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| 1 | Coronary Anomalies: Incidence, Pathophysiology, and Clinical Relevance显示文摘 | Paolo Angelini José Antonio Velasco Scott Flamm | 2002 | Circulation: Journal of the American Heart Association2002，，20： | 4 |
| 2 | Chimeric protein probes for C5a receptors through fusion of the anaphylatoxin C5a core region with a small-molecule antagonist显示文摘Blockade of the interaction of anaphylatoxin C5a with its receptor C5aR1 has been actively studied as a potential treatment for many inflammatory diseases;but current C5a antagonists exhibit inadequate potency and poor species cross-reactivity, and novel biochemical tools are needed to investigate whether the core region of C5a contains important interaction epitopes that can explain these limitations. Herein, we report the development of chimeric protein C5a probes containing both the complete core region of rat or human C5a, and the small-molecule antagonist PMX53-1. These probes were chemically synthesized through hydrazide-based native chemical ligation of a linear peptide hydrazide with the requisite cyclopeptidic antagonist, both of which were made by solid-phase synthesis. Quasi-racemic X-ray crystallography established that attachment of PMX53-1 did not affect the structure of the core region of C5a. Subsequent C5aR1 activity assays demonstrated the probes can provide valuable insights into the development of C5a antagonists;for example, they exhibited significantly better binding affinity and much improved species cross-reactivity than PMX53-1, supporting the notion that the effect of some epitopes outside the C-terminus of C5a should be taken into consideration when designing better C5a antagonists. Surprisingly, the core region of C5a was found to partially agonize C5aR1, suggesting the presence of more than one agonistic interaction in the binding of C5a to C5aR1. This study exemplifies the value of chemical protein synthesis in developing novel receptor probes for drug discovery research. | Chao Zuo Wei-Wei Shi Xiao-Xu Chen Marie Glatz Bernd Riedl Ingo Flamme Elisabeth Pook Jiawei Wang Ge-Min Fang Donald Bierer Lei Liu | 2019 | Science China Chemistry2019，62，10： | 2 |
| 3 | Low NOx combustion technologies for high temperature applications显示文摘 | FLAMME M | 2001 | Energy Conversion and Management2001，42，： | 2 |
| 4 | Boceprevir for Chronic HCV Genotype 1 Infection in Patients with Prior Treatment Failure to Peginterferon/Ribavirin, including Prior Null Response显示文摘 | John M. Vierling Mitchell Davis Steven Flamm Stuart C. Gordon Eric Lawitz Eric M. Yoshida Joseph Galati Velimir Luketic Jonathan McCone Ira Jacobson Patrick Marcellin Andrew J. Muir Fred Poordad Lisa D. Pedicone Janice Albrecht Clifford Brass Anita Y.M. H | 2013 | Journal of Hepatology2013，，： | 2 |
| 5 | ACCF/ACR/AHA/NASCI/SCMR 2010 Expert Consensus Document on Cardiovascular Magnetic Resonance显示文摘 | W. Gregory Hundley David A. Bluemke J. Paul Finn Scott D. Flamm Mark A. Fogel Matthias G. Friedrich Vincent B. Ho Michael Jerosch-Herold Christopher M. Kramer Warren J. Manning Manesh Patel Gerald M. Pohost Arthur E. Stillman Richard D. White Pamela K. Wo | 2010 | Journal of the American College of Cardiology2010，，23： | 1 |
| 6 | Summary of linezolid activity and resistance mechanisms detected during the 2012 LEADER surveillance program for the United States显示文摘 | MENDES RE FLAMM RK HOGAN PA | 2014 | Antimicrob Agents Chemother2014，58，2： | 1 |
| 7 | Antimicrobial susceptibility of Gram-negative organisms isolated from patients hospitalized in intensive care units in United States and European hospitals(2009-2011)显示文摘 | SADER HS FARRELL DJ FLAMM RK | 2014 | Diagn Microbiol Infect Dis2014，78，4： | 1 |
| 8 | Shape control of large lightweight mirrors with dielectric elastomer actuation 显示文摘 | Kornbluh RD Flamm DS Prahlad H | 2003 | SPIE Smart structures and materials 2003:Electroactive polymer actuators and devices (EAPAD)2003，5051，： | 1 |
| 9 | A phase Ⅰ trial of naloxone treatment in acute spinal cord injury显示文摘 | Flamm ES Young W Collins WF | 1985 | Neurosurg1985，63，： | 1 |
| 10 | Intramural hematoma of the thoracic aorta:MR image findings and their prognostic implications显示文摘 | Murray JG Manisali M Flamm SD | 1997 | Radiology1997，204，： | 1 |
| 11 | Validation of real-time three-dimensional echocardiography for quantifying left ventricular volumes in the presence of a left ventricular aneurysm: in vitro and in vivo studies显示文摘 | Jian Xin Qin Michael Jones Takahiro Shiota Neil L Greenberg Hiroyuki Tsujino Michael S Firstenberg Pankaj C Gupta Arthur D Zetts Yong Xu Jing Ping Sun Lisa A Cardon Jill A Odabashian Scott D Flamm Richard D White Julio A Panza James D Thomas | 2000 | Journal of the American College of Cardiology2000，，3： | 1 |
| 12 | Ecosystem Management with MultipleOwners: Landscape Dynamics in a Southern Appalachian Watershed 显示文摘 | Wear D N Turner M G Flamm R 0 | 1996 | Ecological Applications1996，，6： | 1 |
| 13 | Cardiac MRI in the Assessment of Cardiac Injury and Toxicity From Cancer Chemotherapy: A Systematic Review显示文摘 | Paaladinesh Thavendiranathan Bernd J. Wintersperger Scott D. Flamm Thomas H. Marwick | 2013 | Circulation: Cardiovascular Imaging2013，，： | 1 |
| 14 | Role of MRI in clinical cardiology 显示文摘 | Constantine G Shan K Flamm S D | 2004 | Lancet2004，363，9427： | 1 |
| 15 | AGA technical review on the evaluation of liver chemistry tests显示文摘 | Green RM Flamm S | 2002 | Gastroenterology2002，123，： | 1 |
| 16 | Impaired Th2 development and increased mortality during Schistosoma mansoni infection in the absence of CD40/CD154 interaction 显示文摘 | MacDonald AS Patton EA La Flamme AC | 2002 | J Immunol2002，168，9： | 1 |
| 17 | Evidence-basedassessment:patient,procedure,or operator factors asso-ciated with ERCP complications显示文摘 | Aronson N Flamm CR Bohn RL et a1 | 2002 | Gastrointest Endosc2002，566，： | 1 |
| 18 | Once a cesarean, always a controversy显示文摘 | Flamm BL | 1997 | Obstet Gynecol1997，90，2： | 1 |
| 19 | Neoadjuvant Chemotherapy (MVAC) in Locally Invasive Bladder Cancer显示文摘 | SAGASTER J FLAMM M FLAMM | 1996 | EurJ Cancer1996，32，8： | 1 |
| 20 | Clinical diagnosis and man- agement of large vessel vasculitis:Takayasu Arteritis显示文摘 | ChatteijeeS Flamm SD5Tan CD | 2014 | Curr Cardi- ol Rep2014，16，7： | 1 |
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