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| 1 | Temperature effect and thermal impact in lithium-ion batteries: A review显示文摘Lithium-ion batteries, with high energy density(up to 705 Wh/L) and power density(up to 10,000 W/L), exhibit high capacity and great working performance. As rechargeable batteries, lithium-ion batteries serve as power sources in various application systems. Temperature, as a critical factor, significantly impacts on the performance of lithium-ion batteries and also limits the application of lithium-ion batteries. Moreover, different temperature conditions result in different adverse effects. Accurate measurement of temperature inside lithiumion batteries and understanding the temperature effects are important for the proper battery management. In this review, we discuss the effects of temperature to lithium-ion batteries at both low and high temperature ranges.The current approaches in monitoring the internal temperature of lithium-ion batteries via both contact and contactless processes are also discussed in the review. | Shuai Ma Modi Jiang Peng Tao Chengyi Song Jianbo Wu Jun Wang Tao Deng Wen Shang | 2018 | Progress in Natural Science:Materials International2018，28，6： | 27 |
| 2 | Bone biomaterials and interactions with stem cells显示文摘Bone biomaterials play a vital role in bone repair by providing the necessary substrate for cell adhesion,proliferation, and differentiation and by modulating cell activity and function. In past decades, extensive efforts have been devoted to developing bone biomaterials with a focus on the following issues:(1)developing ideal biomaterials with a combination of suitable biological and mechanical properties;(2)constructing a cell microenvironment with pores ranging in size from nanoscale to submicro-and microscale;and(3) inducing the oriented differentiation of stem cells for artificial-to-biological transformation. Here we present a comprehensive review of the state of the art of bone biomaterials and their interactions with stem cells. Typical bone biomaterials that have been developed, including bioactive ceramics, biodegradable polymers, and biodegradable metals, are reviewed, with an emphasis on their characteristics and applications. The necessary porous structure of bone biomaterials for the cell microenvironment is discussed,along with the corresponding fabrication methods. Additionally, the promising seed stem cells for bone repair are summarized, and their interaction mechanisms with bone biomaterials are discussed in detail.Special attention has been paid to the signaling pathways involved in the focal adhesion and osteogenic differentiation of stem cells on bone biomaterials. Finally, achievements regarding bone biomaterials are summarized, and future research directions are proposed. | Chengde Gao Shuping Peng Pei Feng Cijun Shuai | 2017 | Bone Research2017，5，4： | 17 |
| 3 | Clinicopathological Characteristics and Prognosis of Hepatoid Adenocarcinoma of the Stomach:Evaluation of a Pooled Case Series显示文摘Hepatoid adenocarcinoma of the stomach (HAS)is an extremely rare and unique gastric malignancy.The present study aimed to examine the relevance of the clinicopathological characteristics of HAS with patient prognosis.We retrospectively reviewed clinical data of 34 HAS patients treated at our institution between January 2010 and December 2016,as well as 294 cases reported prior to 2017 in research databases.Among these patients,45.6%(115/252)had lesions in the gastric antrum and 77.0%(235/305)were male.Elevated levels of serum alpha-fetoprotein (AFP)were detected in most patients(75/93,80.6%).Vascular invasion(199/286,69.6%),lymph node metastasis (222/283,78.4%),and preoperative distant metastasis (121/328,36.9%)were commonly observed.The 5-year disease-free survival (DFS)and disease-specific survival (DSS) were 20.7%and 29.2%,respectively.DFS and DSS of patients receiving neoadjuvant therapy were significantly higher than those of patients receiving postoperative adjuvant therapy [DFS:P<0.001, hazard ratio (HR)=-1.831,95%confidence