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| 1 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 2 | 施万细胞在阴茎和外周神经再生中的作用显示文摘施万细胞(SCs)是外周神经系统中主要的胶质细胞。施万细胞最终发育为形成髓鞘的细胞和不形成髓鞘的细胞,前者包绕直径较大的神经,后者则包绕直径较小的神经。他们在神经损伤后的再生中发挥重要作用,包括海绵体神经损伤导致的勃起功能障碍(ED)。尽管前列腺癌根治术手术技术有所提高,但是仍有相当一部分患者术后出现ED。这是由于术中牵拉损伤或局部炎症反应改变了自主神经的微环境,最终导致终末器官-阴茎的病理生理改变。该综述目的是总结现有的如下方面相关文献:(1)外周神经特别是阴茎神经中施万细胞的起源和发育;(2)外周神经特别是阴茎神经损伤后的Wallerian变性和施万细胞的可塑性;(3)施万细胞如何通过分泌神经生长因子促进神经再生;(4)以施万细胞作为治疗靶点促进神经再生的技术。我们搜索了Pubmed,发现了为数众多的与上述主题相关的动物实验和人体研究,这提示施万细胞有望成为神经源性ED治疗的新靶点。 | Lin Wang Melissa T Sanford Zhongcheng Xin Guiting Lin Tom F Lue | 2015 | Asian Journal of Andrology2015，17，5： | 7 |
| 3 | Possibility of the Application of the Ultra-long Electromagnetic Wave Remote Sensor to Marine Geological Exploration显示文摘The ultra-long electromagnetic wave remote sensing technique developed by Peking University is one of new future techniques, which can detect the submarine geological information from the depth of 20 to 10000 m below the surface by receiving natural ultra-long electromagnetic waves (n Hz to n 100 Hz). The new remote sensor is composed of three parts: a main instrument with a portable computer, an antenna with an amplifier and an external power.The new remote sensing technique is characterized by good stability and reproducibility at the same spot but at different times and high sensitivity and high signal-to-noise ratio, and can reveal geological and lithologic boundaries as well as strata and related mineral sources. Two years of marine geological experiments on this technique have indicated that it can solve many problems in marine geological exploration, e.g. the burial depths of sea-floor mud, Quaternary sediments and submarine structures. This technique can be applied to detecting the sea bed | HOU Guiting QIAN Xianglin WANG Shuyuan SUN Jing | 2000 | Acta Geologica Sinica(English Edition)2000，74，2： | 6 |
| 4 | Tunica albuginea allograft: a new model of LaPeyronie's disease with penile curvature and subtunical ossification显示文摘LaPeyronie 的疾病(PD ) 的 pathophysiology 被认为是 multifactorial,包含愈合的基因倾向,损伤,发炎和改变的创伤。然而,这些因素还没用动物模型被验证了。在这研究,我们介绍了 tunica albuginea allograft 获得的一个新模型。40 的一个总数, 16-week-old 男性老鼠被使用。这些, 8 只老鼠用作控制并且经历了 10 ×有随后的 autografting 的 2-mm-wide tunical 切除,而留下 32 与另一只老鼠的 tunica 与缺点的 grafting 经历了一样的切除。词法、功能测试在 grafting 以后在 1, 3, 7 和 12 个星期被执行。Intracavernous 压力,阴茎弯曲的度和有弹性的纤维长度为在 allograft 和控制组之间的比较被评估。纸巾为组织学的检查被获得。阴茎弯曲作为与控制老鼠相比在 allografted 老鼠是显著地更大的。可勃起的功能在所有老鼠被维持,除了在在 12 个星期估计的那些。弹力素纤维长度作为与控制相比在 allografted tunica 被减少。SMAD2 表示在 allograft 的内部部分被检测,并且 collagen-II- 和 osteocalcin 积极的房间也被注意。在老鼠的 Tunica albuginea (TA ) allograft 是 PD 的一个优秀模型。在 12 个星期和在 TA 的内部层的骨化的存在以后的弯曲的坚持类似于与 PD 在人观察的那些。用这个动物模型的确认研究将为有效治疗学的干预帮助 PD pathophysiology 的理解。 | Ludovic Ferretti Thomas M Fandel Xuefeng Qiu Haiyang Zhang Hazem Orabi Alex K Wu Lia Banie Guifang Wang Guiting Lin Ching-Shwun Lin Tom FLue | 2014 | Asian Journal of Andrology2014，16，4： | 3 |
| 5 | Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing显示文摘Base editing, as an expanded clustered regularly interspaced short palindromic repeats(CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus(SaCas9), is important in genome editing because it can edit sites with HHHAAT protospacer adjacent motif(PAM) that the canonical Streptococcus pyogenes Cas9(SpCas9) or its variants(e.g. xCas9, Cas9-NG) cannot. However, several technical parameters of SaKKH involved base editors have not been well defined and this uncertainty limits their application. We developed an effective multiplex cytosine base editor(SaKKHn-pBE) and showed that it recognized NNARRT, NNCRRT, NNGRGT, and NNTRGT PAMs. Based on 27 targets tested, we defined technical parameters of SaKKHn-pBE including the editing window, the preferred sequence context, and the mutation type. The editing efficiency was further improved by modification of the SaKKH sgRNA. These advances can be applied in future research and molecular breeding in rice and other plants. | Chengwei Zhang Feipeng Wang Si Zhao Guiting Kang Jinling Song Lu Li Jinxiao Yang | 2020 | The Crop Journal2020，8，3： | 1 |
