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| 1 | 5-Azacytidine induces changes in electrophysiological properties of human mesenchymal stem cells显示文摘以前,导出髓的干细胞(MSC ) 与不确定的脱氧核糖核酸甲基转移酶禁止者 5-azacytidine 对待的老鼠骨头被报导区分 intocardiomyocytes。现在的学习的目的是在从健康骨髓施主获得的人的单音的原子房间调查类似的区别策略的效率。After1-3 段落,文化为 24 h 暴露于到 6 星期进一步的文化跟随的 5-azacytidine (3 mciroM ) 。药处理没导致 myogenic 标记 MyoD 或心脏的 markersNkx2.5 和 GATA-4 的表示并且没在后续期间产出跳动的房间。在补丁夹钳实验,近似 10-15% 对待,未经治疗的房间展出了 L 类型 Ca (2+) 水流。几乎所有房间表面地出现了修正快速或慢的激活动力学的 K (+) 水流。在与匹配时间的控制相比在 6 星期处理以后加倍的 +60 mV 意味着当前的振幅。对待的房间的 Membranecapacitance 比在在处理以后的 controls2 星期内显著地大并且在 6 个星期以后仍然保持高。为 K (+) 隧道 Kv1.1, Kv1.5, Kv2.1, Kv4.3 和 KCNMA1 并且为 Ca (2+) 隧道 Ca (v) 的 mRNAs 的表示层次 1.2 没被 5-azacytidine 影响。在以前显示出禁止 hMSC 的快速或慢慢地激活的 K (+) 水流的集中的 Treatmentwith 钾隧道块 ers tetraethylammonium 和 clofilium 禁止了这些房间的增长。我们的结果建议尽管有进 cardiomyocytes 的 hMSC 的区别的缺席,有 5-azacytidine 的处理在当前的密度引起了深刻变化。 | Bartosz Balanal Cecilia Nicoletti Ihor Zahanich Eva M Graf Torsten Christ Sabine Boxberger Ursula Ravens | 2006 | Cell Research2006，16，12： | 20 |
| 2 | New aspects in the pathomechanism and diagnosis of the laryngopharyngeal reflux-clinical impact of laryngeal proton pumps and pharyngeal p H metry in extraesophageal gastroesophageal reflux disease显示文摘AIM:To determine the laryngeal H+K+-ATPase and pharyngeal p H in patients with laryngopharyngeal reflux(LPR)-symptoms as well as to assess the symptom scores during PPI therapy.METHODS:Endoscopy was performed to exclude neoplasia and to collect biopsies from the posterior cricoid area(immunohistochemistry and PCR analysis).Immunohistochemical staining was performed with monoclonal mouse antibodies against human H+K+-ATPase.Quantitative real-time RT-PCR for each of the H+K+-ATPase subunits was performed.The p H values were assessed in the aerosolized environment of the oropharynx(Dxp H Catheter) and compared to a subsequently applied combined p H/MII measurement.RESULTS:Twenty patients with LPR symptoms were included.In only one patient,the laryngeal H+K+-ATPase was verified by immunohistochemical staining.In another patient,real-time RT-PCR for each H+K+-ATPase subunit was positive.Fourteen out of twenty patients had pathological results in Dxp H,and 6/20 patients had pathological results in p H/MII.Four patients had pathological results in both functional tests.Nine out of twenty patients responded to PPIs.CONCLUSION:The laryngeal H+K+-ATPase can only be sporadically detected in patients with LPR symptoms and is unlikely to cause the LPR symptoms.Alternative hypotheses for the pathomechanism are needed.The role of pharyngeal p H-metry remains unclearand its use can only be recommended for patients in a research study setting. | Valentin Becker Romina Drabner Simone Graf Christoph Schlag Simon Nennstiel Anna Maria Buchberger Roland M Schmid Dieter Saur Monther Bajbouj | 2015 | World Journal of Gastroenterology2015，21，3： | 14 |
| 3 | 传统DNA疫苗载体与Semliki森林病毒复制子对HIV-1 Pr55^(gag)表达与体液免疫原性的比较性研究显示文摘为探索Semliki森林病毒 (SFV)衍生的复制型DNA载体可否用于HIV疫苗的候选载体 ,对该载体与传统DNA疫苗载体对HIV - 1Pr5 5 gag的表达与体液免疫原性进行了系统比较研究。将野生型 (wtgag)及密码子改造(syngag)的HIV - 1ⅢBgag 基因分别克隆于SFVDNA载体及传统DNA疫苗载体 [pCDNA3 1(+) ],对其Pr5 5 gag细胞内表达水平、Pr5 5 gag病毒样颗粒释放、以及在BALB/c鼠的体液免疫原性进行了比较。在 2 93T、H12 99、C2C12和BHK细胞系中 ,SFV -wtgag可以Rev非依赖方式有效表达Pr5 5 gag ,而 pC -wtgag转染的细胞不能有效表达Pr5 5 gag,从而不能诱导小鼠产生免疫反应。虽然SFV质粒的细胞转化效率明显低于pCDNA载体 ,SFV -wtgag和SFV -syngag在细胞内Pr5 5 gag的表达量与 pC -syngag相似 ,而Pr5 5 gag病毒样颗粒的释放明显低于 pC -syngag。在肌内注射免疫的小鼠中 ,低剂量 (0 1和 1 0 μg)的SFV及 pCDNAgag表达质粒均未诱导出GAG特异性免疫反应。在高剂量 (10 ,30 ,10 0 μg)免疫组中 ,与SFVgag表达质粒相比 ,pC -syngag可诱导出较高水平的TH1型GAG特异性抗体。SFV -syngag较SFV -wtgag可诱导出高水平的体液免疫反应。结果提示 ,SFV衍生的复制子单独使用不能在小鼠诱导出优于传统DNA疫苗载体的HIV - 1GAG特异性? | 张健慧 Jens Wild Kurt Bieler Marcus Graf Ludwig Deml Hans Wolf PeterLiljestrm Ralf Wagner 邵一鸣 | 2002 | 病毒学报2002，18，1： | 13 |
