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| 1 | Therapeutic imaging window of cerebral infarction revealed by multisequence magnetic resonance imaging An animal and clinical study显示文摘In this study, we established a Wistar rat model of right middle cerebral artery occlusion and observed pathological imaging changes (T2-weighted imaging [T2WI], T2FLAIR, and diffusion-weighted imaging [DWI]) following cerebral infarction. The pathological changes were divided into three phases: early cerebral infarction, middle cerebral infarction, and late cerebral infarction. In the early cerebral infarction phase (less than 2 hours post-infarction), there was evidence of intracellular edema, which improved after reperfusion. This improvement was defined as the ischemic penumbra. In this phase, a high DWI signal and a low apparent diffusion coefficient were observed in the right basal ganglia region. By contrast, there were no abnormal T2WI and T2FLAIR signals. For the middle cerebral infarction phase (2-4 hours post-infarction), a mixed edema was observed. After reperfusion, there was a mild improvement in cell edema, while the angioedema became more serious. A high DWI signal and a low apparent diffusion coefficient signal were observed, and some rats showed high T2WI and T2FLAIR signals. For the late cerebral infarction phase (4-6 hours post-infarction), significant angioedema was visible in the infarction site. After reperfusion, there was a significant increase in angioedema, while there was evidence of hemorrhage and necrosis. A mixed signal was observed on DWI, while a high apparent diffusion coefficient signal, a high T2WI signal, and a high T2FLAIR signal were also observed. All 86 cerebral infarction patients were subjected to T2WI, T2FLAIR, and DWI. MRI results of clinic data similar to the early infarction phase of animal experiments were found in 51 patients, for which 10 patients (10/51) had an onset time greater than 6 hours. A total of 35 patients had MRI results similar to the middle and late infarction phase of animal experiments, of which eight patients (8/35) had an onset time less than 6 hours. These data suggest that defining the 'therapeutic time window' as the time 6 hours after infarction may not be suitable for all patients. Integrated application of MRI sequences including T2WI, T2FLAIR, DW-MRI, and apparent diffusion coefficient mapping should be used to examine the ischemic penumbra, which may provide valuable information for identifying the 'therapeutic time window'. | Hong Lu Hui Hu Zhanping He Xiangjun Han Jing Chen Rong Tu | 2012 | Neural Regeneration Research2012，7，31： | 16 |
| 2 | Gene interference regulates aquaporin-4 expression in swollen tissue of rats with cerebral ischemic edema Correlation with variation in apparent diffusion coefficient显示文摘To investigate the effects of mRNA interference on aquaporin-4 expression in swollen tissue of rats with ischemic cerebral edema, and diagnose the significance of diffusion-weighted MRI, we injected 5 μL shRNA- aquaporin-4 (control group) or siRNA- aquaporin-4 solution (1:800) (RNA interference group) into the rat right basal ganglia immediately before occlusion of the middle cerebral artery. At 0.25 hours after occlusion of the middle cerebral artery, diffusion-weighted MRI displayed a high signal; within 2 hours, the relative apparent diffusion coefficient decreased markedly, aquaporin-4 expression increased rapidly, and intracellular edema was obviously aggravated; at 4 and 6 hours, the relative apparent diffusion coefficient slowly returned to control levels, aquaporin-4 expression slightly increased, and angioedema was observed. In the RNA interference group, during 0.25- 6 hours after injection of siRNA- aquaporin-4 solution, the relative apparent diffusion coefficient slightly fluctuated and aquaporin-4 expression was upregulated; during 0.5-4 hours, the relative apparent diffusion coefficient was significantly higher, while aquaporin-4 expression was significantly lower when compared with the control group, and intracellular edema was markedly reduced; at 0.25 and 6 hours, the relative apparent diffusion coefficient and aquaporin-4 expression were similar when compared with the control group; obvious angioedema remained at 6 hours. Pearson's correlation test results showed that aquaporin-4 expression was negatively correlated with the apparent diffusion coefficient (r = -0.806, P < 0.01). These findings suggest that upregulated aquaporin-4 expression is likely to be the main molecular mechanism of intracellular edema and may be the molecular basis for decreased relative apparent diffusion coefficient. Aquaporin-4 gene interference can effectively inhibit the upregulation of aquaporin-4 expression during the stage of intracellular edema with time-effectiveness. Moreover, diffusion-weighted MRI can accurately detect intracellular edema. | Hui Hu Hong Lu Zhanping He Xiangjun Han Jing Chen Rong Tu | 2012 | Neural Regeneration Research2012，7，21： | 14 |
