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| 1 | Herbal decoctosome is a novel form of medicine显示文摘Traditionally, herbal medicine is consumed by drinking decoctions produced by boiling herbs with water. The functional components of the decoction are heat stable. Small RNAs(sRNAs) were reported as a new class of functional components in decoctions. However, the mechanisms by which sRNAs survive heat treatment of the decoction and enter cells are unclear.Previous studies showed that plant-derived exosome-like nanoparticles(ELNs), which we call botanosomes, could deliver therapeutic reagents in vivo. Here, we report that heat-stable decoctosomes(ELNs) from decoctions have more therapeutic effects than the decoctions in vitro and demonstrate therapeutic efficacy in vivo. Furthermore, sRNAs, such as HJT-sRNA-m7 and PGY-sRNA-6, in the decoctosome exhibit potent anti-fibrosis and anti-inflammatory effects, respectively. Decoctosome is comprised of lipids, chemical compounds, proteins, and s RNAs. A medical decoctosome mimic is called bencaosome. A single lipid sphinganine(d22:0) identified in the decoctosome was mixed and heated with the synthesized sRNAs to form the simplest bencaosome. This simple bencaosome structure was identified by critical micelle concentration(cmc) assay that sRNAs coassembled with sphinganine(d22:0) to form the lipid layers of vesicles. The heating process facilitates co-assembly of sRNAs and sphinganine(d22:0) until a steady state is reached. The artificially produced sphinganine-HJT-sRNA-m7 and sphinganinePGY-sRNA-6 bencaosomes could ameliorate bleomycin-induced lung fibrosis and poly(I:C)-induced lung inflammation, respectively, following oral administration in mice. Our study not only demonstrates that the herbal decoctosome may represent a combinatory remedy in precision medicine but also provides an effective oral delivery route for nucleic acid therapy. | Xiaoyun Li Zhu Liang Jianchao Du Zhiqing Wang Song Mei Zhiqing Li Yan Zhao DANDan Zhao Yiming Ma Jun Ye Jiantao Xu Yu Zhao Jiahui Chang Yuhao Qin Lanlan Yu Chenxuan Wang Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 26 |
| 2 | Plant-derived phosphocholine facilitates cellular uptake of anti-pulmonary fibrotic HJT-sRNA-m7显示文摘Pulmonary fibrosis, a progressive chronic disease with a high mortality rate, has limited treatment options. Currently, lung transplantation remains the only effective treatment. Here we report that a small RNA, HJT-sRNA-m7, from a Chinese herbal medicine Hong Jing Tian(HJT, RHODIOHAE CRENULATAE RADIX ET RHIZOMA, Rhodiola crenulata) can effectively reduce the expressions of fibrotic hallmark genes and proteins both in alveolar in vitro and in mouse lung tissues in vivo. We also discovered over one hundred oil-soluble chemicals from HJT decoctions, most of which are found in lipid extracts from other Chinese herbals decoctions, including Pu Gong Ying(PGY, TARAXACI HERBA, Taraxacum mongolicum), Chuan Xin Lian(CXL, changed to 'ANDROGRAPHIS HERBA, Andrographis paniculata'), and Jin Yin Hua(JYH, lonicera japonica or Honeysuckle). We identified the active component in these decoctions as two forms of phosphocholines, PC(18:0/18:2) and PC(16:0/18:2). These PCs potentially could form liposomes with small RNAs to enter human alveolar and gastric cells. Our experimental results suggest an unprecendent lipid complex route through which botanic small RNA can enter human bodies.Our results provide an innovative treatment strategy for oral delivery of siRNAs as therapeutic medication. | Jianchao Du Zhu Liang Jiantao Xu Yan Zhao Xiaoyun Li Yanli Zhang DANDan Zhao Ruxuan Chen Yang Liu Trupti Joshi Jiahui Chang Zhiqing Wang Yanxu Zhang Jindong Zhu Qiang Liu Dong Xu Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 21 |