| 4 | 皮革中痕量六价铬的形成原因及其检测办法显示文摘指出了六价铬离子的危害及其检测方法 。 | Dieter Graf 刘显奎 | 2001 | 中国皮革2001，30，23： | 11 |
| 5 | Differential treatment and early outcome in the interventional endoscopic management of pancreatic pseudocysts in 27 patients显示文摘瞄准:胰腺的假包囊(PPC ) 仅仅在腹的疼痛的情况中作为胰腺炎的复杂并发症被接近,感染,流血,并且压缩到胃肠道或胆汁的树上。方法:从 02/01/2002 到 05/31/2004,经历了 interventional 的有征兆的 PPC 的所有连续病人内视镜的途径在这飞行员盒子系列研究被评估:组(Gr ) 我主要经皮(外部) ,指导超声的排水。Gr。II 主要的指导 EUS 的胰囊肿胃吻合术。Gr。包括 intracystic necrosectomy 的 III-EUS-guided 胰囊肿胃吻合术。结果:( = “列在后面在上面”:n = 27 ) :Gr。我(n = 9;33.3%) :没有抱怨(n = 3 ) ;变化一进内部排水外部(n = 4 ) ;复杂并发症:(a) 流血(n = 1 ) 在 ICU 由 3 d 列在后面,在 40 d 以后的分泌物;(b) 腐败吃惊(n = 1 ) 由 ICU 和几剖腹术列在后面规划洗室年龄和 necrosectomy,在 74 d 以后的死亡。Gr。II (n = 13;48.1%) :没有抱怨(n = 11 ) ;外部排水(n = 2 ) ;从 13 个盒子的复杂并发症 / 问题:第二分开的假包囊(n = 1 ) 与外部排水(自从与主要内部排水的没有通讯) ;剩余包囊的感染(n = 1 )+ 追随者外部排水;自发的 PPC 穿孔(n = 1 ) 用片断并且随后的胰囊肿胃吻合术的开始的 + 追随者闭合为 2 d 的 ICU。Gr。III (n = 5;18.5%) :在所有病人的没有抱怨,在要求的一般水准二内视镜的过程(变化, 2-6 ) 。结论:胰腺的假包囊的 Interventional 内视镜的管理关于复杂并发症率(11.1%) 和死亡(3.7%) 是有与外科和可接受的结果相比的低侵略海角的一种合理其他的治疗选择,作为由这些证明起始的学习结果。 | Uwe Will Conrad Wegener Kai-Ivo Graf Igor Wanzar Thomas Manger Frank Meyer | 2006 | World Journal of Gastroenterology2006，12，26： | 9 |
| 6 | Metastasized pancreatic carcinoma with neoadjuvant FOLFIRINOX therapy and R0 resection显示文摘Patients with metastasized carcinoma of the pancreas have a very poor prognosis, and long-term survival cannot be expected. This case report describes two patients with an initial diagnosis of metastatic pancreatic cancer, both with hepatic metastases and one with an additional peritoneal carcinomatosis. Initially, both patients were treated intravenously with the FOLFIRINOX chemotherapy regimen, consisting of 5-FU, folinic acid, irinotecan and oxaliplatin. Surprisingly, the FOLFIRINOX treatment resulted in complete resolution of the hepatic metastases in both patients, with no lesions detectable by computed tomography scan. Furthermore, treatment response included decreased diameter of the primary tumor in the tail of the pancreas and disappearance of the additional peritoneal carcinomatosis. Both patients were discussed by our multidisciplinary tumor board, which recommended surgical resections of the carcinoma. The R0 resection of the primary tumor was successful in both cases and, interestingly, the resected tissues showed no evidence of the hepatic metastases intraoperatively. In the first case, the patient received a postoperative 6-mo course of adjuvant chemotherapy with gemcitabine. In the second case, the patient continued to receive the FOLFIRINOX regimen for an additional 6 mo postoperatively. At 12 mo after the operation, a nonresectable retroperitoneal lymph node metastasis was detected in the first patient, whereas the second patient remained in complete remission at the time of this report(5 mo after the adjuvant therapy was discontinued). This case report is the first of its kind to describe two cases of hepatic metastatic pancreatic carcinoma that were resectable following treatment with FOLFIRINOX. Further studies are required to examine the role of FOLFIRINOX as a neoadjuvant treatment option in subgroups of patients with initially metastasized pancreatic carcinoma. | Sophie Schneitler Patric Krpil Jasmin Riemer Gerald Antoch Wolfram Trudo Knoefel Dieter Hussinger Dirk Graf | 2015 | World Journal of Gastroenterology2015，21，20： | 9 |
