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| 1 | Production of bioactive ginsenoside compound K in metabolically engineered yeast显示文摘 | Xing Yan Yun Fan Wei Wei Pingping Wang Qunfang Liu Yongjun Wei Lei Zhang Guoping Zhao Jianmin Yue Zhihua Zhou | 2014 | Cell Research2014，24，6： | 81 |
| 2 | A single circular chromosome yeast显示文摘Dear editor,Most of the prokaryotic cells contain a single circular chromosome.In contrast, the eukaryotic cells usually contain multiple linear chromosomes. Recently, we artificially created a single linear chromosome yeast strain SY14 from native 16 chromosomes in a haploid Saccharomyces cerevisiae, which displays minor fitness defects.^1 In this study, we have created a new yeast strain which contains a single circular chromosome and apparently has not been found in nature. | Yangyang Shao Ning Lu Chen Cai Fan Zhou Shanshan Wang Zhihu Zhao Guoping Zhao Jin-Qiu Zhou Xiaoli Xue Zhongjun Qin | 2019 | Cell Research2019，29，1： | 5 |
| 3 | The size of cell-free mitochondrial DNA in blood is inversely correlated with tumor burden in cancer patients显示文摘Circulating cell-free DNAs(cfDNAs)are fragmented DNA molecules released into the blood by cells.Previous studies have suggested that mitochondria-originated cfDNA fragments(mt-cfDNAs)in cancer patients are more fragmented than those from healthy controls.However,it is still unknown where these short mtcfDNAs originate,and whether the length of mt-cfDNAs can be correlated with tumor burden and cancer progression.In this study,we first performed whole-genome sequencing analysis(WGS)of cfDNAs from a human tumor cell line-xenotransplantation mouse model and found that mt-cfDNAs released from transplanted tumor cells were shorter than the mouse counterpart.We next analyzed blood cfDNA samples from hepatocellular carcinoma and prostate cancer patients and found that mt-cfDNA lengths were inversely related to tumor size as well as the concentration of circulating tumor DNA.Our study suggested that monitoring the size of mt-cfDNAs in cancer patients would be a useful way to estimate tumor burden and cancer progression. | Qin An Youjin Hu Qingjiao Li Xufeng Chen Jiaoti Huang Matteo Pellegrini Xianghong Jasmine Zhou Matthew Rettig Guoping Fan | 2019 | Precision Clinical Medicine2019，2，3： | 4 |
| 4 | mTOR regulates NLRP3 inflammasome activation via reactive oxygen species in murine lupus显示文摘 | Xiaojing Li Xuefang Zhang Yuchen Pan Guoping Shi Jing Ren Hongye Fan Huan Dou Yayi Hou | 2018 | Acta Biochimica et Biophysica Sinica2018，50，9： | 4 |
| 5 | Dendrimers as carriers for contrast agents in magnetic resonance imaging显示文摘Magnetic resonance imaging (MRI) is a non-invasive clinical imaging modality,which has become widely used in the diagnosis of human diseases around the world.Some MRI exams include the use of contrast agents.The goal of an ideal MRI contrast agent involves the tissue-or organ-targeting materials with high relaxivity and specificity,low toxicity and side effects,suitable long intravascular duration and excretion time and high contrast enhancement with low doses,in vivo,all coupled to low overall cost.Dendrimers are synthetic,highly branched,mono-disperse macromolecules of nanometer dimensions.Properties associated with these dendrimers such as uniform size,water solubility,modifiable surface functionality and available internal cavities make them candidates for ideal carriers of MRI contrast agents.The research progress of the dendritic contrast agents is discussed. | YAN GuoPing AI ChaoWu LI Liang ZONG RongFeng LIU Fan | 2010 | Chinese Science Bulletin2010，55，27： | 3 |
