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| 1 | Grade classification of neuroepithelial tumors using high-resolution magic-angle spinning proton nuclear magnetic resonance spectroscopy and pattern recognition显示文摘Clinical data have shown that survival rates vary considerably among brain tumor patients,according to the type and grade of the tumor.Metabolite profiles of intact tumor tissues measured with high-resolution magic-angle spinning proton nuclear magnetic resonance spectroscopy (HRMAS 1H NMRS) can provide important information on tumor biology and metabolism.These metabolic fingerprints can then be used for tumor classification and grading,with great potential value for tumor diagnosis.We studied the metabolic characteristics of 30 neuroepithelial tumor biopsies,including two astrocytomas (grade I),12 astrocytomas (grade II),eight anaplastic astrocytomas (grade III),three glioblastomas (grade IV) and five medulloblastomas (grade IV) from 30 patients using HRMAS 1H NMRS.The results were correlated with pathological features using multivariate data analysis,including principal component analysis (PCA).There were significant differences in the levels of N-acetyl-aspartate (NAA),creatine,myo-inositol,glycine and lactate between tumors of different grades (P<0.05).There were also significant differences in the ratios of NAA/creatine,lactate/creatine,myo-inositol/creatine,glycine/creatine,scyllo-inositol/creatine and alanine/creatine (P<0.05).A soft independent modeling of class analogy model produced a predictive accuracy of 87% for high-grade (grade III-IV) brain tumors with a sensitivity of 87% and a specificity of 93%.HRMAS 1H NMR spectroscopy in conjunction with pattern recognition thus provides a potentially useful tool for the rapid and accurate classification of human brain tumor grades. | CHEN WenXue LOU HaiYan ZHANG HongPing NIE Xiu LAN WenXian YANG YongXia XIANG Yun QI JianPin LEI Hao TANG HuiRu CHEN FenEr DENG Feng | 2011 | Science China(Life Sciences)2011，54，7： | 5 |
| 2 | Lithofacies palaeogeography as a guide to petroleum exploration显示文摘Lithofacies palaeogeography as a guide to petroleum exploration is a very important topic.By using the following five exploration examples,this paper discusses the guide of lithofacies palaeogeography or of sedimentary facies to petroleum exploration.These examples include the dolostone of the Lower Ordovician Majiagou Formation 5 in the Ordos area,the Donghe Sandstone of the Upper Devonian and Lower Carboniferous in the Tarim Basin,the reef of the Upper Permian Changxing Formation in the Sichuan Basin,the oolitic bank of the Lower Triassic Feixianguan Formation in the Sichuan Basin,and the lacustrine delta sediments and gravity flow sediments of the Middle and Upper Triassic Yanchang Formation in the Ordos Basin. | Feng Zengzhao Bao Hongping Jia Jinhua Wang Yigang Deng Xiuqin | 2013 | Journal of Palaeogeography2013，2，2： | 3 |
| 3 | Extensive evolution analysis of the global chikungunya virus strains revealed the origination of CHIKV epidemics in Pakistan in 2016显示文摘Chikungunya virus(CHIKV) is a mosquito-borne virus that causes epidemics widely in the world especially in the tropical and subtropical regions. Phylogenetic analysis has found that the CHIKV lineages were associated with the spatial and temporal distributions, which were related to the virus adaption to the major mosquito species and their distributions. In this study, we reported the complete genome sequences of eight CHIKV isolates from the outbreak in Pakistan last year. Then we reviewed the evolutionary history using extensive phylogenetic analysis, analyzed lineagespecific substitutions in viral proteins, and characterized the spreading pathway of CHIKV strains including the Pakistani strains. The results showed that the Pakistani stains belonged to the ECSA.IOL sub-lineage and derived from India. The genetic properties