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| 1 | The late Paleoproterozoic extension event:aulacogens and dyke Swarms in the North China craton显示文摘在北方中国 Craton (NCC ) 的延期结构和构造牵连基于 mafic 塘群和地球年代学在这篇论文被讨论,与 aulacogens 的地球年代学结合。迟了的 Paleoproterozoic 时间是在 NCC 的前寒武纪的进化的重要拐弯处点。延期系统(例如 aulacogens 和塘群) 在 NCC 是普遍的,它与类似于现代的板的僵硬特征标记 NCC 的碳化。当模特儿的 paleostress 地建议塘聚集, aulacogens 是标记超级大陆,不是 synorogenic 的开始的 arogenic 扩展和 postorogenic 扩展。在 NCC 的 mafic 塘群主要从 1.83 ~ 1.77 Ga 。NCC 的延期是很有限的易碎的扩展,平均延期比率仅仅是 0.35% 由 mafic 塘群延期计算给,大多数在 NCC 的扩展被 aulacogens 因此贡献。mafic 塘群在起源与 aulacogens 被联系。 | HOU Guiting LI Jianghai LIU Yulin QIAN Xianglin | 2006 | Progress in Natural Science:Materials International2006，16，2： | 13 |
| 2 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 3 | End Late Paleozoic tectonic stress field in the southern edge of Junggar Basin显示文摘这份报纸论述在由解释压力反应的 Junggar 盆的南部的边的晚古生代的构造压力地组织的结束(女同性恋,褶层,有岩石光滑面的差错并且结合关节) 。最大的主要压力轴的方向被解释是 NWSE (大约 325 牥污 ? 整浲吗? | Wei Ju Guiting Hou Le Li Fangfeng Xiao | 2012 | Geoscience Frontiers2012，3，5： | 5 |
| 4 | A gene that underwent adaptive evolution, LAC2 (LACCASE), in Populus euphratica improves drought tolerance by improving water transport capacity显示文摘Drought severely limits plant development and growth;accordingly,plants have evolved strategies to prevent water loss and adapt to water deficit conditions.However,experimental cases that corroborate these evolutionary processes are limited.The LACCASEs(LACs)family is involved in various plant development and growth processes.Here,we performed an evolutionary analysis of LACs from Populus euphratica and characterized the functions of LACs in Arabidopsis and poplar.The results showed that in PeuLACs,multiple gene duplications led to apparent functional redundancy as the result of various selective pressures.Among them,PeuLAC2 underwent strong positive selection.Heterologous expression analyses showed that the overexpression of PeuLAC2 alters the xylem structure of plants,including thickening the secondary cell wall(SCW)and increasing the fiber cell length and stem tensile strength.Altogether,these changes improve the water transport capacity of plants.The analysis of the physiological experimental results showed that PeuLAC2-OE lines exhibited a stronger antioxidant response and greater drought tolerance than WT.Three genes screened by transcriptome analysis,NAC025,BG1,and UGT,that are associated with SCW synthesis and drought stress were all upregulated in the PeuLAC2-OE lines,implying that the overexpression of PeuLAC2 thickened the SCW,improved the water transport capacity of the plant,and further enhanced its drought tolerance.Our study highlights that genes that have undergone adaptive evolution may participate in the development of adaptive traits in P.euphratica and that PeuLAC2 could be a candidate gene for molecular genetic breeding in trees. | Zhimin Niu Guiting Li Hongyin Hu Jiaojiao Lv Qiwei Zheng Jianquan Liu Dongshi Wan | 2021 | Horticulture Research2021，8，1： | 2 |
| 5 | A novel hydrogel with glucose-responsive hyperglycemia regulation and antioxidant activity for enhanced diabetic wound repair显示文摘Enhanced diabetic wound repair remained a global challenge.Herein,we reported a novel hydrogel with glucose-responsive hyperglycemia regulation and antioxidant activity for enhanced diabetic wound repair.In this study,gallic acid(GA)with strong antioxidant activity was grafted onto chitosan(CS)chains by one-step synthesis,and then incorporated into poly(ethylene glycol)diacrylate(PEG-DA)hydrogel matrix to obtain a novel antioxidant hybrid hydrogel(PEG-DA/CS-GA).Meanwhile,polyethyleneimine(PEI)was modified with a unique glucose-sensitive phenylboronic acid(PBA)molecule to load insulin(PEIPBA/insulin nano-particles,PEI-PBA/insulin NPs),which could be immobilized in the PEG-DA/CS-GA hybrid hydrogel by the formation of dynamic borate bond between the phenylboronic acid groups on the PEI-PBA and the polyphenol groups on the CSGA.The results indicated that the PEG-DA/PEI-PBA/insulin/CS-GA(PPIC)hydrogel platform not only had remarkable biocompatibility,but also displayed extraordinary antioxidant properties(DPPH scavenging rate>95.0%),and effectively protected cells from oxidative damage(decreased MDA levels,increased Superoxide dismutase(SOD)levels and stable GSH/GSSG levels).Meanwhile,the PPIC hydrogel also exhibited unique glucose-responsive insulin release characteristics,and effectively regulated the blood glucose level.The in vitro and in vivo results demonstrated that our PPIC hydrogel could promoted angiogenesis(increased VEGF and CD 31 expression),reshaped the inflammatory microenvironment(decreased IL-6 and increased IL-10 level),and achieved wound closure within 20 days.All these results strongly indicated that the PPIC hydrogel represented a tough and efficient platform for diabetic wound treatment. | Zejun Xu Guiting Liu Qing Li Jun Wu | 2022 | Nano Research2022，15，6： | 2 |