| 3 | Cloning and Identification of Novel MicroRNAs from Rat Hippocampus显示文摘MicroRNAs (miRNAs ) 是小规章的分子 post-transcriptionallysuppressingmRNA 活动。在各种各样的有机体的许多 miRNAs 被克隆但是许多未知 miRNAs 遗体到我们描述从老鼠马头鱼尾的怪兽克隆六新 miRNAs 的 beidentified.Here。在他们之中,四没在老鼠米尔底被发现,但是与他们的人或老鼠相当或相同的事物相同,因此,他们被指定为 rno-miR-92b , rno-miR-146b , rno-let-7g ,和 rno-miR-551b.The 另外的二从 rno-miR-330 和 rno-miR-384 的已知的 miRNA 先锋的另外的手臂被导出,并且没在所有有机体的米尔底被发现。他们被指定为 rno-miR-330 * 并且这些 miRNAs 的 rno-miR-384 *.The 表示被跟踪 RNA 和教材扩展证实即时 reversetranscription 聚合酶链反应。这六 miRNAs 被表示在在马头鱼尾的怪兽显著地高级比在另外的纸巾包括服的外皮,心,肝,肺 andkidney.miR-384 * 是比在老鼠马头鱼尾的怪兽的 miR-384 更丰富的 10 次,但是小差别在 miR-330 之间被发现 * 并且在一样的织物的 miR-330 表示。 | Xiangjun HE Qi ZHANG Yujing LIU Xiuying PAN | 2007 | Acta Biochimica et Biophysica Sinica2007，39，9： | 11 |
| 4 | The influence of water-based drilling fluid on mechanical property of shale and the wellbore stability显示文摘Because of high cost and pollution of oil-based drilling fluid,the water-based drilling fluid is increasingly used now.However,bedding planes and micro-cracks are rich in shale formation.When water-based drilling fluid contacts formation rock,it causes the propagation of crack and invasion of drilling fluid,which decrease shale strength and cause wellbore instability.In this paper,we analyzed influence of water-based drilling fluid on shale strength and failure mode by mechanics experiment.Based on those experimental results,considering the effect of bedding plane and drilling time,we established modeling of wellbore stability for shale formation.The result from this model indicates that in certain azimuth of horizontal well,collapsing pressure increases dramatically due to shale failure along with bedding plane.In drilling operation,those azimuths are supposed to be avoided.This model is applicable for predication of collapsing pressure in shale formation and offers reference for choosing suitable mud weight. | Shu He Lixi Liang Yinjin Zeng Yi Ding Yongxue Lin Xiangjun Liu | 2016 | Petroleum2016，2，1： | 6 |
| 5 | The potential of Clostridium autoethanogenum,a new single cell protein,in substituting fish meal in the diet of largemouth bass(Micropterus salmoides):Growth,feed utilization and intestinal histology显示文摘This study investigated the effects of replacing fishmeal with Clostridium autoethanogenum protein(CAP)on growth,feed utilization and intestinal histology of largemouth bass(Micropterus salmoides).The control diet contained 350 g/kg fishmeal,and then dietary fishmeal was decreased to 300 g/kg,250 g/kg,200 g/kg,150 g/kg and 100 g/kg by CAP inclusion,respectively(FM-30,FM-25,FM-20,FM-15,FM-10).The largemouth bass(110.0±1.0 g)were fed one of the six diets for 8 weeks.The groups of FM-30,FM-25 and FM-20 showed the similar weight gain(WG)and feed conversion ratio(FCR)to the control(P>0.05),while the WG of FM-10 group was decreased by 15.9%,and FCR increased by 0.12 when compared to the control(P<0.05).FM-15 and FM-10 groups had lower protein retention,lower villus height in intestine and higher serum malondialdehyde content than the control(P<0.05).The protease activity in FM-20,FM-15 and FM-10 groups was lower than that in the control(P<0.05),but no difference was observed in crude protein digestibility among groups(P>0.05).In conclusion,CAP could replace 150 g/kg fishmeal in diet(350 g/kg fishmeal)without adverse effects on growth,feed utilization and intestinal histology of largemouth bass. | Pinxian Yang Xiaoqin Li Bowen Song Ming He Chaoyi Wu Xiangjun Leng | 2023 | Aquaculture and Fisheries2023，8，1： | 6 |