| 6 | Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR显示文摘The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1,but also provide the strategies to monitor other new types ofinfluenza virus with explosive potential. | YAN An DING GuoHui ZHOU ZhenFeng DONG Hui ZHANG YaKun ZHU Lei HE YunGang ZHANG GuoQing LI YiXue SUN Bing HUANG Zhong LAN Ke JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenGan ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN WeiRong XIONG Hui | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 7 | The mutation network for the hemagglutinin gene from the novel influenza A (H1N1) virus显示文摘A mutation network for the hemagglutinin gene(HA) of the novel type A(H1N1) influenza virus was constructed.Sequence homology analysis indicated that one HA sequence type from the viruses mainly isolated from Mexico was likely the original type in this epidemic.Based on the 658A and 1408T mutations in HA,the viruses evolving into this epidemic were divided into three categories,the Mexico,the transitional and the New York type.The three groups of viruses presented distinctive clustering features in their geographic distributions. | HE YunGang DING GuoHui BIAN Chao HUANG Zhong LAN Ke SUN Bing WANG XueCai LI YiXue WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang JIN WeiRong XIONG Hui DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenAn ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN Li | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 8 | Sharpness of some properties of weighted modulation spaces显示文摘We obtain some optimal properties on weighted modulation spaces. We find the necessary and sufficient conditions for product inequalities, convolution inequalities and embedding on weighted modulation spaces. Especially, we establish the analogue of the sharp Sobolev embedding theorem on weighted modulation spaces. | GUO WeiChao FAN DaShan WU HuoXiong ZHAO GuoPing | 2016 | Science China Mathematics2016，59，1： | 3 |
| 9 | Experimentai Research on Saturated-Gain for Soft X-Ray Laser from Neon-Like Germanium Plasma显示文摘AmpliHcation of spontaneous emissions at 19.6,23.2 and 23.6 nm have been observed by a “ultiple-Target Series Coupling”design in Ge plasma,.The combined length for four targets is up to 56mm.The gain length product(GL)of small signal is up to 18 for both lines at 23.2 and 23.6 nmt and the effective GL is 16.4 and 15.7 for these two lines respectively.This two lines are obviously tending to saturation.The divergence of x-ray laser beam is about 4 mrad. | WANG Shiji GU Yuan ZHOU GuanUn NI Yuanlong YU Songyu FU Sizu MAO Chusheng TAO Zucong CHEN Wannian LIN Zunqi FAN Dianyuan ZHANG Guoping SHENG Jiatian YANG Minglun ZHANG Tanxin SHAO Yunfeng PENG Huimin HE Xiantu YU Min | 1991 | Chinese Physics Letters1991，8，12： | 3 |
| 10 | Genotypic variation in spatiotemporal distribution of canopy light interception in relation to yield formation in cotton显示文摘Background: Within-canopy interception of photosynthetically active radiation(PAR) impacts yield and other agronomic traits in cotton(Gossypium hirsutum L.). Field experiments were conducted to investigate the influence of 6 cotton varieties(they belong to 3 different plant types) on yield, yield distribution, light interception(LI), LI distribution and the relationship between yield formation and LI in Anyang, Henan, in 2014 and 2015.Result: The results showed that cotton cultivars with long branches(loose-type) intercepted more LI than did cultivars with short branches(compact-type), due to increased LI in the middle and upper canopy. Although loose-type varieties had greater LI, they did not yield significantly higher than compact-type varieties, due to decreased harvest index. Therefore, improving the harvest index by adjusting the source-to-sink relationship may further increase cotton yield for loose-type cotton. In addition, there was a positive relationship between reproductive organ biomass accumulation and canopy-accumulated LI, indicating that enhancing LI is important for yield improvement for each cultivar. Furthermore, yield distribution within the canopy was significantly linearly related to vertical LI distribution.Conclusion: Therefore, optimizing canopy structure of different plant type and subsequently optimizing LI distribution within the cotton canopy can effectively enhance the yield. | XING Fangfang HAN Yingchun FENG Lu ZHI Xiaoyu WANG Guoping YANG Beifang FAN Zhengyi LEI Yaping DU Wenli WANG Zhanbiao XIONG Shiwu LI Xiaofei Ll Yabing | 2018 | Journal of Cotton Research2018，1，3： | 3 |