| 7 | Innovative immunohistochemistry identifies MMP-9 expressing macrophages at the invasive front of murine HCC显示文摘AIM:To investigate the proteolytic contribution of tumor-associated macrophages(TAM)in tumor invasion,we analyzed whether TAM at the invasive front of small HCC in Abcb4-/--mice show an enhanced expression of MMP-9. METHODS:Liver cryosections of the hepatocellular carcinoma(HCC)invasive front from 12 mo old Abcb4-/--mice were stained for collagen typeⅠand MMP-9 using Alexa488 and Alexa568 labeled secondary antibodies.Afterwards,the Alexa568 dye was bleached and the macrophage marker F4/80 was visualized using Alexa568 labeled secondary antibodies.Finally, photographs of the invasive tumor front were digitally overlaid and analyzed. RESULTS:After complete bleaching of the primary dye,specific fluorescence staining of a third antigen, here F4/80,was successfully performed on the same histological section.With this method,we were able to identify conglomerates of matrix metalloproteinase (MMP-9)expressing macrophages within the tumor capsule of HCC. CONCLUSION:MMP-9 expressing macrophages are involved in matrix remodelling at the invasive tumor front of HCC.The described staining protocol provides a simple yet powerful extension of conventional immuno-histochemistry,facilitating visualization of at least three different antigens plus nuclei in one single histological section. | Martin Roderfeld Timo Rath Frank Lammert Christian Dierkes Jürgen Graf Elke Roeb | 2010 | World Journal of Hepatology2010，2，5： | 6 |
| 8 | 硅烷偶联剂对玻璃及铝胶接耐久性的影响显示文摘硅烷偶联剂被广泛用于胶粘剂与被粘物界面以提高其耐久性。用APES及GPMS硅烷偶联剂 ,采用了诸如混合法、浸入法等方法 ,并对玻璃 -玻璃 ,铝 -铝胶接接头的耐久性进行了研究。结果表明 :偶联剂可以显著提高胶接接头的耐久性。 | W Brockmann S Emrich M Graf 曲军 | 2000 | 粘接2000，21，1： | 5 |
| 9 | Reduced expression ofβ-catenin inhibitor Chibby in colon carcinoma cell lines显示文摘AIM: To analyse the Chibby expression and its function in colon carcinoma cell lines and colorectal carcinoma (CRC). METHODS: Chibby expression levels were investigated by quantitative RT-PCR in a panel of seven different colon carcinoma cell lines. By sequencing, we analysed mutational status of Chibby. To test whether Chibby exhibited effects onβ-catenin signalling in colon carcinoma cells, we transfected SW480 cells with Chibby expression plasmid and, subsequently, analysed activity of p-catenin and tested for alterations in cellular phenotype. In addition, we examined Chibby mRNA levels in samples of colorectal carcinomas and adjacent normal tissues by using quantitative RT-PCR and hybridised gene chips with samples from CRC and normal tissues. RESULTS: Chibby mRNA expression was strongly down-regulated in colon carcinoma cell lines in comparison to normal colon epithelial cells and no mutation in any of the examined colon carcinoma cell lines was found. Further, we could show that Chibby inhibited p-catenin activity in TOPflash assays when over-expressed in SW480 cells. Proliferation and invasion assays with Chibby transfected SW480 cells did not reveal profound differences compared to control cells. In contrast to these in vitro data, quantitative RT-PCR analyses of Chibby mRNA levels in CRC tumor samples did not show significant differences to specimens in adjacent non-cancerous tissue. Consistent with these findings, gene chips analysing tissue samples of tumors and corresponding normal tissue did not show altered Chibby expression CONCLUSION: Altered Chibby expression might be observed in vitro in different colon carcinoma cell