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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | A finite element modeling of posterior atlantoaxial fixation and biomechanical analysis of C2 intralaminar screw fixation显示文摘 | Ma Xuexiao Peng Xianbo Xiang Hongfei Zhang Yan Zhang Guoqing Chen Bohua | 2014 | Chinese Medical Journal2014，，7： | 15 |
| 3 | Magnetoresistance enhancement and temperature stability of magnetoresistance in La_(1-x)(Sr_(1-y)Na_y)_xMnO_3显示文摘A series of the samples La_(1-x)(Sr_(1-y)Na_y)_xMnO_3(y=0.0,0.2,0.4,0.6,0.8,1.0) were prepared by the solid-state reaction method.Magnetoresistance enhancement and temperature stability of magnetoresistance in the system La_(1-x)(Sr_(1-y)Na_y)_xMnO_3 with unchanged Mn^(3+)/Mn^(4+) ratio through the doping of both monovalent and divalent elements at A site were studied through the measurements of X-ray diffraction(XRD) patterns,resistivity-temperature(ρ-T) curves and magnetoresistance-temperature(MR-T) curves.The results indicate that with the increase of Na doping amount,the peak value of MR increases,and it increases from 12.4% for y=0.2 to 50.6% for y=1.0 in the magnetic field B=0.8 T;ρ-T curves exhibit the double-peak phenomenon,which comes from the competition between the resistivity of surface phase and that of body phase;for the sample of y=0.8,MR increases slowly from 8.3% to 9.4% in the temperature range from 259 to 179 K,and MR is so stable in such a wide temperature range,which provides reference for the research on the temperature stability of MR. | Wang, Guiying Yan, Guoqing Yang, Jie Wang, Wenqi Tang, Yonggang Song, Qixiang Zhang, Mingyu Peng, Zhensheng | 2012 | Rare Metals2012，31，4： | 13 |
| 4 | Integrated optimization analyses of aerodynamic/stealth characteristics of helicopter rotor based on surrogate model显示文摘Based on computational fluid dynamics(CFD)method,electromagnetic high-frequency method and surrogate model optimization techniques,an integration design method about aerodynamic/stealth has been established for helicopter rotor.The developed integration design method is composed of three modules:integrated grids generation(the moving-embedded grids for CFD solver and the blade grids for radar cross section(RCS)solver are generated by solving Poisson equations and folding approach),aerodynamic/stealth solver(the aerodynamic characteristics are simulated by CFD method based upon Navier–Stokes equations and Spalart–Allmaras(S–A)turbulence model),and the stealth characteristics are calculated by using a panel edge method combining the method of physical optics(PO),equivalent currents(MEC)and quasi-stationary(MQS),and integrated optimization analysis(based upon the surrogate model optimization technique with full factorial design(FFD)and radial basis function(RBF)),an integrated optimization analyses on aerodynamic/stealth characteristics of rotor are conducted.Firstly,the scattering characteristics of the rotor with different blade-tip swept and twist angles have been carried out,then time–frequency domain grayscale with strong scattering regions of rotor have been given.Meanwhile,the effects of swept-tip and twist angles on the aerodynamic characteristic of rotor have been performed.Furthermore,by choosing suitable object function and constraint condition,the compromised design about swept and twist combinations of rotor with high aerodynamic performances and low scattering characteristics has been given at last. | Jiang Xiangwen Zhao Qijun Zhao Guoqing Li Peng | 2015 | Chinese Journal of Aeronautics2015，28，3： | 12 |
| 5 | First mass measurement of short-lived nuclides at HIRFL-CSR显示文摘Recent commissioning of the HIRFL-CSR has demonstrated its ability to perform direct mass measurement for short-lived nuclides. Projectile fragments produced by the 78Kr ions at 481.88 MeV/u were separated with the new radioactive beam line in Lanzhou (RIBLL2), and injected into and stored in the experimental storage ring (CSRe). By operating the CSRe as an isochronous mass spectrometry, a typical mass resolution around 2.0×10?5 has been achieved. The masses for 63Ge, 65As and 67Se were measured for the first time. The measured masses are compared with theoretical predictions and the location of the proton drip-line for As isotopes is discussed. The implication of the 65As mass in the astrophysical rapid proton capture process has also been addressed. | XU HuShan TU XiaoLin YUAN YouJin XIA JiaWen ZANG YongDong WANG Meng MAO RuiShi HU ZhengGuo MEI Bo SHUAI Peng ZHU ZhiChao TANG ShuWen GENG Peng LIU Yong ZHAO TieCheng ZHANG XueYing YANG JianCheng MAO LiJun ZHANG YuHu ZHOU XiaoHong HUANG WenXue XIAO GuoQing ZHAO HongWei ZHAN WenLong | 2009 | Chinese Science Bulletin2009，54，24： | 11 |
