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| 1 | 利用RNA干扰技术抑制水稻淀粉极限糊精酶基因表达显示文摘构建了含极限糊精酶基因片段反向重复结构的RNAi载体,抑制水稻籽粒中极限糊精酶基因表达获得了极限糊精酶基因功能缺失突变体。经酶切及序列测定证实,成功地构建了胚乳特异表达启动子启动的水稻极限糊精酶基因反向重复结构RNAi载体;通过根癌农杆菌(Agrobacterium tumefaciens)介导水稻(Oryza sativa sp.japonica)转化,经PCR及Southern杂交检测,获得28个水稻转基因再生株系;以极限糊精酶抗体对转基因株系胚乳发育期籽粒蛋白质进行Western杂交,并经SDS-PAGE极限糊精酶活性检测,结果显示,该反向重复结构在胚乳发育期表达,减少了极限糊精酶的积累,获得了不同沉默效果的转基因株系。转化株系012的极限糊精酶活性只有野生型的10%,表明该载体可有效地抑制水稻胚乳中极限糊精酶的活性。 | 黄冰艳 李忠谊 吉万全 海燕 Fleming J Morell M Rahman S | 2007 | 农业生物技术学报2007，15，1： | 13 |
| 2 | Global, regional, and national prevalence of overweight and obesity in children and adults during 1980–2013: a systematic analysis for the Global Burden of Disease Study 2013显示文摘 | Marie Ng Tom Fleming Margaret Robinson Blake Thomson Nicholas Graetz Christopher Margono Erin C Mullany Stan Biryukov Cristiana Abbafati Semaw Ferede Abera Jerry P Abraham Niveen M E Abu-Rmeileh Tom Achoki Fadia S AlBuhairan Zewdie A Alemu Rafael Alfonso | 2014 | The Lancet2014，，： | 7 |
| 3 | C-reactive protein and radiographic findings of lower respiratory tract infection in infants显示文摘AIM To evaluate the association between C-reactive protein(CRP) and radiological evidence of lower respiratory tract infection(LRTI) in infants.METHODS All patients aged less than 4 years who presented with suspected lower respiratory tract infection,who received a peri-presentation chest radiograph and CRP blood measurement over an 18-mo period were included in the study.Age,gender,source of referral,CRP,white cell count,neutrophil count along with the patients' symptoms and radiologist's report were recorded.RESULTS Three hundred and eleven patients met the inclusioncriteria.Abnormal chest radiographs were more common in patients with elevated CRP levels(P < 0.01).Radiologic signs of LRTI were identified in 73.7% of chest radiographs when a patient had a CRP level between 50-99 mg/L.CRP levels were a better predictor of positive chest radiograph findings for those aged greater than I year compared to those 1 year or less.CONCLUSION CRP may be used in patients with suspected LRTI diagnosis to select those who are likely to have positive findings on chest radiograph,thus reducing unnecessary chest radiographs. | Maria Twomey Hannah Fleming Fiachra Moloney Kevin P Murphy Lee Crush Siobhan B O’Neill Oisin Flanagan Karl James Conor Bogue Owen J O’Connor Michael M Maher | 2017 | World Journal of Radiology2017，9，4： | 2 |
| 4 | Gastric Bypass Leads to Improvement of Diabetic Neuropathy Independent of Glucose Normalization—Results of a Prospective Cohort Study (DiaSurg 1 Study)显示文摘 | Beat P. Müller-Stich Lars Fischer Hannes G. Kenngott Matthias Gondan Jonas Senft Gabriella Clemens Felix Nickel Thomas Fleming Peter P. Nawroth Markus W. Büchler | 2013 | Annals of Surgery2013，，5： | 2 |
| 5 | Behavior of metal alloys in the semi-solid state显示文摘 | M C Flemings | 1991 | Metall Trans1991，22，5： | 2 |
| 6 | Patterns in visual interpretation of coronary after rograms as detected by quantitative coronary arteriography 显示文摘 | Fleming R M Kirkeeide R L Smalling R W | 1991 | J Am Coll Cardiol1991，18，7： | 1 |
| 7 | Behavior of MetalAlloy in the Semi2Sol2id State 显示文摘 | Flemings M C | 1991 | MetalMater TransA1991，，22： | 1 |
| 8 | An overview of evolutionary algorithm in multi-objective optimization显示文摘 | Fonseca C M Fleming P J | 1995 | Evolutionary Computation1995，3，1： | 1 |
| 9 | Behavior of metal alloys in the semi-solid state 显示文摘 | Flemings M C | 1991 | Metallurgical TransactionsA1991，22，5： | 1 |
| 10 | Net-shape forming via semi-solid processing 显示文摘 | Flemings M C | 1993 | Advanced Material and Process1993，，1： | 1 |
| 11 | Immunosurveillance and Survivin-specific T-cell immunity in children with high-risk neuroblastoma显示文摘 | Coughlin C M Fleming M D Carroll R G | 2006 | J Clin Oncol2006，24，36： | 1 |
| 12 | An overview of evolutionary algorithms in multi-objective optimization显示文摘 | Fonseca C M Fleming P J | 1995 | Evolutionary Computation1995，3，1： | 1 |
| 13 | Defects in carbon nanostructures 显示文摘 | ZHOU O FLEMING R M MURPHY D W | 1994 | Science1994，263，5154： | 1 |
| 14 | IGF-I dif- ferentially regulates IGF-binding protein expression in primary mammary fibroblasts and epithelial cells 显示文摘 | Fleming J M Leibowitz B J Kerr D E | 2005 | J Endocrinol2005，186，1： | 1 |
| 15 | Behavior of metal alloys in the semisolid state 显示文摘 | FLEMINGS M C | 1991 | Metallurgical Transactions A1991，22，： | 1 |
| 16 | Treatment of older patients with head and neck cancer: a review 显示文摘 | VanderWalde NA Fleming M Weiss J | 2013 | Onco- logist2013，18，5： | 1 |
| 17 | Fundamental line broadening of single-mode (GaAl) As diode lasers显示文摘 | Fleming M W Mooradian A | 1981 | Applied Physics Letters1981，38，7： | 1 |
| 18 | A rare case of recurrent urachal adenocarcinoma of the bladder显示文摘 | FLEMING M KNOX M KENNEDY MJ | 2013 | Ir Med J2013，106，5： | 1 |
| 19 | Rheocasting显示文摘 | Flemings M C Riekrg Youngkp | 1976 | Materials Science and Engineering1976，25，： | 1 |
| 20 | Ethical dilemmas and human rights considerations arising from the evaluation of a smoking policy in a health promoting setting显示文摘 | HARVEY H D FLEMING P PATTERSON M | 2002 | Int J Environ Health Res2002，12，3： | 1 |
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