| 6 | An automatic bridge detection technique for high resolution SAR images显示文摘 | Guiting Wang Shah Huang Li Chengjiao | | 0，，： | 1 |
| 7 | Power generation expansion planning approach considering carbon emission constraints显示文摘Decarbonization of the power sector in China is an essential aspect of the energy transition process to achieve carbon neutrality.The power sector accounts for approximately 40%of China’s total CO_(2) emissions.Accordingly,collaborative optimization in power generation expansion planning(GEP)simultaneously considering economic,environmental,and technological concerns as carbon emissions is necessary.This paper proposes a collaborative mixedinteger linear programming optimization approach for GEP.This minimizes the power system’s operating cost to resolve emission concerns considering energy development strategies,flexible generation,and resource limitations constraints.This research further analyzes the advantages and disadvantages of current GEP techniques.Results show that the main determinants of new investment decisions are carbon emissions,reserve margins,resource availability,fuel consumption,and fuel price.The proposed optimization method is simulated and validated based on China’s power system data.Finally,this study provides policy recommendations on the flexible management of traditional power sources,the market-oriented mechanism of new energy sources,and the integration of new technology to support the attainment of carbon-neutral targets in the current energy transition process. | Hasan Mehedi Xiaobin Wang Shilong Ye Guiting Xue Islam Md Shariful Fang Shi | 2023 | Global Energy Interconnection2023，6，2： | 1 |
| 8 | Treatment of stress urinary incontinence with adipose tissue-derived stem cells显示文摘 | Guiting Lin Guifang Wang Lia Banie Hongxiu Ning Alan W. Shindel Thomas M. Fandel Tom F. Lue Ching-Shwun Lin | 2010 | Cytotherapy2010，，1： | 1 |
| 9 | Treatment of stress urinary incontinence with adipose tissue-derived stem cells显示文摘 | Guiting Lin Guifang Wang Lia Banie Hongxiu Ning Alan W. Shindel Thomas M. Fandel Tom F. Lue Ching-Shwun Lin | 2010 | Cytotherapy2010，，1： | 1 |
| 10 | Late Paleoproterozoic extension and a paleostress field reconstruction of the North China Craton显示文摘 | Guiting Hou Chuancheng Wang Jianghai Li Xianglin Qian | 2006 | Tectonophysics2006，，1： | 1 |
| 11 | Treatment of stress urinary incontinence with adipose tissue-derived stem cells显示文摘 | Guiting Lin Guifang Wang Lia Banie Hongxiu Ning Alan W. Shindel Thomas M. Fandel Tom F. Lue Ching-Shwun Lin | 2010 | Cytotherapy2010，，1： | 1 |
| 12 | Molecular Yin and Yang of erectile function and dysfunction显示文摘关于可勃起的功能,殷是软弱和杨勃起。在过去的十年,研究主要集中于可勃起的功能的杨方面。在最近的年里,然而,殷方面正在吸引逐渐地更大的注意。这由于阴茎软弱比阴茎勃起同样地重要并且被在可勃起的机能障碍(编辑) 的某些类型起关键作用的机制活跃地维持的实现;例如,在糖尿病的病人。另外,有证据,表明小径的殷和杨从软弱在转变期间与对方一起交往到勃起,并且反过来也如此。因此,我们从不仅杨而且殷方面看可勃起的功能,是重要的。这篇文章的目的是在调整阴茎的殷和杨的分子的机制的理解考察最近的进展。强调被给表明调整殷的小径的 Rho kinase,并且到表明调整杨的小径的周期的核苷酸。讨论以如此的一个方法被组织以便跟随发信号的串联,也就是说以细胞外的发信号分子开始(例如, norepinephrin 和氮的氧化物)并且他们的受体,收敛到细胞内部的受动器上(例如, Rho kinase 和蛋白质 kinase G ),分叉进第二等的受动器,并且与可收缩的分子和 phosphodiesterases 完成。在表明小径的殷和杨之间的相互作用也被讨论。 | Ching-Shwun Lin Zhong-Cheng Xin Zhong Wang Guiting Lin Tom E Lue | 2008 | Asian Journal of Andrology2008，10，3： | 1 |
