stashenko Jairo R. Martínez | 2006 | Journal of Biochemical and Biophysical Methods2006，，2： | 2 |
| 8 | Design Optimization and Weight Reduction of 500 mL CSD PET Bottle through FEM Simulations显示文摘 | Carlos Alberto Silva De Miranda Jairo Jose Drummond Camara Olavo Pena Monken Claudio Gouvea Dos Santos | 2011 | 材料科学与工程(中英文B版)2011，1，7： | 2 |
| 9 | Exploiting dynamic reaction cell inductively coupled plasma mass spectrometry (DRC-ICP-MS) for sequential determination of trace elements in blood using a dilute-and-shoot procedure显示文摘 | Bruno Lemos Batista Jairo Lisboa Rodrigues Juliana Andrade Nunes Vanessa Cristina de Oliveira Souza Fernando Barbosa | 2009 | Analytica Chimica Acta2009，，1： | 1 |
| 10 | Paroxysmal atrioventricular block: clinical experience with 20 patients显示文摘 | Ronit Shohat-Zabarski Zaza Iakobishvili Jairo Kusniec Alexander Mazur Boris Strasberg | 2004 | International Journal of Cardiology2004，，3： | 1 |
| 11 | Comparison of two culture media for determination of the copper resistance of Xanthomonas strains and their usefulness for prediction of control with copper bactericides显示文摘 | Ken Pernezny Russell Nagata Nikol Havranek Jairo Sanchez | 2007 | Crop Protection2007，，2： | 1 |
| 12 | Martfnez SPME determination of volatile aldehydes for evaluation of in - vitro antioxidant activity 显示文摘 | Elena E Stashenko Miguel A Puertas Jairo R | 2002 | Anal Bioanal Chem2002，373，： | 1 |
| 13 | Spin hall effect transistor显示文摘 | Jtrg Wunderlich Byong-Guk Park Jairo Sinova | 2010 | Science2010，330，6012： | 1 |
| 14 | Structuralism and the Applicability of Mathematics 显示文摘 | Jairo Joseda Silva | 2010 | Axiomathes2010，20，： | 1 |
| 15 | Anomalous Hall effect 显示文摘 | Naoto Nagaosa Jairo Sinova Shigeki Onoda MacDon- ald A H Ong N P | 2010 | Re- views of Modern Physics2010，82，2： | 1 |
| 16 | Improvement of antitumor activity by gene amplification with a replicating but nondisseminating adenovirus显示文摘 | Denis Bourbeau Cara Jean Lau Jairo Jaime | 2007 | Cancer Res2007，67，7： | 1 |
| 17 | Morbidity and Mortality Associated With Nosocomial Transmission of Oseltamivir- Resistant Influenza A ( H1N1 ) Virus 显示文摘 | Jairo Gooskens Marcel Jonges Eric C J | 2009 | JAMA2009，301，10： | 1 |
| 18 | Martinez SPME determination of volatile aldehydes for evaluation of in - vitro antioxidant activity 显示文摘 | Elena E Stashenko Miguel A Puertas Jairo R | 2002 | Anal Bioanal Chem2002，373，： | 1 |
| 19 | Recent industrial applications of lignin:A sustainable ahemative to nonrenewable materials 显示文摘 | JAIRO H L WOLFGANG G G | 2002 | Journal of Polymers and the Environment2002，10，： | 1 |
| 20 | A fast ultrasound-as- sisted extraction procedure for trace elements determi- nation in hair samples by ICP-MS for foresic analysis显示文摘 | Bruno Lemos Batista Jairo Lisboa Rodrigues Vanessa Cristina de Oliveira Souza | 2009 | Forensic Science International2009，192，： | 1 |
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