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| 1 | O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an “O-Follow-N” rule显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2),the causative agent of coronavirus disease 2019(COVID-19),emerged in late 2019 and has since caused a pandemic.Although there have been extensive studies worldwide,our understanding of this newly emerged pathogen is far from sufficient.The pathogenesis of the SARS-CoV-2 infection is not fully understood,although a“two-stage”hypothesis was proposed in our previous study. | Wenmin Tian Delin Li Nan Zhang Guijie Bai Kai Yuan Haixia Xiao Feng Gao Yang Chen Catherine C.L.Wong George Fu Gao | 2021 | Cell Research2021，31，10： | 9 |
| 2 | Combined prednisone and levothyroxine improve treatment of severe thrombocytopenia in hepatitis B with compensatory cirrhosis accompanied by subclinical and overt hypothyroidism显示文摘The aim of the present study was to investigate the relationship between hypothyroidism and thrombocytopenia in hepatitis Brelated compensatory liver cirrhosis and to determine whether treatment with levothyroxine and prednisone is superior in a multicenter, open-label, observational study in China. In total, 125 consecutive hepatitis B-related compensated liver cirrhosis patients with severe thrombocytopenia accompanied by hypothyroidism were included. The patients were divided into four groups according to treatment strategy: a control group(n=29), a prednisone group(n=25), a levothyroxine group(n=32) and a prednisone plus levothyroxine group(n=39). Severe thrombocytopenia was more prevalent in hepatitis B-associated compensatory liver cirrhosis patients with hypothyroidism than in euthyroid patients(29.6% vs. 14.7%, P<0.05). Combination treatment with prednisone and levothyroxine decreased the risk of bleeding and improved platelet recovery compared to control treatment and treatment with either prednisone or levothyroxine alone. The platelet count before therapy, serum thyroid stimulating hormone and combination treatment with prednisone and levothyroxine were associated with bleeding events. Therefore, the present study suggests that hypothyroidism is associated with severe thrombocytopenia in hepatitis B-associated compensatory liver cirrhosis. Treatment with prednisone plus levothyroxine may present a novel approach in these patients. | Jing Xue Ru Feng Haixia Fu Qian Jiang Hao Jiang Jing Lu Hui Liu Jingwen Wang Ting Niu Xin Wang YANDi Xie Hao Wang Lanping Xu Kaiyan Liu Xiaojun Huang Xiaohui Zhang | 2018 | Science China(Life Sciences)2018，61，8： | 8 |
| 3 | Arsenic trioxide alleviates acute graft-versus-host disease by modulating macrophage polarization显示文摘This study aimed to explore macrophage polarization in acute graft-versus-host disease after hematopoietic stem cell transplantation, and investigated if arsenic trioxide(ATO) could correct this imbalance. In the colon of GVHD mice, we found that the number of F4/80+iNOS+ cells as well as the expression intensity of TNF-α and IL-1β was greater in the GVHD group than in the BM group, whereas the number of F4/80+CD206+ cells and the expression intensity of IL-10 and TGF-β was greater in the BM group than in the GVHD group. We investigated the effect of ATO on GVHD mice, and found that ATO treatment clearly improved the survival of the mice and reduced the severity of GVHD. In addition, ATO reduced the number of F4/80+iNOS+ cells, and increased the number of F4/80+CD206+ cells in the colon of GVHD mice. Furthermore, ATO sharply decreased CD86 and CD80 expression, and increased CD163 and CD206 expression in macrophages induced from aGVHD patients. Therefore,ATO can modulate the M1 and M2 phenotype in GVHD mice or in macrophages from aGVHD patients. Our data suggest that macrophage polarization is involved in the pathogenesis of aGVHD, and ATO treatment modulates macrophage polarization toward an M2 phenotype. | Xiao Liu Yan Su Xueyan Sun Haixia Fu Qiusha Huang Qi Chen Xiaodong Mo Meng Lv Yuan Kong Lanping Xu Xiaojun Huang Xiaohui Zhang | 2020 | Science China(Life Sciences)2020，63，11： | 6 |