| 6 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 7 | DNA damage stress induces the dissociation of Smurf1/2 from MDM2 in a slow manner显示文摘The tumor suppressor p53 locates at the key point of cell growth or apoptosis balance, and the expression level of p53 is tightly controlled by ubiquitin ligases including MDM2. Upon DNA damage stresses, p53 was accumulated and activated, leading to cell cycle arrest or apoptosis. We previously showed that Smad ubiquitylation regulatory factor 1/2 (Smurf1/2) promotes p53 degradation by interacting with and stabilizing MDM2, and consequently enhancing MDM2-mediated ubiquitylation of p53. However, it is unclear how the Smurf1-MDM2 interaction is regulated in response to DNA damage stress. Here, we show that in response to etoposide treatment Smurf1 dissociates from MDM2, resulting in MDM2 destabilization and p53 accumulation. The negative regulation of Smurf1 on apoptosis is released. Notably, this dissociation is a slow process rather than a rapid response, implicating high expression of Smurf1 might confer the resistance against p53 activation. Consistent with this notion, we observed that Smurf1/2 ligases are highly expressed in colon cancer, esophageal squamous cell carcinoma and pancreatic cancer tissues, suggesting the oncogenic tendency of Smurf1/2. | NIE Jing LIU Lin ZHAO XiaoHang XIE Ping ZHOU PingKun XING GuiChun LIU XiangJun HE FuChu HAN WeiDong ZHANG LingQiang | 2011 | Chinese Science Bulletin2011，56，30： | 2 |
| 8 | Myristica fragrans promotes ABCA1 expression and cholesterol efflux in THP-1-derived macrophages显示文摘Myristica fragrans is a traditional herbal medicine and has been shown to alleviate the development of atherosclerosis.However,the anti-atherogenic mechanisms of M.fragrans are still to be addressed.In this study,we explored the effect of M.fragrans on lipid metabolism and inflammation and its mechanisms in THP-1-derived macrophages.The quantitative polymerase chain reaction and western blot analysis results showed that M.fragrans promotes cholesterol efflux from THP-1-derived macrophages and reduces intracellular total cholesterol,cholesterol ester,and free cholesterol contents in a dose-and a time-dependent manner.Further study found that liver X receptor alpha(LXRα)antagonist GGPP significantly blocked the upregulation of ABCA1 expression with M.fragrans treatment.In addition,chromatin immunoprecipitation assay confirmed that GATA binding protein 3(GATA3)can bind to the LXRαpromoter,and inhibition of GATA3 led to the downregulation of LXRαand ATP-binding cassette subfamily A member 1 expression.Furthermore,M.fragrans reduced lipid accumulation,followed by decreasing tumor necrosis factor-α,interleukin(IL)-6,and IL-1βand increasing IL-10 produced by THP-1-derived macrophages.Therefore,M.fragrans is identified as a valuable therapeutic medicine for atherosclerotic cardiovascular disease. | Shangming Liu Jiahui Gao Linhao He Zhenwang Zhao Gang Wang Jin Zou Li Zhou Xiangjun Wan Shilin Tang Chaoke Tang | 2021 | Acta Biochimica et Biophysica Sinica2021，53，1： | 2 |
| 9 | microRNA-320a inhibits tumor invasion by targeting neuropilin 1 andis associated with liver metastasis in colorectal cancer显示文摘 | Yujun Zhang Xiangjun He Yulan Liu Yingjiang Ye Hui Zhang Peiying He Qi Zhang Lingyi Dong Yujing Liu Jianqiang Dong | 2012 | Oncology Reports2012，，3： | 2 |
| 10 | Web-based Product Development and Simulation with Virtual Reality显示文摘 | Bangjun Gu Jian Sun Hanwu He | 2006 | Journal of Computational Information (S1553-9105)2006，2，1： | 1 |
| 11 | Web-based Product Development and Simulation with Virtual Reality显示文摘 | ZOU Xiangjun GU Bangjun SUN Jian HE Hanwu | 2006 | JOURNAL OF COMPUTATIONAL INFORMATION SYSTEMS2006，2，1： | 1 |
| 12 | 来源于人膀胱癌细胞株EJ的HSP70负载的DC所激发的特异性CTL细胞的体外应答效应(英文)显示文摘Objective: To investigate whether human dendritic cells (DC) derived from peripheral blood mononuclear cells (PBMC), which were pulsed by heat shock protein 70 (HSP70) isolated from human bladder tumor cell lines of EJ, were able to induce peptide specific cytotoxic T-lymphocytes (CTL) response in vitro and give the experimental foundation for the future clinical trials of immunotherapy in bladder tumor. Methods: The EJ-derived HSP70 co-cultured with DC from the healthy volunteers' PBMC, along with the crude lysate (the supernatant before HSP70 purification) from EJ cells were used as the experimental groups and DC not pulsed by any tumor cells antigen were the blank control. The autologous T-lymphocytes were added into the above various DC groups, and after incubation, the stimulation indexes (SI) and interferon-γ (IFN-γ) were detected