interval (CI):0.060-0.429;DSS:P<0.001,HR=-2.185, 95%CI:0.032-0.401].In conclusion,HAS exhibits distinct clinicopathological characteristics and a strikingly worse prognosis when compared with common gastric cancer.Complete surgery,early pTNM stage,and adjuvant therapy may predict a more favorable prognosis.Neoadjuvant therapy is strongly recommended for patients with lymph node metastasis or/and preoperative distant metastasis. | Xiang-yu ZENG Yu-ping YIN Hua XIAO Peng ZHANG Jun HE Wei-zhen LIU Jin-bo GAO Xiao-ming SHUAI Guo-bin WANG Xiu-li WU Kai-xiong TAO | 2018 | Current Medical Science2018，38，6： | 17 |
| 4 | First mass measurement of short-lived nuclides at HIRFL-CSR显示文摘Recent commissioning of the HIRFL-CSR has demonstrated its ability to perform direct mass measurement for short-lived nuclides. Projectile fragments produced by the 78Kr ions at 481.88 MeV/u were separated with the new radioactive beam line in Lanzhou (RIBLL2), and injected into and stored in the experimental storage ring (CSRe). By operating the CSRe as an isochronous mass spectrometry, a typical mass resolution around 2.0×10?5 has been achieved. The masses for 63Ge, 65As and 67Se were measured for the first time. The measured masses are compared with theoretical predictions and the location of the proton drip-line for As isotopes is discussed. The implication of the 65As mass in the astrophysical rapid proton capture process has also been addressed. | XU HuShan TU XiaoLin YUAN YouJin XIA JiaWen ZANG YongDong WANG Meng MAO RuiShi HU ZhengGuo MEI Bo SHUAI Peng ZHU ZhiChao TANG ShuWen GENG Peng LIU Yong ZHAO TieCheng ZHANG XueYing YANG JianCheng MAO LiJun ZHANG YuHu ZHOU XiaoHong HUANG WenXue XIAO GuoQing ZHAO HongWei ZHAN WenLong | 2009 | Chinese Science Bulletin2009，54，24： | 11 |
| 5 | Role of macrophages in peripheral nerve injury and repair显示文摘Resident and inflammatory macrophages are essential effectors of the innate immune system. These cells provide innate immune defenses and regulate tissue and organ homeostasis. In addition to their roles in diseases such as cancer, obesity and osteoarthritis, they play vital roles in tissue repair and disease rehabilitation. Macrophages and other inflammatory cells are recruited to tissue injury sites where they promote changes in the microenvironment. Among the inflammatory cell types, only macrophages have both pro-inflammatory (Ml) and anti-inflammatory (M2) actions, and M2 macrophages have four subtypes. The co-action of Ml and M2 subtypes can create a favorable microenvironment, releasing cytokines for damaged tissue repair. In this review, we discuss the activation of macrophages and their roles in severe peripheral nerve injury. We also describe the therapeutic potential of macrophages in nerve tissue engineering treatment and highlight approaches for enhancing M2 cell-mediated nerve repair and regeneration. | Ping Liu Jiang Peng Gong-Hai Han Xiao Ding Shuai Wei Gang Gao Kun Huang Feng Chang Yu Wang | 2019 | Neural Regeneration Research2019，14，8： | 11 |
| 6 | Aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells显示文摘 | Shuai Fang Chao Wang Jian Xiang Liang Cheng Xuejiao Song Ligeng Xu Rui Peng Zhuang Liu | 2014 | Nano Research2014，7，9： | 10 |
| 7 | Diffusion bonding of AlCoCrFeNi2.1 eutectic high entropy alloy to TiAl alloy显示文摘High entropy alloys have special microstructure and remarkable properties.To explore their potential engineering application in high temperature structures,the microstructure evolution of bonding interface,the elemental diffusion behavior and mechanical property of the diffusion bonded joint between AlCoCrFeNi2.1eutectic high entropy alloy(EHEA)and TiAl alloy were investigated.Four reaction layers(rodlike B2 phase,Al(Co,Ni)2Ti,τ3-Al3NiTi2+TiAl,τ3-Al3NiTi2+TiAl+Ti3Al)formed in the diffusion zone near FCC phase of EHEA,but three layers(Al(Co,Ni)2Ti,τ3-Al3Ni Ti2+Ti Al,τ3-Al3Ni Ti2+Ti Al+Ti3Al)formed near B2 phase.Al and Ni controlled the reaction diffusion of EHEA and TiAl alloy,coarsened the acicular precipitated B2 phase and turned Ti Al phase into Al(Co,Ni)2Ti