| 6 | Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing显示文摘Base editing, as an expanded clustered regularly interspaced short palindromic repeats(CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus(SaCas9), is important in genome editing because it can edit sites with HHHAAT protospacer adjacent motif(PAM) that the canonical Streptococcus pyogenes Cas9(SpCas9) or its variants(e.g. xCas9, Cas9-NG) cannot. However, several technical parameters of SaKKH involved base editors have not been well defined and this uncertainty limits their application. We developed an effective multiplex cytosine base editor(SaKKHn-pBE) and showed that it recognized NNARRT, NNCRRT, NNGRGT, and NNTRGT PAMs. Based on 27 targets tested, we defined technical parameters of SaKKHn-pBE including the editing window, the preferred sequence context, and the mutation type. The editing efficiency was further improved by modification of the SaKKH sgRNA. These advances can be applied in future research and molecular breeding in rice and other plants. | Chengwei Zhang Feipeng Wang Si Zhao Guiting Kang Jinling Song Lu Li Jinxiao Yang | 2020 | The Crop Journal2020，8，3： | 1 |
| 7 | Identification of an aberrant cell line among human adipose tissue-derived stem cell isolates显示文摘 | Hongxiu Ning Gang Liu Guiting Lin Maurice Garcia Long-Cheng Li Tom F. Lue Ching-Shwun Lin | 2008 | Differentiation2008，，2： | 1 |
| 8 | An automatic bridge detection technique for high resolution SAR images显示文摘 | Guiting Wang Shah Huang Li Chengjiao | | 0，，： | 1 |
| 9 | Low-intensity pulsed ultrasound stimulates proliferation of stem/progenitor cells: what we need to know to translate basic science research into clinical applications显示文摘Low-intensity pulsed ultrasound (LIPUS) is a promising therapy that has been increasingly explored in basic research and clinical applications. LIPUS is an appealing therapeutic option as it is a noninvasive treatment that has many advantages, including no risk of infection or tissue damage and no known adverse reactions. LIPUS has been shown to have many benefits including promotion of tissue healing, angiogenesis, and tissue regeneration;inhibition of inflammation and pain relief;and stimulation of cell proliferation and differentiation. The biophysical mechanisms of LIPUS remain unclear and the studies are ongoing. In recent years, more and more research has focused on the relationship between LIPUS and stem/progenitor cells. A comprehensive search of the PubMed and Embase databases to July 2020 was performed. LIPUS has many effects on stem cells. Studies show that LIPUS can stimulate stem cells in vitro;promote stem cell proliferation, differentiation, and migration;maintain stem cell activity;alleviate the problems of insufficient seed cell source, differentiation, and maturation;and circumvent the low efficiency of stem cell transplantation. The mechanisms involved in the effects of LIPUS are not fully understood, but the effects demonstrated in studies thus far have been favorable. Much additional research is needed before LIPUS can progress from basic science research to large-scale clinical dissemination and application. | Yan Tan Yang Guo Amanda B Reed-Maldonado Zheng Li Guiting Lin Shu-Jie Xia Tom F Lue | 2021 | Asian Journal of Andrology2021，23，6： | 1 |
| 10 | Late Paleoproterozoic extension and a paleostress field reconstruction of the North China Craton显示文摘 | Guiting Hou Chuancheng Wang Jianghai Li Xianglin Qian | 2006 | Tectonophysics2006，，1： | 1 |
| 11 | Late Paleoproterozoic extension and a paleo - stress field reconstruc- tion of the North China Craton 显示文摘 | Guiting Hou Chuancheng Wang Jianghai Li Xianglin Qian | 2006 | Tectonophysics2006，422，14： | 1 |
