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| 1 | Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System显示文摘Dear Editor,Base editors(BEs)based on the CRISPR/Cas9 system,including cytosine base editors and adenine base editors,which can efficiently perform four transition mutations(C·G-to-T·A and A·T-to-G·C),have been well studied and widely used to produce base mutations in a variety of organisms,including in plants such as rice. | Wen Xu Chengwei Zhang Yongxing Yang Si Zhao Guiting Kang Xiaoqing He Jinling Song Jinxiao Yang | 2020 | Molecular Plant2020，13，5： | 20 |
| 2 | Recent advances in andrology-related stem cell research显示文摘因为他们自我更新并且到的能力,干细胞为再生药保持大诺言区分进各种各样的房间类型。尽管胚胎的干细胞(二进制同步通信) 比成年干细胞有更大的区别潜力,前者在因为道德的担心和政府限制,到达临床的应用程序正在落后。骨头髓干细胞(BMSC ) 是学习最好的成年干细胞(ASC ) 并且有潜力治疗许多疾病,包括可勃起的机能障碍(编辑) 和男不孕。更最近发现的脂肪质的导出织物的干细胞(ADSC ) 与在区别和治疗学的潜力的骨头髓干细胞几乎相同,但是更容易、更安全获得,能在更大的数量被收获,并且有减少肥胖的联系利益。因此, ADSC 看起来是为未来的一种更好的选择临床的应用。我们以前证明了转换字符能在老鼠恢复神经原的编辑的可勃起的功能,并且我们现在有 ADSC 能那么也做的证据。我们也正在调查 ADSC 是否能区分进 Leydig, Sertoli 和男细菌房间。最终的目标是使用 ADSC 对待男不孕和睾丸激素缺乏。 | Ching-Shwun Lin Zhong-Cheng Xin Chun-Hua Deng Hongxiu Ning Guiting Lin Tom F. Lue | 2008 | Asian Journal of Andrology2008，10，2： | 18 |
| 3 | Insights into the Tectonic Fractures in the Yanchang Formation Interbedded Sandstone-Mudstone of the Ordos Basin Based on Core Data and Geomechanical Models显示文摘The distribution and intensity of tectonic fractures within geologic units are important to hydrocarbon exploration and development. Taken the Upper Triassic Yanchang Formation interbedded sandstone-mudstone in the Ordos Basin as an example, this study used the finite element method(FEM) based on geomechanical models to study the development of tectonic fractures. The results show that the sandstones tend to generate tectonic fractures more easily than mudstones with the same layer thickness, and the highest degree of tectonic fractures will be developed when the sandstone-mudstone thickness ratio is about 5.0. A possible explanation is proposed for the tectonic fracture development based on two important factors of rock brittleness and mechanical layer thickness. Generally, larger rock brittleness and thinner layer thickness will generate more tectonic fractures. In interbedded sandstone-mudstone formations, the rock brittleness increases with the increasing mechanical layer thickness, hence, these two factors will achieve a balance for the development of tectonic fractures when the sandstone-mudstone thickness ratio reaches a specific value, and the development degree of tectonic fractures is the highest at this value. | JU Wei SUN Weifeng HOU Guiting | 2015 | Acta Geologica Sinica(English Edition)2015，89，6： | 16 |
| 4 | Mesozoic-Cenozoic extension of the Bohai Sea: contribution to the destruction of North China Craton显示文摘Bohai 海是位于东方诺思中国 Craton (NCC ) 的盆山脉系统的一个迟了的中生代新生代特征。岩石圈变瘦的迟了的中生代表明 NCC 的早破坏。破坏的发作由于 craton 的厚岩石圈的分层,在 Tanlu 差错地区以内向西在在军队飞机安排由趋势 NW grabens,并且由趋势 NNE grabens 代表了到 Tanlu 差错。迟了的中生代趋势 NW grabens 被与新生代趋势 NE 拉开盆有关的结构在东方 NCC 与很厚的中生代新生代沉积套印。生长差错和延期因素的 C 频率图([贝它]) 四,越过盆的节建议延期从边缘移居了到 Bohai 海的中心,并且为 Bohai 海的中生代和新生代延期因素比边缘的高。这些证据建议最大的延期发生在 Bohai 海的中心,它与在海的中心被发现的最薄的外壳一致。延期比率和 Bohai 海的构造进化建议它是为 NCC 的破坏的关键区域,由地形学证实了。然而, NCC 与与东北亚洲的构造政体有关的迟了的阶段经历了破坏的二个阶段[出版摘要] | Guiting HOU K. R. HARI | 2014 | Frontiers of Earth Science2014，8，2： | 14 |
