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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | 胎盘生长因子抑制胎盘缺血引起的高血压显示文摘先兆子痫最有效的治疗是提前分娩出胎儿和胎盘。胎盘缺血的发生对先兆子痫发病有至关重要的影响,因为在动物或人类,子宫灌注压减少(reduced uterine perfusion pressure,RUPP)可诱发胎盘缺血/缺氧,刺激机体释放高血压胎盘因子进入母体循环。抗血管生成因子可溶性fms样酪氨酸激酶1(soluble fms-like tyrosine kinase-1,sFlt-1)可拮抗和减少有生物活性的血管内皮生长因子( vascular endothelial growth factor, VEGF)和胎盘生长因子( placental growth factor, PIGF),研究发现在RUPP大鼠和先兆子痫患者中,sFlt-1水平升高。 | 刘莉 叶鹏 Spradley FT Tan AY Joo WS Daniels G Kussie P Karumanchi SA Granger JP | 2016 | 中华高血压杂志2016，24，2： | 2 |
| 3 | Diufinal blood pressure variation in the evaluation of early onset severe pre-eclampsia显示文摘 | Daniel WS Hein JO David RH | 2008 | Eur J Obstet Gynecol Reprod Biology2008，138，： | 1 |
| 4 | Homocysteine-associated acute mesenteric artery occlusion treated with thrombectomy and bowel resection显示文摘 | Gradamn WS Daniel J Miller B | 2001 | Ann Vasc Surg2001，15，2： | 1 |
| 5 | Imaging of soft tissue infectiom显示文摘 | Daniel WS Peter LM Mark JL | 2001 | Radio Clin Nor Am2001，39，2： | 1 |
| 6 | Diurinal blood pressure variation in the evaluation of early onset severe pre-eclampsia显示文摘 | Daniel WS Hein JO David RH | 2008 | Eur Jobstet Gynecol Reprod Biology2008，138，： | 1 |
| 7 | Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater, marine and terrestrial ecosystems 显示文摘 | Elser JJ Bracken ME Cleland EE Gruner Daniel S Harpole WS Hillebrand H Ngai JT Seabloom EW Shurin JB Smith JE | 2007 | Ecol Lett2007，10，12： | 1 |
| 8 | Homocysteine-associated acute mesenteric artery occlusion treated with thrombectomy and bowel resection显示文摘 | Gradman WS Daniel J Miller B | 2001 | Ann Vase Surg2001，15，2： | 1 |
| 9 | Diurinal blood pressure variation in the evaluation of early onset severe pre-eclampsia显示文摘 | Daniel WS Hein JO David RH | 2008 | Eur J Obstet Gynecol Reppod Biology2008，138，： | 1 |
| 10 | Diurinal blood pressure variation in the evaluation of early onset severe pre-eclampsia显示文摘 | Daniel WS Hein JO David RH | 2008 | Eur J Obstet Gynecol Reprod Biology2008，138，： | 1 |
| 11 | Diurnal blood pressure variation in the evaluation of early onset severe preeclampsia 显示文摘 | Daniel WS Hein JO David RH | 2008 | Eur J Obstet Gy necol Reprod Biology2008，138，： | 1 |
| 12 | The Next Generation of E-Learning: Strategies for Media Rich Online Teaching and Engaged Learning 显示文摘 | Daniel T Lee CS Goh WS | 2004 | International Journal of Distance Education Technologies2004，2，4： | 1 |
| 13 | Value of primordial and primary prevention for cardiovascular disease: a policy state- ment {rom the American Heart Association显示文摘 | Weintraub WS Daniels SR Burke LE | 2011 | Circulation2011，124，8： | 1 |
| 14 | Value of primordial and primary prevention for cardiovascular disease: a policy state- ment from the American Heart Association 显示文摘 | Weintraub WS Daniels SR Burke LE | 2011 | Circulation2011，124，8： | 1 |
| 15 | A comparison of the CO2 laser and electrocautery on postoperative intraperitoneal adhesion formation in rabbits显示文摘 | Pittaway DE Maxson WS Daniell JF | 1983 | Fertil Steril1983，40，3： | 1 |
| 16 | Reducing the risk of mal- practice claims 显示文摘 | DANIEL ML BARRY JM DAVID WS | 1999 | Hospital Practice1999，34，7： | 1 |
| 17 | Reliability of radiographic intervertebral disc height measurement for in vivo rat - tail model显示文摘 | Alon Lai Daniel Chow Siu WS | 2007 | Medical Engineering & Physics2007，29，： | 1 |
| 18 | Analytical anion-exchange HPLC of recombinanttype-5 adenoviral particles 显示文摘 | Paul WS Daniel DG Ann MG | 1997 | Hum Gene Ther1997，3，1： | 1 |
| 19 | Gestational diabetes mellitus:is a diagnosis associated with an increase in maternal anxiety and stress in the short and intermediate term显示文摘 | Daniells S Grenyer BF Davis WS | 2003 | Diabetes Care2003，26，： | 1 |
| 20 | β_2-adrenoceptor in obstructive airway diseases:Agonism, antagonism or both?显示文摘Obstructive airway disease is a complex disease entity including several maladies characterized by bronchoconstriction and abnormal airway inflammation. Reversing bronchoconstriction leads to symptomatic relief and improvement in quality of life, both in reversible(bronchial asthma) and partially reversible(chronic obstructive airway disease) obstructive airway diseases. β2-adrenoceptor expressed in human airway is the main β-receptor subtype, and its activation in airway smooth muscle cells leads to bronchodilatation. Drugs targeting β-adrenoceptors have been around for many years, for which agonists of the receptors are used in bronchodilation while antagonists are used in cardiovascular diseases. This review article summarizes the effect and usage of β2-agonist in obstructive airway disease, addressing the benefits and potential risks of β2-agonist. The article also looks at the safety of β-blocker usage for cardiovascular disease in patients with obstructive airway disease. There is also emerging evidence that non-selective β-blockers with inverse agonism ironically can have longterm beneficial effects in obstructive airway disease that is beyond cardiovascular protection. Further trials are urgently needed in this area as it might lead to a dramatic turnaround in clinical practice for obstructive airway diseases as has already been seen in the usage of β-blockers for heart failure. | Daniel WS Tan Jyi Lin Wong Siew Teck Tie John A Abisheganaden Albert YH Lim WS Fred Wong | 2015 | World Journal of Respirology2015，5，3： | 0 |
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