| 3 | Large-scale analysis of small RNAs derived from traditional Chinese herbs in human tissues显示文摘Plant-derived microRNAs have recently been reported to function in human blood and tissues. Controversy was immediately raised due to possible contamination and the lack of large sample sizes. Here, we report thousands of unique small RNAs derived from traditional Chinese medicine(TCM) herbs found in human blood cells and mouse lung tissues using a large-scale analysis.We extracted small RNAs from decoctions of 10 TCM plants(Ban Zhi Lian, Chai Hu, Chuan Xin Lian, Di Ding Zi Jin, Huang Qin, Jin Yin Hua, Lian Qiao, Pu Gong Ying, Xia Ku Cao, and Yu Xing Cao) and obtained millions of RNA sequences from each herb. We also obtained RNA-Seq data from the blood cells of humans who consumed herbal decoctions and from the lung tissues of mice administered RNAs from herbal decoctions via oral gavage. We identified thousands of unique small RNA sequences in human blood cells and mouse lung tissues. Some of these identified small RNAs from Chuan Xin Lian and Hong Jing Tian could be mapped to the genomes of the herbs, confirming their TCM plant origin. Small RNAs derived from herbs regulate mammalian gene expression in a sequence-specific manner, and thus are a superior novel class of herbal drug components that hold great potential as oral gene-targeted therapeutics, highlighting the important role of herbgenomics in their development. | Fengming Huang Jianchao Du Zhu Liang Zhichao Xu Jiantao Xu Yan Zhao Yexuan Lin Song Mei Quan He Jindong Zhu Qiang Liu Yanxu Zhang Yuhao Qin Wei Sun Jingyuan Song Shilin Chen Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 14 |
| 4 | Angiotensin II receptor blocker as a novel therapy in acute lung injury induced by avian influenza A H5N1 virus infection in mouse显示文摘Dear Editor,Since the first recognized case of human infection with avian influenza H5N1 virus in 1997,the worldwide spread and re-emergence of this highly pathogenic influenza virus have led to 694 human infection cases.Of these cases,402died from 2003 to 2015(http://gffzz36b398b81f8a4a4ch0qkkq05fccup6vwx.ffgz.tsg.suse.edu.cn/influenza/humananimalinterface/ENGIP20150106CumulativeNumber H5N1cases.pdf?ua=1),with a morbidity rate | YAN YiWu LIU Qiang LI Ning DU JianChao LI Xiao LI Chang JIN NingYi JIANG ChengYu | 2015 | Science China(Life Sciences)2015，58，2： | 12 |
| 5 | Hsa-miR-1246, hsa-miR-320a and hsa-miR-196b-5p inhibitors can reduce the cytotoxicity of Ebola virus glycoprotein in vitro显示文摘Ebola virus(EBOV)causes a highly lethal hemorrhagic fever syndrome in humans and has been associated with mortality rates of up to 91%in Zaire,the most lethal strain.Though the viral envelope glycoprotein(GP)mediates widespread inflammation and cellular damage,these changes have mainly focused on alterations at the protein level,the role of microRNAs(miRNAs)in the molecular pathogenesis underlying this lethal disease is not fully understood.Here,we report that the miRNAs hsa-miR-1246,hsa-miR-320a and hsa-miR-196b-5p were induced in human umbilical vein endothelial cells(HUVECs)following expression of EBOV GP.Among the proteins encoded by predicted targets of these miRNAs,the adhesion-related molecules tissue factor pathway inhibitor(TFPI),dystroglycan1(DAG1)and the caspase 8 and FADD-like apoptosis regulator(CFLAR)were significantly downregulated in EBOV GP-expressing HUVECs.Moreover,inhibition of hsa-miR-1246,hsa-miR-320a and hsa-miR-196b-5p,or overexpression of TFPI,DAG1 and CFLAR rescued the cell viability that was induced by EBOV GP.Our results provide a novel molecular basis for EBOV pathogenesis and may contribute to the development of strategies to protect against future EBOV pandemics. | SHENG MiaoMiao ZHONG Ying CHEN Yang DU JianChao JU XiangWu ZHAO Chen ZHANG GuiGen ZHANG LiFang LIU KangTai YANG Ning XIE Peng LI DangSheng ZHANG Michael Q. JIANG ChengYu | 2014 | Science China(Life Sciences)2014，57，10： | 7 |
