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| 1 | Increased virulence of the oral microbiome in oral squamous cell carcinoma revealed by metatranscriptome analyses显示文摘Oral squamous cell carcinoma(OSCC) is the most prevalent and most commonly studied oral cancer. However, there is a void regarding the role that the oral microbiome may play in OSCC. Although the relationship between microbial community composition and OSCC has been thoroughly investigated, microbial profiles of the human microbiome in cancer are understudied. Here we performed a small pilot study of community-wide metatranscriptome analysis to profile mRNA expression in the entire oral microbiome in OSCC to reveal molecular functions associated with this disease. Fusobacteria showed a statistically significantly higher number of transcripts at tumour sites and tumour-adjacent sites of cancer patients compared to the healthy controls analysed. Regardless of the community composition, specific metabolic signatures were consistently found in disease. Activities such as iron ion transport, tryptophanase activity, peptidase activities and superoxide dismutase were over-represented in tumour and tumour-adjacent samples when compared to the healthy controls. The expression of putative virulence factors in the oral communities associated with OSCC showed that activities related to capsule biosynthesis, flagellum synthesis and assembly, chemotaxis, iron transport, haemolysins and adhesins were upregulated at tumour sites. Moreover, activities associated with protection against reactive nitrogen intermediates, chemotaxis, flagellar and capsule biosynthesis were also upregulated in non-tumour sites of cancer patients. Although they are preliminary, our results further suggest that Fusobacteria may be the leading phylogenetic group responsible for the increase in expression of virulence factors in the oral microbiome of OSCC patients. | Susan Yost Philip Stashenko Yoonhee Choi Maria Kukuruzinska Caroline A.Genco Andrew Salama Ellen O.Weinberg Carolyn D.Kramer Jorge Frias-Lopez | 2018 | International Journal of Oral Science2018，10，4： | 9 |
| 2 | Interleukin-1 and estrogen protect against disseminating dentoalveolar infections显示文摘Dentoalveolar bacterial infections cause localized tissue and bone destruction, but usually remain well-localized within teeth in immunocompetent hosts. However, in certain cases these infections may invade head and neck tissues, resulting in orofacial abscesses, cellulitis and sepsis, with resultant high morbidity and even mortality. In the present studies, we developed a novel model of spreading dentoalveolar infections in mice by treatment with neutralizing antibodies against both interleukin-1α(IL-1α)and IL-1β. Surprisingly male but not female mice given anti-IL-1 antibodies developed orofacial abscesses, weight loss,splenomegaly and sepsis. Female mice developed abscesses and sepsis comparable to males following ovariectomy(OVX), which was reversed by estrogen supplementation. Anti-IL-1 blockade inhibited IL-12, interferon γ(IFNγ) and IL-6 but not IL-10 expression in infrabony lesions, suggestive of a local anti-inflammatory response. There was greater infiltration of neutrophils and other inflammatory cells into lesions in anti-IL-1-treated animals; however, blood leukocytes had reduced bacterial phagocytic and killing activity ex vivo. Estrogen directly stimulated IL-1 production by macrophages, suggesting that the resistance of females to disseminating dentoalveolar infections may be due to their heightened pro-inflammatory responses following bacterial challenge, leading to enhanced localization of these infections. | hesham youssef philip stashenko | 2017 | International Journal of Oral Science2017，9，1： | 7 |
| 3 | Repellent activity of essential oils: A review显示文摘 | Luz Stella Nerio Jesus Olivero-Verbel Elena Stashenko | 2009 | Bioresource Technology2009，，1： | 2 |
| 4 | Repellent activity of essential oils: A review显示文摘 | Luz Stella Nerio Jesus Olivero-Verbel Elena Stashenko | 2009 | Bioresource Technology2009，，1： | 2 |
| 5 | Sampling volatile compounds from natural products with headspace/solid-phase micro-extraction显示文摘 | Elena E. Stashenko Jairo R. Martínez | 2006 | Journal of Biochemical and Biophysical Methods2006，，2： | 2 |
| 6 | Characterization of a human B lymphocyte-specific antigen显示文摘 | Nsdler L M Hardy R | 1980 | J Immunol1980，125，7： | 1 |
| 7 | Repellent activity of essential oils: a review 显示文摘 | Nerio LS Olivero-Verbel J Stashenko E | 2010 | Bioresour Technol2010，101，1： | 1 |
| 8 | Determination of volatile aldehydes forevaluafionof in vitro antioxidant activity 显示文摘 | Elena E Stashenko M A Puertas J R | 2002 | Anal Bioanal Chem2002，373，： | 1 |
| 9 | Expression of cell surface markers after human B lymphocyte activation显示文摘 | Nsdler L M Hardy R | 1981 | Proc Natl Acad Sci USA1981，78，3： | 1 |
| 10 | Monitoring volatile chemicals formed from must during yeast fermentation显示文摘 | Stashenko H Maeku C Shibamoto T | 1992 | J Agric Food Chem1992，40，11： | 1 |
| 11 | Bone-resorptive cytokine gene expression in periapical lesions in the rat显示文摘 | Wang CY Tani-lshii N Stashenko P | 1997 | Oral Microbiol Immunol1997，12，2： | 1 |
| 12 | Sampling volatile compounds from natural products with headspace/solid-phase micro-extrac tion显示文摘 | Stashenko E E Martinez J R | 2007 | Journal o{ Biochemical and Biophysical Methods2007，70，2： | 1 |
| 13 | Characterization of a human B lymphocyte-specific antigen 显示文摘 | Stashenko P Nadler LM Hardy R | 1980 | J Immunol1980，125，4： | 1 |
| 14 | Toll-like receptor 4-deficient mice have reduced bone destruction followingmixed anaerobic infection 显示文摘 | Hou L Sasaki H Stashenko P | 2000 | Infect Immun2000，68，8： | 1 |
| 15 | Martfnez SPME determination of volatile aldehydes for evaluation of in - vitro antioxidant activity 显示文摘 | Elena E Stashenko Miguel A Puertas Jairo R | 2002 | Anal Bioanal Chem2002，373，： | 1 |
| 16 | Comparative study of colombian citrus oils by high-resolution gas chromatography and gas chromatography-mass spectrometry显示文摘 | BLANCO TIRADO C STASHENKO EE Combariza MY | 1995 | J Chromatogr A1995，697，12： | 1 |
| 17 | Essential oils with insecticidal activity against larvae of Aedes aegypti (Diptera: Culicidae)显示文摘 | Sharon Smith Vera Diego Fernando Zambrano Stelia Carolina Méndez-Sanchez Fernando Rodríguez-Sanabria Elena E. Stashenko Jonny E. Duque Luna | 2014 | Parasitology Research2014，，7： | 1 |
| 18 | Th1 immune response promotes severe bone resorption caused by porphyromonas gingivalis显示文摘 | Stashenko P Goncalves RB Lipkin B | 2007 | Am J Pathol2007，170，1： | 1 |
| 19 | Serotherapy of a patientwith a monoclonal antibody directed against a human lymphoma-associated antigen显示文摘 | Nadler LM Stashenko P Hardy R | 1980 | Cancer Res1980，40，9： | 1 |
| 20 | Monitoring volatile chemicals formed from must during yeast fermentation 显示文摘 | Stashenko H Macku C Shibamato T | 1992 | Agric Food Chem1992，40，： | 1 |
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