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| 1 | Predictive value of Ki67 and p53 in locally advanced rectal cancer:Correlation with thymidylate synthase and histopathological tumor regression after neoadjuvant 5-FU-based chemoradiotherapy显示文摘AIM: To investigate the predictive value of Ki67 and p53 and their correlation with thymidylate synthase (TS) gene expression in a rectal cancer patient cohort treated according to a standardized recommended neoadjuvant treatment regimen.METHODS: Formalin fixed, paraffin embedded pre-therapeutical tumor biopsies (n = 22) and post-therapeutical resection specimens (n = 40) from patients with rectal adenocarcinoma (clinical UICC stage Ⅱ/Ⅲ) receiving standardized neoadjuvant 5-fluorouracil (5-FU) based chemoradiotherapy were studied for Ki67 and p53 expression by immunohistochemistry and correlated with TS mRNA expression by quantitative TaqMan real-time PCR after laser microdissection. The results were compared with histopathological tumor regression according to a standardized semiquantitative score grading system.RESULTS: Responders (patients with high tumor regression) showed a significantly lower Ki67 expression than non-responders in the pre-therapeutical tumor biopsies (81.2% vs 16.7%; P < 0.05) as well as in the post-therapeutical resection specimens (75.8% vs 14.3%; P < 0.01). High TS mRNA expression was significantly correlated with a high Ki67 index and low TS mRNA expression was significantly correlated with a low Ki67 index in the pre-therapeutical tumor biopsies (corr. coef. = 0.46; P < 0.01) as well as in the post-therapeutical resection specimens (corr. coef. = 0.40; P < 0.05). No significant association was found between p53 and TS mRNA expression or tumor regression.CONCLUSION: Ki67 has, like TS, predictive value in rectal cancer patients after neoadjuvant 5-FU based chemoradiotherapy. The close correlation between Ki67 and TS indicates that TS is involved in active cell cycle processes. | Christiane Jakob Torsten Liersch Wolfdietrich Meyer Heinz Becker Gustavo B Baretton Daniela E Aust | 2008 | World Journal of Gastroenterology2008，14，7： | 29 |
| 2 | Relationship between Crohn’s disease, infection with Mycobacterium avium subspecies paratuberculosis and SLC11A1 gene polymorphisms in Sardinian patients显示文摘AIM: To study the association between Crohn’s disease (CD), Mycobacterium avium subspecies paratubercu- losis (MAP), and genetic factors by examining the role of natural resistance-associated macrophage protein 1 (NRAMP1) gene polymorphisms (now SLC11A1) in Sar dinian patients with CD and controls. METHODS: Thirty-seven CD patients and 34 controls with no inflammatory bowel disease (IBD) were recruited at the University of Sassari after giving written con sent. Six SCL11A1 polymorphisms previously reported to be the most significantly associated with IBD were searched. M. paratuberculosis was identified by IS900 PCR and sequencing. Logistic regression was used to cal culate odds ratios (OR) for the associations among CD presence of MAP, and 6 loci described above. RESULTS: For the first time, a strong association was observed between polymorphisms at NRAMP1 locus 823C/T and CD. While CD was strongly associated withboth NRAMP1 and MAP, NRAMP1 polymorphisms and MAP themselves were not correlated. CONCLUSION: Combined with previous work on the NOD2/CARD15 gene, it is clear that the interplay of ge- netic, infectious, and immunologic factors in the etiology of CD is complex. | Leonardo A Sechi Maria Gazouli Lee E Sieswerda Paola Molicotti Niyaz Ahmed John Ikonomopoulos Antonio M Scanu Daniela Paccagnini Stefania Zanetti | 2006 | World Journal of Gastroenterology2006，12，44： | 3 |
| 3 | Cognitive disorder and dementia in type 2 diabetes mellitus显示文摘Insulin,a key pleiotropic hormone,regulates metabolism through several signaling pathways in target tissues including skeletal muscle,liver,and brain.In the brain,insulin modulates learning and memory,and impaired insulin signaling is associated with metabolic dysregulation and neurodegenerative diseases.At the receptor level,in aging and Alzheimer’s disease(AD)models,the amount of insulin receptors and their functions are decreased.Clinical and animal model studies suggest that memory improvements are due to changes in insulin levels.Furthermore,diabetes mellitus(DM)and insulin resistance are associated with age-related cognitive decline,increased levels ofβ-amyloid peptide,phosphorylation of tau protein;oxidative stress,pro-inflammatory cytokine production and dyslipidemia. Recent evidence shows that deleting brain insulin receptors leads to mildobesity and insulin resistance without influencing brain size and apoptosis development.Conversely, deleting insulin-like growth