| 11 | Structure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus显示文摘In recent months,a novel influenza virus H1N1 broke out around the world.With bioinformatics technology,the 3D structure of HA protein was obtained,and the epitope residues were predicted with the method developed in our group for this novel flu virus.58 amino acids were identified as potential epitope residues,the majority of which clustered at the surface of the globular head of HA protein.Although it is located at the similar position,the epitope of HA protein for the novel H1N1 flu virus has obvious differences in the electrostatic potential compared to that of HA proteins from previous flu viruses. | WU Di XU TianLei SUN Jing DAI JianXin DING GuoHui HE YunGang ZHOU ZhengFeng XIONG Hui DONG Hui JIN WeiRong BIAN Chao JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang WANG Hao CHE XiaoYan HUANG Zhong LAN Ke SUN Bing WU Fan YUAN ZhenAn ZHANG Xi ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi GUO YaJun ZHAO GuoPing LI YiXue CAO ZhiWei | 2009 | Chinese Science Bulletin2009，54，13： | 2 |
| 12 | Efficient circular gene knockout system for Burkholderiales strain DSM 7029 and Mycobacterium smegmatis mc^2 155显示文摘Multiple gene knockouts are often employed in studies of microbial physiology and genetics. However, the selective markers that confer antibiotic resistance are generally limited, so it is necessary to remove these resistance genes before the next round of using, which is time consuming and labor intensive. Here, we created a universal circular gene knockout system for both the gram-negative bacterial Burkholderiales strain DSM 7029 and the gram-positive bacterial Mycobacterium smegmatis mc^2 155, by combining the homologous recombination with multiple serine integrase-meditated site-specific recombination systems. In this system, a resistance gene and an integrase gene were constructed within the two attachment sites corresponding to a second, different integrase to form a cassette for gene disruption, which could be easily removed by the second integrase during the subsequent round of gene knockout. The sacB gene was also employed for negative selection. As the integrase-mediated deletion of the resistance/integrase gene cassette was highly efficient and concurrent with the following knockout round, the cyclic use of three cassettes could achieve multiple gene knockout in a sequential manner. Following the modularity concept in synthetic biology, common components of the knockout plasmids were retained as BioBricks, accelerating the knockout plasmids construction process. The circular gene knockout system can also be used for multiple gene insertions and applied to other microorganisms. | Xiaolai Lei Qiuxia Fan Tian Huang Haiyun Liu Guoping Zhao Xiaoming Ding | 2019 | Acta Biochimica et Biophysica Sinica2019，51，7： | 2 |
| 13 | Integrated transcriptome analysis of human iPS cells derived from a fragile X syndrome patient during neuronal differentiation显示文摘Fragile X syndrome(FXS) patients carry the expansion of over 200 CGG repeats at the promoter of fragile X mental retardation 1(FMR1), leading to decreased or absent expression of its encoded fragile X mental retardation protein(FMRP). However, the global transcriptional alteration by FMRP deficiency has not been well characterized at single nucleotide resolution, i.e., RNA-seq. Here,we performed in-vitro neuronal differentiation of human induced pluripotent stem(iPS) cells that were derived from fibroblasts of a FXS patient(FXS-iPSC). We then performed RNA-seq and examined the transcriptional misregulation at each intermediate stage during in-vitro differentiation of FXS-iPSC into neurons. After thoroughly analyzing the transcriptomic data and integrating them with those from other platforms, we found up-regulation of many genes encoding TFs for neuronal differentiation(WNT1, BMP4,POU3F4, TFAP2 C, and PAX3), down-regulation of potassium channels(KCNA1, KCNC3, KCNG2, KCNIP4, KCNJ3, KCNK9,and KCNT1) and altered temporal regulation of SHANK1 and NNAT in FXS-iPSC derived neurons, indicating impaired neuronal differentiation and function in FXS patients. In conclusion, we demonstrated that the FMRP deficiency in FXS patients has significant impact on the gene expression patterns during development, which will help to discover potential targeting candidates for the cure of FXS symptoms. | Ping Lu Xiaolong Chen Yun Feng Qiao Zeng Cizhong Jiang Xianmin Zhu Guoping Fan Zhigang Xue | 2016 | Science China(Life Sciences)2016，59，11： | 2 |