| 4 | Gut microbiome alterations and its link to corticosteroid resistance in immune thrombocytopenia显示文摘Quantitative metagenomic studies have linked the gut microbiota to autoimmune disorders.Here,we performed deep shotgun metagenomic sequencing of fecal samples from 99 immune thrombocytopenia(ITP)patients and 52 healthy controls.Dysbiosis in the gut microbiome of ITP was detected phylogenetically and functionally,and classifier based on species markers distinguished individuals with ITP from healthy controls.In particular,the abundance of Ruminococcus gnavus,Bifidobacterium longum and Akkermansia muciniphila was markedly increased in treatment-na?ve ITP patients,and the alterations of microbial species were correlated with clinical indices.Functionally,the secondary bile acid biosynthesis and flagellar assembly were depleted in the gut microbiota of ITP,which may contribute to the onset of ITP by affecting the immune system.Furthermore,we found that corticosteroid treatment affected the gut microbiome of ITP.Compared with corticosteroid-sensitive ITP patients,we identified that the corticosteroid-resistant ITP patients displayed a distinct gut microbiome,which was different from that of the treatment-na?ve ITP patients.Together,we provided support for the critical role of gut microbiota in the development of ITP and established a foundation for further research characterizing gut microbiota in relation to corticosteroid resistance of ITP. | Yanan Wang Fengqi Liu Gaochao Zhang Yan Su Xueyan Sun Qi Chen Chencong Wang Haixia Fu Yun He Xiaolu Zhu Xiao Liu Meng Lv Xiangyu Zhao Xiaosu Zhao Yueying Li Qianfei Wang Xiaojun Huang Xiaohui Zhang | 2021 | Science China(Life Sciences)2021，64，5： | 6 |
| 5 | Reduced β2-GPI is associated with increased platelet aggregation and activation in patients with prolonged isolated thrombocytopenia after allo-HSCT显示文摘We aimed to measure platelet function and its relationship with β2-GPI in prolonged isolated thrombocytopenia(PT) after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Fifty-six patients with PT and 60 allo-HSCT recipients without PT(non-PT controls) were enrolled.Platelet aggregation and activation,β2-GPI and anti-β2-GPI antibody levels,vWF antigen,and vWF activity were analyzed.The effect of β2-GPI on platelet aggregation was also measured ex vivo.Results showed that ADP-induced platelet aggregation significantly increased(39%±7.5% vs.23%±8.5%,P=0.032),and the platelet expression of both CD62 p(33.6%±11.6% vs.8.5%±3.5%,P<0.001) and PAC-1(42.4%±7.6% vs.6.8%±2.2%,P<0.001) was significantly higher in patients with PT than in those without PT.Significantly lower β2-GPI levels(164.2±12 μg m L^-1 vs.234.2±16 μg mL^-1,P<0.001),higher anti-β2-GPI IgG levels(1.78±0.46 U mL^-1 vs.0.94±0.39 U mL^-1,P<0.001),and increased vWF activity(133.06%±30.50% vs.102.17%±25.90%,P<0.001) were observed in patients with PT than in those without PT.Both ADPinduced platelet aggregation(n=116,r^2=-0.5042,P<0.001) and vWF activity(n=116,r^2=-0.2872,P<0.001) were negatively correlated with β2-GPI levels.In summary,our data suggested that platelet aggregation and activation were significantly higher in patients with PT than in those without PT,which might be associated with reduced β2-GPI levels.The reduced β2-GPI levels might be due to the existence of anti-β2-GPI IgG. | Haixia Fu Jingzhong Zhao Lanping Xu Kaiyan Liu Yu Wang Huan Chen Wei Han Jingzhi Wang Fengrong Wang Xiaojun Huang Xiaohui Zhang | 2019 | Science China(Life Sciences)2019，62，7： | 4 |