factor 1 receptor (IGF-1R) affects brain size anddevelopment, and contributes to behavior changes. Insulin is synthesized locally in the brain andis released from the neurons. Here, we reviewed proposed pathophysiological hypotheses toexplain increased risk of dementia in the presence of DM. Regardless of the exact sequence ofevents leading to neurodegeneration, there is strong evidence that mitochondrial dysfunctionplays a key role in AD and DM. A triple transgenic mouse model of AD showed mitochondrialdysfunction, oxidative stress, and loss of synaptic integrity. These alterations are comparable tothose induced in wild-type mice treated with sucrose, which is consistent with the proposal thatmitochondrial alterations are associated with DM and contribute to AD development. Alterationsin insulin/IGF-1 signaling in DM could lead to mitochondrial dysfunction and low antioxidantcapacity of the cell. Thus, insulin/IGF-1 signaling is important for increased neural processing andsystemic metabolism, and could be a specific target for therapeutic strategies to decreasealterations associated with age-related cognitive decline. | Genaro G Ortiz Miguel Huerta Héctor A González-Usigli Erandis D Torres-Sánchez Daniela LC Delgado-Lara Fermín P Pacheco-Moisés Mario A Mireles-Ramírez Blanca MG Torres-Mendoza Roxana I Moreno-Cih Irma E Velázquez-Brizuela | 2022 | World Journal of Diabetes2022，13，4： | 2 |
| 4 | Toxicity assessment of polyethylene microplastics in combination with a mix of emerging pollutants on Physalaemus cuvieri tadpoles显示文摘Studies in recent years have shown that aquatic pollution by microplastics(MPs)can be considered to pose additional stress to amphibian populations.However,our knowledge of how MPs affect amphibians is very rudimentary,and even more limited is our understanding of their effects in combination with other emerging pollutants.Thus,we aimed to evaluate the possible toxicity of polyethylene MPs(PE-MPs)(alone or in combination with a mix of pollutants)on the health of Physalaemus cuvieri tadpoles.After 30 days of exposure,multiple biomarkers were measured,including morphological,biometric,and developmental indices,behavioral parameters,mutagenicity,cytotoxicity,antioxidant and cholinesterase responses,as well as the uptake and accumulation of PE-MPs in animals.Based on the results,there was no significant change in any of the parameters measured in tadpoles exposed to treatments,but induced stress was observed in tadpoles exposed to PE-MPs combined with themixture of pollutants,reflecting significant changes in physiological and biochemical responses.Through principal component analysis(PCA)and integrated biomarker response(IBR)assessment,effects induced by pollutants in each test group were distinguished,confirming that the exposure of P.cuvieri tadpoles to the PE-MPs in combination with a mix of emerging pollutants induces an enhanced stress response,although the uptake and accumulation of PE-MPs in these animalswas reduced.Thus,our study provides newinsight into the danger to amphibians of MPs coexisting with other pollutants in aquatic environments. | Amanda Pereira da Costa Araújo Thiarlen Marinho da Luz Mohamed Ahmed Ibrahim Ahmed Mir Mohammad Ali Md.Mostafizur Rahman Bojan Nataraj Daniela de Melo e Silva DamiàBarceló Guilherme Malafaia | 2023 | Journal of Environmental Sciences2023，，5： | 2 |
| 5 | Nruroendovrine control of growth hormone secretion 显示文摘 | Eugenio E Muller Vittorio Locatelli Daniela Cocchi | 1999 | Physiol Rev1999，70，2： | 1 |
| 6 | The bronchiolar epithelium as a prominent source of pro-inflammatory cytokines after lung irradiation显示文摘 | CLAUDIA E R DANIELA U FALK W | | 0，，05： | 1 |
| 7 | The role C4 metabolism in the marine diatom Phaeodactylum tricornutum 显示文摘 | Maya H D Nitsan G Daniela E | 2013 | New Phytologist2013，197，： | 1 |
| 8 | Evaluation of Nonconventional Additives as Fire Retardants on Polya- mide 6,6: Phosphorous-based Master Batch, a-Zirconium Dihydrogen Phosphate, and b-Cyclodextrin Based Nano- sponges显示文摘 | Daniela E Jenny A Alberto F et aI | 2012 | Journal of Applied Polymer Science2012，123，： | 1 |
| 9 | Mineral composition and bitter pit severity in ‘Catarina' apples 显示文摘 | Cassandro V T A Daniela V C Paulo R E | 2006 | Rev Bras Frutic2006，28，1： | 1 |
| 10 | Ganglioneuroma of the adrenal gland 显示文摘 | Daniela S A Donna E H | 2009 | J Urology2009，，182： | 1 |
| 11 | Rosuvstatinreduces platelet activation in heart failure role of nobioavailability 显示文摘 | Andreas S Daniela F Martin E | 2005 | Arterioscler Thromb Vase BioU2005，25，5： | 1 |
| 12 | Induc'don of pro- inflammatory gene expression by Escberichia coli and mycotoxin zearalenone contamination and protection by a Lactobacillus mixture in porcine IPEC-I cells 显示文摘 | Ionelia T Daniela E M Gina C P e~ al | 2015 | Toxicon2015，97，4： | 1 |
