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| 1 | Isolation of the promoter of a cotton β-galactosidase gene (GhGal1) and its expression in transgenic tobacco plants显示文摘β-galactosidases (EC 3.2.1.23) constitute a widespread family of glycosyl hydrolases in plants and are thought to be involved in metabolism of cell wall polysaccharides. A cDNA of the cotton (Gossypium hirsutum) β-galactosidase gene, designated GhGal1, has previously been identified and its transcripts are highly abundant at the elongation stage of the cotton fiber. To examine the temporal and spatial control of GhGal1 expression, a transcriptional fusion of the GhGal1 promoter region (1770 bp) with the β-glucuronidase (GUS) reporter gene was introduced into tobacco plants by the Agrobacterium infection method. The resulting transgenic plants showed higher GUS activity of fruit in the transgenic plants than that in the negative and positive controls. Histochemical localization of GUS activity demonstrated that the expression of the GUS gene could be found in the meristem zones of roots, cotyledons, vascular tissues, fruit and trichomes in transgenic tobacco plants. Additionally, se-quence analysis of the regulatory region also revealed several conserved motifs among which some were shared with previously reported fruit/seed-specific elements and the others were related with trichome expression. These results indicated the temporal and spatial expression characterization of the GhGal1 promoter in transgenic tobacco plants and provided an important insight into the roles of GhGal1 in cotton fiber development. | WU Aimin LIU Jinyuan | 2006 | Science China(Life Sciences)2006，49，2： | 5 |
| 2 | SNORD126 promotes HCC and CRC cell growth by activating the PI3K-AKT pathway through FGFR2显示文摘小核仁的 RNA (snoRNA ) 机能障碍与癌症开发被联系了。SNORD126 是在它的主机基因的 introns 5-6 以内被编码的孤儿 C/D 框 snoRNA, cyclin 交往 B1 蛋白质 1 (CCNB1IP1 ) 。SNORD126 触发的联系癌症的分子的机制充分没被理解。这里,我们证明 SNORD126 高度在 hepatocellular 癌(HCC ) 被表示, colorectal 癌症(CRC ) 病人取样。SNORD126 在裸体老鼠增加了 Huh-7 和 SW480 细胞生长和 tumorigenicity。SNORD126 击倒禁止的 HepG2 和 LS174T 房间生长。我们证实 SNORD126 没与 miRNA 活动被处理成小 RNA。而且, SNORD126 没在 HCC 样品与 CCNB1IP1 显示出重要表示关联或调整 CCNB1IP1 表示。我们的基因表示侧面分析显示了基因经常印射到 PI3K-AKT 小径的那 SNORD126-upregulated。SNORD126 overexpression 增加了 phosphorylated AKT, GSK-3,和 p70S6K 的层次并且提高了成纤维细胞生长因素受体 2 (FGFR2 ) 表示。在 SNORD126-overexpressing Huh-7 细胞的 FGFR2 的调停 siRNA 的击倒或调停 AZD4547 的 inactivation 禁止了 AKT phosphorylation 并且压制了细胞生长。这些调查结果在癌症为 SNORD126 显示一个 oncogenic 角色并且作为一个治疗学的目标建议它的潜力。 | Xianlong Fang Dongmei Yang Hongping Luo Shuai Wu Wenjie Dong Jing Xiao Sujing Yuan Aimin Ni Kang-Jian Zhang Xin-Yuan Liu Liang Chu | 2017 | Journal of Molecular Cell Biology2017，9，3： | 5 |
| 3 | The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii显示文摘Bamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value.Unique among traditional flowering plants,many bamboo plants undergo extensive synchronized flowering followed by large-scale death,seriously affecting the productivity and application of bamboo forests.To date,the molecular mechanism of bamboo flowering characteristics has remained unknown.In this study,a SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1(SOC1)-like gene,BoMADS50,was identified from Bambusa oldhamii.BoMADS50 was highly expressed in mature leaves and the floral primordium formation period during B.oldhamii flowering and overexpression of BoMADS50 caused early fl owering in transgenic rice.Moreover,BoMADS50 could interact with APETALA1/FRUITFULL(AP1/FUL)-like proteins(BoMADS 14-1/2,BoMADS15-1/2)in vivo,and the expression of BoMADS50 was signi ficantly promoted by BoMADS14-1,further indicating a synergistic effect between BoMADS50 and BoAP1/FUL-like proteins in regulating B.oldhamii flowering.We also identi fi ed four additional transcripts of BoMADS50(BoMADS50-1/2/3/4)with different nucleotide variations.Although the protein-CDS were polymorphic,they had flowering activation functions similar to those of BoMADS50.Yeast one-hybrid and transient expression assays subsequently showed that both BoMADS50 and BoMADS50-1 bind to the promoter fragment of itself and the SHORT VEGETATIVE PHASE(SVP)-like gene BoSVP,but only BoMADS50-1 can positively induce their transcription.Therefore,nucleotide variations likely endow BoMADS50-1 with strong regulatory activity.Thus,BoMADS50 and BoMADS50-1/2/3/4 are probably important positive flowering regulators in B.oldhamii.Moreover,the functional conservatism and speci ficity of BoMADS50 and BoMADS50-1 might be related to the synchronized and sporadic flowering characteristics of B.oldhamii. | Dan Hou Ling Li Tengfei Ma Jialong Pei Zhongyu Zhao Mengzhu Lu Aimin Wu Xinchun Lin | 2021 | Horticulture Research2021，8，1： | 4 |
