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| 1 | Molecular determinants of the antitumor effects of trichostatin A in pancreatic cancer cells显示文摘AIM:To gain molecular insights into the action of the histone deacetylase inhibitor(HDACI) trichostatin-A(TSA) in pancreatic cancer(PC) cells.METHODS:Three PC cell lines,BxPC-3,AsPC-1 and CAPAN-1,were treated with various concentrations of TSA for def ined periods of time.DNA synthesis was assessed by measuring the incorporation of 5-bromo-2'deoxyuridine.Gene expression at the level of mRNA was quantif ied by real-time polymerase chain reaction.Expression and phosphorylation of proteins was monitored by immunoblotting,applying an infrared imaging technology.To study the role of p38 MAP kinase,the specif ic enzyme inhibitor SB202190 and an inactive control substance,SB202474,were employed.RESULTS:TSA most eff iciently inhibited BrdU incorporation in BxPC-3 cells,while CAPAN-1 cells displayed the lowest and AsPC-1 cells an intermediate sensitivity.The biological response of the cell lines correlated with the increase of histone H3 acetylation after TSA application.In BxPC-3 cells(which are wild-type for KRAS),TSA strongly inhibited phosphorylation of ERK 1/2 and AKT.In contrast,activities of ERK and AKT in AsPC-1 and CAPAN-1 cells(both expressing oncogenic KRAS) were not or were only modestly affected by TSA treatment.In all three cell lines,but most pronounced in BxPC-3 cells,TSA exposure induced an activation of the MAP kinase p38.Inhibition of p38 by SB202190 slightly but signif icantly diminished the antiproliferative effect of TSA in BxPC-3 cells.Interestingly,only BxPC-3 cells responded to TSA treatment by a signif icant increase of the mRNA levels of bax,a pro-apoptotic member of the BCL gene family.Finally,in BxPC-3 and AsPC-1 cells,but not in the cell line CAPAN-1,signif icantly higher levels of the cell cycle inhibitor protein p21Waf1 were observed after TSA application.CONCLUSION:The biological effect of TSA in PC cells correlates with the increase of acetyl-H3,p21Waf1,phospho-p38 and bax levels,and the decrease of phosphoERK 1/2 and phospho-AKT. | Elisabeth Emonds Brit Fitzner Robert Jaster | 2010 | World Journal of Gastroenterology2010，16，16： | 5 |
| 2 | Prediction of delayed graft function using different scoring algorithms: A single-center experience显示文摘AIM To compare the performance of 3 published delayed graftfunction(DGF) calculators that compute the theoretical risk of DGF for each patient.METHODS This single-center,retrospective study included 247 consecutive kidney transplants from a deceased donor.These kidney transplantations were performed at our institution between January 2003 and December 2012.We compared the occurrence of observed DGF in our cohort with the predicted DGF according to three different published calculators. The accuracy of the calculators was evaluated by means of the c-index(receiver operating characteristic curve).RESULTS DGF occurred in 15.3% of the transplants under study.The c index of the Irish calculator provided an area under the curve(AUC) of 0.69 indicating an acceptable level of prediction,in contrast to the poor performance of the Jeldres nomogram(AUC = 0.54) and the Chapal nomogram(AUC = 0.51). With the Irish algorithm the predicted DGF risk and the observed DGF probabilities were close. The mean calculated DGF risk was significantly different between DGF-positive and DGF-negative subjects(P < 0.0001). However,at the level of the individual patient the calculated risk of DGF overlapped very widely with ranges from 10% to 51% for recipients with DGF and from 4% to 56% for those without DGF.The sensitivity,specificity and positive predictive value of a calculated DGF risk ≥ 30% with the Irish nomogram were 32%,91% and 38%. CONCLUSION Predictive models for DGF after kidney transplantation are performant in the population in which they were derived,but less so in external validations. | Magda Michalak Kristien Wouters Erik Fransen Rachel Hellemans Amaryllis H Van Craenenbroeck Marie M Couttenye Bart Bracke Dirk K Ysebaert Vera Hartman Kathleen De Greef Thiery Chapelle Geert Roeyen Gerda Van Beeumen Marie-Paule Emonds Daniel Abramowicz Jean-Louis Bosmans | 2017 | World Journal of Transplantation2017，7，5： | 3 |