| 6 | Pulse radiolysis study on gatifloxacin——A fluoroquinolone antibiotic显示文摘The reactions between gatifloxacin(GFX) and various one-electron oxidants,such as ˙OH,N3˙,Br2˙ˉ,and SO4˙ˉ,have been studied by pulse radiolysis techniques.The GFX radical anion formed in the reaction of GFX with eaqˉ could either be protonated or deprotonated,and the absorption of GFX radical anion was located at 390 nm.The transient species produced by the reaction of GFX with ˙OH radical shows a broad band in the 380?600 nm region with a shoulder,while the oxidation by N3˙,SO4˙ˉ,and Br2˙ˉ results in an absorption band with λmax = 370 nm.At neutral condition(pH 7),the rate constants of GFX reacting with ˙OH,N3˙,Br2˙ˉ,SO4˙ˉ and eaqˉ are estimated to be 1.0 × 1010,3.1 × 109,2.8 × 109,3.0 × 109,and 1.8 × 1010 dm3 mol?1 s?1,respectively.From the pH dependence on the formation of electron adducts and on the rate constant of GFX with eaqˉ,the pKa of GFX radical anion is estimated to be 5.5 and 9.3. | LI HaiXia LIU YanCheng TANG RuiZhi ZHANG Peng FU HaiYing YAO SiDe WANG WenFeng | 2012 | Science China Chemistry2012，55，7： | 3 |
| 7 | Oncostatin M inhibits myoblast differentiation and regulates muscle regeneration显示文摘Oncostatin M (OSM ) 是 interleukin-6 家庭的 cytokine 并且在发炎期间起重要作用。然而,它在 myoblast 区别的角色和肌肉新生仍然保持未经勘探。我们这里证明 OSM potently 主要由激活 JAK1/STAT1/STAT3 小径禁止了 myoblast 区别。OSM downregulated myocyte 提高有约束力的因素 2A (MEF2A ) , upregulated Id1 和 Id2 的表示,并且禁止了 MyoD 和 MEF2 的 transcriptional 活动。另外, OSM 也提高了 STAT3 和 OSM 受体的表示,它组成了一个积极反馈环进一步放大导致 OSM 的发信号。而且,我们发现身体上与 MEF2 联系并且镇压的那 STAT1 它的 transcriptional 活动,它能说明 MEF2 的调停 OSM 的压抑。尽管在在 vivo 的正常肌肉无法发现, OSM 很快在肌肉损害上然后即时被导致就在 myoblasts 的多数前的 downregulated 区分。没有影响 myoblast 增长,在肌肉的 OSM 的延长表示损害了新生进程,建议 OSM 工作阻止增殖的 myoblasts 在肌肉新生的早阶段期间的早熟的区别。 | Fang Xiao Haixia Wang Xinrong Fu Yanfeng Li Kewei Ma Luguo Sun Xiang Gao Zhenguo Wu | 2011 | Cell Research2011，21，2： | 2 |
| 8 | 2021 Chinese consensus on the diagnosis and management of primary immune thrombocytopenia in pregnancy显示文摘Immune thrombocytopenia(ITP)is an acquired disease characterized by isolated thrombocytopenia,which is one of the most common causes of thrombocytopenia during pregnancy.Women with ITP who have severe thrombocytopenia are at an increased risk for life-threatening obstetric complications.Therefore,we established this consensus statement on the diagnosis and management of ITP during pregnancy(detailed information is available in the Supplementary File,http://gffzz16e312a87a7141a5hp9w5nc9vx5qx609w.ffgz.tsg.suse.edu.cn/A978). | Zhang Xiaohui Chen Fangping Chen Xiequn Cheng Yunfeng Fang Meiyun Feng Jianming Fu Haixia Gao Hong Han Yue He Aili Hou Ming Hu Yu Huang Ruibin Huang Wenrong Jing Zhicheng Kong Peiyan Liang Aibin Liang Meiying Liu Daihong Liu Junling Liu Lin Liu Xiaowei Ma Liangming Mei Heng Ni Heyu Niu Ting Peng Jun Qiao Jianlin Ren Jinhai Song Yongping Tang Liang V Tong Tong Wang Shaoyuan Wang Xin Wang Zhao Wei Hui Wu Depei Wu Guangsheng Xu Caigang Xu Xue Xu Yajing Yang Linhua Yang Renchi Yang Tonghua Yin Chenghong Yu Li Zhang Guangsen Zhang Lei Zhang Liansheng Zhang Xi Zhao Weili Zhao Yongqiang Zhou Daobin Zhou Hu Zhou Zeping Zhu Tienan Wang Jianliu Huang Xiaojun | 2022 | Chinese Medical Journal2022，，8： | 2 |
| 9 | Effects of ultrasonic treatment on the physicochemical properties and DPPH radical scavenging activity of polysaccharides from mushroom lnonotus obliquus显示文摘 | FU Lingling CHEN Haixia DONG Peng | 2010 | Journal of Food Science2010，75，4： | 1 |
| 10 | Physicochemical properties and antioxidant capacity of 3 polysaccharides from greentea,oolong tea,and black tea显示文摘 | CHEN Haixia QU Zhishuang FU Lingling | 2009 | Journal of Food Science2009，74，6： | 1 |
| 11 | Prolonged Thrombocytopenia Following Allogeneic Hematopoietic Stem Cell Transplantation and Its Association with a Reduction in Ploidy and an Immaturation of Megakaryocytes显示文摘 | Zhang Xiaohui Fu Haixia Xu Lanping | 2011 | Biol Blood Marrow Transplant2011，17，2： | 1 |
| 12 | Physicochemical characterization and antioxidant activity of a polysaccharide isolated from oolong tea显示文摘 | Haixia Chen Zhaoshuai Wang Zhishuang Qu Lingling Fu Peng Dong Xin Zhang | 2009 | European Food Research and Technology2009，，4： | 1 |