| 13 | Adipose Derived Stem Cells Ameliorate Hyperlipidemia Associated Detrusor Overactivity in a Rat Model显示文摘 | Yun-Ching Huang Alan W. Shindel Hongxiu Ning Guiting Lin Ahmed M. Harraz Guifang Wang Maurice Garcia Tom F. Lue Ching-Shwun Lin | 2010 | The Journal of Urology2010，，3： | 1 |
| 14 | Late Paleoproterozoic extension and a paleo - stress field reconstruc- tion of the North China Craton 显示文摘 | Guiting Hou Chuancheng Wang Jianghai Li Xianglin Qian | 2006 | Tectonophysics2006，422，14： | 1 |
| 15 | Electrochemical construction of porous gold nanostructures on DVD substrate and its application as nonenzymatic hydrogen peroxide sensor显示文摘A simple acid-etching method was used to leach out silver from a bimetallic gold-silver based Digital Video Disc(DVD), which was further treated via a cyclic voltammetric scanning in 1 mmol/L HAu Cl4 solution to create a porous gold nanostructure. The as-fabricated electrode was characterized by field-emission scanning electron microscopy, energy-dispersed X-ray spectroscopy and X-ray diffraction. The high electro-catalytic activity of the resulting electrode toward the reduction of hydrogen peroxide(H2O2) presented excellent linear relationship in the range of 8.0×10?5 to 1.26×10?2 mol/L with the detection limit of 2.0×10-5 mol/L(S/N=3). The as-developed non-enzyme-sensor showed good reproducibility, stability, and selectivity. | Huifen Chen Xiaoping Wang Guiting Liu Aijing Lin Ying Wen Haifeng Yang | 2015 | Science China Chemistry2015，58，10： | 0 |
| 16 | Influencing factors of using behaviour for computational advertising under the theoretical model of UTAUT显示文摘Based on the UTAUT theoretical model, this paper discusses the applicability of the model incomputational advertising situations. Computational advertising relies on artificial intelligencetechnologies such as big data, cloud computing, new algorithms, and blockchain. Results showthat: performance expectation, effort expectation, and social impact have a positive influence onthe acceptance intention of computational advertising;stimulus has no significant influence onthe using behaviour of computational advertising;the acceptance intention of computationaladvertising has a positive influence on the using behaviour of computational advertising. Fourpoints should be paid attention to in the future computational advertising: paying attention tothe needs of users and realising accurate advertising;improving efforts and expectations andoptimising user experience;enhancing user interaction and user stickiness;protecting user privacyand improving user satisfaction. The core of computational advertising research in the fieldof humanities and social sciences is not dislocated from the traditional user-centred problemconsciousness. | Guiting Wang Jiaying Xu Shengliang Lin | 2023 | Journal of Control and Decision2023，10，1： | 0 |
| 17 | Alterative application of five anticonvulsants according to the half life for the treatment of status epilepticus in children with severe viral encephalitis显示文摘BACKGROUND: Traditional subhibernation therapy may easily cause complications, such as respiratory depression and hyportension because of application of chlorpromazine hydrochloride and promethazine in a large dosage. OBJECTIVE: To observe therapeutic effect of modified subhibernation therapy (alterative application of five anticonvulsants according to the half life) on status