| 5 | The late Paleoproterozoic extension event:aulacogens and dyke Swarms in the North China craton显示文摘在北方中国 Craton (NCC ) 的延期结构和构造牵连基于 mafic 塘群和地球年代学在这篇论文被讨论,与 aulacogens 的地球年代学结合。迟了的 Paleoproterozoic 时间是在 NCC 的前寒武纪的进化的重要拐弯处点。延期系统(例如 aulacogens 和塘群) 在 NCC 是普遍的,它与类似于现代的板的僵硬特征标记 NCC 的碳化。当模特儿的 paleostress 地建议塘聚集, aulacogens 是标记超级大陆,不是 synorogenic 的开始的 arogenic 扩展和 postorogenic 扩展。在 NCC 的 mafic 塘群主要从 1.83 ~ 1.77 Ga 。NCC 的延期是很有限的易碎的扩展,平均延期比率仅仅是 0.35% 由 mafic 塘群延期计算给,大多数在 NCC 的扩展被 aulacogens 因此贡献。mafic 塘群在起源与 aulacogens 被联系。 | HOU Guiting LI Jianghai LIU Yulin QIAN Xianglin | 2006 | Progress in Natural Science:Materials International2006，16，2： | 13 |
| 6 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 7 | Fractal Simulation and Classification of Folds显示文摘The method of fractal simulation and classification of folds is firstly studied here to describe various types of complex fold patterns in quantitative analysis. Based on the characteristics of natural folds with a fractal pattern, the fold patterns are simulated to describe various types of folds quantitatively by means of fractal interpolation. The major factors affecting the fold pattern are elucidated in fractal simulation of folds, i.e. positions of interpolation points (x, y) and the disturbance coefficient d of folds (-1<d<1). The bigger the value d for a fold simulation is, the more complex or disturbed the folds are and the better developed the relative secondary folds are. If d>0, folds are up-convex. IF d<0, they are down-convex. |d|=0, |d|=0.25 and |d|=0.5 represent three conspicuous turning states. If |D|=0, the points will be joined by a straight line. If |d|=0.25, the points will be joined smoothly. If |d|>0.25, there will be complex secondary folds between the points. If |d| >0.5, | HOU Guiting | 1998 | Acta Geologica Sinica(English Edition)1998，72，2： | 7 |
| 8 | 施万细胞在阴茎和外周神经再生中的作用显示文摘施万细胞(SCs)是外周神经系统中主要的胶质细胞。施万细胞最终发育为形成髓鞘的细胞和不形成髓鞘的细胞,前者包绕直径较大的神经,后者则包绕直径较小的神经。他们在神经损伤后的再生中发挥重要作用,包括海绵体神经损伤导致的勃起功能障碍(ED)。尽管前列腺癌根治术手术技术有所提高,但是仍有相当一部分患者术后出现ED。这是由于术中牵拉损伤或局部炎症反应改变了自主神经的微环境,最终导致终末器官-阴茎的病理生理改变。该综述目的是总结现有的如下方面相关文献:(1)外周神经特别是阴茎神经中施万细胞的起源和发育;(2)外周神经特别是阴茎神经损伤后的Wallerian变性和施万细胞的可塑性;(3)施万细胞如何通过分泌神经生长因子促进神经再生;(4)以施万细胞作为治疗靶点促进神经再生的技术。我们搜索了Pubmed,发现了为数众多的与上述主题相关的动物实验和人体研究,这提示施万细胞有望成为神经源性ED治疗的新靶点。 | Lin Wang Melissa T Sanford Zhongcheng Xin Guiting Lin Tom F Lue | 2015 | Asian Journal of Andrology2015，17，5： | 7 |
| 9 | 阴茎硬结症的相关基因表达及TGF-β1的治疗效应显示文摘目的 筛选参入及调控阴茎硬结症的相关基因 ,探讨其发病机理及针对相关基因治疗的应答作用。方法 用基因芯片、细胞生物学和分子生物学技术对自行构建的三个阴茎硬结症细胞系进行研究 ,用TGF β1治疗后观察相关基因表达差异。 结果 MCP 1是参与阴茎硬结症病理过程的关键下游基因 ,在硬结及其周围组织构建的细胞系有高表达 (P <0 0 5 ) ,TGF β1治疗后明显上调其表达水平 (P <0 0 5 )。结论 MCP 1在阴茎硬结症的发病过程中起重要作用 ,是某些基因调控的下游基因 ,以此为靶基因的治疗将提高疗效、减少并发症。 | 王忠 刘景芳 周智恒 张元芳 王文健 张鹏 Yucel Selcuk Lin Guiting Lin Ching-shwun | 2004 | 中华外科杂志2004，42，3： | 7 |