| 6 | Hundred-Joule-level,nanosecond-pulse Nd:glass laser system with high spatiotemporal beam quality显示文摘A 100-J-level Nd:glass laser system in nanosecond-scale pulse width has been constructed to perform as a standard source of high-fluence-laser science experiments. The laser system, operating with typical pulse durations of 3–5 ns and beam diameter 60 mm, employs a sequence of successive rod amplifiers to achieve 100-J-level energy at 1053 nm at3 ns. The frequency conversion can provide energy of 50-J level at 351 nm. In addition to the high stability of the energy output, the most valuable of the laser system is the high spatiotemporal beam quality of the output, which contains the uniform square pulse waveform, the uniform flat-top spatial fluence distribution and the uniform flat-top wavefront. | Sensen Li Yulei Wang Zhiwei Lu Lei Ding Yi Chen Pengyuan Du Dexin Ba Zhenxing Zheng Xin Wang Hang Yuan Chengyu Zhu Weiming He Dianyang Lin Yongkang Dong Dengwang Zhou Zhenxu Bai Zhaohong Liu Can Cui | 2016 | High Power Laser Science and Engineering2016，4，1： | 3 |
| 7 | A comprehensive analysis of the Bencao(herbal)small RNA Atlas reveals novel RNA therapeutics for treating human diseases显示文摘Cross-kingdom herbal mi RNA was first reported in 2012.Using a modified herbal extraction protocol,we obtained 73,677,287sequences by RNA-seq from 245 traditional Chinese Medicine(TCM),of which 20,758,257 were unique sequences.We constructed a Bencao(herbal)small RNA(s RNA)Atlas(http://gffzz4a3adbfa122d4a47h0qkkq05fccup6vwx.ffgz.tsg.suse.edu.cn),annotated the sequences by sequence-based clustering,and created a nomenclature system for Bencao s RNAs.The profiles of 21,757 mi RNAs in the Atlas were highly consistent with those of plant mi RNAs in mi RBase.Using software tools,our results demonstrated that all human genes might be regulated by s RNAs from the Bencao s RNA Atlas,part of the predicted human target genes were experimentally validated,suggesting that Bencao s RNAs might be one of the main bioactive components of herbal medicines.We established roadmaps for oligonucleotide drugs development and optimization of TCM prescriptions.Moreover,the decoctosome,a lipo-nano particle consisting of 0.5%–2.5%of the decoction,demonstrated potent medical effects.We propose a Bencao(herbal)Index,including small-molecule compounds(SM),protein peptides(P),nucleic acid(N),non-nucleic and non-proteinogenic large-molecule compounds(LM)and elements from Mendeleev's periodic table(E),to quantitatively measure the medical effects of botanic medicine.The Bencao s RNA Atlas is a resource for developing gene-targeting oligonucleotide drugs and optimizing botanical medicine,and may provide potential remedies for the theory and practice of one medicine. | Yinghao Cao Yexuan Lin Na Sun Xinyi Du Yixin Dong Song Mei Xingyu Deng Xiaobei Li Shaoting Guo Kegong Tang Jiaqi Liu Xiangyu Qiao Dandan Zhao Yuhao Qin Cong Zhang Tianyi Xin Xiaohu Shi Congzhao Zhou Tao Dong De-an Guo Benedikt MKessler Dong Xu Jingyuan Song Fengming Huang Xiaoyue Wang Chengyu Jiang | 2023 | Science China(Life Sciences)2023，66，10： | 2 |
| 8 | Erratum to:Herbal decoctosome is a novel form of medicine显示文摘Following the published article,we noticed an error duplication in Figure 5G“control”and“PGY-6”that was introduced during the revised process,with an attempt to replace it with higher-resolution images.Here we provide the original data in the first submitted manuscript(Figure 5G). | Xiaoyun Li Zhu Liang Jianchao Du Zhiqing Wang Song Mei Zhiqing Li Yan Zhao Dandan Zhao Yiming Ma Jun Ye Jiantao Xu Yu Zhao Jiahui Chang Yuhao Qin Lanlan Yu Chenxuan Wang Chengyu Jiang | 2020 | Science China(Life Sciences)2020，63，9： | 2 |