| 4 | Influence of boron contents on properties of AlMgB films prepared by RF magnetron sputtering显示文摘Ternary AlMgB thin films were synthesized on silicon (100) substrate at 573 K by radio frequency (RF) magnetron sputtering method using one Al/Mg co-target and one boron target. The thickness of the as-deposited thin film was controlled to 500 nm by adjusting deposition time. The influences of sputtering powers on the elemental contents and structural and mechanical properties were investigated by electron probe microanalysis (EPMA), X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), and nanoindentation system. At the same time, the ball-on-disk tribometer was used to measure the friction behavior of the films. Experimental results indicate that the as-deposited boron-rich films are primarily amorphous structure and possess a dramatic high hardness up to 39 GPa with 99.03 at.% boron. Obviously, it has exceeded the hardness value of 32 GPa of pure AlMgB 14 bulk material prepared by sintering method. Furthermore, the friction coefficients of the thin films exhibit an average value as low as 0.3, which is considered as the effect of self-lubricating. | Qu, Wenchao Wu, Aimin Wu, Zhanling Bai, Yizhen Jiang, Xin | 2012 | Rare Metals2012，31，2： | 3 |
| 5 | Fabrication and its characteristics of hard coating Ti-Al-N system prepared by DC magnetron sputtering显示文摘Pseudobinary Ti 1 x Al x N films were synthesized on Si (100) wafer by DC magnetron sputtering method using Ti 1 x Al x alloy targets with different Al contents. The composition of the Ti 1 x Al x N films was determined by electron probe microanalysis (EPMA). Structural characteristic was performed by X-ray diffraction (XRD), transmission electron microscopy (TEM), and high-resolution TEM (HRTEM). First principles virtual crystal calculations for the Ti 1 x Al x N disordered alloys were used for the XRD simulations. The crystalline structure of the Ti 0.61 Al 0.39 N film was found to be a metastable single phase with NaCl (B1) structure. Its lattice constant, determined by XRD, was less than that of pure TiN. With the increase of Al content, the lattice constant of B1 phase was continually decreased, while würtzite (B4) structure was observed in the Ti 0.40 Al 0.60 N film. When x reached 0.75, the B1 phase disappeared, and only B4 phase was remained. The critical Al content for the phase transition from NaCl to würtzite structure in this paper was about 0.60, which could be explained by both the thermodynamic model and the electron theory. As-deposited Ti 1 x Al x N films exhibited excellent mechanical properties. Hardness measurements of Ti 1 x Al x N films showed a high value of 45GPa for x=0.39 and was decreased to value of 27 GPa with increasing Al at x=0.60. | Zhou, Xuyang Wu, Aimin Qu, Wenchao Jiang, Xin | 2012 | Rare Metals2012，31，2： | 3 |
| 6 | Human hepatopoietin augmenter of liver regeneration──A hepatotrophic factor for liver regeneration, and its potential antihepatitis effect in vivo显示文摘The effect of human hapatopoietin i.e. augmenter of liver regeneration (hALR) was determined on hepatocyte DNA synthesis, and on CCl 4-induced hepatitis in animal in vitro and in vivo. It is found that ALR could directly stimulate DNA synthesis of hepatocytes in primary culture in a dose-dependent manner. In 30% hepatectomied rats, significant DNA synthesis occurred in control rats ; even so, exogenous hALR gene encoding protein increased DNA synthesis of regeneration liver by 2.3 fold compared with control rats. A lower dose of CCl 4 was administrated in rats and the effect of ALR on DNA synthesis of regenerating