| 6 | Low-field magnetoresistance of (1-x)La_(0.6)Dy_(0.1)Sr_(0.3)MnO_3/0.5x (Sb_2O_3) composite system under different sintering temperatures of matrix显示文摘A series of (1-x)La0.6Dy0.1Sr0.3MnO3/0.5x(Sb2O3)(x=0.15) samples were prepared by the solid-state reaction method, and the influence of sintering temperature of the matrix on low-field magnetoresistance of (1-x)La0.6Dy0.1Sr0.3MnO3/0.5x (Sb2O3) was studied through the measurements of X-ray diffraction (XRD) patterns, scanning electron microscope (SEM) image, resistivity-temperature (ρ-T) curves, and magnetoresistance-temperature (MR-T) curves. The results indicate that for the samples with low sintering temperature of the matrix, lowfield magnetoresistance effect appears on the whole temperature range and can be explained by grain boundary effect; for the sample with high sintering temperature of the matrix, intrinsic magnetoresistance peak appears on the high-temperature range, low-field magnetore-sistance effect appears on low temperature range, and the magnetoresistance in the magnetic field of 0.2 T and on the comparatively large temperature range between 280 K and 225 K hardly changes with temperature and remains at 4.8%, which can be explained by the competition between the intrinsic magnetoresistance induced by double-exchange function inside grains and the tunneling magnetoresis-tance (TMR) induced by grain boundary effect. The temperature stability of magnetoresistance is beneficial to the practical applications of MR. | Yang, Jie Yan, Guoqing Wang, Guiying Tang, Yonggang Song, Qixiang Zhang, Mingyu Peng, Zhensheng | 2012 | Rare Metals2012，31，3： | 7 |
| 7 | Elastic Scattering of 7Be on Pb Target显示文摘 | Yang Yanyun Wang Jiansong Wang Qi Huang Meirong Ma Junbing Ma Peng Cao Xiguang Han Jianlong Bai Zhen Jin Shilong Hu Qiang Jin Lei Chen Jiangbo Zhang Xueying Chen Ruofu Hu Zhengguo Xu Shiwei Zhen Chuan Xu Zhiguo Xu Hushan Duan Liming Sun Zhiyu Chen Zhiqiang He Jianjun Li Songlin Lei Xiangguo Fu Fen Zhang Xueheng Yuan Xiaohua Du Chengming Yan Xinshuang Hu Jun Tang Shuwen Ye Ruiping Yang Kun Shi Fudong Zhang Wei Chen Ze Li Long Xu Xing Liu Longxiang Zhang Liyong Lin Qingyun Gou Boxing Xu Ge Zhou Xionghong Zhang Yuhu Xiao Guoqing | 2011 | IMP & HIRFL Annual Report2011，，1： | 4 |
| 8 | Magnetic properties of perpendicularly orientated L1_0 FePt nanoparticles显示文摘L10 FePt films were deposited on MgO (001) substrates heated to 700°C by magnetron sputtering.Assisted by the misfit of lattice between film and substrate,strong (001) texture was formed.The film at nominal thickness t N=5 nm was composed of nanoparticles with a size of~70 nm,and showed a high coercivity of~105 kOe at 4.2 K.At t N=~50 nm,as the film changed from discontinuous to continuous,the coercivity dropped about one order of magnitude.Micromagnetic simulation implies that the magnetization reversal is a vortex-like nuclear type.The ideal coercivity of a separated single-domain L10 FePt nanoparticle with a size of 70 nm×70 nm×5 nm is~121 kOe.This tells us that the experimental coercivity has nearly reached the limit of ideal single crystalline nanoparticles. | MO XiaoJing XIANG Hui LI GuoQing CHEN Peng XIONG ZuHong WANG JunZhong ISHIO ShunJi SMTO Hitoshi SHIMA ToshiYuki TAKANASHI KoKi | 2010 | Chinese Science Bulletin2010，55，8： | 3 |