lines. However, this finding could not be confirmed in vitro in CRC tumors, indicating that Chibby is not likely to promote CRC tumor development or progression. As Chibby is an important inhibitor ofβ-catenin signalling, our data implicate that the usability of colon carcinoma cell lines for in vitro studies analysing the Wnt/β-catenin pathway in colorectal carcinoma needs extensive verification. | Marion M Schuierer Elisabeth Graf Ken-Ichi Takemaru Wolfgang Dietmaier Anja-Katrin Bosserhoff | 2006 | World Journal of Gastroenterology2006，12，10： | 5 |
| 10 | The Clavien-Dindo Classification of Surgical Complications: Five-Year Experience显示文摘 | Pierre A. Clavien Jeffrey Barkun Michelle L. de Oliveira Jean Nicolas Vauthey Daniel Dindo Richard D. Schulick Eduardo de Santiba?es Juan Pekolj Ksenija Slankamenac Claudio Bassi Rolf Graf René Vonlanthen Robert Padbury John L. Cameron Masatoshi Makuuchi | 2009 | Annals of Surgery2009，，2： | 4 |
| 11 | Safety, tolerability, and antibody response of active Aβ immunotherapy with CAD106 in patients with Alzheimer’s disease: randomised, double-blind, placebo-controlled, first-in-human study显示文摘 | Bengt Winblad Niels Andreasen Lennart Minthon Annette Floesser Georges Imbert Thomas Dumortier R Paul Maguire Kaj Blennow Joens Lundmark Matthias Staufenbiel Jean-Marc Orgogozo Ana Graf | 2012 | Lancet Neurology2012，，7： | 3 |
| 12 | Paving the Way to Open Data显示文摘It is easy to argue that open data are critical to enabling faster and more effective research discovery.In this article,we describe the approach we have taken at Wiley to support open data and to start enabling more data to be FAIR data(Findable,Accessible,Interoperable and Reusable)with the implementation of four data policies:“Encourages”,“Expects”,“Mandates”and“Mandates and Peer Reviews Data”.We describe the rationale for these policies and levels of adoption so far.In the coming months we plan to measure and monitor the implementation of these policies via the publication of data availability statements and data citations.With this information,we’ll be able to celebrate adoption of data-sharing practices by the research communities we work with and serve,and we hope to showcase researchers from those communities leading in open research. | Yan Wu Elizabeth Moylan Hope Inman Chris Graf | 2019 | Data Intelligence2019，1，4： | 3 |
| 13 | Hypothermic oxygenated perfusion (HOPE) protects from biliary injury in a rodent model of DCD liver transplantation显示文摘 | Andrea Schlegel Rolf Graf Pierre-Alain Clavien Philipp Dutkowski | 2013 | Journal of Hepatology2013，，： | 3 |
| 14 | 植酸的用途显示文摘许多植物种子含有1-3%的植酸(六磷酸肌醇酯),植酸有很强的螯合能力,并能影响如Ca^(2+)、Zn^(2+)、Fe^(3+)等多阳离子营养因子的吸收,这已是几十年来的重要研究课题。然而,在美国文献中还几乎没有植酸的其它化学特性及充分利用方面的资料。本综述概括了植酸在当今医药、牙科、营养和工业中的应用,并对这种廉价、易得的化学物质提出另外一些新的用途。 | 〔美国〕ERNST GRAF 王林风(译) 王以群(校) | 1986 | 中国油脂1986，11，2： | 3 |
| 15 | Assessing the impact of the maritime silk road显示文摘The plans for the Maritime Silk Road affect the existing liner services.The purpose of this paper is to quantify using the terms of network theory the impact of these plans and to project the effects on the fleet,utilization,port throughput,and carbon footprint.The analysis is based on publicly available information and reasonable fleet deployment assumptions.It also considers current and alternative routing schedules from Shanghai to Europe.The impact of existing and expected emission control areas(ECAs)along the port chains is also considered. | Orestis Schinas Arnd Graf von Westarp | 2017 | Journal of Ocean Engineering and Science2017，2，3： | 3 |
