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| 1 | 5-Azacytidine induces changes in electrophysiological properties of human mesenchymal stem cells显示文摘以前,导出髓的干细胞(MSC ) 与不确定的脱氧核糖核酸甲基转移酶禁止者 5-azacytidine 对待的老鼠骨头被报导区分 intocardiomyocytes。现在的学习的目的是在从健康骨髓施主获得的人的单音的原子房间调查类似的区别策略的效率。After1-3 段落,文化为 24 h 暴露于到 6 星期进一步的文化跟随的 5-azacytidine (3 mciroM ) 。药处理没导致 myogenic 标记 MyoD 或心脏的 markersNkx2.5 和 GATA-4 的表示并且没在后续期间产出跳动的房间。在补丁夹钳实验,近似 10-15% 对待,未经治疗的房间展出了 L 类型 Ca (2+) 水流。几乎所有房间表面地出现了修正快速或慢的激活动力学的 K (+) 水流。在与匹配时间的控制相比在 6 星期处理以后加倍的 +60 mV 意味着当前的振幅。对待的房间的 Membranecapacitance 比在在处理以后的 controls2 星期内显著地大并且在 6 个星期以后仍然保持高。为 K (+) 隧道 Kv1.1, Kv1.5, Kv2.1, Kv4.3 和 KCNMA1 并且为 Ca (2+) 隧道 Ca (v) 的 mRNAs 的表示层次 1.2 没被 5-azacytidine 影响。在以前显示出禁止 hMSC 的快速或慢慢地激活的 K (+) 水流的集中的 Treatmentwith 钾隧道块 ers tetraethylammonium 和 clofilium 禁止了这些房间的增长。我们的结果建议尽管有进 cardiomyocytes 的 hMSC 的区别的缺席,有 5-azacytidine 的处理在当前的密度引起了深刻变化。 | Bartosz Balanal Cecilia Nicoletti Ihor Zahanich Eva M Graf Torsten Christ Sabine Boxberger Ursula Ravens | 2006 | Cell Research2006，16，12： | 20 |
| 2 | New aspects in the pathomechanism and diagnosis of the laryngopharyngeal reflux-clinical impact of laryngeal proton pumps and pharyngeal p H metry in extraesophageal gastroesophageal reflux disease显示文摘AIM:To determine the laryngeal H+K+-ATPase and pharyngeal p H in patients with laryngopharyngeal reflux(LPR)-symptoms as well as to assess the symptom scores during PPI therapy.METHODS:Endoscopy was performed to exclude neoplasia and to collect biopsies from the posterior cricoid area(immunohistochemistry and PCR analysis).Immunohistochemical staining was performed with monoclonal mouse antibodies against human H+K+-ATPase.Quantitative real-time RT-PCR for each of the H+K+-ATPase subunits was performed.The p H values were assessed in the aerosolized environment of the oropharynx(Dxp H Catheter) and compared to a subsequently applied combined p H/MII measurement.RESULTS:Twenty patients with LPR symptoms were included.In only one patient,the laryngeal H+K+-ATPase was verified by immunohistochemical staining.In another patient,real-time RT-PCR for each H+K+-ATPase subunit was positive.Fourteen out of twenty patients had pathological results in Dxp H,and 6/20 patients had pathological results in p H/MII.Four patients had pathological results in both functional tests.Nine out of twenty patients responded to PPIs.CONCLUSION:The laryngeal H+K+-ATPase can only be sporadically detected in patients with LPR symptoms and is unlikely to cause the LPR symptoms.Alternative hypotheses for the pathomechanism are needed.The role of pharyngeal p H-metry remains unclearand its use can only be recommended for patients in a research study setting. | Valentin Becker Romina Drabner Simone Graf Christoph Schlag Simon Nennstiel Anna Maria Buchberger Roland M Schmid Dieter Saur Monther Bajbouj | 2015 | World Journal of Gastroenterology2015，21，3： | 14 |
| 3 | 硅烷偶联剂对玻璃及铝胶接耐久性的影响显示文摘硅烷偶联剂被广泛用于胶粘剂与被粘物界面以提高其耐久性。用APES及GPMS硅烷偶联剂 ,采用了诸如混合法、浸入法等方法 ,并对玻璃 -玻璃 ,铝 -铝胶接接头的耐久性进行了研究。结果表明 :偶联剂可以显著提高胶接接头的耐久性。 | W Brockmann S Emrich M Graf 曲军 | 2000 | 粘接2000，21，1： | 5 |
| 4 | Reduced expression ofβ-catenin inhibitor Chibby in colon carcinoma cell lines显示文摘AIM: To analyse the Chibby expression and its function in colon carcinoma cell lines and colorectal carcinoma (CRC). METHODS: Chibby expression levels were investigated by quantitative RT-PCR in a panel of seven different colon carcinoma cell lines. By sequencing, we analysed mutational status of Chibby. To test whether Chibby exhibited effects onβ-catenin signalling in colon carcinoma cells, we transfected SW480 cells with Chibby expression plasmid and, subsequently, analysed activity of p-catenin and tested for alterations in cellular phenotype. In addition, we examined Chibby mRNA levels in samples of colorectal carcinomas and adjacent normal tissues by using quantitative RT-PCR and hybridised gene chips with samples from CRC and normal tissues. RESULTS: Chibby mRNA expression was strongly down-regulated in colon carcinoma cell lines in comparison to normal colon epithelial cells and no mutation in any of the examined colon carcinoma cell lines was found. Further, we could