| 12 | A polyphenol-modified chitosan hybrid hydrogel with enhanced antimicrobial and antioxidant activities for rapid healing of diabetic wounds显示文摘High oxidative stress injury and bacterial infection are the main challenges that impair wound healing in diabetic patients.Therefore,a hydrogel with enhanced antimicrobial and antioxidant properties was developed for rapid healing of diabetic wounds.In this study,chitosan methacrylate-gallic acid(CSMA-GA)polymer with antioxidant activity,antimicrobial activity,and ultraviolet(UV)-triggered gelling properties was developed as a hydrogel precursor.Meanwhile,amphiphilic Pluronic F127 molecules were used to load hydrophobic chlorhexidine drug molecules to obtain F127/chlorhexidine nanoparticle(NP)with strong antibacterial activity.Subsequently,F127/chlorhexidine NPs were encapsulated in CSMA-GA hydrogel to further enhance its antibacterial activity.The hybrid hydrogel platform(CSMA-GA/F127/chlorhexidine(CMGFC))exhibited high antibacterial efficiency(>99.9%)and strong reactive oxygen species(ROS)scavenging ability(>80.0%),which effectively protected cells from external oxidative stress(upregulated superoxide dismutase(SOD)and glutathione/oxidized glutathione disulfide(GSH/GSSG)levels and downregulated malondialdehyde(MDA)levels).Moreover,in vivo results proved that the CMGFC hydrogel significantly reduced inflammatory responses(downregulated interleukin-6(IL-6)and upregulated interleukin-10(IL-10)levels),promoted angiogenesis(upregulated vascular endothelial growth factor(VEGF)and platelet endothelial cell adhesion molecule-1(CD 31)levels),and wound healing(enhanced collagen deposition and tissue remodelling).Overall,the CMGFC hydrogel with enhanced antimicrobial and antioxidant properties demonstrated significant potential to enhance diabetic wound healing. | Zejun Xu Guiting Liu Li Zheng Jun Wu | 2023 | Nano Research2023，16，1： | 0 |
| 13 | Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.显示文摘Sesame Fusarium wilt(SFW),caused by Fusarium oxysporum f.sp.sesami(Fos),is one of the most devastating diseases affecting sesame cultivation.Deciphering the genetic control of SFW resistance is pivotal for effective disease management in sesame.An inheritance study on a cross between the highly resistant variety Yuzhi 11 and the highly susceptible accession Sp1 using a Fos pathogenicity group 1 isolate indicated that resistance was conferred by a single dominant allele.The target locus was located in a 1.24 Mb interval on chromosome 3 using a combination of cross-population association mapping and bulked segregant analysis.Fine genetic mapping further narrowed the interval between 21,350 and 21,401 kb.The locus Sindi_0812400 was identified as the SFW resistance gene and officially designated SiRLK1.This gene encodes a specific malectin/receptor-like protein kinase with three putative tandem kinase domains and is considered a kinase fusion protein.Sequence analysis revealed that a high proportion(49.44%)of variants within the locus was located within the kinase domainⅢ,and several of which were evidently associated with the diversity in SFW response,indicating the critical role of kinase domainⅢin expression of disease resistance.These findings provide valuable information for further functional analysis of SFW resistance genes and marker-assisted resistance breeding in sesame. | Yinghui Duan Wenwen Qu Shuxian Chang Ming Ju Cuiying Wang Cong Mu Hengchun Cao Guiting Li Qiuzhen Tian Qin Ma Zhanyou Zhang Haiyang Zhang Hongmei Miao | 2024 | The Crop Journal2024，12，1： | 0 |
| 14 | L-Arginine based polyester amide/hyaluronic acid hybrid hydrogel with dual anti-inflammation and antioxidant functions for accelerated wound healing显示文摘Nowadays, there are still many challenges to skin regeneration. As a new type of skin substitute, hydrogel has emerging gradually with its excellent properties. However, it is still a challenge to combine with biological active agents to facilitate skin regeneration. Under the circumstance, we synthesized argininebased poly(ester amide)(Arg-PEA) and hyaluronic acid(HA-MA), and combined them into new hybrid hydrogels via photo-crosslinking. We found that the internal structure and physicochemical properties of hybrid hydrogels were greatly improved with the increase of content of Arg-PEA. Therefore, we designed hybrid hydrogels with 5 wt% and 10 wt% of Arg-PEA content, respectively. Besides, we selected the corresponding anti-inflammatory(CRP, TNF-α) indicators to detect the anti-inflammatory properties of the hybrid hydrogels at the protein level, and the corresponding antioxidant indicators(SOD, GSH/GSSG, MDA)were selected to investigate the antioxidant properties of hybrid hydrogels at the cellular level in vitro.In addition, we also selected relevant genes to test the effect of hybrid hydrogels on fibrosis and vascularization in the process of skin wound healing in vitro and verified them in vivo with a mouse dorsum wound model. The results confirmed that Arg-PEA/HA-MA(AH) hybrid hydrogel was a prospective scaffold material for skin regeneration. | Tong Liu Guiting Liu Jianhua Zhang Zhangfan Ding Yike Li Krishna Sigdel Xiaoyi Wang Huixu Xie | 2022 | Chinese Chemical Letters2022，33，4： | 0 |
