|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Therapeutic imaging window of cerebral infarction revealed by multisequence magnetic resonance imaging An animal and clinical study显示文摘In this study, we established a Wistar rat model of right middle cerebral artery occlusion and observed pathological imaging changes (T2-weighted imaging [T2WI], T2FLAIR, and diffusion-weighted imaging [DWI]) following cerebral infarction. The pathological changes were divided into three phases: early cerebral infarction, middle cerebral infarction, and late cerebral infarction. In the early cerebral infarction phase (less than 2 hours post-infarction), there was evidence of intracellular edema, which improved after reperfusion. This improvement was defined as the ischemic penumbra. In this phase, a high DWI signal and a low apparent diffusion coefficient were observed in the right basal ganglia region. By contrast, there were no abnormal T2WI and T2FLAIR signals. For the middle cerebral infarction phase (2-4 hours post-infarction), a mixed edema was observed. After reperfusion, there was a mild improvement in cell edema, while the angioedema became more serious. A high DWI signal and a low apparent diffusion coefficient signal were observed, and some rats showed high T2WI and T2FLAIR signals. For the late cerebral infarction phase (4-6 hours post-infarction), significant angioedema was visible in the infarction site. After reperfusion, there was a significant increase in angioedema, while there was evidence of hemorrhage and necrosis. A mixed signal was observed on DWI, while a high apparent diffusion coefficient signal, a high T2WI signal, and a high T2FLAIR signal were also observed. All 86 cerebral infarction patients were subjected to T2WI, T2FLAIR, and DWI. MRI results of clinic data similar to the early infarction phase of animal experiments were found in 51 patients, for which 10 patients (10/51) had an onset time greater than 6 hours. A total of 35 patients had MRI results similar to the middle and late infarction phase of animal experiments, of which eight patients (8/35) had an onset time less than 6 hours. These data suggest that defining the 'therapeutic time window' as the time 6 hours after infarction may not be suitable for all patients. Integrated application of MRI sequences including T2WI, T2FLAIR, DW-MRI, and apparent diffusion coefficient mapping should be used to examine the ischemic penumbra, which may provide valuable information for identifying the 'therapeutic time window'. | Hong Lu Hui Hu Zhanping He Xiangjun Han Jing Chen Rong Tu | 2012 | Neural Regeneration Research2012，7，31： | 16 |
| 2 | Gene interference regulates aquaporin-4 expression in swollen tissue of rats with cerebral ischemic edema Correlation with variation in apparent diffusion coefficient显示文摘To investigate the effects of mRNA interference on aquaporin-4 expression in swollen tissue of rats with ischemic cerebral edema, and diagnose the significance of diffusion-weighted MRI, we injected 5 μL shRNA- aquaporin-4 (control group) or siRNA- aquaporin-4 solution (1:800) (RNA interference group) into the rat right basal ganglia immediately before occlusion of the middle cerebral artery. At 0.25 hours after occlusion of the middle cerebral artery, diffusion-weighted MRI displayed a high signal; within 2 hours, the relative apparent diffusion coefficient decreased markedly, aquaporin-4 expression increased rapidly, and intracellular edema was obviously aggravated; at 4 and 6 hours, the relative apparent diffusion coefficient slowly returned to control levels, aquaporin-4 expression slightly increased, and angioedema was observed. In the RNA interference group, during 0.25- 6 hours after injection of siRNA- aquaporin-4 solution, the relative apparent diffusion coefficient slightly fluctuated and aquaporin-4 expression was upregulated; during 0.5-4 hours, the relative apparent diffusion coefficient was significantly higher, while aquaporin-4 expression was significantly lower when compared with the control group, and intracellular edema was markedly reduced; at 0.25 and 6 hours, the relative apparent diffusion coefficient and aquaporin-4 expression were similar when compared with the control group; obvious angioedema remained at 6 hours. Pearson's correlation test results showed that aquaporin-4 expression was negatively correlated with the apparent diffusion coefficient (r = -0.806, P < 0.01). These findings suggest that upregulated aquaporin-4 expression is likely to be the main molecular mechanism of intracellular edema and may be the molecular basis for decreased relative apparent diffusion coefficient. Aquaporin-4 gene interference can effectively inhibit the upregulation of aquaporin-4 expression during the stage of intracellular edema with time-effectiveness. Moreover, diffusion-weighted MRI can accurately detect intracellular edema. | Hui Hu Hong Lu Zhanping He Xiangjun Han Jing Chen Rong Tu | 2012 | Neural Regeneration Research2012，7，21： | 14 |
| 3 | Promoting effect of rhenium on catalytic performance of Ru catalysts in hydrogenolysis of glycerol to propanediol 显示文摘 | Ma Lan He Dehua Li Zhanping | 2008 | Catal Commun2008，9，15： | 1 |
| 4 | Antitumor Response of Anti-B7-H3 CAR-T Cells with Humanized scFv in Solid Tumors显示文摘We prepared a humanized alternative B7-H3 CAR(B7-H3-haCAR-T)targeting B7-H3 which contained the humanized singl-chain varinlde fragment(scFv)from humanized 8H9 Monoclonal Antibody(hu8H9m Ab).The antitumor effects of B7-H3-haCAR-T cells were evaluated in B7-H3 overexpressed tumor cells(in vitro)and B7-H3 xenograft models(in vivo).The specific tumour killing ability of B7-H3-haCAR-T in overexpressing B7-H3 tumour cells was verified by cytotoxicity and ELISA tests.In addition,B7-H3 haCAR-T cells were also noted to suppress the tumour growth remarkably well in the xenograft murine models,and the survival time in the haCAR-T cell treatment group was appreciably longer than that in the control group.The specific recognition and highly efficient tumoricidal behavior of B7-H3 haCAR-T provide a basis for future clinical studies with humanized scFv-transduced CAR-T cells targeting solid tumors. | SI Ke XU Huantian YE Zheng JAFFAR ALI Doulathunnisa HE Cong DING Bo YUAN Shubin DAI Zhu LI Zhanping SUN Bo SHEN Yang XIAO Zhongdang | 2022 | Wuhan University Journal of Natural Sciences2022，27，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员