| 9 | A Versatile Surface Bioengineering Strategy Based on Mussel-Inspired and Bioclickable Peptide Mimic显示文摘In this work,we present a versatile surface engineering strategy by the combination of mussel adhesive peptide mimicking and bioorthogonal click chemistry.The main idea reflected in this work derived from a novel mussel-inspired peptide mimic with a bioclickable azide group(i.e.,DOPA_(4)-azide).Similar to the adhesion mechanism of the mussel foot protein(i.e.,covalent/noncovalent comediated surface adhesion),the bioinspired and bioclickable peptide mimic DOPA_(4)-azide enables stable binding on a broad range of materials,such as metallic,inorganic,and organic polymer substrates.In addition to the material universality,the azide residues of DOPA_(4)-azide are also capable of a specific conjugation of dibenzylcyclooctyne-(DBCO-)modified bioactive ligands through bioorthogonal click reaction in a second step.To demonstrate the applicability of this strategy for diversified biofunctionalization,we bioorthogonally conjugated several typical bioactive molecules with DBCO functionalization on different substrates to fabricate functional surfaces which fulfil essential requirements of biomedically used implants.For instance,antibiofouling,antibacterial,and antithrombogenic properties could be easily applied to the relevant biomaterial surfaces,by grafting antifouling polymer,antibacterial peptide,and NO-generating catalyst,respectively.Overall,the novel surface bioengineering strategy has shown broad applicability for both the types of substrate materials and the expected biofunctionalities.Conceivably,the“clean”molecular modification of bioorthogonal chemistry and the universality of mussel-inspired surface adhesion may synergically provide a versatile surface bioengineering strategy for a wide range of biomedical materials. | Yu Xiao Wenxuan Wang Xiaohua Tian Xing Tan Tong Yang Peng Gao Kaiqing Xiong Qiufen Tu Miao Wang Manfred FMaitz Nan Huang Guoqing Pan Zhilu Yang | 2020 | Research2020，，1： | 3 |
| 10 | White-light emission from InGaN/GaN quantum well microrings grown by selective area epitaxy显示文摘Monolithic white-light-emitting diodes(white LEDs) without phosphors are demonstrated using In GaN/GaN multiple quantum wells(MQWs) grown on GaN microrings formed by selective area epitaxy on SiO_2 mask patterns. The microring structure is composed of {1-101} semi-polar facets and a(0001) c-plane, attributed to favorable surface polarity and surface energy. The white light is realized by combining short and long wavelengths of electroluminescence emissions from In GaN /GaN MQWs on the {1-101} semi-polar facets and the(0001) c-plane,respectively. The change in the emission wavelengths from each microfacet is due to the In composition variations of the MQWs. These results suggest that white emission can possibly be obtained without using phosphors by combining emission light from microstructures. | Guofeng Yang Peng Chen Shumei Gao Guoqing Chen Rong Zhang Youdou Zheng | 2016 | Photonics Research2016，4，1： | 3 |
| 11 | Methylesterification of cell-wall pectin controls the diurnal flower-opening times in rice显示文摘Flowers are the core reproductive organ of plants, and flowering is essential for cross-pollination. Diurnal flower-opening time is thus a key trait influencing reproductive isolation, hybrid breeding, and thermostability in plants. However, the molecular mechanisms controlling this trait remain unknown. Here, we report that rice Diurnal Flower Opening Time 1 (DFOT1) modulates pectin methylesterase (PME) activity to regulate pectin methylesterification levels of the lodicule cell walls, which affect lodicule swelling to control diurnal flower-opening time. DFOT1 is specifically expressed in the lodicules, and its expression gradually increases with the approach to flowering but decreases with flowering. Importantly, a knockout of DFOT1 showed earlier diurnal flower opening. We demonstrate that DFOT1 interacts directly with multiple PMEs to promote their activity. Knockout of PME40 also resulted in early diurnal flower opening, whereas overexpression of PME42 delayed diurnal flower opening. Lower PME activity was observed to be associated with higher levels of pectin methylesterification and the softening of cell walls in lodicules, which contribute to the absorption of water by lodicules and cause them to swell, thus promoting early diurnal flower opening. Higher PME activity had the opposite effect. Collectively, our work uncovers a molecular mechanism underlying the regulation of diurnal flower-opening time in rice, which would help reduce the costs of hybrid breeding and improve the heat tolerance of flowering plants by avoiding higher temperatures at anthesis. | Mumei Wang Xiaopei Zhu Guoqing Peng Minglong Liu Shuqing Zhang Minghao Chen Shitang Liao Xiaoying Wei Peng Xu Xiyu Tan Fangping Li Zhichuan Li Li Deng Ziliang Luo Liya Zhu Shuai Zhao Dagang Jiang Jing Li Zhenlan Liu Xianrong Xie Shaokui Wang Aimin Wu Chuxiong Zhuang Hai Zhou | 2022 | Molecular Plant2022，15，6： | 2 |
