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| 1 | A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice显示文摘瞄准:表示 23-ku pE2 到花费有效地,肝炎 E 病毒(HEV ) 的 3'-portion 包括的 E2 碎片编码的最有希望的子单元疫苗打开读物框架 2 (ORF2 ) 在烟草的质体(Nicotiana 烟 cv。SR1 ) ,在质体调查 transgene 表示和 pE2 累积,并且评估反在老鼠的导出质体的 pE2 的遗传因子的效果。方法:包含米饭 psbA 倡导者驾驶的 E2 碎片的指向质体的向量 pRB94-E2 被构造。在进烟草质体的交货之上,这构造能在质体染色体在 psaB-trnfM 和 trnG-psbC 碎片开始相应再结合,并且导致在二碎片之间插入的 transgene。pRB94-E2 与一个 biolistic 粒子轰炸方法被交付,并且转变质体的工厂被获得在补充 spectinomycin 的媒介上跟随炮轰的叶纸巾的新生。改革工厂的 Transplastomic 地位被 PCR 和南部的污点分析证实, transgene 表示被北污点分析调查,并且 pE2 的累积被 ELISA 测量。而且,蛋白浸出物被用来使老鼠免疫,并且在使免疫的老鼠的浆液样品的 pE2 反应的抗体的存在被 ELISA 学习。结果:PCR 和南部的污点分析证实的 Transplastomic 线能活跃地抄录 E2 mRNA。pE2 多肽象 13.27 microg/g 一样高被积累到水平新鲜叶子。pE2 能刺激使免疫的老鼠产生 pE2 特定的抗体。结论:HEV-E2 碎片能被插入到质体染色体,导出的 recombinant pE2 抗原是在老鼠遗传因子的反。因此,质体可以是为 HEV 疫苗的划算的生产的新奇来源。 | Yuan-Xiang Zhou Maggie Yuk-Ting Lee James Ming-Him Ng Mee-Len Chye Wing-Kin Yip Sze-Yong Zee Eric Lam | 2006 | World Journal of Gastroenterology2006，12，2： | 10 |
| 2 | 东西方酸相关性疾病的未来显示文摘 | Guido Tytgat Shiu-Kum Lam James W.Freston Jose D.Sollano 戎兰 陆玮 | 2000 | 中华消化杂志2000，20，5： | 6 |
| 3 | Profiles and removal efficiency of polycyclic aromatic hydrocarbons by two different types of sewage treatment plants in Hong Kong显示文摘Sewage discharge could be a major source of polycyclic aromatic hydrocarbons(PAHs) in the coastal waters. Stonecutters Island and Shatin Sewage Treatment Works(SCISTW and STSTW)in Hong Kong, adopted chemically enhanced primary treatment and biological treatment,respectively. This study aimed at(1) determining the removal efficiencies of PAHs,(2) comparing the capabilities in removing PAHs, and(3) characterizing the profile of each individual PAHs, in the two sewage treatment plants(STPs). Quantification of 16 PAHs was conducted by a Gas Chromatography. The concentrations of total PAHs decreased gradually along the treatment processes(from 301 ± 255 and 307 ± 217 ng/L to 14.9 ± 12.1 and 63.3 ± 54.1 ng/L in STSTW and SCISTW, respectively). It was noted that STSTW was more capable in removing total PAHs than SCISTW with average total removal efficiency 94.4% ± 4.12% vs. 79.2% ± 7.48%(p < 0.05). The removal of PAHs was probably due to sorption in particular matter, confirmed by the higher distribution coefficient of individual and total PAHs in solid samples(dewatered sludge contained92.5% and 74.7% of total PAHs in SCISTW and STSTW, respectively) than liquid samples(final effluent-total contained 7.53% and 25.3% of total PAHs in STSTW and SCISTW, respectively).Despite the impressive capability of STSTW and SCISTW in removing PAHs, there was still a considerable amount of total PAHs(1.85 and 39.3 kg/year, respectively for the two STPs) being discharged into Hong Kong coastal waters, which would be an environmental concern. | Yu Bon Man Ka Lai Chow Zhang Cheng Wing Yin Mo Yung Hau Chan James Chung Wah Lam Frankie Tat Kwong Lau Wing Cheong Fung Ming Hung Wong | 2017 | Journal of Environmental Sciences2017，29，3： | 5 |
| 4 | Entecavir Monotherapy Is Effective in Suppressing Hepatitis B Virus After Liver Transplantation显示文摘 | James Fung Cindy Cheung See–Ching Chan Man–Fung Yuen Kenneth S.H. Chok William Sharr Wing–Chiu Dai Albert C.Y. Chan Tan–To Cheung Simon Tsang Banny Lam Ching–Lung Lai Chung–Mau Lo | 2011 | Gastroenterology2011，，4： | 3 |