epilepticus in children with severe viral encephalitis (VE). DESIGN: Contrast observation. SETTING: Department of Pediatrics, the First Hospital of Jilin University. PARTICIPANTS: The participants in present study were 96 patients with severe viral encephalitis including 52 boys and 44 girls who received treatment in the Department of Pediatrics, the First Hospital of Jilin University from February 2000 to March 2006. All children met the diagnostic criteria of Zhufutong Practice Pediatrics (the seventh edition). Two weeks ago, they ever got upper respiratory infection or enteronitis and so on before the onset, spirit abnormal, behavior disorder, limbs act disorder, vomit, headache, convulsion, nervous system masculine signs such as limbs act disord, autonomic nerve damage manifestation, brain nerve palsy, dysreflexia, meningeal irritation sign, cerebrospinal fluid and electroencephalography (EEG) abnormity. All parents provided the confirmed consent. The patients were randomly divided into control group (n =40) and experimental group (n =56). METHODS: Patients in the control group received anticonvulsion, ice compress and routine treatment. The convulsion was treated with five drugs: 0.5 mg/kg wintermin and phenergan, respectively, 100 g/L chlorpromazine hydrochloride (0.5 mL/kg), 5 mg/kg luminal, 0.3 mg/kg ansiolin. When convulsion attacked, those five drugs were given alternatively; however, those were not given if the convulsion did not attack. Children in the experimental group were treated with improved subhibernation therapy based on routine treatment. The dosages of anticonvulsants were as the same as those in the control group. Based on the half life, every drug was alternated every 4-6 hours. In addition, anticonvulsants administrated for 2 successive days whether tic attacked or not. Then the hypnotic was removed gradually. MAIN OUTCOME MEASURES: Therapeutic efficacy, time of disappeared clinical symptoms and physical sign, and security of administration. RESULTS: All the 96 patients were involved in the final analysis. ① Total effective rate and reliability: Total effective rate was higher in the treatment groups than the control group (χ2=5.871 7, P < 0.05). All patients did not have respiratory depression and side effects. ② Time of disappeared clinical symptoms and physical sign: Recovery time of convulsion, fever, headache and vomit was shorter in the treatment group than that in the control group, and there was significant difference (t =17.612 1-34.330 7, P < 0.05); in addition, symptoms of status epilepticus were relieved obviously. Meanwhile, recovery time of paralysis, coma and anepia was shorter in the treatment group than that in the control group, and there was significant difference (t =10.660 8-24.700 8, P < 0.05). CONCLUSION: Therapeutic effect of improved subhibernation therapy on status epilepticus induced by severe viral encephalitis is positively and safer. | Yanzhi Huang Libin Yang Shuqing Wang Guiting Wang | 2007 | Neural Regeneration Research2007，2，9： | 0 |