| 13 | Cell-type and tissue-specific exprssion of caveolin-2, caveolins 1 and 2 colocalize and from a statble hetero-oligomeric complex invivo显示文摘 | Scherer Renee Y Lewis Daniela V Jeffrey A | 1997 | J Biol Chem1997，272，29： | 1 |
| 14 | Calculation of Modulus T of the Anomalous Magnetic Vector over Large Areas显示文摘 | Daniela G Sachio E | 2002 | Memoirs of the Faculty of Engineering2002，62，4： | 1 |
| 15 | Mycobacterium avium subspecies paratuberculosis infects and multiplies in enteric glial cells显示文摘AIM: To establish the role of enteric glial cells during infection with Mycobacterium avium subspecies paratuberculosis (MAP) in Crohn's disease. METHODS: In order to establish the role of enteric glial cells during infection with M. avium subspecies paratuberculosis (MAP) in Crohn's disease, Map adhesion experiments on enteric glial cells were performed as well as expression analysis of Map sigma factors during infection. RESULTS: In this study, for the first time, we found a high affinity of MAP to enteric glial cells and we analyzed the expression of MAP sigma factors under different conditions of growth. CONCLUSION: The fact that Map showed a high affinity to the glial cells raises concerns about the complicated etiology of the Crohn's disease. Elucidation of the mechanisms whereby inflammation alters enteric neural control of gut functions may lead to novel treatments for Crohn's disease. | Leonardo A Sechi Anne Ruehl Niyaz Ahmed Donatella Usai Daniela Paccagnini Giovanna E Felis Stefania Zanetti | 2007 | World Journal of Gastroenterology2007，13，43： | 1 |
| 16 | Preoperative Education in Cholecystectomy in the Context of a Multimodal Protocol of Perioperative Care: A Randomized, Controlled Trial显示文摘 | de Aguilar-Nascimento Jos 6 E Leal Fernando S Dantas Daniela C S Anabuki Nadia T de Souza Amanda M C Silva E Lima Ver6nica P Tanajura Guilherme H Canevari Mariana | 2013 | World journal of surgery2013，34，23： | 1 |
| 17 | Single-stage tubular urethral reconstruction using oral grafts is an alternative to classical staged approach for selected penile urethral strictures显示文摘Penile urethral strictures have been managed by a staged surgical approach.In selected cases,spongiofibrosis can be excised,a neo-urethral plate created using buccal mucosa graft(BMG)and tubularized during the same procedure,performing a“twoin-one”stage approach.We aim to identify stricture factors which indicate suitability for this two-in-one stage approach.We assess surgical outcome and compare with staged reconstruction.We conducted an observational descriptive study.The data were prospectively collected from two-in-one stage and staged penile urethroplasties using BMG in a single center between 2007 and 2017.The minimum follow-up was 6 months.Outcomes were assessed clinically,radiologically,and by flow-rate analysis.Failure was defined as recurrent stricture or any subsequent surgical or endoscopic intervention.Descriptive analysis of stricture characteristics and statistical comparison was made between groups.Of 425 penile urethroplasties,139 met the inclusion criteria:59 two-in-one stage and 80 staged.The mean stricture length was 2.8 cm(single stage)and 4.5 cm(staged).Etiology was lichen sclerosus(LS)52.5%(single stage)and 73.8%hypospadias related(staged).40.7%of patients had previous failed urethroplasties in the single-stage group and 81.2%in the staged.The most common stricture locations were navicular fossa(39.0%)and distal penile urethra(59.3%)in the single-stage group and mid or proximal penile urethra(58.7%)in the staged group.Success rates were 89.8%(single stage)and 81.3%(staged).A trend toward a single-stage approach for select penile urethral strictures was noted.We conclude that a single-stage substitution penile urethroplasty using BMG as a“two-in-one”approach is associated with excellent functional outcomes.The most suitable strictures for this approach are distal,primary,and LS-related strictures. | Felix Campos-Juanatey Simon Bugeja Mariya Dragova Anastasia V Frost Stella L Ivaz Daniela E Andrich Anthony R Mundy | 2020 | Asian Journal of Andrology2020，22，2： | 1 |
| 18 | Quality of Life, Psychosocial Well-Being, and Sexual Satisfaction in Women with Polycystic Ovary Syndrome显示文摘 | Sigrid E Susanne H Daniela K | 2003 | J Clin Endocrinol Metab2003，88，12： | 1 |
| 19 | The SUMO-E3 ligase PIAS3 targets pyruvate kinase M2显示文摘 | Spoden G A Dieter M Daniela E | 2009 | Journal of Cellular Biochemistry2009，107，2： | 1 |
| 20 | Control of membrane lipid fluidity by molecular thermosensors 显示文摘 | MARIA C M LARISA E C DANIELA A | 2004 | Journal of Bacteriology2004，186，20： | 1 |
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