of the Pakistani strains including the adaptive substitution to vectors were further characterized, and the potential risks from the occurrence of CHIKV infection in Pakistan were discussed. These results provided better understanding of CHIKV evolution and transmission in the world and revealed the possible origination of the CHIKV outbreak and epidemic in Pakistan, which would promote the disease prevention and control in the identified countries and territories with the history of CHIKV infections as well as new regions with potential risk of CHIKV outbreaks. | Junming Shi Zhengyuan Su Zhaojun Fan Jun Wang Siqing Liu Bo Zhang Hongping Wei Shoukat Jehan Nadia Jamil Shu Shen Fei Deng | 2017 | Virologica Sinica2017，32，6： | 2 |
| 4 | Macrophage-specific inhibition of the histone demethylase JMJD3 decreases STING and pathologic inflammation in diabetic wound repair显示文摘Macrophage plasticity is critical for normal tissue repair following injury.In pathologic states such as diabetes,macrophage plasticity is impaired,and macrophages remain in a persistent proinflammatory state;however,the reasons for this are unknown.Here,using single-cell RNA sequencing of human diabetic wounds,we identified increased JMJD3 in diabetic wound macrophages,resulting in increased inflammatory gene expression.Mechanistically,we report that in wound healing,JMJD3 directs early macrophage-mediated inflammation via JAK1,3/STAT3 signaling.However,in the diabetic state,we found that IL-6,a cytokine increased in diabetic wound tissue at later time points post-injury,regulates JMJD3 expression in diabetic wound macrophages via the JAK1,3/STAT3 pathway and that this late increase in JMJD3 induces NFκB-mediated inflammatory gene transcription in wound macrophages via an H3K27me3 mechanism.Interestingly,RNA sequencing of wound macrophages isolated from mice with JMJD3-deficient myeloid cells(Jmjd3f/fLyz2Cre+)identified that the STING gene(Tmem173)is regulated by JMJD3 in wound macrophages.STING limits inflammatory cytokine production by wound macrophages during healing.However,in diabetic mice,its role changes to limit wound repair and enhance inflammation.This finding is important since STING is associated with chronic inflammation,and we found STING to be elevated in human and murine diabetic wound macrophages at late time points.Finally,we demonstrate that macrophage-specific,nanoparticle inhibition of JMJD3 in diabetic wounds significantly improves diabetic wound repair by decreasing inflammatory cytokines and STING.Taken together,this work highlights the central role of JMJD3 in tissue repair and identifies cell-specific targeting as a viable therapeutic strategy for nonhealing diabetic wounds. | Christopher O.Audu William J.Melvin Amrita D.Joshi Sonya J.Wolf Jadie Y.Moon Frank M.Davis Emily C.Barrett Kevin D.Mangum Hongping Deng Xianying Xing Rachel Wasikowski Lam C.Tsoi Sriganesh B.Sharma Tyler M.Bauer James Shadiow Matthew A.Corriere Andrea TObi Steven LKunkel Benjamin Levi Bethany BMoore Johann EGudjonsson Andrew MSmith Katherine A.Gallagher | 2022 | Cellular & Molecular Immunology2022，19，11： | 2 |
| 5 | Efficient production of pronuclear embryos in breeding and nonbreeding season for generating transgenic sheep overexpressing TLR4显示文摘Background: Brucella is a zoonotic Gram-negative pathogen that causes abortion and infertility in ruminants and humans. TLR4 is the receptor for LPS which can recognize Brucella and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. Consequently, transgenic sheep over-expressing TLR4 are an suitable model to investigate the effects of TLR4 on preventing Brucellosis. In this study, we generated transgenic sheep overexpressing TLR4 and aimed to evaluate the effects of different seasons(breeding and non-breeding season) on superovulation and the imported exogenous gene on growth.Results: In total of 43 donor ewes and 166 recipient ewes in breeding