| 15 | Cretaceous Stress Fields Evolution and Its Geodynamic Implications in Jiaolai Basin, Northern China显示文摘The Tan-Lu fault zone(TLFZ)along the East China continental margin experienced sinistral movement at the beginning of Early Cretaceous(ca.145)due to fast oblique subduction of the Izanagi Plate in the Pacific Ocean.It | LI Jie HOU Guiting | 2016 | Acta Geologica Sinica(English Edition)2016，90，S1： | 0 |
| 16 | Hyaluronic acid-methacrylic anhydride/polyhexamethylene biguanide hybrid hydrogel with antibacterial and proangiogenic functions for diabetic wound repair显示文摘Diabetic wounds lead to a decrease in quality of life and an increase in mortality.Current treatment strategies include preventing bacterial adhesion while improving microcirculation.As a new type of wound dressing that imitates natural skin,hydrogel has gradually emerged with its excellent properties.However,existing hydrogels rarely achieve satisfactory results in promoting wound repair and antibacterial simultaneously.In this case,we prepared methacrylic anhydride chemically modified hyaluronic acid as a hydrogel matrix,added polyhexamethylene biguanide as an antibacterial component,and loaded sodium alginate/salidroside composite microspheres which could sustainably release salidroside and thus promote angiogenesis.Hybrid hydrogel(HAMA/PHMB-Ms)was synthesized via photocrosslinking,and its chemical structure,particle size distribution and microstructure were characterized.The satisfactory antibacterial properties of the HAMA/PHMB(15%)-Ms hydrogel were studied in vitro,and its antibacterial rates against E.coli and S.aureus were 97.85%and 98.56%,respectively.In addition,after demonstrating its good biocompatibility,we verified that the HAMA/PHMB-Ms hydrogel has increased granulation tissue formation,more collagen deposition,more subcutaneous capillary formation,and better wound healing than blank control,HAMA and HAMA/PHMB hydrogel on the back wound model of diabetic mice.The results confirmed that HAMA/PHMB-Ms hydrogel was a promising material for the treatment of the diabetic wounds. | Yike Li Huiyu Zheng Yaxian Liang Ming Xuan Guiting Liu Huixu Xie | 2022 | Chinese Chemical Letters2022，33，12： | 0 |
| 17 | Janus hydrogel with dual antibacterial and angiogenesis functions for enhanced diabetic wound healing显示文摘Anti-infection and neovascularization at the wound site are two vital factors that accelerate diabetic wound healing. However, for a wound healing dressing, the two functions need to work at different sites(inner and outer), giving big challenges for dressing design. In this study, we fabricated a novel sodium alginate/chitosan(SA/CS) Janus hydrogel dressing by the assembly of SA hydrogel loaded with silver nanoparticles(Ag NPs) and CS hydrogel impregnated with L-arginine loaded sodium alginate microspheres(Arg MSs) based on electrostatic interactions to combine the two functions. The outer SA-Ag NP hydrogel could prevent infection while avoiding the deposition of Ag NPs in the wound site, and the inner CS-Arg MS hydrogel on the wound surface could realize the sustained release of L-arginine and promote vascular regeneration. The composition, morphology and swelling/degradation of the SA-Ag NP/CS-Arg MS hydrogel were characterized systematically. L-Arginine release behavior has been tested and SA-Ag NP/CSArg MS hydrogel has been confirmed for excellent biocompatibility. Antibacterial and angiogenesis assays demonstrated the antibacterial and angiogenesis characteristics of the SA-Ag NP/CS-Arg MS hydrogel. Finally, in vivo diabetic wound healing assay demonstrated that the SA-Ag NP/CS-Arg MS hydrogel could significantly accelerate re-epithelialization, granulation tissue formation, collagen deposition and angiogenesis, thereby resulting in enhanced diabetic wound healing。 | Guiting Liu Yuan Zhou Zejun Xu Ziting Bao Li Zheng Jun Wu | 2023 | Chinese Chemical Letters2023，34，4： | 0 |
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