show that Chibby inhibited p-catenin activity in TOPflash assays when over-expressed in SW480 cells. Proliferation and invasion assays with Chibby transfected SW480 cells did not reveal profound differences compared to control cells. In contrast to these in vitro data, quantitative RT-PCR analyses of Chibby mRNA levels in CRC tumor samples did not show significant differences to specimens in adjacent non-cancerous tissue. Consistent with these findings, gene chips analysing tissue samples of tumors and corresponding normal tissue did not show altered Chibby expression CONCLUSION: Altered Chibby expression might be observed in vitro in different colon carcinoma cell lines. However, this finding could not be confirmed in vitro in CRC tumors, indicating that Chibby is not likely to promote CRC tumor development or progression. As Chibby is an important inhibitor ofβ-catenin signalling, our data implicate that the usability of colon carcinoma cell lines for in vitro studies analysing the Wnt/β-catenin pathway in colorectal carcinoma needs extensive verification. | Marion M Schuierer Elisabeth Graf Ken-Ichi Takemaru Wolfgang Dietmaier Anja-Katrin Bosserhoff | 2006 | World Journal of Gastroenterology2006，12，10： | 5 |
| 5 | Eval- uation of visual analog scales for the assessment of symptom severity in allergic rhinoconjunctivitis 显示文摘 | SENTI G VAVRICKA B M GRAF N | 2007 | Ann Allergy Asthma Immunol2007，98，： | 1 |
| 6 | Matrix metalloproteinases in inflammatory bowel disease-from basic research to clinical significance显示文摘 | Rath T Roderfeld M Graf J | 2009 | ZGastroenterol2009，47，8： | 1 |
| 7 | Serotonin promotes tumor growth in human hepatocellular cancer显示文摘 | SOLL C JANG J H RIENER M O MORITZ W WILD P J GRAF R | 2010 | Hepatology2010，51，： | 1 |
| 8 | Preference evaluation of foods prepared with imitation cheeses显示文摘 | Lindsay R C Hargett S M Graf T F | 1980 | Food Products Development1980，14，： | 1 |
| 9 | A dynamic lattice Monte Carlo model of ion transport in inhomogeneous dielectric environments: Method and implementation 显示文摘 | Graf P Nitzan A Kumikova M G | 2000 | J Phys Chem B2000，10，： | 1 |
| 10 | Production and purification of N-terminal half-transferrin in Pichia pastoris显示文摘 | STEINLEIN L M GRAF T N IKEDA R A | 1995 | Protein Expr Purif1995，6，5： | 1 |
| 11 | Differences in cardiotoxicity of bupivacaine and ropivacaine are the result of physicochemical and stereos-elective properties显示文摘 | Graf B M Abraham I Eberbach N | 2002 | Anesthesiology2002，96，6： | 1 |
| 12 | The role of action representation in visual object显示文摘 | Helbig H B Graf M Kiefer M | | 0，，： | 1 |
| 13 | Impact of trophblast pene- tration through the basal membrance on the efficacy of drug therapy in tubal pregnancy 显示文摘 | KLEIN M GRAF A KISS A | 1995 | Hum Reprod1995，10，2： | 1 |
| 14 | Fluorescent protein-cell labeling and its application in time-lapse analysis of hematopoietic differentiation显示文摘 | Stadtfeld M Varas F Graf T | 2005 | Methods Mol Med2005，105，： | 1 |
| 15 | The annual volcanic gas input into the atmosphere, in particular into the stratosphere a global data set for the past 100 years显示文摘 | Halmer M M Schmincke H U Graf H F | 2002 | Journal of Volcanology and Geothermal Research2002，115，： | 1 |
| 16 | Cutting edge:CD28-mediated transcriptional and posttranscriptional regulation of IL-2 expression are controlled through different signaling pathways显示文摘 | Sanchez-Lockhart M Marin E Graf EB | 2004 | J Immunol2004，173，12： | 1 |
| 17 | Coal solubility and swelling: 3 A model for coal swelling显示文摘 | PAINTER P C GRAF J COLEMAN M M | 1990 | Energy Fuels1990，4，4： | 1 |
| 18 | Tumor-infiltrating, myeloid-derived suppressor cells inhibit T cell activity by nitric oxide production in an intracranial rat glioma + vaccination model 显示文摘 | Jia W Jackson-Cook C Graf M R | 2010 | J Neuroimmunol2010，223，12： | 1 |
| 19 | Statin treatment after onset of sepsis in a murine model improves survival显示文摘 | Merx M W Liehn E A Graf J | 2005 | Circulation2005，112，1： | 1 |
| 20 | Lattice dynamics and the high-pressure equation of state of Au显示文摘 | Creeff C W Graf M J | 2004 | Phys Rev B2004，69，05： | 1 |
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