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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Causes of encephalitis and differences in their clinical presentations in England: a multicentre, population-based prospective study显示文摘 | Julia Granerod Helen E Ambrose Nicholas WS Davies Jonathan P Clewley Amanda L Walsh Dilys Morgan Richard Cunningham Mark Zuckerman Ken J Mutton Tom Solomon Katherine N Ward Michael PT Lunn Sarosh R Irani Angela Vincent David WG Brown Natasha S Crowcroft | 2010 | The Lancet Infectious Diseases2010，，12： | 2 |
| 3 | Type I error rates from mixed effects model repeated measures versus fixed effects ANOVAwith missing values imputed via Last Observation Carried Forward 显示文摘 | Mallinckredt CH Clark WS David SR | 2001 | Drug Information J2001，35，4： | 1 |
| 4 | Diufinal blood pressure variation in the evaluation of early onset severe pre-eclampsia显示文摘 | Daniel WS Hein JO David RH | 2008 | Eur J Obstet Gynecol Reprod Biology2008，138，： | 1 |
| 5 | Improving electrophysio- logic and histologic outcomes by photochemically sealing amnion to the peripheral nerve repair site显示文摘 | Henry FP Goyal NA David WS | 2009 | Surgery2009，145，3： | 1 |
| 6 | Coexistence of sleep apnea and asthma re- suiting in severe sleep hypoxemia 显示文摘 | David WH David WS | 1979 | JAMA1979，242，25： | 1 |
| 7 | Pulmonary embolus after vena cava filter placement 显示文摘 | David W Gross WS Colaiuta E | 1999 | Am Surg1999，65，4： | 1 |
| 8 | Normal flow ( TIMI-3 ) before mechanical reperfusion therapy is an independent determinant of survival in acute myocardial infarction: analysis from the primary angioplasty in myocardial infarction trials 显示文摘 | Gregg WS David C Eulogio G | 2001 | Circulation2001，104，： | 1 |
| 9 | Human cholecystokinin-A receptor is not an oncofetal protein 显示文摘 | David WS Msc MD Chi SO | 2000 | Dig Dis Sci2000，45，3： | 1 |
| 10 | Pulmonary embolus after vena cava filter placement显示文摘 | David W Gross WS Colaiuta E | 1999 | Am surg1999，65，4： | 1 |
| 11 | Diurinal blood pressure variation in the evaluation of early onset severe pre-eclampsia显示文摘 | Daniel WS Hein JO David RH | 2008 | Eur Jobstet Gynecol Reprod Biology2008，138，： | 1 |
| 12 | Myoglobinuria显示文摘 | David WS | 2000 | Neurol Clin2000，18，1： | 1 |
| 13 | Myoglobinuria显示文摘 | David WS | 2000 | Neurol Clin2000，18，1： | 1 |
| 14 | Pulmonary embolus after vena cava filter placement显示文摘 | David W Gross WS Colaiuta E | 1999 | Am Surg1999，65，4： | 1 |
| 15 | Adjustable shunt valve programmable at home : safety and feasibility 显示文摘 | Christian WS David S David ME | 2007 | Neurosurgery2007，60，： | 1 |
| 16 | Myoglobinuria显示文摘 | DAVID WS | | 0，，01： | 1 |
| 17 | Puhnonary embolus after vena cava filter placement 显示文摘 | David W Gross WS Colaiuta E | 1999 | Am Surg1999，65，4： | 1 |
| 18 | Specialised treatment based on cognitive behaviour therapy versus usual care for tinnitus: a randomised controlled trial显示文摘 | Rilana FF Cima Iris H Maes Manuela A Joore Dyon JWM Scheyen Amr El Refaie David M Baguley Lucien JC Anteunis Gerard JP van Breukelen Johan WS Vlaeyen | 2012 | The Lancet2012，，9830： | 1 |
| 19 | Reduced constrictor reactivity balances impaired vasodilation in the mesenteric circulation of the obese Zucker rat显示文摘 | Olga PR David WS | 2005 | Am J Physiol Heart Circ Physiol2005，289，5： | 1 |
| 20 | Pulmonary embolus after vena cava filter placement 显示文摘 | David W Gross WS Colaiuta E | 1999 | Am Surg1999，65，4： | 1 |
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