|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Human umbilical cord mesenchymal stem cell-loaded amniotic membrane for the repair of radial nerve injury显示文摘In this study,we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits,i.e.,a relatively closed nerve regeneration chamber.After neurolysis,the injured radial nerve was enwrapped with the prepared nerve conduit,which was fixed to the epineurium by sutures,with the cell on the inner surface of the conduit.Simultaneously,a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals.A total of1.75×107cells were seeded on the amniotic membrane.In the control group,patients received only neurolysis.At 12 weeks after cell transplantation,more than 80%of patients exhibited obvious improvements in muscular strength,and touch and pain sensations.In contrast,these improvements were observed only in 55–65%of control patients.At 8 and 12 weeks,muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group.After cell transplantation,no immunological rejections were observed.These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury. | Zhi Li Hanjiao Qin Zishan Feng Wei Liu Ye Zhou Lifeng Yang Wei Zhao Youjun Li | 2013 | Neural Regeneration Research2013，8，36： | 13 |
| 2 | Unexpected complexity of multidrug resistance in the mcr-1-harbouring Escherichia coli显示文摘Dear Editor,The discovery that the mobile colistin resistance gene(mcr-1)is encoded by plasmids and is prevalent in food animals and human beings worldwide(Hasman et al.,2015;Liu et al.,2015)has challenged greatly our traditional idea that polymyxin(consisting of two isoforms:polymyxin B and polymyxin E(colistin))acts as an ultimate line of refuge in the clinical treatment against the severe infections by the multidrug-resistant Gram-negative pathogens(Nation et al.,2015;Paterson and Harris,2015).To make | Rongsui Gao Yue Li Jingxia Lin Chen Tan Youjun Feng | 2016 | Science China(Life Sciences)2016，59，7： | 5 |
| 3 | Construction and genetic analysis of mutator insertion mutant population in maize显示文摘A total of 26718 M1 plants were ob- tained by crossing the active mutator transposon donor parents (Q105, WW51, 115F, V26-2 and 919J) with the recipient parents (Hz85,W328 with Bz gene and S-Mo17Rf3Rf3). The phenotypes of M1 plants were observed in the field. M1 plants were self-pollinated to develop the mutator insertion-mutagenized M2 seeds. The transposition frequency of the mutator in the genome was calculated based on the spotted aleurone phenotype of the M2 seeds. The results showed that: (1) the mutation frequency of M1 phe- notypes in the field was 0.07 in the population of W328×Mu; (2) the mutation frequency of spotted aleurone seeds on the M2 ears was 0.122 in the population of W328×Mu; (3) five S-cytoplasm male-sterile plants were found among 22500 M1 plants of S-Mo17Rf3Rf3×Mu, with the transposition frequency about 2.2×10?4 per locus. 99 flanking se- quences of mutator transposition were amplified by the modified MuTAIL-PCR, and 59 non-redundant sequences with length around 400 bp were obtained. After bioinformatic analysis, 27 sequences of them could be annotated, using non-redundant nucleotide database of maize, rice, and Arabidopsis. 