andτ3-Al3NiTi2 phases.All these reaction layers grew in a parabolic manner as a function of bonding temperature.Rodlike B2 phase has the lowest growth activation energy of 125.2 kJ/mol,and the growth activation energy ofτ3-Al3Ni Ti2+TiAl layer near B2 phase is much lower than that near FCC phase.The penetration phenomenon and convex structure formed in the diffusion zone,which resulted in interlocking effect and enhanced the strength of resultant joints.The highest shear strength of 449 MPa was achieved at 950℃.And the brittle fracture generally initiated at the interface between Al(Co,Ni)2Ti andτ3-Al3NiTi2+TiAl layers. | Peng Li Shuai Wang Yueqing Xia Xiaohu Hao Honggang Dong | 2020 | Journal of Materials Science & Technology2020，42，10： | 8 |
| 8 | Improved Relative-transformation Principal Component Analysis Based on Mahalanobis Distance and Its Application for Fault Detection显示文摘主要部件分析(PCA ) 广泛地在过程工业被使用了,它能维持最大的差错察觉率。尽管许多问题在 PCA 被处理了,一些必要问题仍然保持未解决。这研究以下列方法为差错察觉性能改进 PCA。第一,一个相对转变计划基于 Mahalanobis 距离(MD ) 被介绍消除数据的尺寸的效果而不是无尺寸的标准化,并且改进精确性和差错察觉的即时性能。理论推导证明那相对转变能直接基于 MD 消除尺寸的效果并且在结果显示出的相对空间,分析和模拟给 PCA 的合理解释它的优势和有效性。第二,一个改进摆平的预言错误(SPE ) 统计数值被给改进标准化 PCA 的差错察觉表演,它能使标准化基于 PCA 的差错察觉方法成为对实际工业过程合适的更多。最后,二个改进方法被联合更有效地检测差错。建议方法被使用在热连续滚动过程检测 looper 系统的单个差错和多差错,模拟结果以易感知,精确性和差错察觉的即时性能为差错察觉性能表明这些改进的有效性。 | SHI Huai-Tao LIU Jian-Chang XUE Peng ZHANG Ke WU Yu-Hou ZHANG Li-Xiu TAN Shuai | 2013 | 自动化学报2013，39，9： | 8 |
| 9 | Accelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity显示文摘The incorporation of hydroxyapatite(HAP)into poly-L-lactic acid(PLLA)matrix serving as bone scaffold is expected to exhibit bioactivity and osteoconductivity to those of the living bone.While too low degradation rate of HAP/PLLA scaffold hinders the activity because the embedded HAP in the PLLA matrix is difficult to contact and exchange ions with body fluid.In this study,biodegradable polymer poly(glycolic acid)(PGA)was blended into the HAP/PLLA scaffold fabricated by laser 3D printing to accelerate the degradation.The results indicated that the incorporation of PGA enhanced the degradation rate of scaffold as indicated by the weight loss increasing from 3.3%to 25.0%after immersion for 28 days,owing to the degradation of high hydrophilic PGA and the subsequent accelerated hydrolysis of PLLA chains.Moreover,a lot of pores produced by the degradation of the scaffold promoted the exposure of HAP from the matrix,which not only activated the deposition of bone like apatite on scaffold but also accelerated apatite growth.Cytocompatibility tests exhibited a good osteoblast adhesion,spreading and proliferation,suggesting the scaffold provided a suitable environment for cell cultivation.Furthermore,the scaffold displayed excellent bone defect repair capacity with the formation of abundant new bone tissue and blood vessel tissue,and both ends of defect region were bridged after 8 weeks of implantation. | Cijun Shuai Wenjing Yang Pei Feng Shuping Peng Hao Pan | 2021 | Bioactive Materials2021，6，2： | 8 |
| 10 | Frequency stabilization and tuning of breathing solitons in Si3N4 microresonators显示文摘Dissipative Kerr solitons offer broadband coherent and low-noise frequency combs and stable temporal pulse trains,having shown great potential applications in spectroscopy,communications,and metrology.Breathing solitons are a particular kind of dissipative Kerr soliton in which the pulse duration and peak intensity show periodic oscillation.Here we have investigated the breathing dissipative Kerr solitons in silicon nitride(Si3N4)microrings,while the breathing period shows uncertainties of around megahertz(MHz)order in both simulation and experiments.This instability is the main obstacle for future applications.By applying a modulated signal to the pump laser,the breathing frequency can be injection locked to the modulation frequency and tuned over tens of MHz with frequency noise significantly suppressed.Our demonstration offers an alternative knob for the control of soliton dynamics in microresonators and paves a new avenue towards practical applications of breathing solitons. | SHUAI WAN RUI NIU ZHENG-YU WANG JIN-LAN PENG MING LI JIN LI GUANG-CAN GUO CHANG-LING ZOU CHUN-HUA DONG | 2020 | Photonics Research2020，8，8： | 8 |