liver 48 h after CCl 4 administration was analyzed. Few Budr-labeled hepatocytes were visible in control rats. However, exogenous hALR markedly increased the number of labeled cells in a dose-dependent manner. Statistical analysis showed that 10 or 40 μg·kg -1 ALR protein stimulated DNA synthesis by 1.7 and 4.8 fold respectively. In addition to enhancing cell growth, adiministration of hALR achieved a significant improvement in reversing the lethality of rat hepatic failure when compared with that of control group; the elevation of cytosolic enzymes was dramatically suppressed by exogenous hALR in CCl 4-treated mice in vivo and in vitro; histologically, hepatocytes around the central vein were necrotic, and the degree of hepatocyte necrosis in control mice was more prominent than that in the mice given 40 μg·kg-1 hALR 48 h after CCl 4 administration. We also noted that hALR had a strong antihepatitis effect in vitro which was determined with primary cultured rat hepatocytes. These findings suggest that hALR protects the integrity of hepatocytes against severe hepatitis, and indicate that ALR may be an important regulator of liver regeneration and play a major role in liver injury repair. It proves ALR adminstration to be a useful treatment to accelerate liver regeneration and to prevent the onset of hepatitis or intrahepatic cholestasis induced by toxin. | YANG Xiaoming 1, WANG Aimin 2, ZHOU Ping 3, XIE Ling 1, WANG Qingming 1, WU Zuze 1 and HE Fuchu 1 1. Institute of Radiation Medicine, Beijing 100850, China 2. No. 252 Hospital of PLA, Baoding 071000, China 3. General Hospital of the Air | 1998 | Chinese Science Bulletin1998，43，12： | 3 |
| 7 | Methylesterification of cell-wall pectin controls the diurnal flower-opening times in rice显示文摘Flowers are the core reproductive organ of plants, and flowering is essential for cross-pollination. Diurnal flower-opening time is thus a key trait influencing reproductive isolation, hybrid breeding, and thermostability in plants. However, the molecular mechanisms controlling this trait remain unknown. Here, we report that rice Diurnal Flower Opening Time 1 (DFOT1) modulates pectin methylesterase (PME) activity to regulate pectin methylesterification levels of the lodicule cell walls, which affect lodicule swelling to control diurnal flower-opening time. DFOT1 is specifically expressed in the lodicules, and its expression gradually increases with the approach to flowering but decreases with flowering. Importantly, a knockout of DFOT1 showed earlier diurnal flower opening. We demonstrate that DFOT1 interacts directly with multiple PMEs to promote their activity. Knockout of PME40 also resulted in early diurnal flower opening, whereas overexpression of PME42 delayed diurnal flower opening. Lower PME activity was observed to be associated with higher levels of pectin methylesterification and the softening of cell walls in lodicules, which contribute to the absorption of water by lodicules and cause them to swell, thus promoting early diurnal flower opening. Higher PME activity had the opposite effect. Collectively, our work uncovers a molecular mechanism underlying the regulation of diurnal flower-opening time in rice, which would help reduce the costs of hybrid breeding and improve the heat tolerance of flowering plants by avoiding higher temperatures at anthesis. | Mumei Wang Xiaopei Zhu Guoqing Peng Minglong Liu Shuqing Zhang Minghao Chen Shitang Liao Xiaoying Wei Peng Xu Xiyu Tan Fangping Li Zhichuan Li Li Deng Ziliang Luo Liya Zhu Shuai Zhao Dagang Jiang Jing Li Zhenlan Liu Xianrong Xie Shaokui Wang Aimin Wu Chuxiong Zhuang Hai Zhou | 2022 | Molecular Plant2022，15，6： | 2 |
| 8 | Clay with high fluorine and endemic fluorosis caused by indoor combustion of coal in southwestern China显示文摘 | Baoshan ZHENG Daishe WU Binbin WANG Mingshi WANG Xiaojing LIU Aimin WANG Guisen XIAO Pugao LIU Robert B. Finkelman | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 2 |