36 se- quences of them were located on the genetic map of maize by comparative genomics, and several flank- ing sequences of mutator insertion were mapped on the single marker locus. Hotspot sequences of mu- tator transposition were revealed by comparing the homologies between the 9-bp target site duplication of the mutator insertion. The putative functions of 8 flanking sequences of mutator transposition had identity with the functions of their correspondingmarker. The constructed mutator insertion mutant population in maize will facilitate the new gene discovery and functional genomics study in maize. | LIU Wenting GAO Youjun TENG Feng SHI Qing ZHENG Yonglian | 2006 | Chinese Science Bulletin2006，51，21： | 4 |
| 4 | Limited distribution and mechanism of the TetX4 tetracycline resistance enzyme显示文摘Tetracyclines,a group of antimicrobial agents targeting protein synthesis,have been extensively used in agricultural,veterinary,and clinic settings[1].The sporadic rise in tetracycline resistance is partially due to the occurrence of efflux pumps and/or ribosome protection[1,2].The growing body of tetracycline-inactivating enzymes(e.g.,TetX(X1 to X4)[3,4]and Tet(47 to 55)[5])represents an alternative mechanism for tetracycline resistance,and threatens the renewed interest of tigecycline as a last-resort defense against lethal infections with carbapenem-resistant Enterobacteriaceae and Acinetobacter species. | Yongchang Xu Lizhang Liu Jian Sun Youjun Feng | 2019 | Science Bulletin2019，64，20： | 4 |
| 5 | Genomic insights into the ESBL and MCR-l-producing ST648 Escherichia coli with multi-drug resistance显示文摘多粘菌素由 multidrug 抵抗的克否定的病原体对严重感染充当避难处的一根最终的线。这个常规想法被活动 colistin 抵抗基因(mcr-1 ) 的最近的发现戏剧性地质问在食物动物和世界范围的人是流行的。更重要地, mcr-1 基因被发现与另外的抗菌素抵抗基因 co 局部性,提起可能性与平底锅药抵抗超级臭虫正在出现。然而,很少在 mcr-1-positive 的染色体上被报导细菌的主人水库。这里,我们报导三个人定序的染色体 mcr-1-positive Escherichia coli (E15004, E15015 和 E15017 ) 孤立并且通过细菌的比较 genomics 的分析定义一般特征。和顺序打字的进一步的 genomic 采矿允许我们阐明 MCR-1-carrying E。coli E15017 属于顺序类型 ST648 和 coproduces 扩大光谱的 -lactamase (ESBL ) 。给 ST648 与任何一个新德里 metallo 为伙伴所知的事实 -- lactamase 1 或 ESBL,我们的结果与 multidrug 电阻作为流行克隆加亮 ST648 的可能性。 | Huimin Zhang Christopher H. Seward Zuowei Wu Huiyan Ye Youjun Feng | 2016 | Science Bulletin2016，61，11： | 4 |
| 6 | Diversified variants of the mcr-1-carrying plasmid reservoir in the swine lung microbiota显示文摘Dear Editor,Emergence of bacteria with multiple drug resistance is a major problem in global public health.Polymyxin comprising polymyxin E(colistin)and polymyxin B is generally recognized as the last-resort antibiotics with | Zhencui Li Chen Tan Jingxia Lin Youjun Feng | 2016 | Science China(Life Sciences)2016，59，9： | 3 |
| 7 | Discovery of multi-drug resistant,MCR-1 and ESBL-coproducing ST117 Escherichia coli from diseased chickens in northeast China显示文摘An endemic multi-drug resistant ST117 E. coli isolate coproducing MCR-1 and 3 ESBL loci was, for the first time, detected from diseased chicken, Liaoning Province, in Northeast China, from 2011 to 2012. Wholegenome sequencing revealed 5 unique plasmids, namely pHXH-1, pHXH-2, pHXH-3, pHXH-4 and pHXH-5). Among them, pHXH1 and pHXH4 encode ESBL, and pHXH-5 mediates MCR-1 colistin resistance. The results indicate that the potentially-national dissemination of MCR-1-positive pathogens with pan-drug resistance proceeds via food chains. | Sijia Ding Xiaohu Han Jun Li Weifan Gao Zeliang Chen Youjun Feng | 2018 | Science Bulletin2018，63，16： | 2 |