| 12 | PITPNC1 promotes the thermogenesis of brown adipose tissue under acute cold exposure显示文摘Brown adipose tissue (BAT) plays an essential role in non-shivering thermogenesis. The phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1) is identified as a lipid transporter that reciprocally transfers phospholipids between intracellular membrane structures. However, the physiological significance of PITPNC1 and its regulatory mechanism remain unclear. Here, we demonstrate that PITPNC1 is a key player in thermogenesis of BAT. While Pitpnc1^(−/−) mice do not differ with wildtype mice in body weight and insulin sensitivity on either chow or high-fat diet, they develop hypothermia when subjected to acute cold exposure at 4℃. The Pitpnc1^(−/−) brown adipocytes exhibit defective β-oxidation and abnormal thermogenesis-related metabolism pathways in mitochondria. The deficiency of lipid mobilization in Pitpnc1^(−/−) brown adipocytes might be the result of excessive accumulation of phosphatidylcholine and a reduction of phosphatidic acid. Our findings have uncovered significant roles of PITPNC1 in mitochondrial phospholipid homeostasis and BAT thermogenesis. | Guoqing Tang Chengxin Ma Liangkui Li Shaoyan Zhang Fengsheng Li Jin Wu Yesheng Yin Qing Zhu Yan Liang Ru Wang He Huang Tong-Jin Zhao Hongyuan Yang Peng Li Feng-Jung Chen | 2022 | Science China(Life Sciences)2022，65，11： | 2 |
| 13 | Ubiquitinome Profiling Reveals the Landscape of Ubiquitination Regulation in Rice Young Panicles显示文摘Ubiquitination,an essential post-transcriptional modification(PTM),plays a vital role in nearly every biological process,including development and growth.Despite its functions in plant reproductive development,its targets in rice panicles remain unclear.In this study,we used proteome-wide profiling of lysine ubiquitination in rice(O.sativa ssp.indica)young panicles.We created the largest ubiquitinome dataset in rice to date,identifying 1638 lysine ubiquitination sites on916 unique proteins.We detected three conserved ubiquitination motifs,noting that acidic glutamic acid(E)and aspartic acid(D)were most frequently present around ubiquitinated lysine.Enrichment analysis of Gene Ontology(GO)annotations and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways of these ubiquitinated proteins revealed that ubiquitination plays an important role in fundamental cellular processes in rice young panicles.Interestingly,enrichment analysis of protein domains indicated that ubiquitination was enriched on a variety of receptorlike kinases and cytoplasmic tyrosine and serine-threonine kinases.Furthermore,we analyzed the crosstalk between ubiquitination,acetylation,and succinylation,and constructed a potential protein interaction network within our rice ubiquitinome.Moreover,we identified ubiquitinated proteins related to pollen and grain development,indicating that ubiquitination may play a critical role in the physiological functions in young panicles.Taken together,we reported the most comprehensive lysine ubiquitinome in rice so far,and used it to reveal the functional role of lysine ubiquitination in rice young panicles. | Liya Zhu Han Cheng Guoqing Peng Shuansuo Wang Zhiguo Zhang Erdong Ni Xiangdong Fu Chuxiong Zhuang Zexian Liu Hai Zhou | 2020 | Genomics, Proteomics & Bioinformatics2020，18，3： | 2 |