| 5 | Double-blind randomized sham controlled trial of intraperitoneal bupivacaine during emergency laparoscopic cholecystectomy显示文摘BACKGROUND: Intraperitoneal local anesthesia (IPLA) during elective laparoscopic cholecystectomy (el-LC) decreases post-operative pain. None of the studies have explored the efficacy of IPLA at emergency laparoscopic cholecystectomy (em-LC). A longer operative duration, the greater frequency of washing, and the inflammation associated with cholecystitis or pancreatitis are a few reasons why it cannot be assumed that a benefit in pain scores will be seen in em-LC with IPLA. This study was undertaken to assess the efficacy of IPLA in patients undergoing em-LC. METHODS: Double-blind randomized sham controlled trial was conducted of 41 consecutive subjects undergoing em-LC. IPLA was delivered by a combination of injection to the diaphragmatic and topical wash over the liver and gallbladder with bupivacaine or saline. The primary outcome was visual analogue scale pain scores until discharge. Secondary outcomes included pain scores in theatre recovery and analgesic consumption. RESULTS: One patient had a procedure converted to open and was excluded. There was no significant difference in pain scores in the ward or theatre recovery. Analgesic use, respiratory rate, oxygen saturation, duration to ambulation, eating, satisfaction scores, and time to discharge were comparable between the two groups. CONCLUSIONS: IPLA during em-LC does not influence postoperative pain. Other modalities of analgesia should be explored for decreasing the interval between diagnosis of acute admission and em-LC. | Keith J Roberts Jeff Gilmour Ruplay Pande James Hodson For Tai Lam Saboor Khan | 2013 | Hepatobiliary & Pancreatic Diseases International2013，12，3： | 3 |
| 6 | Phosphine oxide-functionalized polyfluorene derivatives:Synthesis,photophysics,electrochemical properties,and electroluminescence performance显示文摘A series of phosphine oxide-functionalized polyfluorene derivatives,PFH-PO-40-1 (P1),PFH-PO-20-1 (P2),PFH-PO-10-1 (P3),and PFH-PO-1-1 (P4),were prepared via a palladium-mediated Suzuki cross-coupling reaction.The structures and purities of all polymers were fully characterized by 1H and 13C NMR,UV-vis and photoluminescent spectroscopy,gel permeation chromatography,and TGA/DSC.Their emission features showed single broad peaks at about 445 nm in film,compared with those in dilute solutions,which might be caused by some degree of aggregation in the excited states of the backbones.The best electroluminescence (EL) performance of these polymers with configuration of ITO/PEDOT:PSS/Polymer/Alq3/LiF/Al was obtained from P1 (current efficiency was 4.2 Cd/A at 6V). | LIU DeAng GIBSON Gary BRUG James LAM Sity MAO Samuel S | 2011 | Science China Chemistry2011，54，4： | 3 |
| 7 | Health-related quality of life, work productivity, and health care resource utilization of subjects with irritable bowel syndrome: Baseline results from logic (longitudinal outcomes study of gastrointestinal symptoms in Canada), a naturalistic study显示文摘 | Pierre Paré James Gray Sy Lam Robert Balshaw Shideh Khorasheh Martin Barbeau Suzanne Kelly Christopher R. McBurney | 2006 | Clinical Therapeutics2006，，10： | 2 |
| 8 | Macrophage-specific inhibition of the histone demethylase JMJD3 decreases STING and pathologic inflammation in diabetic wound repair显示文摘Macrophage plasticity is critical for normal tissue repair following injury.In pathologic states such as diabetes,macrophage plasticity is impaired,and macrophages remain in a persistent proinflammatory state;however,the reasons for this are unknown.Here,using single-cell RNA sequencing of human diabetic wounds,we identified increased JMJD3 in diabetic wound macrophages,resulting in increased inflammatory gene expression.Mechanistically,we report that in wound healing,JMJD3 directs early macrophage-mediated inflammation via JAK1,3/STAT3 signaling.However,in the diabetic state,we found that IL-6,a cytokine increased in diabetic wound tissue at later time points post-injury,regulates JMJD3 expression in diabetic wound macrophages via the JAK1,3/STAT3 pathway and that this late increase in