| 8 | Study on Design of Interplex Method for GNSS Signal显示文摘 | FENG Youjun WANG Zhong CHEN Dandan | 2014 | China Communications2014，11，A02： | 2 |
| 9 | Direct control of store-operated calcium channels by ultrafast laser显示文摘Ca^(2+) channels are essential to cell birth,life,and death.They can be externally activated by optogenetic tools,but this requires robust introduction of exogenous optogenetic genes for expression of photosensitive proteins in biological systems.Here we present femtoSOC,a method for direct control of Ca^(2+)channels solely by ultrafast laser without the need for optogenetic tools or any other exogenous reagents.Specifically,by focusing and scanning wavelength-tuned low-power femtosecond laser pulses on the plasma membrane for multiphoton excitation,we directly induced Ca^(2+) influx in cultured cells.Mechanistic study reveals that photoexcited flavins covalently bind cysteine residues in Orai1 via thioether bonds,which facilitates Orai1 polymerization to form store-operated calcium channels(SOCs)independently of STIM1,a protein generally participating in SOC formation,enabling all-optical activation of Ca^(2+) influx and downstream signaling pathways.Moreover,we used femtoSOC to demonstrate direct neural activation both in brain slices in vitro and in intact brains of living mice in vivo in a spatiotemporal-specific manner,indicating potential utility of femtoSOC. | Pan Cheng Xiaoying Tian Wanyi Tang Juan Cheng Jin Bao Haipeng Wang Sisi Zheng Youjun Wang Xunbin Wei Tunan Chen Hua Feng Tian Xue Keisuke Goda Hao He | 2021 | Cell Research2021，31，7： | 2 |
| 10 | Streptococcal Toxic Shock Syndrome Caused by Streptococcus suis Serotype 2 显示文摘 | Jiaqi Tang Changjun Wang Youjun Feng | 2006 | PLoS Med2006，3，5： | 1 |
| 11 | Origin, dissemination and entry of the pandemic Zika viruses显示文摘Zika 病毒(ZIKV ) 是打扫了大多数西方的半球的以前忽视,却最新新兴的 zoonotic 病原体。意外注意在新生儿在在 2015 ZIKV 散布到巴西和美国的炸药和它的稀罕关联引起了世界范围的谎言到头小畸型,提起对公共健康的严肃的挑战。我们证明起源和这些流行 ZIKA 的快传播孤立。种系发生的分析表明最新新兴的 ZIKV 孤立的所有属于亚洲美国人的系。ZIKV E 表面蛋白质的结构的分析建议它可能采用类似的入口机制到另外的 Flavi 病毒相似登革热病毒和韦斯特尼罗河病毒的。 | Huimin Zhang Swaminath Srinivas Defeng Li Youjun Feng | 2016 | Science Bulletin2016，61，10： | 1 |
| 12 | Ultra-robust stretchable electrode for e-skin:In situ assembly using a nanofiber scaffold and liquid metal to mimic water-to-net interaction显示文摘The development of stretchable electronics could enhance novel interface structures to solve the stretchability-conductivity dilemma,which remains a major challenge.Herein,we report a nano-liquid metal(LM)-based highly robust stretchable electrode(NHSE)with a self-adaptable interface that mimics water-tonet interaction.Based on the in situ assembly of electrospun elastic nanofiber scaffolds and electrosprayed LM nanoparticles,the NHSE exhibits an extremely low sheet resistance of 52 mΩsq^(-1).It is not only insensitive to a large degree of mechanical stretching(i.e.,350%electrical resistance change upon 570%elongation)but also immune to cyclic deformation(i.e.,5%electrical resistance increases after 330000 stretching cycles with 100%elongation).These key properties are far superior to those of the state-of-the-art reports.Its robustness and stability are verified under diverse circumstances,including long-term exposure to air(420 days),cyclic submersion(30000 times),and resilience against mechanical damages.The combination of conductivity,stretchability,and durability makes the NHSE a promising conductor/electrode solution for flexible/stretchable electronics for applications such as wearable on-body physiological signal detection,human-machine interaction,and heating e-skin. | Jinwei Cao Fei Liang Huayang Li Xin Li Youjun Fan Chao Hu Jing Yu Jin Xu Yiming Yin Fali Li Dan Xu Hanfang Feng Huali Yang Yiwei Liu Xiaodong Chen Guang Zhu Run-Wei Li | 2022 | InfoMat2022，4，4： | 1 |