season, 37 donor ewes and 144 recipient ewes in non-breeding season were selected for super-ovulation and injected embryo transfer to generate transgenic sheep.Our results indicated the no. of embryos recovered of donors and the rate of pronuclear embryos did not show any significant difference between breeding and non-breeding seasons(P > 0.05). The positive rate of exogenous TLR4 tested were 21.21 % and 22.58 % in breeding and non-breeding season by Southern blot. The expression level of TLR4 in the transgenic sheep was 1.5 times higher than in the non-transgenic group(P < 0.05). The lambs overexpressing TLR4 had similar growth performance with non-transgenic lambs, and the blood physiological parameters of transgenic and non-transgenic were both in the normal range and did not show any difference.Conclusions: Here we establish an efficient platform for the production of transgenic sheep by the microinjection of pronuclear embryos during the whole year. The over-expression of TLR4 had no adverse effect on the growth of the sheep. | Yan Li Di Lian Shoulong Deng Xiaosheng Zhang Jinlong Zhang Wenting Li Hai Bai Zhixian Wang Hongping Wu Juncai Fu Hongbing Han Jianzhong Feng Guoshi Liu Ling Lian Zhengxing Lian | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 2 |
| 6 | Existence of Separate Domains in Lysin PlyG for Recognizing Bacillus anthracis Spores and Vegetative Cells显示文摘 | Hang Yang Dian-Bing Wang Qiuhua Dong Zhiping Zhang Zongqiang Cui Jiaoyu Deng Junping Yu Xian-en Zhang Hongping Wei | 2012 | Antimicrobial Agents and Chemotherapy2012，，10： | 1 |
| 7 | Characterization of organic phases in the interlayer of montmorillonite using FTIR and 13 C NMR显示文摘 | Jianxi Zhu Hongping He Lizhong Zhu Xiaoyan Wen Feng Deng | 2005 | Journal of Colloid And Interface Science2005，，1： | 1 |
| 8 | Synthesis and luminescence properties of glass ceramics containing MSiO 3 :Eu 2+ (M=Ca, Sr, Ba) phosphors for white LED显示文摘 | Zhiguang Cui Guohua Jia Degang Deng Youjie Hua Shilong Zhao Lihui Huang Huanping Wang Hongping Ma Shiqing Xu | 2011 | Journal of Luminescence2011，，1： | 1 |
| 9 | Tracing drug release process with dual-modal hyperbranched polymer-gold nanoparticle complexes显示文摘Dual-modal surface enhanced Raman spectrum(SERS)-fluorescence polymer/metal hybrid complexes have been prepared for tracing drug release process in tumor cells. Firstly, the hyperbranched poly((S-(4-vinyl) benzyl S′-propyltrithiocarbonate)-co-(poly(ethylene glycol) methacrylate))(HPVBEG) was synthesized via the combination of reversible addition-fragmentation chain-transfer(RAFT) polymerization and self-condensing vinyl polymerization(SCVP). Subsequently, the anticancer drug doxorubicin(DOX) was linked to HPVBEG via pH sensitive Schiff base bonds to form HPVBEG-g-DOX conjugates.Through aminolysis reaction, HPVBEG-g-DOX was coordinated with gold nanoparticles(GNP), resulting in the formation of HPVBEG-g-DOX/GNP complexes. In neutral condition, the HPVBEG-g-DOX/GNP complexes were stable, and DOX was bound to the surface of GNPs. Therefore, the SERS of DOX could be observed, while the fluorescence of DOX was quenched by GNPs. Under an acidic environment, DOX was released from the surface of GNPs with breakage of Schiff base bonds.Thus, the SERS signal of DOX was gradually reduced. Correspondingly, the fluorescence signal of DOX was enhanced.Through dual-modal SERS-fluorescence technique, the DOX delivery and release process was traced in tumor cells. Moreover,the viability of MCF-7 cells incubated with HPVBEG-g-DOX/GNP complexes was investigated by Cell Counting Kit-8(CCK-8) assay. The experimental results showed that HPVBEG-g-DOX/GNP complexes had similar proliferation inhibition effect compared with free DOX. Definitely, the dual-modal SERS-fluorescence complexes for tracing drug delivery and release will have promising prospects on tumor diagnosis and therapy. | Yuanyuan Zhuang Dali Wang Chunhui Yin Hongping Deng Mo Sun Lin He Yue Su Xinyuan Zhu | 2016 | Science China Chemistry2016，59，12： | 0 |