| 13 | Genome-wide high-resolution mapping of mitotic DNA synthesis sites and common fragile sites by direct sequencing显示文摘Common fragile sites(CFSs)are genomic loci prone to the formation of breaks or gaps on metaphase chromosomes.They are hotspots for chromosome rearrangements and structural variations,which have been extensively implicated in carcinogenesis,aging,and other pathological processes.Although many CFSs were identified decades ago,a consensus is still lacking for why they are particularly unstable and sensitive to replication perturbations.This is in part due to the lack of high-resolution mapping data for the vast majority of the CFSs,which has hindered mechanistic interrogations.Here,we seek to map human CFSs with high resolution on a genome-wide scale by sequencing the sites of mitotic DNA synthesis(MiDASeq)that are specific for CFSs.We generated a nucleotide-resolution atlas of MiDAS sites(MDSs)that covered most of the known CFSs,and comprehensively analyzed their sequence characteristics and genomic features.Our data on MDSs tallied well with long-standing hypotheses to explain CFS fragility while highlighting the contributions of late replication timing and large transcription units.Notably,the MDSs also encompassed most of the recurrent double-strand break clusters previously identified in mouse neural stem/progenitor cells,thus bridging evolutionarily conserved break points across species.Moreover,MiDAseq provides an important resource that can stimulate future research on CFSs to further unravel the mechanisms and biological relevance underlying these labile genomic regions. | Fang Ji Hongwei Liao Sheng Pan Liujian Ouyang Fang Jia Zaiyang Fu Fengjiao Zhang Xinwei Geng Xinming Wang Tingting Li Shuangying Liu Madiha Zahra Syeda Haixia Chen Wen Li Zhihua Chen Huahao Shen Songmin Ying | 2020 | Cell Research2020，30，11： | 1 |
| 14 | Increased acetylcholinesterase and capase-3 expression in the brain and peripheral immune system of focal cerebral ischemic rats显示文摘 | Tao Hu Qiang Fu Xiwei Liu Haixia Zhang Menghua Dong | 2009 | Journal of Neuroimmunology2009，，： | 1 |
| 15 | Effects of aerosolized ketamine on the level of nitric oxide and nitric oxide synthetase in the lung tissue of rat with asthma显示文摘Objective: To explore the effects of aerosolized ketamine on the level of nitric oxide and nitric oxide synthetase in the lung tissue in rat asthma model. Methods: Forty SD rats were randomly assigned to five groups: control group (group N), asthma model group (group A), two pretreated groups of different concentrations of ketamine (group K1, K2) and dexamethasone group(group D) with eight rats in each group.The rats in group A were sensitized by injection of ovalbumin (OA) together with aluminum hydroxide and bordetella pertussis as adjuvants. Two weeks after the sensitization,aerosolized OA was used to cause asthma. The rats in group K1 and K2 were sensitized with OA as group A , and then exposed to aerosol of ketamine ,with the concentration of 25 g/L and 50 g/L respectively.Before using aerosolized OA,the rats in group D were exposed to aerosol of 0.01% dexamethasone .The level of NO2-/NO3- in lung tissues, inducible nitric oxide synthetase(iNOS) and constitute nitric oxide synthetase(cNOS) was measured in all groups. Results: The level of NO2-/NO3- and the activity of iNOS in lung tissues in group A were signiticantly higher than those in the other groups. The iNOS activity and the level of NO2-/NO3- in lung tissues were highly positively correlated. Conclusion: NO can induce airway hyperreactivity that may worsen asthma. Aerosolized ketamine can decrease the iNOS expression and reduce the level of NO in the lung tissue in rat asthma model. | Weiqian Tian Chengzhang Fu Minmin Zhu Haixia Li | 2006 | Journal of Nanjing Medical University2006，20，2： | 0 |