| 3 | Hypothermic Machine Preservation Reduces Molecular Markers of Ischemia/Reperfusion Injury in Human Liver Transplantation显示文摘 | S. D. Henry E. Nachber J. Tulipan J. Stone C. Bae L. Reznik T. Kato B. Samstein J. C. Emond J. V. Guarrera | 2012 | American Journal of Transplantation2012，，9： | 2 |
| 4 | Model for end-stage liver disease and Child-Turcotte-Pugh score as predictors of pretransplantation disease severity, posttransplantation outcome, and resource utilization in United Network for Organ Sharing status 2A patients显示文摘 | Robert S. Brown K.Shiva Kumar Mark W. Russo Milan Kinkhabwala Dianne L. Rudow Patricia Harren Steven Lobritto Jean C. Emond | 2002 | Liver Transplantation2002，，3： | 2 |
| 5 | Prevention of De Novo Hepatitis B in Recipients of Core Antibody-Positive Livers With Lamivudine and Other Nucleos(t)ides: A 12-Year Experience显示文摘 | Matthew S. Chang Sonja K. Olsen Elsa M. Pichardo Jessica B. Stiles Lori Rosenthal-Cogan William D. Brubaker James V. Guarrera Jean C. Emond Robert S. Brown | 2013 | Transplantation Journal2013，，7： | 2 |
| 6 | Progressive necrosis after hepatectomy and the pathophysiology of liver failure after massive resection显示文摘 | Yves Panis David M McMullan Jean C Emond | 1997 | Surgery1997，，2： | 2 |
| 7 | Efficacy of Neoadjuvant Chemoradiation, Followed by Liver Transplantation, for Perihilar Cholangiocarcinoma at 12 US Centers显示文摘 | Sarwa Darwish Murad W. Ray Kim Denise M. Harnois David D. Douglas James Burton Laura M. Kulik Jean F. Botha Joshua D. Mezrich William C. Chapman Jason J. Schwartz Johnny C. Hong Jean C. Emond Hoonbae Jeon Charles B. Rosen Gregory J. Gores Julie K. Heimbac | 2012 | Gastroenterology2012，，1： | 2 |
| 8 | Negative Impact of Neutrophil-Lymphocyte Ratio on Outcome After Liver Transplantation for Hepatocellular Carcinoma显示文摘 | Karim J. Halazun Mark A. Hardy Abbas A. Rana David C. Woodland Elijah J. Luyten Suhari Mahadev Piotr Witkowski Abbey B. Siegel Robert S. Brown Jean C. Emond | 2009 | Annals of Surgery2009，，1： | 2 |
| 9 | Hepatitis C,iron status,and disease severity:relationship with HFE mutations显示文摘 | TUNG B Y EMOND M J BRONNER M P | 2003 | Gastroenterology2003，124，2： | 1 |
| 10 | Chest computed tomogra-phy findings in bronchopulmonary dysplasia and correlationwith lung function 显示文摘 | Mahut B De Blic J Emond S | 2007 | Arch Dis Child Fetal Neonatal Ed2007，92，6： | 1 |
| 11 | Mierostruetured Fiber Splieing显示文摘 | Bourliaguet B Pare C Emond F Croteau A Proulx A Vallee R | 2003 | Optics Express2003，11，25： | 1 |
| 12 | Application of reduced size liver transplants as split grafts, auxiliary orthotopic grafts, and living related segmental transplants显示文摘 | Broelsch CE Emond JC Whitington PF | 1990 | Ann Surg1990，212，3： | 1 |
| 13 | Substituted Diphenyl Sulfides asSelective Serotonin Transporter Ligands: Synthesis and in Vitro Evaluation显示文摘 | Emond P Vercouillie J Innis R | 2002 | Med Chem2002，45，： | 1 |
| 14 | Molecular characterizaion of a theta replication plasmid and its use for de-velopment of a two-component food-grade cloning system for Lactococcus lactis显示文摘 | Emond E Lavailee R Drolet G | 2001 | Applied and Environmental Mi-crobiology2001，67，： | 1 |
| 15 | Energy Drink Consumption and the Risk of Alcohol Use Disorder among a National Sample of Adolescents and Young Adults显示文摘 | Jennifer A. Emond Diane Gilbert-Diamond Susanne E. Tanski James D. Sargent | 2014 | The Journal of Pediatrics2014，，： | 1 |
| 16 | Prostaglandin E2 regulates both interleukin-2 and granulocyte-macrophage colonystimulating factor gene expression in bovine lymphocytes 显示文摘 | Emond V Fortier M A Murphy B D | 1998 | Biol Reprod1998，58，1： | 1 |
| 17 | Microstructurod fiber splicing显示文摘 | BOURLIAGUETB PARE C EMOND F | 2003 | Optics Express2003，11，25： | 1 |
| 18 | AIDA, a class Ⅰ metabotropic glutamate-receptor antagonist limits kainite-induced hippocampal dysfunction 显示文摘 | Renaud J Emond M Meillcur S | 2002 | Epilepsia2002，43，11： | 1 |
| 19 | Analysis of the National Institute of Neurological Disorders and Stroke tissue plasminogen activator studies following European Cooperative Acute Stroke Study Ⅲ patient selection criteria显示文摘 | Hemmen T M Rapp K S Emond J A | 2010 | J Stroke Cerebrovasc Dis2010，19，4： | 1 |
| 20 | Molecular Characterization of a Theta Replication Plasmid and its Use for Development of a Two-component Food-grade Cloning System for Lactococcus lactis显示文摘 | EMOND E LAVALL(E)E R DROLET G | 2001 | Applied and Environmental Microbiology2001，67，4： | 1 |
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