| 18 | Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.显示文摘Sesame Fusarium wilt(SFW),caused by Fusarium oxysporum f.sp.sesami(Fos),is one of the most devastating diseases affecting sesame cultivation.Deciphering the genetic control of SFW resistance is pivotal for effective disease management in sesame.An inheritance study on a cross between the highly resistant variety Yuzhi 11 and the highly susceptible accession Sp1 using a Fos pathogenicity group 1 isolate indicated that resistance was conferred by a single dominant allele.The target locus was located in a 1.24 Mb interval on chromosome 3 using a combination of cross-population association mapping and bulked segregant analysis.Fine genetic mapping further narrowed the interval between 21,350 and 21,401 kb.The locus Sindi_0812400 was identified as the SFW resistance gene and officially designated SiRLK1.This gene encodes a specific malectin/receptor-like protein kinase with three putative tandem kinase domains and is considered a kinase fusion protein.Sequence analysis revealed that a high proportion(49.44%)of variants within the locus was located within the kinase domainⅢ,and several of which were evidently associated with the diversity in SFW response,indicating the critical role of kinase domainⅢin expression of disease resistance.These findings provide valuable information for further functional analysis of SFW resistance genes and marker-assisted resistance breeding in sesame. | Yinghui Duan Wenwen Qu Shuxian Chang Ming Ju Cuiying Wang Cong Mu Hengchun Cao Guiting Li Qiuzhen Tian Qin Ma Zhanyou Zhang Haiyang Zhang Hongmei Miao | 2024 | The Crop Journal2024，12，1： | 0 |
| 19 | L-Arginine based polyester amide/hyaluronic acid hybrid hydrogel with dual anti-inflammation and antioxidant functions for accelerated wound healing显示文摘Nowadays, there are still many challenges to skin regeneration. As a new type of skin substitute, hydrogel has emerging gradually with its excellent properties. However, it is still a challenge to combine with biological active agents to facilitate skin regeneration. Under the circumstance, we synthesized argininebased poly(ester amide)(Arg-PEA) and hyaluronic acid(HA-MA), and combined them into new hybrid hydrogels via photo-crosslinking. We found that the internal structure and physicochemical properties of hybrid hydrogels were greatly improved with the increase of content of Arg-PEA. Therefore, we designed hybrid hydrogels with 5 wt% and 10 wt% of Arg-PEA content, respectively. Besides, we selected the corresponding anti-inflammatory(CRP, TNF-α) indicators to detect the anti-inflammatory properties of the hybrid hydrogels at the protein level, and the corresponding antioxidant indicators(SOD, GSH/GSSG, MDA)were selected to investigate the antioxidant properties of hybrid hydrogels at the cellular level in vitro.In addition, we also selected relevant genes to test the effect of hybrid hydrogels on fibrosis and vascularization in the process of skin wound healing in vitro and verified them in vivo with a mouse dorsum wound model. The results confirmed that Arg-PEA/HA-MA(AH) hybrid hydrogel was a prospective scaffold material for skin regeneration. | Tong Liu Guiting Liu Jianhua Zhang Zhangfan Ding Yike Li Krishna Sigdel Xiaoyi Wang Huixu Xie | 2022 | Chinese Chemical Letters2022，33，4： | 0 |
| 20 | UroPrecision(UP):Turn UPthevolume of precision medicine in urology显示文摘More than 100 years ago,urology was recognized as an independent specialty.It is a dynamic and rapidly evolving field that plays a critical role in the diagnosis,treatment,and management of a wide range of urologic disorders.Over the past few decades,a splendid range of diagnostic options and therapeutic strategies in urooncology,endourology,female urology,and andrology has emerged.In particular,the explosion in precision medicine in the field of urology has revolutionized the way urologic malignancies are treated,and has led research and discovery on a new journey. | Nianzeng Xing Gang Song Guiting Lin Run Wang | 2023 | UroPrecision2023，1，1： | 0 |
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