JMJD3 induces NFκB-mediated inflammatory gene transcription in wound macrophages via an H3K27me3 mechanism.Interestingly,RNA sequencing of wound macrophages isolated from mice with JMJD3-deficient myeloid cells(Jmjd3f/fLyz2Cre+)identified that the STING gene(Tmem173)is regulated by JMJD3 in wound macrophages.STING limits inflammatory cytokine production by wound macrophages during healing.However,in diabetic mice,its role changes to limit wound repair and enhance inflammation.This finding is important since STING is associated with chronic inflammation,and we found STING to be elevated in human and murine diabetic wound macrophages at late time points.Finally,we demonstrate that macrophage-specific,nanoparticle inhibition of JMJD3 in diabetic wounds significantly improves diabetic wound repair by decreasing inflammatory cytokines and STING.Taken together,this work highlights the central role of JMJD3 in tissue repair and identifies cell-specific targeting as a viable therapeutic strategy for nonhealing diabetic wounds. | Christopher O.Audu William J.Melvin Amrita D.Joshi Sonya J.Wolf Jadie Y.Moon Frank M.Davis Emily C.Barrett Kevin D.Mangum Hongping Deng Xianying Xing Rachel Wasikowski Lam C.Tsoi Sriganesh B.Sharma Tyler M.Bauer James Shadiow Matthew A.Corriere Andrea TObi Steven LKunkel Benjamin Levi Bethany BMoore Johann EGudjonsson Andrew MSmith Katherine A.Gallagher | 2022 | Cellular & Molecular Immunology2022，19，11： | 2 |
| 9 | Identification of Adropin as a Secreted Factor Linking Dietary Macronutrient Intake with Energy Homeostasis and Lipid Metabolism显示文摘 | K. Ganesh Kumar James L. Trevaskis Daniel D. Lam Gregory M. Sutton Robert A. Koza Vladimir N. Chouljenko Konstantin G. Kousoulas Pamela M. Rogers Robert A. Kesterson Marie Thearle Anthony W. Ferrante Randall L. Mynatt Thomas P. Burris Jesse Z. Dong Heathe | 2008 | Cell Metabolism2008，，： | 2 |
| 10 | A new delay system approach to networked - based control 显示文摘 | GAO Huijun CHEN Tongwen Lam James | 2008 | Auto- matic2008，44，1： | 1 |
| 11 | Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation显示文摘 | LAM C W JAMES JT Mc CLUSKEY R | 2004 | Toxicol Sci2004，77，1： | 1 |
| 12 | Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation显示文摘 | Lam CW James JT McCluskey R | 2004 | Toxicol Sci2004，77，1： | 1 |
| 13 | Robust stabilization for uncertain discrete singular systems显示文摘 | Xu S Y Cheng W Y Yu G N James Lam | 2001 | Automatica2001，37，5： | 1 |
| 14 | Robust Stabilization of Delayed Singular Systems with Linear Fractional Parametric Uncertainties显示文摘 | Shaosheng Zhou James Lam | 2003 | Circuits Systems & Signal Processing2003，，6： | 1 |
| 15 | Pulmonary toxicity of single wall carbon nano-tubos in mice 7 and 90 days after intratracheal instillation显示文摘 | LAM C W JAMES J T MCCLUSKEY R | 2004 | Toxicological Sciences2004，77，1： | 1 |
| 16 | Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation显示文摘 | Lam CW James JT Mccluskey R | 2004 | Toxicol Sci2004，77，1： | 1 |
| 17 | Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation显示文摘 | Lam C W James J T McCluskey R | 2004 | Toxicological Sciences2004，77，： | 1 |
| 18 | pulmonary toxicity of single-well carbon nanotubes in mice 7 and 90 days after intratracheal instillation显示文摘 | LAM C W JAMES J T MCCLUSKEY R | 2004 | Toxicological Sciences2004，77，1： | 1 |
| 19 | On positive realness of descriptor system显示文摘 | James Lam Xu S Y | 2000 | IEEE Trans on Circuits and Systems-I: Fundamental Theory and Applications2000，49，3： | 1 |
| 20 | Pulmonary toxicity of single-wall carbon nanotubes in Mice 7 and 90 days after intratracheal instillation显示文摘 | Chiu-Wing Lam James J T Richard McCluskey | 2004 | Toxicological Sciences2004，77，1： | 1 |
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