| 10 | The core-merging giant impact in Earth's accretion history and its implications显示文摘The Earth’s accretion process is accompanied by a large number of collisions.It is widely accepted that collisions dominate the Earth’s late accretion stage.Among all these collisions,there is a special type of collision called Core-merging giant impact(CMGI),in which much or most the impactor’s core merges directly with the protoEarth’s core.This core-merging scenario plays an important role in the Earth’s accretion process and deeply affects the formation of the Earth’s core and mantle.However,because CMGI is a small probability event,it has not been fully studied.Here we use the SPH method to comprehensively study all possible CMGIs in the Earth’s accretion history.We find that CMGI only occurs in the initial conditions with small impact angle,small impact velocity and big impactor.We further discuss the implications of CMGI.We are confident that CMGI inevitably causes the chemical disequilibrium of the Earth’s core and mantle.The CMGI process also brings many light elements into the Earth’s core.In particular,if the Moon-forming giant impact is a CMGI,then CMGI can also explain the abnormal content of HSEs in the Earth’s current mantle. | You Zhou Yun Liu Christian Reinhardt Hongping Deng | 2022 | Acta Geochimica2022，41，4： | 0 |
| 11 | Detection, isolation, and characterization of chikungunya viruses associated with the Pakistan outbreak of 2016–2017显示文摘The chikungunya virus(CHIKV) is a mosquito-transmitted alphavirus, which has infected millions of people in Africa, Asia, Americas, and Europe since it reemerged in India and Indian Ocean regions in 2005–2006. Starting in the middle of November 2016, CHIKV has been widely spread, and more than 4,000 cases of infections in humans were confirmed in Pakistan. Here, we report the first isolation and characterization of CHIKV from the Pakistan outbreak. Eight CHIKV strains were newly isolated from human serum samples using a cell culture procedure. A full-length genome sequence and eight complete envelope(E1) sequences of CHIKV from Pakistan were obtained in this study. Alignment of the CHIKV E1 sequences revealed that the eight new CHIKV isolates were highly homogeneous, with only two nonsynonymous substitutions found at generally conserved sites(E99 and Q235). Based on the comparison of 342 E1 sequences, the two nonsynonymous mutations were located in well-recognized domains associated with viral functions such as the cell fusion and vector specificity, suggesting their potential functional importance. Phylogenetic analysis indicated that the CHIKV strains from Pakistan originated from CHIKV circulating in the Indian region. This study helps elucidate the epidemics of CHIKV in Pakistan and also provides a foundation for studies of evolution and expansion of CHIKV in South Asia. | Si-Qinq Liu Xiao Li Ya-Nan Zhang Ai-Li Gao Cheng-Lin Deng Jun-Hua Li Shoukat Jehan Nadia Jamil Fei Deng Hongping Wei Bo Zhang | 2017 | Virologica Sinica2017，32，6： | 0 |
| 12 | The ET mission to search for earth 2.0s显示文摘“Are we alone in the universe?”This fundamental question is as old as humankind itself.Since the dawn of civilization,humanity has wonderedwhether there is life elsewhere in the universe and,if so,whether it is hosted on a second Earth.The discovery of the first extrasolar planet(also called an exoplanet),51 Pegasi b,around a sun-like star in 19951 dramatically changed our understanding of the extrasolar planet worlds and challenged the uniqueness of our solar system.Mayor and Queloz were awarded the Nobel Prize in physics in 2019 for thismajor achievement. | Jian Ge Hui Zhang Hongping Deng Steve BHowell the ET team | 2022 | The Innovation2022，3，4： | 0 |
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