| 14 | Research on charge ordering and magnetic properties in La_(0.3)Ca_(0.7)Mn_(1-x)W_xO_3 system显示文摘The perovskite manganite sample La0.3Ca0.7Mn1-xWxO3 (x = 0.08, 0.12) was prepared by the solid-state reaction method. The effect of W doping on the Mn site to La0.3Ca0.7MnO3 charge ordering phase and the changing process of mag- netic properties were studied through the measurement of the M-T curve, M-H curves, and ESR curves of the sample. The results showed that when x = 0.08, the charge ordering (CO) phase exists in the system, the transition temperature TCO = 275 K, and the system exhibits PM when T > 275 K. The system transforms from spin-disordering paramagnetism to spin-ordering antiferromagnetism in the charge ordering state with the temperature decreasing from 275 K to 230 K. The long-range antiferromagnetism forms and AFM/CO states coexist between 230 K and 5 K. There is a little ferromagnetic component in the AFM/CO background in a low temperature range. When x = 0.12, the CO phase in the system has almost melted completely. There is a little remnant of the CO phase below 150 K. The system exhibits paramagnetism when T > 150 K and transforms from paramagnetism to ferromagnetism when T < 150 K. | PENG Zhensheng GUO Huanyin YAN Guoqing MAO Qiang | 2007 | Rare Metals2007，26，4： | 2 |
| 15 | Suppression of FHL2 Expression Induces Cell Differentiation and Inhibits Gastric and Colon Carcinogenesis显示文摘 | Jide Wang Yi Yang Harry H.X. Xia Qing Gu Marie C.M. Lin Bo Jiang Ying Peng Guoqing Li Xiaomeng An Yali Zhang Zehao Zhuang Zhenshu Zhang Hsiang Fu Kung Benjamin C.Y. Wong | 2007 | Gastroenterology2007，，3： | 1 |
| 16 | Uncertainties of thermal boundaries and soil properties on permafrost table of frozen ground in Qinghai-Tibet Plateau显示文摘In the permafrost regions of the Qinghai-Tibet Plateau(QTP),the permafrost table has a significant effect on the stability of geotechnical engineering.The thermal boundaries and soil properties are the key factors affecting the permafrost table.Complex geological environments and human activities can lead to the uncertainties of thermal boundaries and soil properties.In this paper,an array of field experiments and Monte Carlo(MC)simulations of thermal boundaries and soil properties are carried out.The coefficient of variation(COV),scale of fluctuation(SOF),and autocorrelation distance(ACD)of uncertainties of thermal boundaries and soil properties are investigated.A stochastic analysis method of the probabilistic permafrost table is then proposed,and the statistical properties of permafrost table on the QTP are computed by self-compiled program.The proposed stochastic analysis method is verified with the calculated and measured temperature observations.According to the relationship between ACD and SOF for the five theoretical autocorrelation functions(ACFs),the effects of ACF,COV,and ACD of soil properties and the COV of thermal boundaries on the permafrost tables are analyzed.The results show that the effects of different ACFs of soil properties on the standard deviation(SD)of permafrost table depth are not obvious.The SD of permafrost table depth increases with time,and the larger the COVs of thermal boundaries and soil properties,the deeper the SD of permafrost table;the longer the ACD of soil properties,the shallower the SD of permafrost table.This study can provide a reference for the stability analysis of geotechnical engineering on the QTP considering the uncertainties of thermal boundaries and soil properties. | Tao Wang Erxing Peng Lijiang Xia Guoqing Zhou Jianzhou Wang | 2021 | Journal of Rock Mechanics and Geotechnical Engineering2021，13，3： | 1 |
| 17 | Computationally Efficient Mining for Fuzzy Implication-based Association Rules in Quantitative Databases显示文摘 | Chen Guoqing Yan Peng Kerre E E | 2004 | International Journal of General Systems2004，33，2： | 1 |
| 18 | 显示文摘 | WANG Guiying a b YAN Guoqing a b YANG Jie a b WANG Wenqi a b TANG Yonggang a b SONG Qixiang a b ZHANG Mingyu a b and PENG Zhensheng a c a Anhui Key Laboratory of Spintronic and Nanometric Materials(Cultivating Base) Suzhou 234000 China b School of Mechani | 2012 | Rare Metals2012，，04： | 1 |
| 19 | Adaptivekernel independent component analysis and UV spectrom-etry applied to characterize the procedure for processingprepared rhubarb roots显示文摘 | Wang Guoqing Hou Zhenyu Peng Yang | 2011 | Analyst2011，136，： | 1 |
| 20 | Effect of equilibrium and dynamic surface activity of surfactant on foam transport in porous medium 显示文摘 | LI Ying ZHANG Peng ZHAO Guoqing | 2006 | Colloids Surf A: Physicochem Eng Aspects2006，272，12： | 1 |
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