| 16 | Rumen microbiota-host transcriptome interaction mediates the protective effects of trans-10,cis-12 CLA on facilitating weaning transition of lambs显示文摘Developing alternatives to antibiotics for prevention of gastrointestinal dysbiosis in early-weaning farmed animals is urgently needed.This study was to explore the potential effects of trans-10,cis-12 conjugated linoleic acid(CLA)on maintaining ruminal homeostasis of young ruminants during the weaning transition period.Thirty neonatal lambs were selected(6 lambs per group)and euthanized for rumen microbial and epithelial analysis.The lambs were weaned at 28 d and experienced the following 5 treatments:euthanized on d 28 as the pre-weaning control(CON0),fed starter feed for 5(CON5)or 21(CON21)d,fed starter feed with 1%of CLA supplemented for 5(CLA5)or 21(CLA21)d.Results showed that the average daily weight gain and dry matter intake were significantly higher in CLA5 than CON5 group.As compared with the CON5 and CON21 group,the relative abundances of volatile fatty acid(VFA)producing bacteria including Bacteroides,Treponema,Parabacteroides and Anaerovibrio,as well as the concentrations of acetate,butyrate and total VFA were significantly increased in CLA5 and CLA21 group,respectively.Integrating microbial profiling and epithelial transcriptome results showed that 7 downregulated inflammatory signaling-related host genes IL2RA,CXCL9,CD4,CCR4,LTB,SPP1,and BCL2A1 with CLA supplementation were significantly negatively correlated with both VFA concentration and VFA producing bacteria,while 3(GPX2,SLC27A2 and ALDH3A1)and 2(GSTM3 and GSTA1)upregulated metabolism-related genes,significantly positively correlated with either VFA concentration or VFA producing bacteria,respectively.To confirm the effects of CLA on epithelial signal transduction,in vitro experiment was further conducted by treating rumen epithelial cells without or with IL-17A+TNF-αfor 12 h after pretreatment of 100μM CLA or not(6 replicates per treatment).The results demonstrated the anti-inflammatory effect of CLA via suppressing the protein expression of NF-кB p-p65/p65 with the activation of peroxisome proliferator-activated receptor gamma(PPARγ).In conclusion,CLA supplementation enhanced the ruminal microbiota-driven transcriptional regulation in healthy rumen epithelial development via rumen VFA production,and CLA may therefore serve as an alternative way to alleviate early-weaning stress and improve physiological and metabolic conditions of young ruminants. | Chunlei Yang Xiangfei Deng Peter Lund Haixia Liu Xingwang Ding Zhengwei Fu Naifeng Zhang Jinjun Li Lifeng Dong | 2023 | Animal Nutrition2023，，1： | 0 |
| 17 | Xylan/chitosan composites prepared by an ionic liquid system with unique antioxidant properties显示文摘Antioxidant function and solubility in water are highly desirable in many applications of chitosan.In this paper,a xylan/chitosan composite was prepared in an ionic liquid system using glutaraldehyde as a crosslinking agent.The antioxidant activity,reducing capacity and metal ion chelating ability of the resulting composite were determined.The chemical structure and thermal stability were analyzed by FT-IR,XRD and TGA.The results showed that chitosan was successfully cross-linked with xylan by glutaraldehyde in the ionic liquid system.Compared to pure chitosan,the ABTS·+scavenging activity of the xylan/chitosan composite increased from 10.56%to 97.59%.After cross-linking with xylan by glutaraldehyde,the reducing power of xylan/chitosan composite increased from 0.054 to 2.109.The capacity of chelating metal ion of the composite also increased from 42.35%to 86.71%compared to that of pure chitosan.An underlying mechanism was proposed to account for the improvement of the chemical properties of the chitosan in the composite. | Hailong Gao Na Liu Shuzhen Ni Haixia Lin Yingjuan Fu | 2017 | Journal of Bioresources and Bioproducts2017，2，3： | 0 |