| 9 | 转录组-代谢组联合分析揭示低温下采后雷竹笋木质化分子调控机制显示文摘雷竹(Phyllostachys violascens)笋是中国传统的山珍之一,是一种天然绿色食品。但其采后易消耗大量水分和营养而快速老化,在一般条件下难以贮藏。文中首次通过“转录组—代谢组”联合分析揭示了低温贮藏条件下雷竹笋木质化的调控机制,研究表明:与常温贮藏相比,低温可显著抑制采后竹笋中PAL、POD酶活性,降低失水率和总木质素含量,从而将竹笋保鲜时间至少延长20 d。通过“转录组—代谢组”联合分析表明,低温下竹笋木质素前体物质、茉莉酸含量呈上升趋势,并在后期显著富集,说明低温可诱导茉莉酸积累、抑制木质素合成。对低温响应、茉莉酸合成和木质素合成通路关键基因进行筛选,并通过共表达分析发现,低温信号可能通过“低温—木质化”和“低温—茉莉酸—木质化”2种通路共同抑制采后竹笋中木质素合成,延缓其保鲜时间。上述结果可为揭示采后竹笋木质化分子调控通路研究提供了一定依据。 | 侯丹(翻译) 林新春(翻译) Haiwen Lu Zhongyu Zhao Jialong Pei Huqing Yang Aimin Wu Xuejun Yu | 2021 | 世界竹藤通讯2021，19，5： | 2 |
| 10 | Preparation of a novel anion exchange group modified hyper-crosslinked resin for the effective adsorption of both tetracycline and humic acid显示文摘与一个阴离子交换组一起修改的新奇的超树脂(MENQ ) 经由四步作为 comonomers 用 divinylbenzene (DVB ) 和甲基 acrylate (麻省) 被准备:暂停聚合, crosslinking 以后, ammonolysis 和完化反应。获得的树脂两个都有一个高特定的表面区域(793.34 m < 啜 class= “ a-plus-plus ” > 2 | Qing ZHOU Mengqiao WANG Aimin LI Chendong SHUANG Mancheng ZHANG Xiaohan LIU Liuyan WU | 2013 | Frontiers of Environmental Science & Engineering2013，7，3： | 2 |
| 11 | Generation of transgenic cucumbers with expression of a ten-tandem repeat long-acting GLP-1 analogue and their biological function on diabetic rats显示文摘Glucagon-like peptide-1 (GLP-1) is a 30-amino acid peptide hormone, which has the regulatory function of stimulating the secretion of insulin to balance blood glucose levels. In this study, marker-free expression vector pX6 with a fusion gene of ten tandem repeated GLP-1 analog ([Ser8, Gln26 and Asp34]-GLP-1) genes was constructed and transformed into cucumbers by Agrobacterium tumefaciens-mediated transformation method. Four transgenic lines were obtained by PCR and Southern blotting analysis and two transgenic lines successfully expressed the fusion protein (named GLP-T), which was revealed by Western blotting analysis. The biological activity test results showed that the serum glucose level of diabetic rats was significantly decreased after oral administration of the fusion protein GLP-T extracted from the transgenic cucumber fruitage. These results suggest that this may provide a brand-new strategy to prevent and cure the diabetes with no pain. | ZHAO Lei LIAO Fang WANG CuiYan CHEN Miao WEI AiMin DU ShengLi MA BaiCheng HU XiaoYu WU ZhiQiang WANG RuiJu LI MingGang | 2009 | Chinese Science Bulletin2009，54，24： | 2 |
| 12 | The effect of continuous Ni(Ⅱ) exposure on the organic degradation and soluble microbial product(SMP) formation in two-phase anaerobic reactor显示文摘A two-phase anaerobic reactor fed with glucose substrate(3 g chemical oxygen demand(COD)/L) was used to investigate the effects of toxic metals on the degradation of organics and the soluble microbial product(SMP) formation. Low concentrations of Ni(II)(5 and10 mg/L) promoted the acid phase, whereas high concentrations(15, 20, and 25 mg/L)exhibited an inhibitory effect on, but did not alter the fermentative method, which mainly involved the fermentation of propionic acid. The methanogenic microorganism exhibited a strong capability adapting constantly increased Ni(II) levels. The acid phase was an accumulation stage of SMP. In the absence of Ni(II), the high-molecular-weight material in the effluent SMP mainly contained polysaccharide, tryptophan, and casein. Methanogens metabolized most of the polysaccharide, the whole tryptophan content, and part of the casein, leading to the presence of humic acid and protein in effluent. After Ni(II) dosage, the protein and polysaccharide of the acid phase increased, and tryptophan changed, while casein remained stable. More protein than polysaccharide was produced, suggesting the prominent function of protein when addressing the negative effect of toxic metals. The analysis of DNA confirmed the change of bacterial activity. | Wei Wu Tengteng Duan Hailiang Song Yan Li Ang Yu Long Zhang Aimin Li | 2015 | Journal of Environmental Sciences2015，27，7： | 2 |
| 13 | Microstructure and properties of glycerol plasticized soy protein plastics containing castor oil显示文摘 | Huafeng Tian Wenqian Wu Gaiping Guo Bagen Gaolun Qingqing Jia Aimin Xiang | 2011 | Journal of Food Engineering2011，，3： | 2 |
| 14 | Influence of polyol plasticizers on the properties of polyvinyl alcohol films fabrica- ted by melt processing显示文摘 | Wu Wenqian Tian Huafeng Xiang Aimin | 2012 | Journal of Polymers and The Envi- ronment2012，20，1： | 1 |