| 13 | Binding of Shewanella FadR to the fabA fatty acid biosynthetic gene: implications for contraction of the fad regulon显示文摘Escherichia coli fadR 蛋白质产品,一个 paradigm/prototypical FadR 管理者,断然调整 fabA 和 fabB,为不饱和的丰满的酸(UFA ) 的二批评基因生合成。然而,在另外的 proteobacteria 的情形例如有海洋的起源的 Shewanella,是不平常的因为 Rodionov 和共同工作的人预言了那唯一的 fabA (不 fabB ) 为 FadR 有一个有约束力的地点蛋白质。它提起了一度时髦的风尚 regulon 收缩的可能性。这里,我们报导这是案例。FadR 相当或相同的事物的顺序排列表明 N 终端 DNA 有约束力的领域展出了显著类似,而在 C 终点的接受 ligand 主题相对少些被保存。S 的 FadR 相当或相同事物。oneidensis (指了 FadR_she ) 对同质过去表示、净化。FPLC (快蛋白质液体层析) 和化学 cross-linking 分析获得的综合证据阐明了那 FadR_she 蛋白质能在答案使聚合成二聚物,其身份被 MALDI-TOF-MS 决定。在从 electrophoretic 的 vitro 数据,活动性移动试金建议 FadR_she 是到 E 的几乎 functionally-exchangeable/equivalent。处于绑定的能力的 coli FadR (FadR_ec ) E。coli fabA (并且 fabB ) 倡导者。在与 E 的一个协议。coli fabA, S。oneidensis fabA 倡导者绑了 FadR_she 和 FadR_ec,并且与在长链的 acyl-CoA thioesters 的增加之上的 FadR 规章的蛋白质明确地是 disassociated。到在 vivo 的监视器,效果在 Shewanella fabA 表示上由 FadR 施加了, S 的本国的倡导者。oneidensis fabA 被熔化到 LacZ 记者基因在 E 设计染色体 fabA-lacZ transcriptional 熔化。coli。是期望, fadR 基因的移动由女郎活动关于 Shewanella fabA 表示的 2 褶层减少给了,它与由油酸盐的增加的禁止的水平几乎相同。因此,我们断定 fabA 被收缩是在在海洋的细菌 Shewanella 类的丰满的酸合成的上下文的一度时髦的风尚 regulon 的唯一一个成员。 | Huimin Zhang Beiwen Zheng Rongsui Gao Youjun Feng | 2015 | Protein & Cell2015，6，9： | 1 |
| 14 | Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallma 显示文摘 | Kegong Tian Xiuling Yu Tiezhu Zhao Youjun Feng | 2007 | PLOS ONE2007，，6： | 1 |
| 15 | Magnetic nano-beads based separation combined with propidium monoazide treatment and multiplex PCR assay for simultaneous detection of viable Salmonella Typhimurium, Es- cherichia coli 0157:H7 and Listeria monocytogenesin food products显示文摘 | Yang Youjun Xu Feng Xu Hengyi | 2013 | Food Microbiology2013，34，： | 1 |
| 16 | Structure and Cleavage Specificity of the Chymotrypsin-Like Serine Protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)显示文摘 | Xinsheng Tian Guangwen Lu Feng Gao Hao Peng Youjun Feng Guangpeng Ma Mark Bartlam Kegong Tian Jinghua Yan Rolf Hilgenfeld George F. Gao | 2009 | Journal of Molecular Biology2009，，4： | 1 |
| 17 | Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 显示文摘 | JIAQI TANG CHANGJUN WANG YOUJUN FENG | 2006 | Plos Med2006，3，5： | 1 |
| 18 | Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 2 显示文摘 | Jiaqi Tang Changjun Wang Youjun Feng | 2006 | PLoS Med2006，3，5： | 1 |
| 19 | Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core显示文摘 | Guangpeng Ma Youjun Feng Feng Gao Jinzi Wang Cheng Liu Yijing Li | 2005 | Biochemical and Biophysical Research Communications2005，，4： | 1 |
| 20 | The involvement of sortase A in high virulence of STSS-causing Streptococcus suis serotype 2显示文摘 | Changjun Wang Ming Li Youjun Feng Feng Zheng Yaqing Dong Xiuzhen Pan Gong Cheng Ruiping Dong Dan Hu Xiaodan Feng Junchao Ge Di Liu Jing Wang Min Cao Fuquan Hu Jiaqi Tang | 2008 | Archives of Microbiology2008，，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员