| 9 | Nonlinear short-term heart rate variability prediction of spontaneous ventricular tachyarrhythmia显示文摘As malign ventricular tachyarrhythmias triggering sudden cardiac death (SCD), both ventricular tachycardia (VT) and ventricular fibrillation (VF) are major causes of mortality. The most efficient ther- apy for SCD prevention is implantable cardioverter defibrillators (ICD). The ICD can accurately and ef- fectively identify the forthcoming of fatal ventricular tachyarrhythmias and deliver a shock in order to restore patients’ normal sinus rhythm. In this study, two nonlinear complexity measures based on en- tropy: approximate entropy (ApEn) and sample entropy (SampEn) as well as two time linear indices: the mean RR interval (the average of time intervals between consecutive R-waves) and the standard devia- tion of RR intervals were used for short-term forecasting of VT-VF occurrence. The last small sections of interbeat intervals preceding 135 VT-VF episodes from 78 patients stored by the ICD were analyzed and compared with individually acquired control time series (CON series) from the same patients, which are normally intrinsic sinus rhythms. The results demonstrate that in addition to an obvious in- crease in heart rates of the patients, the values of two entropy measures are significantly smaller for VT-VF episodes than those for CON series. Conclusions can be drawn that when a ventricular tach- yarrhythmia approaches, the sympathetic tone of the patients is increased, and the complexity of their RR intervals immediately before the onset of VT-VF events is obviously lower than that of RR intervals recorded during sinus rhythms. For a better separation, the optimal range of threshold r is determined for two algorithms. ApEn and SampEn measures might be the suitable nonlinear parameters for short- term prediction of life-threatening ventricular tachyarrhythmias in the application of the cardioversion and defibrillation. | ZHUANG JianJun NING XinBao DU SiDan WANG ZhenZhou HUO ChengYu YANG Xi FAN AiHua | 2008 | Chinese Science Bulletin2008，53，16： | 2 |
| 10 | The Uniform Distribution of SiC Particles in an A356-SiC_p Composite Produced by the Tilt-blade Mechanical Stirring显示文摘The tilt-blade mechanical stirring of A356-2.5vol.%SiCp liquid was conducted in a cylindrical crucible to solve the problem of nonhomogeneous radial distribution of SiC particles in conventional straight-blade mechanical stirring. The radial distribution of SiC particles in A356 liquid was studied under the conditions of 25° for horizontal tilt angle α of the blade, 200 r/min for rotating speed of stirrer and 10 mm/s for speed of moving up and down of stirrer. The results show that there exists a nonlinear relationship between circumferential tilt angle β of the blade and radial relative deviation d of SiCp content in A356 liquid. When β is smaller than 26°, the d of SiCp content in A356 liquid between the center and the periphery of crucible decreases with increasing β. Conversely, when β is bigger than 26 , d increases with increasing β. Only when β is about 26°, d can be equal to nought i.e. uniform radial distribution of SiC particles can be realized. It can be seen that the nonhomogeneous radial distribution of SiC particles in conventional straight-blade mechanical stirring can be eliminated in this tilt-blade mechanical stirring of A356-SiCp liquid. | Yunhui DU Peng ZHANG Yujie WANG Jun ZHANG Shasha YAO Chengyu LI | 2013 | Acta Metallurgica Sinica(English Letters)2013，26，1： | 2 |
| 11 | Effects of chitooligo- saccharides on rabbit neutrophi|s in vitro 显示文摘 | Dou Jiangli Tan Chengyu Du Yuguang | 2007 | Carbohydr Polym2007，69，2： | 1 |
| 12 | Use of oxidoreduction potential as an indicator to regulate 1,3-propanediol fermentation by Klebsiella pneumoniae 显示文摘 | Du Chengyu Yan Hui Zhang Yanping | 2006 | Appl Microbiol Biotechnol2006，69，5： | 1 |