| 11 | Protective effects of Bushen Tiansui decoction on hippocampal synapses in a rat model of Alzheimer's disease显示文摘Bushen Tiansui decoction is composed of six traditional Chinese medicines:Herba Epimedii,Radix Polygoni multiflori,Plastrum testudinis,Fossilia Ossis Mastodi,Radix Polygalae,and Rhizoma Acorus tatarinowii.Because Bushen Tiansui decoction is effective against amyloid beta(Aβ) toxicity,we hypothesized that it would reduce hippocampal synaptic damage and improve cognitive function in Alzheimer's disease.To test this hypothesis,we used a previously established animal model of Alzheimer's disease,that is,microinjection of aggregated Aβ25–35 into the bilateral brain ventricles of Sprague-Dawley rats.We found that long-term(28 days) oral administration of Bushen Tiansui decoction(0.563,1.688,and 3.375 g/m L;4 m L/day) prevented synaptic loss in the hippocampus and increased the expression levels of synaptic proteins,including postsynaptic density protein 95,the N-methyl-D-aspartate receptor 2 B subunit,and Shank1.These results suggested that Bushen Tiansui decoction can protect synapses by maintaining the expression of these synaptic proteins.Bushen Tiansui decoction also ameliorated measures reflecting spatial learning and memory deficits that were observed in the Morris water maze(i.e.,increased the number of platform crossings and the amount of time spent in the target quadrant and decreased escape latency) following intraventricular injections of aggregated Aβ25–35 compared with those measures in untreated Aβ_(25–35)-injected rats.Overall,these results provided evidence that further studies on the prevention and treatment of dementia with this traditional Chinese medicine are warranted. | Shan Hui Yu Yang Wei-jun Peng Chen-xia Sheng Wei Gong Shuai Chen Pan-pan Xu Zhe Wang | 2017 | Neural Regeneration Research2017，12，10： | 7 |
| 12 | Investigation of CFD Calculation Method of a Centrifugal Pump with Unshrouded Impeller显示文摘Currently, relatively large errors are found in numerical results in some low-specific-speed centrifugal pumps with unshrouded impeller because the effect of clearances and holes are not accurately modeled. Establishing an accurate analytical model to improve performance prediction accuracy is therefore necessary. In this paper, a three-dimensional numerical simulation is conducted to predict the performance of a low-specific-speed centrifugal pump, and the modeling, numerical scheme, and turbulent selection methods are discussed. The pump performance is tested in a model pump test bench, and flow rate, head, power and efficiency of the pump are obtained. The effect of taking into consideration the back-out vane passage, clearance, and balance holes is analyzed by comparing it with experimental results, and the performance prediction methods are validated by experiments. The analysis results show that the pump performance can be accurately predicted by the improved method. Ignoring the back-out vane passage in the calculation model of unshrouded impeller is found to generate better numerical results. Further, the calculation model with the clearances and balance holes can obviously enhance the numerical accuracy. The application of disconnect interface can reduce meshing difficulty but increase the calculation error at the off-design operating point at the same time. Compared with the standard k–ε, renormalization group k–ε, and Spalart–Allmars models, the Realizable k–ε model demonstrates the fastest convergent speed and the highest precision for the unshrouded impeller flow simulation. The proposed modeling and numerical simulation methods can improve the performance prediction accuracy of the low-specific-speed centrifugal pumps, and the modeling method is especially suitable for the centrifugal pump with unshrouded impeller. | WU Dazhuan YANG Shuai XU Binjie LIU Qiaoling WU Peng WANG Leqin | 2014 | Chinese Journal of Mechanical Engineering2014，27，2： | 7 |