| 15 | Surface modification of steels and magnesium alloy by high current pulsed electron beam显示文摘 | Hao Shengzhi Gao Bo Wu Aimin | 2005 | Nuclear Instruments and Methods in Physics Research B2005，240，： | 1 |
| 16 | Adiponectin protects against acetaminophen-induced mitochondrial dysfunction and acute liver injury by promoting autophagy in mice显示文摘 | Zhuofeng Lin Fan Wu Shaoqiang Lin Xuebo Pan Leigang Jin Tingting Lu Lihua Shi Yu Wang Aimin Xu Xiaokun Li | 2014 | Journal of Hepatology2014，，： | 1 |
| 17 | Synthesis and Characterization of N-doped TiO 2 Nanowires with Visible Light Response显示文摘 | Aimin Yu Guangjun Wu Fuxiang Zhang Yali Yang Naijia Guan | 2009 | Catalysis Letters . 2009 (3-4)2009，，3： | 1 |
| 18 | Engineered high-strength biohydrogel as a multifunctional platform to deliver nucleic acid for ameliorating intervertebral disc degeneration显示文摘Intervertebral disc degeneration(IVDD)is a leading cause of low back pain.The strategy of using functional materials to deliver nucleic acids provides a powerful tool for ameliorating IVDD.However,the immunogenicity of nucleic acid vectors and the poor mechanical properties of functional materials greatly limit their effects.Herein,antagomir-204-3p(AM)shows low immunogenicity and effectively inhibits the apoptosis of nucleus pulposus cells.Moreover,a high-strength biohydrogel based on zinc-oxidized sodium alginate-gelatin(ZOG)is designed as a multifunctional nucleic acid delivery platform.ZOG loaded with AM(ZOGA)exhibits great hygroscopicity,antibacterial activity,biocompatibility,and biodegradability.Moreover,ZOGA can be cross-linked with nucleus pulposus tissue to form a high-strength collagen network that improves the mechanical properties of the intervertebral disc(IVD).In addition,ZOGA provides an advantageous microenvironment for genetic expression in which AM can play an efficient role in maintaining the metabolic balance of the extracellular matrix.The results of the radiological and histological analyses demonstrate that ZOGA restores the height of the IVD,retains moisture in the IVD,and maintains the tissue structure.The ZOGA platform shows the sustained release of nucleic acids and has the potential for application to ameliorate IVDD,opening a path for future studies related to IVD. | Tao Chen Qiuping Qian Pooyan Makvandi Ehsan Nazarzadeh Zare Qizhu Chen Linjie Chen Zhiguang Zhang Hao Zhou Wenxian Zhou Hui Wang Xiangyang Wang Yu Chen Yunlong Zhou Aimin Wu | 2023 | Bioactive Materials2023，，7： | 1 |
| 19 | Explosion of solvent vapor in a ring partition of the floating roof显示文摘 | LUO Aimin LI Wanehun WU Zongzhi | 2008 | China: Journal of Loss Prevention in the Process Industries2008，，21： | 1 |
| 20 | Reprogramming mature terminally differentiated adipocytes to induced pluripotent stem cells显示文摘Mature adipocytes are terminally differentiated somatic cells. Here, we report the successful generation of induced pluripotent stem(i PS) cells from mouse mature adipocytes by forced expression of six transcription factors(Oct4, Sox2, c-Myc, Klf4, Rarc, and Lrh1) with a piggy Bac transposon-based strategy. The resulting i PS cells were pluripotent as evidenced by the fact that they stained positive for alkaline phosphatase, expressed high levels of key pluripotency markers including Oct4, Nanog, and SSEA1,and remained pluripotent on a 2i media. In vitro differentiation of the i PS cells showed that the cell derivatives of all three germ layers could be readily obtained through formation of embryoid bodies. Most importantly, these adipocytederived i PS cells were capable of producing chimera with high frequencies when reintroduced into early-stage embryos and transmitted through the germ line. This study demonstrates that the new six-factor reprogramming technology facilitates the reset of the terminally differentiated adipocytes to the ground state of pluripotency, enabling us to fully explore the potential of mature adipocytes as a viable cell source for regenerative medicine. | Tao Nie Wei Deng Xuefei Gao Wei Sun Xiaoyan Hui Hong Song Dajiang Qin Aimin Xu Peng Li Pentao Liu Liangxue Lai Donghai Wu | 2015 | Science Bulletin2015，60，20： | 1 |
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