| 13 | Oral administration of the herbal oligonucleotide XKC-sRNA-h3 prevents angiotensinⅡ-induced hypertension in mice显示文摘Hypertension has become a growing public health concern worldwide.In fact,hypertension is commonly associated with increased morbidity and mortality.Currently,oligonucleotide drugs have proven to be promising therapeutic agents for various diseases.In the present study,we aimed to demonstrate that a herbal small RNA(s RNA),XKC-sRNA-h3(B55710460,F221.I000082.B11),exhibits potent antihypertensive effects by targeting angiotensin-converting enzyme(ACE)in mice.When compared with captopril,oral administration of the sphingosine(d18:1)-XKC-s RNA-h3 bencaosome more effectively prevented angiotensin II-induced hypertensive cardiac damage and alleviated kidney injury in mice.Such findings indicated that XKC-sRNA-h3 may be a novel orally available ACE inhibitor type oligonucleotide drug for hypertension. | Kegong Tang Xiaona Wang Yu Zhao Xiaobei Li Zhenyu Jiang Song Mei Mingrui Chen Yiming Ma Xinyi Du Xiangyu Qiao Na Sun Jiaqi Liu Chengyu Jiang | 2023 | Science China(Life Sciences)2023，66，10： | 1 |
| 14 | Effects of chitooligosaccharides on rabbit neutrophils in vitro 显示文摘 | Dou Jiangli Tan Chengyu Du Yuguang | 2007 | Carbohydr Polym2007，69，： | 1 |
| 15 | A modified Sutherland equation for obtaining diffusivities in liquid and its effect on microstructure simulation of WC-Ti(C,N)-Co gradient cement-ed carbides显示文摘 | Chen Weimin Zhang Lijun Du Yong Liu Dandan Zhang Weibin Cui Senlin Tan Chengyu Wang Shequan Wen Guanghua Xie Wen | 2012 | Metall Mater Trans A2012，，： | 1 |
| 16 | Spatial beam shaping for high-power frequency tripling lasers based on a liquid crystal spatial light modulator显示文摘 | Sensen Li Yulei Wang Zhiwei Lu Lei Ding Can Cui Yi Chen Du Pengyuan Dexin Ba Zhenxing Zheng Hang Yuan Lei Shi Zhenxu Bai Zhaohong Liu Chengyu Zhu Yongkang Dong Luoxian Zhou | 2016 | Optics Communications2016，，： | 1 |
| 17 | Milk Consumption and Lactose Intolerance in Adults显示文摘Objective To investigate relations between milk consumption and lactose intolerance (LI) in adults and to explore the effect of milk consumption on lactase activity.Methods Total of 182 subjects aged 20‐70 years were recruited and interviewed by questionnaires,and their accumulative cow's milk intake (AMI) was calculated.LI was evaluated by hydrogen breath test (HBT).Results A negative correlation was found between AMI and severity of observed LI symptom (r=-0.2884;P<0.05).Binary logistic regression analysis showed a negative correlation between LI and duration and frequency of milk consumption (OR,0.317 and 0.465,respectively;both P<0.05) and a positive correlation between LI and amount of milk consumed per sitting (OR,6.337;P<0.05).Conclusion LI is related to various milk consumption behaviors.Most Chinese adults with LI may tolerate moderate milk consumption <160 mL. | QIAO Rong HUANG ChengYu DU HuiZhang ZENG Guo LI Ling YE Sheng | 2011 | Biomedical and Environmental Sciences2011，24，5： | 1 |
| 18 | Effects of ehitooligosac-charides on rabbit neutrophils in vitro显示文摘 | DOU Jiangli TAN Chengyu DU Yuguang | 2007 | Carbohydr Polym2007，69，： | 1 |
| 19 | Effects of chitooligosaccharides on rabbit neutrophils in vitro 显示文摘 | Jiangli Dou Chengyu Tan Yugu ang Du | 2007 | Carbohydrate Polymers2007，69，2： | 1 |
| 20 | Potential oligonucleotide drugs in Bencao sRNA Atlas显示文摘Dear Editor,We read the Letter of Professor Shanfa Lu(Lu,2023)titled“Are herbal sRNAs really novel precision medicines?”In this Letter,Lu(2023)commented on our previously published manuscripts(Du et al.,2019;Huang et al.,2019)and concluded that“HJT-sRNA-m1–HJT-sRNA-m8,the eight sRNAs thought to be derived from R.crenulata,are actually derived from animal and/or human beings”. | Fengming Huang Jiaqi Liu Sifen Du Yexuan Lin Chengyu Jiang | 2023 | Science China(Life Sciences)2023，66，12： | 0 |
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