| 13 | Dynamic Modeling and Analysis of 5‑PSS/UPU Parallel Mechanism with Elastically Active Branched Chains显示文摘To study the characteristics of the 5-prismatic–spherical–spherical(PSS)/universal–prismatic–universal(UPU)parallel mechanism with elastically active branched chains,the dynamics modeling and solutions of the parallel mechanism were investigated.First,the active branched chains and screw sliders were considered as spatial beam elements and plane beam element models,respectively,and the dynamic equations of each element model were derived using the Lagrange method.Second,the equations of the 5-PSS/UPU parallel mechanism were obtained according to the kinematic coupling relationship between the active branched chains and moving platform.Finally,based on the parallel mechanism dynamic equations,the natural frequency distribution of the 5-PSS/UPU parallel mechanism in the working space and elastic displacement of the moving platform were obtained.The results show that the natural frequency of the 5-PSS/UPU parallel mechanism under a given motion situation is greater than its operating frequency.The maximum position error is -0.096 mm in direction Y,and the maximum orientation error is -0.29°around the X-axis.The study provides important information for analyzing the dynamic performance,dynamic optimization design,and dynamic control of the 5-PSS/UPU parallel mechanism with elastically active branched chains. | Yanbiao Li Hang Zheng Bo Chen Peng Sun Zesheng Wang Kun Shuai Yi Yue | 2020 | Chinese Journal of Mechanical Engineering2020，33，3： | 7 |
| 14 | Promoting-mode free formalism for excited state radiationless decay process with Duschinsky rotation effect显示文摘In the present work, through the path integral of Gaussian type correlation function, a new formalism based on Fermi-Golden Rule for calculating the rate constant of nonradiative decay process with Duschinsky rotation effect in polyatomic molecules is developed. The advantage of the present path-integral formalism is promoting-mode free. In order to get the rate constant, a 'transition rate matrix' needs to be calculated. The rate constant calculated previously is only an approximation of diagonal elements of our 'transition rate matrix ' . The total rate should be the summation over all the matrix elements. | NIU YingLi PENG Qian SHUAI ZhiGang | 2008 | Science China Chemistry2008，51，12： | 6 |
| 15 | Therapeutic strategies for peripheral nerve injury: decellularized nerve conduits and Schwann cell transplantation显示文摘In recent years, the use of Schwann cell transplantation to repair peripheral nerve injury has attracted much attention. Animal-based studies show that the transplantation of Schwann cells in combination with nerve scaffolds promotes the repair of injured peripheral nerves. Autologous Schwann cell transplantation in humans has been reported recently. This article reviews current methods for removing the extracellular matrix and analyzes its composition and function. The development and secretory products of Schwann cells are also reviewed. The methods for the repair of peripheral nerve injuries that use myelin and Schwann cell transplantation are assessed. This survey of the literature data shows that using a decellularized nerve conduit combined with Schwann cells represents an effective strategy for the treatment of peripheral nerve injury. This analysis provides a comprehensive basis on which to make clinical decisions for the repair of peripheral nerve injury. | Gong-Hai Han Jiang Peng Ping Liu Xiao Ding Shuai Wei Sheng Lu Yu Wang | 2019 | Neural Regeneration Research2019，14，8： | 6 |