| 10 | Possibility of the Application of the Ultra-long Electromagnetic Wave Remote Sensor to Marine Geological Exploration显示文摘The ultra-long electromagnetic wave remote sensing technique developed by Peking University is one of new future techniques, which can detect the submarine geological information from the depth of 20 to 10000 m below the surface by receiving natural ultra-long electromagnetic waves (n Hz to n 100 Hz). The new remote sensor is composed of three parts: a main instrument with a portable computer, an antenna with an amplifier and an external power.The new remote sensing technique is characterized by good stability and reproducibility at the same spot but at different times and high sensitivity and high signal-to-noise ratio, and can reveal geological and lithologic boundaries as well as strata and related mineral sources. Two years of marine geological experiments on this technique have indicated that it can solve many problems in marine geological exploration, e.g. the burial depths of sea-floor mud, Quaternary sediments and submarine structures. This technique can be applied to detecting the sea bed | HOU Guiting QIAN Xianglin WANG Shuyuan SUN Jing | 2000 | Acta Geologica Sinica(English Edition)2000，74，2： | 6 |
| 11 | Mechanism for three types of mafic dyke swarms显示文摘这研究建议三个模型解释 mafic 塘群的三种主要类型的机制。而小放射塘群在一座 Pluto 或暴烈的大厦附近由于压力构造形成,平行的塘群响应地区性的压力域形成,例如在北方中国 Craton 的 mafic 塘群,例如在西班牙的山峰的塘群,美国。散发塘群的第三种类型是象 Mackenzie 塘群那样的巨大的扇形的塘群。在 magmatism 以前形成了的破裂可以在支配塘群几何学起一个重要作用。在大多数盒子中,尽管岩浆注射能在塘尖端由繁殖增加破裂尺寸,先存在的破裂被构造压力并且不由岩浆注射导致。 | Guiting Hou | 2012 | Geoscience Frontiers2012，3，2： | 6 |
| 12 | 干细胞:治疗勃起功能障碍的新玩家显示文摘干细胞因其具有自我更新和定向分化的能力而得名,因此,它们赋予了再生医学巨大的潜力。从历史渊源看,干细胞曾被列为胚胎干细胞(ESCs)或成体干细胞(ASCs)。过去人们认为,只有胚胎干细胞具有分化成任何类型细胞的能力,而ASCs只有生成具有一个给定胚层细胞的能力。然而,最近许多研究表明ASCs具有分化成超出他们组织起源的细胞类型的能力。本综述总结了当代关于干细胞的可用性和分化的结果,尤其是关于这些细胞在动物模型和人体研究中诊断和治疗勃起功能障碍(ED)方面的潜力。我们在PubMed上进行文章搜索,包括干细胞的定义、定位和循环,以及干细胞在ED诊断和治疗方面的应用。目前,干细胞疗法作为未来潜在的治疗ED的方法这一概念已经被很多有利的证据支持。迄今为止,各种干细胞,包括骨髓间充质干细胞,脂肪源性干细胞和肌源性干细胞,已在ED治疗的神经,血管,内皮或平滑肌再生方面的动物模型中进行了研究。此外,几种成体干细胞的亚型分布于阴茎,循环内源性干细胞可以用来预测ED以及与ED有关的心血管疾病的治疗结果。 | Haiyang Zhang Maarten Albersen Xunbo Jin Guiting Lin | 2012 | Asian Journal of Andrology2012，14，1： | 6 |
| 13 | Wind vector retrieval using dual polarization imagery of ASAR显示文摘A new algorithm for retrieving wind vector using dual polarization imagery of advanced synthetic aperture radar (ASAR) is developed and tested. Based on the combination of co-polarization and cross-polarization, this new algorithm effectively eliminates the 180° ambiguity which occurs when using single imagery of the ASAR to retrieve wind vector. This algorithm also solves the problem that the retrieval results will break down on very small spatial scales. The results retrieved from dual polarization imagery of ASAR show that the wind speed and direction are in agreement with the data from Quikscat and buoy measurements. The root mean square errors of wind direction and speed between the retrieved results and data from Quikscat are 2.21° and 0.53 m/s, respectively. Comparisons between the new method and the traditional method show that the new methods fit the buoy data and Quikscat data a little better than the traditional method in this image. | SONG Guiting HOU Yijun QI Peng | 2006 | Progress in Natural Science:Materials International2006，16，11： | 5 |