| 14 | Symptom clustering in chronic gastritis based on spectral clustering显示文摘OBJECTIVE: Apply spectral clustering to analyze the patterns of symptoms in patients with chronic gastritis(CG).METHODS: Based on 919 CG subjects, we applied mutual information feature selection to choose the positively correlated symptoms with each pattern.Then, we used the Shi and Malik spectral clustering algorithm to select the top 20 correlated symptoms.RESULTS: We ascertained the results of six patterns.There were three categories for the pattern of accumulation of damp heat in the spleen-stomach(0.00332). There were six categories for the pattern of dampness obstructing the spleen-stomach(0.02466). There were two categories for the pattern of spleen-stomach Qi deficiency(0.013 89).There were three categories for the pattern of spleen-stomach deficiency cold(0.009 15). There were five categories for the pattern of liver-Qistagnation(0.01910).There were four categories for the pattern of stagnant heat in the liver-stomach(0.00585).CONCLUSION: Most of the spectral clustering results of the symptoms of CG patterns were in accordance with clinical experience and Traditional Chinese Medicine theory. Most categories suggested the nature and/or location of the disease. | Wenhua Zhu Zhaoxiang Fan Guoping Liu Jianjun Yan Tao Zhong Wu Zheng Ruiqing Wang Chunying Wang | 2014 | Journal of Traditional Chinese Medicine2014，34，4： | 2 |
| 15 | Application of a hybrid MPI/OpenMP approach for parallel groundwater model calibration using multi-core computers显示文摘 | Tang Guoping D'Azevedo EF Zhang Fan | 2010 | Computers&Geosciences2010，36，11： | 1 |
| 16 | Synchron- ization between two complex dynamical networks using scalar under pinging control 显示文摘 | FAN Chunxia JIANG Guoping JIANG Fenghui | 2010 | IEEE Transactions on Circuits and Systems2010，57，11： | 1 |
| 17 | Pancreatic β Cell Identity Is Maintained by DNA Methylation-Mediated Repression of Arx显示文摘 | Sangeeta Dhawan Senta Georgia Shuen-ing Tschen Guoping Fan Anil Bhushan | 2011 | Developmental Cell2011，，4： | 1 |
| 18 | Synthesis of photo-reactive poly(vinyl alcohol)and construction of scaffold-free cartilage like pellets in vitro显示文摘Photo-reactive poly(vinyl alcohol)(PRPVA)was synthesized by introduction of phenyl azido groups into poly(vinyl alcohol)(PVA)and applied for surface modification.PRPVA was grafted onto cell culture plate surface homogeneously or in a micropattern.Human mesenchymal stem cells(hMSCs)cultured on cell culture plate surface and PVA-modified surface showed different behaviors.Cells adhered and spread well on cell culture plate surface,while they did not adhere on PVA-grafted surface at all.When hMSCs were cultured on PVA-micropatterned surface,they formed a cell micropattern.Cells formed pellets after cultured on PVA homogeneously modified surface in chondrogenic induction medium for 2 weeks.The pellets were positively stained by hematoxylin/eosin,safranin-O/fast green and toluidin blue,and they were also stained brown by Type II collagen and proteoglycan immunohistological staining.Real-time PCR analysis was conducted to investigate the expression of colI,colII,colX,aggrecan and sox9 mRNA.Results of gene expression were in agreement with those of histological and immunohistological observations.These results indicated that hMSCs cultured on PVA-modified surface performed chondrogenic differentiation,and it was possible to construct scaffold-free cartilage like pellets with PVA-modified surface in vitro. | Bao Li Yongli Gao Likun Guo Yujiang Fan Naoki Kawazoe Hongsong Fan Xingdong Zhang Guoping Chen | 2018 | Regenerative Biomaterials2018，5，3： | 1 |
| 19 | L-Phe end- capped poly (L-lactide) as macroinitiator for the synthesis of poly ( L-lactide)-b-poly (L-lysine) block copolymer 显示文摘 | Fan Yujiang Chen Guoping Tanaka J | 2005 | Bio- macromolecules2005，6，6： | 1 |
| 20 | L-Phe end-capped poly-(L-lactide) as macroinitiator for the synthesis of poly(L lactide)-b-poly(L-lysine) block copolymer显示文摘 | Fan Yujiang Chen Guoping Junzo Tanaka | 2005 | Biomacromolecules2005，6，6： | 1 |
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