| 16 | Feasibility and nutritional impact of laparoscopic assisted tailored subtotal gastrectomy for middle-third gastric cancer显示文摘BACKGROUND Laparoscopic assisted total gastrectomy(LaTG)is associated with reduced nutritional status,and the procedure is not easily carried out without extensive expertise.A small remnant stomach after near-total gastrectomy confers no significant nutritional benefits over total gastrectomy.In this study,we developed a modified laparoscopic subtotal gastrectomy procedure,termed laparoscopicassisted tailored subtotal gastrectomy(LaTSG).AIM To evaluate the feasibility and nutritional impact of LaTSG compared to those of LaTG in patients with advanced middle-third gastric cancer(GC).METHODS We retrospectively analyzed surgical and oncological outcomes and postoperative nutritional status in 92 consecutive patients with middle-third GC who underwent radical laparoscopic gastrectomy at Department of Pancreatic Stomach Surgery,National Cancer Center/Cancer Hospital,Chinese Academy of Medical Sciences,and Peking Union Medical College between 2013 and 2017.Of these 92 patients,47 underwent LaTSG(LaTSG group),and the remaining underwent LaTG(LaTG group).RESULTS Operation time(210±49.8 min vs 208±50.0 min,P>0.05)and intraoperative blood loss(152.3±166.1 mL vs 188.9±167.8 mL,P>0.05)were similar between the groups.The incidence of postoperative morbidities was lower in the LaTSG group than in the LaTG group(4.2%vs 17.8%,P<0.05).Postoperatively,nutritional indices did not significantly differ,until postoperative 12 mo.Albumin,prealbumin,total protein,hemoglobin levels,and red blood cell counts were significantly higher in the LaTSG group than in the LaTG group(P<0.05).No significant differences in Fe or C-reaction protein levels were found between the two groups.Endoscopic examination demonstrated that reflux oesophagitis was more common in the LaTG group(0%vs 11.1%,P<0.05).Kaplan–Meier analysis showed a significant improvement in the overall survival(OS)and disease free survival(DFS)in the LaTSG group.Multivariate analysis showed that LaTSG was an independent prognostic factor for OS(P=0.048)but not for DFS(P=0.054).Subgroup analysis showed that compared to LaTG,LaTSG improved the survival of patients with stage III cancers,but not for other stages.CONCLUSION For advanced GC involving the middle third stomach,LaTSG can be a good option with reduced morbidity and favorable nutritional status and oncological outcomes. | Hao Liu Peng Jin Fu-Hai Ma Shuai Ma Yi-Bin Xie Yang Li Wei-Kun Li Wen-Zhe Kang Yan-Tao Tian | 2020 | World Journal of Gastroenterology2020，26，43： | 6 |
| 17 | Characterization of myelin oligodendrocyte glycoprotein (MOG)35–55- specific CD8^+ T cells in experimental autoimmune encephalomyelitis显示文摘Background:The pathogenesis of multiple sclerosis(MS)is mediated primarily by T cells,but most studies of MS and its animal model,experimental autoimmune encephalomyelitis(EAE),have focused on CD4^+ T cells.The aims of the current study were to determine the pathological interrelationship between CD4 and CD8 autoreactive T cells in MS/EAE.Methods:Female C57BL/6 mice(n=20)were induced by myelin oligodendrocyte glycoprotein(MOG)35-55 peptide.At 14 days after immunization,T cells were isolated from the spleen and purified as CD4^+ and CD8^+ T cells by using CD4 and CD8 isolation kits,and then the purity was determined by flow cytometric analysis.These cells were stimulated by MOG35–55 peptide and applied to proliferation assays.The interferon-gamma(IFN-γ)and interleukin(IL)-4 secretion of supernatant of cultured CD4^+ and CD8^+ T cells were measured by enzyme-linked immunosorbent assays(ELISA).For adoptive transfer,recipient mice were injected with MOG35–55-specific CD8^+ or CD4^+ T cells.EAE clinical course was measured by EAE score at 0–5 scale and spinal cord was examined by staining with hematoxylin and eosin and Luxol fast blue staining.Results:CD8^+ CD3^+ and CD4^+ CD3^+ cells were 86%and 94%pure of total CD3^+ cells after CD8/CD4 bead enrichment,respectively.These cells were stimulated by MOG35–55 peptide and applied to proliferation assays.Although the CD8^+ T cells had a generally lower response to MOG35–55 than CD4^+ T cells,the response of CD8^+ T cells was not always dependent on CD4.CD8^+ T cell secreted less IFN-γand IL-4 compared with CD4^+ T cells.EAE was induced in wildtype B6 na?ve mice by adoptive transfer of MOG35–55-specific T cells from B6 active-induced EAE(aEAE)mice.A similar EAE score and slight inflammation and demyelination were found in naive B6 mice after transferring of CD8^+ T cells from immunized B6 mice compared with transfer of CD4^+ T cells.Conclusion:Our data suggest that CD8^+ autoreactive T cells in EAE have a lower encephalitogenic function but are unique and independent on pathogenic of EAE rather than their CD4^+ counterparts. | Yong Peng Fei-Zhou Zhu Zhi-Xing Chen Jian-Xiong Zhou Lu Gan Shan-Shan Yang Shuai Gao Qian-Qian Liu | 2019 | Chinese Medical Journal2019，，24： | 5 |