| 14 | End Late Paleozoic tectonic stress field in the southern edge of Junggar Basin显示文摘这份报纸论述在由解释压力反应的 Junggar 盆的南部的边的晚古生代的构造压力地组织的结束(女同性恋,褶层,有岩石光滑面的差错并且结合关节) 。最大的主要压力轴的方向被解释是 NWSE (大约 325 牥污 ? 整浲吗? | Wei Ju Guiting Hou Le Li Fangfeng Xiao | 2012 | Geoscience Frontiers2012，3，5： | 5 |
| 15 | Emerging neuromodulatory molecules for the treatment of neurogenic erectile dysfunction caused by cavernous nerve injury显示文摘在细胞死亡的生长因素,神经发展,和预防的神经生物学的进展为多孔的神经( CN )为保护、再生的 neuromodulatory 策略的发展导致了提高的临床的兴趣,当为前列腺癌症和另外的骨盆的恶意的治疗经常导致神经元损坏和性功能的削弱的损失。氮的氧化物在平滑肌的阴茎海绵体原因松驰以内免除了这些神经的轴索终板,开始象贡献维持的肿大一样的阴茎勃起的 haemodynamic 变化;CN 功能的损失在骨盆的外科以后为可勃起的机能障碍(编辑) 的开发主要负责并且为潜在的 neuroprotective 或再生策略用作主要目标。从为精选 neuromodulatory 途径的现出症状之前的潜的研究的证据被考察,包括 neurotrophins,神经胶质房间导出线的神经营养的因素(GDNF ) ,骨头 morphogenic 蛋白质, immunophilin ligands, erythropoetin (EPO ) ,和干细胞。 | Anthony J. Bella Guiting Lin Ilias Cagiannos Tom F. Lue | 2008 | Asian Journal of Andrology2008，10，1： | 5 |
| 16 | Icariin on relaxation effect of corpus cavernosum smooth muscle显示文摘NO-cGMP pathway in penile corpus cavernosal smooth muscle plays an important role in penile erection. The level of cGMP is regulated by a balance between the rate of synthesis by guanylate cyclase and the rate of hydrolytic breakdown to guanosine 5’ monophosphate (GMP) by phos-phodiesterase 5(PDE5). Icariin is isolated from natural drug Epimedii herba, it is shown to have the relaxation effect on corpus cavernosal smooth muscle of rabbit (IC50: 4×10-4 mol/L), and the mechanism of the relaxation effect of Icariin on corpus cavernosum believed to have the inhibiting effect on PDE5 and activation of NO-cGMP pathway to enhancing penile erection. | XIN Zhongcheng Kim Euikyung TIAN Zhenji LIN Guiting QUO Yinglu | 2001 | Chinese Science Bulletin2001，46，14： | 4 |
| 17 | Injectable baicalin/F127 hydrogel with antioxidant activity for enhanced wound healing显示文摘Baicalin,extracted from traditional Chinese medicine Scutellaria baicalensis Georg,possesses multiple pharmacological activities and has great potential for chronic skin wound repair.However,the poor solubility and lack of suitable vehicles greatly limit its further application.Herein,we proposed a convenient and robust strategy,employing PBS solution as solvent,to enhance the solubility of baicalin.Furthermore,we constructed injectable baicalin/F127 hydrogels to study their application in skin wound treatment.The composition and temperature sensitivity of baicalin/Pluronic®F-127 hydrogels were confirmed by FTIR and rheological testing,respectively.In vitro release measurement indicated that the first order model was best fitted with the release profile of baicalin from hydrogel matrix.Besides,MTT assay,AO/EO staining assay as well as hemolytic activity test revealed the excellent cytocompatibility of baicalin/F127 hydrogels.Antioxidant activity assay demonstrated the cytoprotective activity of baicalin/F127 hydrogels against reactive oxygen species(ROS).Furthermore,the in vivo experiments exhibited the ability of baicalin/F127 hydrogel to accelerate wound healing.In conclusion,this novel injectable baicalin/F127 hydrogel should have bright application for chronic wound treatment. | Guiting Liu Ziting Bao Jun Wu | 2020 | Chinese Chemical Letters2020，31，7： | 4 |