to evaluate the immune activities of various DC groups. The killing effects of CTL to target cells, EJ and Hela cells, were determined with 51Cr releasing test. Results: Both DC/HSP70 and DC/the crude lysate could effectively activate CTL in vitro and kill target cells EJ. The killing effect of DC/HSP70 to EJ was much stronger than DC/the crude lysate (the supernatant before HSP70 purification) (P < 0.05). DC without any tumor cell antigens had a lower killing power to EJ. Meanwhile, DC/ HSP70 had little killing power to Hela non-relevant to bladder tumor histopathologically as compared with EJ cells (P < 0.05). Conclusion: The DC pulsed by HSP70 derived from the autologous tumor cells could induce a peptide complexes specific CTL response to tumor cells, and the CTL response induced by the DC/HSP70 was stronger, which display the basis of the possible clinical application of DC/HSP70 for bladder tumor. | Lingfeng He Jianhua Wang Xiaofeng Wang Xiangjun He Zheng Yan Kexin Xu Kaopeng Guan Shukun Hou | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，12： | 1 |
| 13 | INTERFACIAL REACTIONS OF METAL FILMS WITH AlN SUBSTRATE显示文摘INTERFACIALREACTIONSOFMETALFILMSWITHAlNSUBSTRATEHeXiangjun;TaoKun;FanYudian(DepartmentofMaterialsScienceandEngineering,Tsingh... | He Xiangjun Tao Kun Fan Yudian (Department of Materials Science and Engineering, Tsinghua University, Beijing 100084) | 1996 | 中国有色金属学会会刊:英文版1996，6，2： | 1 |
| 14 | Studies of interaction of copper and zinc ions with Alzheimer' s Aβ ( 1 - 16) using surface plasmon resonance spectrometer 显示文摘 | Yao Fujun He Jianfeng Li Xiangjun | 2012 | Sensors and Actuators B: Chemical2012，161，： | 1 |
| 15 | Optomechanical soft metamaterials显示文摘We present a new type of optomechanical soft metamaterials, which is different from conventional mechanical metamaterials, in that they are simple isotropic and homogenous materials without resorting to any complex nano/microstructures. This metamaterial is unique in the sense that its responses to uniaxial forcing can be tailored by programmed laser inputs to manifest different nonlinear constitutive behaviors, such as monotonic, S-shape, plateau, and non-monotonic snapping performance. To demonstrate the novel metamaterial, a thin sheet of soft material impinged by two counterpropagating lasers along its thickness direction and stretched by an in-plane tensile mechanical force is considered. A theoretical model is formulated to characterize the resulting optomechanical behavior of the thin sheet by combining the nonlinear elasticity theory of soft materials and the optical radiation stress theory. The optical radiation stresses predicted by the proposed model are validated by simulations based on the method of finite elements. Programmed optomechanical behaviors are subsequently explored using the validated model under different initial sheet thicknesses and different optical inputs, and the first-and second-order tangential stiffness of the metamaterial are used to plot the phase diagram of its nonlinear constitutive behaviors.The proposed optomechanical soft metamaterial shows great potential in biological medicine, microfluidic manipulation,and other fields. | Xiangjun Peng Wei He Yifan Liu Fengxian Xin Tian Jian Lu | 2017 | Acta Mechanica Sinica2017，33，3： | 1 |
| 16 | Exploration on building of visualization platform to innovate business operation pattern of supply chain finance显示文摘 | HE XIANGJUN TANG LINGYUN | 2012 | Physics Procedia2012，，18： | 1 |
| 17 | Potassic magmatism in western Sichuan and Yunnan provinces, SE Tibet, China: Petrological and geochemical constraints on petrogenesis 显示文摘 | Guo Zhengfu Hertogen J Liu Jiaqi Pasteels P Boven A Punzalan L He Huaiyu Luo Xiangjun Zhang Wenhua | 2005 | J Petrol2005，46，1： | 1 |
| 18 | Web-based Product Development and Simulation with Virtual Reality显示文摘 | BangJnn Gu Jian Sun Hanwu He | 2006 | Journal of Computational Information Systems (S1553-9105)2006，2，1： | 1 |
| 19 | Formation of AlN films by Al evaporation with nitrogen ion beam bombardment显示文摘 | HE Xiangjun YANG Size TAO Kun | 1997 | Mater Chem Phy1997，51，： | 1 |
| 20 | Bridging small-gap peripheral nerve defects using biodegradable chintin conduit with cultured cells in rats 显示文摘 | Peixun Zhang Xiangjun He Fuqiang Zhao | 2005 | Journal of reconstructive microsurgery2005，8，21： | 1 |
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