| 18 | Incidence and clinical characteristics of inflammatory bowel disease in a developed region of G uangdong P rovince, C hina: A prospective population‐based study显示文摘 | Zhirong Zeng Zhenhua Zhu Yuyu Yang Weishan Ruan Xiabiao Peng Yuhuan Su Lin Peng Jinquan Chen Quan Yin Chao Zhao Haihua Zhou Shuai Yuan Yuantao Hao Jiaming Qian Siew Chien Ng Minhu Chen Pinjin Hu | 2013 | J Gastroenterol Hepatol2013，，7： | 5 |
| 19 | Distinct regulatory mechanism of immunoglobulin gene transcription in epithelial cancer cells显示文摘The restriction of immunoglobulin(Ig)expression to B lymphocytes is well established.However,several reports have confirmed that the Ig gene can be expressed in many non-B cancer cells and/or some normal cells.Our aim is to determine whether the Ig gene promoter can be activated in non-B cancer cells and to identify the regulatory mechanism for Ig gene expression.Our results show that the Ig promoter of VH4-59 was activated in several non-B cancer cell lines.Moreover,two novel positive regulatory elements,an enhancer-like element at 2800 to 2610 bp and a copromoter-like element at 2610 to 2300 bp,were identified in two epithelial cancer cell lines,HeLa S3 and HT-29.The octamer element(59-ATGCAAAT-39)located in the Ig promoter,a crucial element for B-cell-derived Ig gene transcription,was also very important for non-B-cell-derived Ig gene transcription.More importantly,we confirmed that octamer-related protein-1(Oct-1),but not Oct-2,was a crucial transcriptional factor for Ig gene transcription due to its ability to bind to the octamer element of the Ig promoter in epithelial cancer cells.These results suggested the presence of a distinct regulatory mechanism for Ig gene expression in non-B cancer cells. | Xiaohui Zhu Lina Wu Li Zhang Peng Hao Shuai Zhang Jing Huang Jie Zheng Yinan Liu Wenjun Li Yingmei Zhang Chunyan Zhou Youhui Zhang C Cameron Yin Xiaoyan Qiu | 2010 | Cellular & Molecular Immunology2010，7，4： | 5 |
| 20 | LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells显示文摘LIN28A,an RNA-binding protein,plays an important role in porcine induced pluripotent stem cells(piPSCs).However,the molecular mechanism underlying the function of LIN28A in the maintenance of pluripotency in piPSCs remains unclear.Here,we explored the function of LIN28A in piPSCs based on its overexpression and knockdown.We performed total RNA sequencing(RNA-seq)of piPSCs and detected the expression levels of relevant genes by quantitative real-time polymerase chain reaction(qRT-PCR),western blot analysis,and immunofluorescence staining.Results indicated that piPSC proliferation ability decreased following LIN28A knockdown.Furthermore,when LIN28A expression in the shLIN28A2 group was lower(by 20%)than that in the negative control knockdown group(shNC),the pluripotency of piPSCs disappeared and they differentiated into neuroectoderm cells.Results also showed that LIN28A overexpression inhibited the expression of DUSP(dual-specificity phosphatases)family phosphatases and activated the mitogen-activated protein kinase(MAPK)signaling pathway.Thus,LIN28A appears to activate the MAPK signaling pathway to maintain the pluripotency and proliferation ability of piPSCs.Our study provides a new resource for exploring the functions of LIN28A in piPSCs. | Xiao-Long Wu Zhen-Shuo Zhu Xia Xiao Zhe Zhou Shuai Yu Qiao-Yan Shen Ju-Qing Zhang Wei Yue Rui Zhang Xin He Sha Peng Shi-Qiang Zhang Na Li Ming-Zhi Liao Jin-Lian Hua | 2021 | Zoological Research2021，42，3： | 5 |
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