| 18 | Recruiting endogenous stem cells: a novel therapeutic approach for erectile dysfunction显示文摘由于他们的再生能力,移植干细胞(SC ) 代表最有希望的方法之一恢复可勃起的机能障碍(编辑) 。然而,不够的来源,侵略过程,道德、规章的问题在临床的应用妨碍他们的使用。在机关和纸巾的内长的 SCs/progenitor 房间居民在成人生活在开发期间并且为织物动态平衡为 organogenesis 起关键作用。甚至没有任何治疗学的干预,人的身体有一个柔韧的 self-healing 能力修理损坏纸巾或机关。因此, SCs-for-ED 治疗不应该被限制到一条供应方面途径。居民内长的 SC 在病人存在能也是为编辑的一个潜在的目标治疗。这评论的目的是总结当代的证据关于:(1 ) SC 壁龛和 SC 在 vitro 的生物特征;(2 ) 内长的 SC 的本地化和动员;(3 ) 存在阴茎内长的 SC 和他们动员的可能的模式的证据。我们在大量基本研究为与这些方面有关的文章在 PubMed 上执行了搜索。一起,众多的证据保持内长的 SC 将是为编辑的治疗的一条新奇治疗学的途径的诺言。 | Zhong-Cheng Xin Yong-De Xu Guiting Lin Tom FLue Ying-Lu Guo | 2016 | Asian Journal of Andrology2016，18，1： | 3 |
| 19 | Tunica albuginea allograft: a new model of LaPeyronie's disease with penile curvature and subtunical ossification显示文摘LaPeyronie 的疾病(PD ) 的 pathophysiology 被认为是 multifactorial,包含愈合的基因倾向,损伤,发炎和改变的创伤。然而,这些因素还没用动物模型被验证了。在这研究,我们介绍了 tunica albuginea allograft 获得的一个新模型。40 的一个总数, 16-week-old 男性老鼠被使用。这些, 8 只老鼠用作控制并且经历了 10 ×有随后的 autografting 的 2-mm-wide tunical 切除,而留下 32 与另一只老鼠的 tunica 与缺点的 grafting 经历了一样的切除。词法、功能测试在 grafting 以后在 1, 3, 7 和 12 个星期被执行。Intracavernous 压力,阴茎弯曲的度和有弹性的纤维长度为在 allograft 和控制组之间的比较被评估。纸巾为组织学的检查被获得。阴茎弯曲作为与控制老鼠相比在 allografted 老鼠是显著地更大的。可勃起的功能在所有老鼠被维持,除了在在 12 个星期估计的那些。弹力素纤维长度作为与控制相比在 allografted tunica 被减少。SMAD2 表示在 allograft 的内部部分被检测,并且 collagen-II- 和 osteocalcin 积极的房间也被注意。在老鼠的 Tunica albuginea (TA ) allograft 是 PD 的一个优秀模型。在 12 个星期和在 TA 的内部层的骨化的存在以后的弯曲的坚持类似于与 PD 在人观察的那些。用这个动物模型的确认研究将为有效治疗学的干预帮助 PD pathophysiology 的理解。 | Ludovic Ferretti Thomas M Fandel Xuefeng Qiu Haiyang Zhang Hazem Orabi Alex K Wu Lia Banie Guifang Wang Guiting Lin Ching-Shwun Lin Tom FLue | 2014 | Asian Journal of Andrology2014，16，4： | 3 |
| 20 | 2D nanomaterials for tissue engineering application显示文摘Recently,tissue engineering has developed into a powerful tool for repairing and reconstructing damaged tissues and organs.Tissue engineering scaffolds play a vital role in tissue engineering,as they not only provide structural support for targeted cells but also serve as templates that guide tissue regeneration and control the tissue structure.Over the past few years,owing to unique physicochemical properties and excellent biocompatibility,various types of two-dimensional(2D)nanomaterials have been developed as candidates for the construction of tissue engineering scaffolds,enabling remarkable achievements in bone repair,wound healing,neural regeneration,and cardiac tissue engineering.These efforts have significantly advanced the development of tissue engineering.In this review,we summarize the latest advancements in the application of 2D nanomaterials in tissue engineering.First,each typical 2D nanomaterial is introduced briefly,followed by a detailed description of its applications in tissue engineering.Finally,the existing challenges and prospects for the future of the application of 2D nanomaterials in tissue engineering are discussed. | Jingyang Zhang Haolin Chen Meng Zhao Guiting Liu Jun Wu | 2020 | Nano Research2020，13，8： | 3 |
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