| 18 | Bending strain effects on the optical and optoelectric properties of GaN nanowires显示文摘Elastic strain has been an important method to regulate the electronic structures and physical properties of nanoscale semiconductors due to the promising potentials in improving the performance of their optoelectronic devices.Here,we report the investigation of bending strain effects on the optical and optoelectric properties of individual gallium nitride(GaN)nanowires(NWs).By charactering the near-band emission spectrum of individual GaN NWs at different bending strains with low temperature cathodoluminescence(CL),we reveal that the near-band emission splits into two peaks,where the low energy peak displays a linear redshift with increasing the bending strain while the high energy one shows a slight blueshift.Further localized ultraviolet(UV)photoresponse measurements illustrate that the photoresponse of the GaN NWs shows a linear increase with the bending train,and the maximum enhancement is more than two orders of magnitude.The experimental observations are well interpreted by theoretical calculations on the strain modulation on the electronic band structure of GaN combined with analysis of carrier dynamics and optical waveguide effect in the bending strain field.Our results not only shed light on the bending strain effects on the optical and optoelectric properties of semiconductors,but also hold potential to help the future design of high performance nano-optoelectric devices. | Xuewen Fu Haixia Nie Zepeng Sun Min Feng Xiang Chen Can Liu Fang Liu Dapeng Yu Zhimin Liao | 2022 | Nano Research2022，15，5： | 0 |
| 19 | Machine learning assisted prediction of charge transfer properties in organic solar cells by using morphology-related descriptors显示文摘Charge transfer and transport properties are crucial in the photophysical process of exciton dissociation and recombination at the donor/acceptor(D/A)interface.Herein,machine learning(ML)is applied to predict the charge transfer state energy(ECT)and identify the relationship between ECT and intermolecular packing structures sampled from molecular dynamics(MD)simulations on fullerene-and non-fullerene-based systems with different D/A ratios(RDA),oligomer sizes,and D/A pairs.The gradient boosting regression(GBR)exhibits satisfactory performance(r=0.96)in predicting ECT withπ-packing related features,aggregation extent,backbone of donor,and energy levels of frontier molecular orbitals.The charge transport property affected byπ-packing with different RDA has also been investigated by space-charge-limited current(SCLC)measurement and MD simulations.The SCLC results indicate an improved hole transport of non-fullerene system PM6/Y6 with RDA of 1.2:1 in comparison with the 1:1 counterpart,which is mainly attributed to the bridge role of donor unit in Y6.The reduced energetic disorder is correlated with the improved miscibility of polymer with RDA increased from 1:1 to 1.2:1.The morphology-related features are also applicable to other complicated systems,such as perovskite solar cells,to bridge the gap between device performance and microscopic packing structures. | Lulu Fu Haixia Hu Qiang Zhu Lifeng Zheng Yuming Gu Yaping Wen Haibo Ma Hang Yin Jing Ma | 2023 | Nano Research2023，16，2： | 0 |
| 20 | CASCIRE surveillance network and work on avian influenza viruses显示文摘Studies on influenza virus by Chinese Academy of Sciences(CAS)could be traced back as early as 2005 by the CAS Key Laboratory of Pathogenic Microbiology and Immunology(CASPMI),who discovered that Qinghai-like Clade 2.2H5N1 subtype highly pathogenic avian influenza virus(HPAIV)first caused severe outbreak in wild birds in Qinghai Lake(Liu et al.,2005). | Yuhai Bi Weifeng Shi Jianjun Chen Quanjiao Chen Zhenghai Ma Gary Wong Wenxia Tian Renfu Yin Guanghua Fu Yongchun Yang William J.Liu Chuansong Quan Qianli Wang Shenghu He Xiangdong Li Qianfeng Xia Lixin Wang Zhaohui Pan Laixing Li Hong Li Wen Xu Ying Luo Hui Zeng Lianpan Dai Haixia Xiao Kirill Sharshov Alexander Shestopalov Yi Shi Jinghua Yan Xuebing Li Yingxia Liu Fumin Lei Wenjun Liu George F.Gao | 2017 | Science China(Life Sciences)2017，60，12： | 0 |
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