| 16 | Coupling of crop assignment and vehicle routing for harvest planning in agriculture显示文摘A method for harvest planning based on the coupling of crop assignment with vehicle routing is presented.Given a setting with multiple fields,a path network connecting these,multiple depots at which a number of harvesters are initially located,the main question addressed is:Which crop out of a set of different crops to assign to each field when accounting for the given setting?It must be answered by every farm manager at the beginning of every work-cycle starting with plant seeding and ending with harvesting.Rather than solving an assignment problem only,it is here also accounted for the connectivity between fields.In practice,fields are often located distant apart.Traveling costs of machinery and limited harvesting windows demand optimized operation and route planning.Therefore,the proposed method outputs crop assignments to fields and simultaneously determines crop-tours,i.e.,optimized sequences in which to service fields of the same crop during harvest.It is of particular relevance for larger farms and groups of farms that collaborate and share machinery.Integer programming based exact algorithms are derived.For large numbers of fields,where these algorithms may not be tractable due to computational constraints,elements of clustering and the solution of local Traveling Salesman Problems are added,thereby on the one hand rendering the method heuristic and in general suboptimal,but on the other hand maintaining large-scale applicability. | Mogens Graf Plessen | 2019 | Artificial Intelligence in Agriculture2019，，2： | 3 |
| 17 | EXPERIMENTS ON FLOW AROUND A CYLINDER IN A SCOURED CRANNEL BED显示文摘The flow pattern around a cylinder, installed in a scourd channel bed, was experimentally investigated. Detailed measurements of the instantaneous 3D velocities were performed by using an Acoustic Doppler Velocity Profiler (ADVP), from which the profiles of the time^averaged velocities and turbulence stresses were obtained. It is shown that the influence of thC cylinder and of the scour hole alters the approach flow; this is essentially confined to the viciulty of the cylinder and to the inside of the scour hole. The horseshoe vortex is measured as a flow reversal inside the scour hole, formed by the downward flow along the cylinder face and the reversed flow at the scour bed. | I. ISTIARTO and W.H. GRAF 1 Res. Asst., Lab. de recherches hydrauliques, Ecole Polytechnique Federale, Lausanne, Switzerland. E-mail: adhy.kurniawan@epfl.ch or istiarto@tsipil.ugm.ac.id 2 Prof, Lab. de recherches hydrauliques, Ecole Polytechnique Federal | 2001 | International Journal of Sediment Research2001，16，4： | 3 |
| 18 | 应用适当MRI作为依据对幼年和成年膝关节剥脱性骨软骨炎不稳定性进行诊断显示文摘目的回顾性分析前述MRI诊断标准检测幼年和成年膝关节剥脱性骨软骨炎(osteochondritis dissecans,OCD)不稳定性的敏感性和特异性,并与关节镜所见进行对照研究。材料与方法本研究符合HIPAA要求,并经学术审查委员会批准免除知情同意书。本研究包括32例骨骼未成熟病人(男25例,女7例,平均年龄14.4岁)的36个幼年性膝关节OCD,33例骨骼成熟病人(男25例,女8例,平均年龄26.2岁)的34个膝关节成年性OCD。所有病人均经MRI和关节镜检查。由2名放射医师回顾性分析MRI检查,在意见一致的条件下确定是否存在前述MR成像条件下不稳定OCD的表现(即高T2信号强度边缘,周围囊变, | R. Kijowski D.G. Blankenbaker K. Shinki J.P. Fine B.K. Graf A.A. DeSmet. 白荣杰(译) 唐光健(校) | 2008 | 国际医学放射学杂志2008，31，5： | 3 |
| 19 | Ultrastructural findings after intraarticular application of hyaluronan in a canine model of arthropathy显示文摘 | Wenz W Breusch SJ Graf J | | Journal of Orthopaedic Research0，，： | 2 |
| 20 | Suppression of colonic cancer by dietary phytic acid 显示文摘 | Graf E Eaton JW | 1993 | Nutr Cancer1993，19，： | 2 |
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